Populations Of Receptor Neurons Research Articles

Spatiotemporal Coding of Individual Chemicals by the Gustatory System.

Our results provide a new view of taste processing. Using a new, relatively simple model system and a new set of techniques to deliver taste stimuli and to examine gustatory receptor neurons and their immediate followers, we found no evidence for labeled line connectivity, or basic taste categories such as sweet, salty, bitter, and sour. Rather, individual tastant chemicals are represented as patterns of spiking activity distributed across populations of receptor neurons. These representations are transformed substantially as multiple types of receptor neurons converge upon follower neurons, leading to a combinatorial coding format that uniquely, rapidly, and efficiently represents individual taste chemicals. Finally, we found that the information content of these neurons can drive tastant-specific behavior.

Odorant tuning of olfactory crypt cells from juvenile and adult rainbow trout

Teleost fish lack independent olfactory organs for odorant and pheromone detection. Instead, they have a single sensory epithelium with two populations of receptor neurons, ciliated and microvillous, that are conserved among vertebrates, and a unique receptor cell type named the olfactory crypt cell. Crypt cells were shown to be chemosensory neurons that project to specific areas in the olfactory bulb, but their odorant tuning and overall function remain unclear. Reproduction in fish is generally synchronized by sex pheromonal signaling between males and females, but the sensors responsible for pheromone detection remain unknown. In crucian carp, a seasonal variation in the population of olfactory crypt cells and their brain projections pathways, involved in reproduction, led to the hypothesis of a role as sex pheromone detectors. In the present study, morphology and localization of olfactory crypt cells were compared between juvenile and mature rainbow trout of both sexes, and calcium imaging was used to visualize responses of crypt cells from the three groups to common social and food-related odorants, sex hormones and conspecific tissue extracts. Crypt cells from mature trout were found to be larger than those of juvenile specimens, and preferentially localized to the apical surface of the olfactory epithelium. Although a fraction of crypt cells of all groups responded to common odorants such as amino acids and bile salts, cells from mature trout showed a characteristic preference for gonadal extracts and hormones from the opposite sex. These results support an involvement of olfactory crypt cells in reproduction-related olfactory signaling in fishes.

Anatomical Contributions to Odorant Sampling and Representation in Rodents: Zoning in on Sniffing Behavior

Odorant sampling behaviors such as sniffing bring odorant molecules into contact with olfactory receptor neurons (ORNs) to initiate the sensory mechanisms of olfaction. In rodents, inspiratory airflow through the nose is structured and laminar; consequently, the spatial distribution of adsorbed odorant molecules during inspiration is predictable. Physicochemical properties such as water solubility and volatility, collectively called sorptiveness, interact with behaviorally regulable variables such as inspiratory flow rate to determine the pattern of odorant deposition along the inspiratory path. Populations of ORNs expressing the same odorant receptor are distributed in strictly delimited regions along this inspiratory path, enabling different deposition patterns of the same odorant to evoke different patterns of neuronal activation across the olfactory epithelium and in the olfactory bulb. We propose that both odorant sorptive properties and the regulation of sniffing behavior may contribute to rodents' olfactory capacities by this mechanism. In particular, we suggest that the motor regulation of sniffing behavior is substantially utilized for purposes of "zonation" or the direction of odorant molecules to defined intranasal regions and hence toward distinct populations of receptor neurons, pursuant to animals' sensory goals.

Neural representation of sound amplitude by functionally different auditory receptors in crickets.

The physiological characteristics of auditory receptor fibers (ARFs) of crickets, a model system for studying auditory behaviors and their neural mechanisms, are investigated. Unlike auditory receptor neurons of many animals, cricket ARFs fall into three distinct populations based on characteristic frequency (CF) [Imaizumi and Pollack, J. Neurosci. 19, 1508-1516 (1999)]. Two of these have CFs similar to the frequency component of communication signals or of ultrasound produced by predators, and a third population has intermediate CF. Here, sound-amplitude coding by ARFs is examined to gain insights to how behaviorally relevant sounds are encoded by populations of receptor neurons. ARFs involved in acoustic communication comprise two distinct anatomical types, which also differ in physiological parameters (threshold, response slope, dynamic range, minimum latency, and sharpness of tuning). Thus, based on CF and anatomy, ARFs comprise four populations. Physiological parameters are diverse, but within each population they are systematically related to threshold. The details of these relationships differ among the four populations. These findings open the possibility that different ARF populations differ in functional organization.

The molecular logic of olfaction in Drosophila.

Drosophila fruit flies display robust olfactory-driven behaviors with an olfactory system far simpler than that of vertebrates. Endowed with 1300 olfactory receptor neurons, these insects are able to recognize and discriminate between a large number of distinct odorants. Candidate odorant receptor molecules were identified by complimentary approaches of differential cloning and genome analysis. The Drosophila odorant receptor (DOR) genes encode a novel family of proteins with seven predicted membrane-spanning domains, unrelated to vertebrate or nematode chemosensory receptors. There are on the order of 60 or more members of this gene family in the Drosophila genome, far fewer than the hundreds to thousands of receptors found in vertebrates or nematodes. DOR genes are selectively expressed in small subsets of olfactory neurons, in expression domains that are spatially conserved between individuals, bilaterally symmetric and not sexually dimorphic. Double in situ RNA hybridization with a number of pairwise combinations of DOR genes fails to reveal any overlap in gene expression, suggesting that each olfactory neuron expresses one or a small number of receptor genes and is therefore functionally distinct. How is activation of such a subpopulation of olfactory receptor neurons in the periphery sensed by the brain? In the mouse, all neurons expressing a given receptor project with precision to two of 1800 olfactory bulb glomeruli, creating a spatial map of odor quality in the brain. We have employed DOR promoter transgenes that recapitulate expression of endogenous receptor to visualize the projections of individual populations of receptor neurons to subsets of the 43 glomeruli in the Drosophila antennal lobe. The results suggest functional conservation in the logic of olfactory discrimination from insects to mammals.

Development of vomeronasal receptor neuron subclasses and establishment of topographic projections to the accessory olfactory bulb

Previous studies of the adult vomeronasal system have shown that vomeronasal receptor neurons in the middle layer (expressing Gi α2 ) and deep layers (expressing Go α ) of the sensory epithelium project to the anterior and posterior parts of the accessory olfactory bulb (AOB), respectively. In the present study, the development of the two populations of vomeronasal receptor neurons and their segregated projections were investigated in the opossum, Monodelphis domestica. Antibodies to G proteins Gi α2 and Go α were used to identify the two subpopulations of receptor neurons. The Gi α2 -immunoreactive (ir) cells and Go α -ir cells appeared between postnatal day 0 (P0) and postnatal day 3 (P3) and both types of cells increased in number during later development. The differential localization of Gi α2 -ir cells in the middle layer and Go α -ir cells in the deep layer of the VNO could be seen as early as P3 and became more prominent at later stages. The AOB was clearly identified at P10, and at this stage segregated projections of Gi α2 -ir fibers to the anterior part and Go α -ir fibers to the posterior part of the AOB were seen. The segregation of the two types of fibers in the AOB resemble that in the adult after P21. These results suggest that Gi α2 -ir and Go α -ir subpopulations of receptor neurons in the VNO develop in parallel, and that segregation of the two populations of receptor neurons in the VNO and the topographic projection to the AOB are established at very early stages during development.

Subclasses of vomeronasal receptor neurons: differential expression of G proteins (G iα2 and G oα) and segregated projections to the accessory olfactory bulb

Differential expression of G proteins (G iα2 and G oα) and the separate central projections of G iα2- and G oα-immunoreactive (ir) vomeronasal receptor neurons were investigated in the mouse and rat using immunocytochemical methods. In the vomeronasal organ (VNO), receptor neurons with their cell bodies located in the middle layer (middle 1/3) of the vomeronasal sensory epithelium express G iα2. Axons of these G iα2-ir neurons can be followed from VNO to the anterior part, but not the posterior part, of the nerve-glomerular (N-GL) layer of the accessory olfactory bulb (AOB). Another population of receptor neurons, which are located in the deep layer (basal 1/3) of the vomeronasal sensory epithelium, express G oα and axons of the G o α-ir neurons can be traced to the posterior part, but not the anterior part, of the N-GL layers of the AOB. The axons of the two subclasses of receptor neurons are intermingled near the VNO and become segregated as they enter the AOB. Removal of the AOB results in retrograde degeneration of both G iα2-ir and G oα-ir receptor neurons in the VNO. These results suggest that at least two subclasses of receptor neurons exist in the VNO: the G iα2-ir neurons in the middle layer and the GO.-it neurons in the deep layer of the VNO. The G iα2-ir neurons in the middle layer of the VNO project to the anterior part of the AOB, while the G oα-ir neurons in the deep layer of the VNO project to the posterior half of the AOB. These results are similar to our previous observations in the gray short-tailed opossum, suggesting that the existence of at least two subclasses of receptor neurons in the vomeronasal epithelium with differential projections to the AOB is a conserved feature among mammals.

An ultrastructural study of glomeruli associated with vomeronasal organs transplanted into the rat CNS.

Rat neonate vomeronasal organs were transplanted into the parietal cortex of littermates to examine their survival and the behavior of axon growth into the surrounding host brain parenchyma. After survival times of 10-100 days the brains were processed for ultrastructural examination. The transplanted vomeronasal organs (VNO) formed several vesicles lined with a sensory epithelium. From these sensory epithelia, VNO neurons leave the epithelium and enter the host brain. Transplant neurons grew axons that fasciculated into bundles surrounded by sheath cell processes and formed one or more fiber plexuses containing distinct globose or spherical-shaped glomerular-like structures. The glomeruli consisted of nerve terminals between which existed asymmetric synaptic contacts. Rarely did we observe clear reciprocal synapses. The glomeruli also contained terminals that showed signs of degeneration, such as increased density of the terminals, clumping of mitochondria and multivesicular bodies. The glomeruli were not partitioned or subdivided by glial septa; however, glial profiles were interspersed among the sensory terminals. Transplant glomeruli also lacked periglomerular cells and had no definitive glial envelope. These results suggest that glomerular formation is not dependent on dendrite contribution of second order neurons or glial support, but rather on a complementary population of receptor neurons.