Bat Populations Research Articles

Interspecific variation in lower temperature thresholds of an assemblage of wintering bats

Abstract Winter presents environmental and energetic challenges for temperate insectivorous bats as colder temperatures increase metabolic rates while simultaneously reducing resource availability. While bats in northern regions typically hibernate or migrate to circumvent these adverse conditions, there is growing evidence of winter bat activity as weather permits. Bats at lower latitudes may experience shorter, milder winters, increasing opportunities for activity. To better understand the relationship between ambient temperature and winter bat activity, we deployed acoustic detectors in central Louisiana and eastern Texas and examined data at 3 levels of biological organization: overall bat activity, species richness, and species-specific activity. Across 1,576 detector-nights, we recorded 37,435 bat passes. Bats responded positively to warmer temperatures but the temperature threshold for winter activity varied among species, ranging from 7.2 to 15.6 °C. Consequently, observed species richness increased at warmer ambient temperatures. With activity linked to environmental conditions in a species-specific manner, different subsets of the winter bat assemblage may be active from night to night. Additionally, our study adds to a rather limited body of literature of winter bat activity and provides a baseline for future studies as white-nose syndrome and climate change affect North American bat populations.

Assessment of virus and Leptospira carriage in bats in France.

With over 1,400 species worldwide, bats represent the second largest order of mammals after rodents, and are known to host major zoonotic pathogens. Here, we estimate the presence of pathogens in autochthonous bat populations. First, we set out to check our samples for PCR amplification efficiency by assessing the occurrence of inhibited PCR reactions from different types of bat samples with amplifying the housekeeping gene β-actin. Second, we investigated the presence of five targeted pathogens in a French bat population using PCR. We targeted viral RNA of Canine distemper virus, Alphacoronavirus, Lyssavirus, Rotavirus and bacterial Leptospira DNA. To do so, we screened for these viruses in bat faecal samples as well as in oropharyngeal swab samples. The presence of Leptospira was assessed in urine, kidney, lung and faecal samples. Results showed a frequency of inhibited reactions ranging from 5 to 60% of samples, varying according to the sample itself and also suspected to vary according to sampling method and the storage buffer solution used, demonstrating the importance of the sampling and storage on the probability of obtaining negative PCR results. For pathogen assessment, rotavirus and alphacoronavirus RNA were detected in Myotis myotis, Myotis daubentonii, Myotis emarginatus and Rhinolophus ferrumequinum bats. Rotaviruses were also detected in Barbastella barbastellus. The presence of alphacoronavirus also varied seasonally, with higher frequencies in late summer and October, suggesting that juveniles potentially play an important role in the dynamics of these viruses. Leptospira DNA was detected in M. myotis and M. daubentonii colonies. The 16S rRNA sequences obtained from Leptospira positive samples showed 100% genetic identity with L. borgpetersenii. Neither canine distemper virus nor lyssavirus RNA were detected in any of the tested samples. This study is the first to show the presence of Leptospira in autochthonous French bats in addition to coronavirus and rotavirus RNA previously reported in European autochthonous bats.

Contact-independent exposure to Rhodococcus rhodochrous DAP96253 volatiles does not improve the survival rate of Myotis lucifugus (little brown bats) affected by White-nose Syndrome.

Since the emergence of White-nose Syndrome, a fungal disease in bats, caused by Pseudogymnoascus destructans, hibernating populations of little brown bats (Myotis lucifugus) have declined by 70-90% within P. destructans positive hibernacula. To reduce the impact of White-nose Syndrome to North American little brown bat populations we evaluated if exposure to volatile organic compounds produced by induced cells from Rhodococcus rhodochrous strain DAP96253 could improve the overwinter survival of bats infected by P. destructans. Two simultaneous field treatment trials were conducted at natural hibernacula located in Rockcastle and Breckinridge counties, Kentucky, USA. A combined total of 120 little brown bats were randomly divided into control groups (n=60) which were not exposed to volatile organic compounds and treatment groups (n=60) which were exposed to volatile organic compounds produced by non-growth, fermented cell paste composed of R. rhodochrous strain DAP96253 cells. Cox proportional hazard models revealed a significant decreased survival at the Rockcastle field trial site but not the Breckinridge field site. At the Breckinridge hibernacula, overwinter survival for both treatment and control groups were 60%. At the Rockcastle hibernacula, Kaplan-Meier survival curves indicated significantly increased overwinter survival of bats in the control group (43% survived) compared to the treatment group (20% survived). Although complete inhibition of P. destructans by volatile organic compounds produced by induced R. rhodochrous strain DAP96253 cells was observed in vitro studies, our results suggest that these volatile organic compounds do not inhibit P. destructans in situ and may promote P. destructans growth.

BatTool: projecting bat populations facing multiple stressors using a demographic model

Bats provide ecologically and agriculturally important ecosystem services but are currently experiencing population declines caused by multiple environmental stressors, including mortality from white-nose syndrome and wind energy development. Analyses of the current and future health and viability of these species may support conservation management decision making. Demographic modeling provides a quantitative tool for decision makers and conservation managers to make more informed decisions, but widespread adoption of these tools can be limited because of the complexity of the mathematical, statistical, and computational components involved in implementing these models. In this work, we provide an exposition of the BatTool R package, detailing the primary components of the matrix projection model, a publicly accessible graphical user interface (https://rconnect.usgs.gov/battool) facilitating user-defined scenario analyses, and its intended uses and limitations (Wiens et al., US Geol Surv Data Release 2022; Wiens et al., US Geol Surv Softw Release 2022). We present a case study involving wind energy permitting, weighing the effects of potential mortality caused by a hypothetical wind energy facility on the projected abundance of four imperiled bat species in the Midwestern United States.

A decade of hibernating bat communities along the periphery of a region of white‐nose syndrome

AbstractLong‐term monitoring programs are necessary to assess populations for conservation planning and management decisions. Hibernating bats in North America have declined because of numerous natural and human‐induced disturbances. White‐nose syndrome (WNS) has become the most serious threat to North American cave‐dwelling bats, leading to significant population declines in several species. We examined trends in hibernating bat populations at 11 hibernacula in northern Georgia and Alabama, USA, from 2013–2022, beginning when WNS was first detected in the region. Although we observed interannual variation in numbers of the federally endangered gray bat (Myotis grisescens), mean counts remained stable over time. In contrast, the tricolored bat (Perimyotis subflavus) and the federally endangered northern long‐eared bat (M. septentrionalis) declined by >90% in the first 5 years after WNS detection in the region. Although no northern long‐eared bats have been reported since 2019, tricolored bat counts stabilized following initial declines. Understanding changes in bat populations as WNS continues to spread, and determining the extent of population declines, is necessary for making appropriate management decisions. Our findings elucidate the status of cave‐dwelling bat species along the periphery of the white‐nose syndrome endemic region and highlight the importance of monitoring bat communities on a regional scale to develop effective conservation strategies.

Bats of the Safi-Essaouira Provinces (Morocco): New Inputs to the Knowledge of Bat Populations of the Atlantic Littoral

In Morocco, investigations of bats are limited and fragmentary. The majority of studies were done in northern provinces close to the Mediterranean Sea and focused on the geographical distribution of bats. With the aim of overcoming these gaps, we used diurnal caves visits, hand nets and mist nets, and acoustic surveys to make an inventory of the Atlantic littoral of Safi-Essaouira provinces, which is considered one of the most neglected regions in the country. In total, five caves and nine foraging habitats were surveyed from 2020 to 2022. Our surveys highlighted the distribution of eleven bat species belonging to five families, of which Rhinolophus blasii and Tadarida teniotis species were newly recorded in the study area. Three caves, namely, Sbaa Fam, Haouya, and Sidi Benkrara were newly discovered and hosted medium-sized colonies of five species. They were important for bats during the daytime, hibernation, and breeding. In foraging habitats, 2650 passes of 10 species were recorded. Pipistrellus pipistrellus and Pipistrellus kuhlii were the most active, followed by Miniopterus schreibersii and Eptesicus isabellinus, while Rhinolophus ferrumequinum, Hipposideros tephrus, Rhinolophus mehelyi, T. teniotis, Myotis punicus, and R. blasii were less active. Olive groves were the most diverse habitat with 8 species, followed by Safi pinewood and degraded Argane forest with 4 species each and Sidi Abderrahmane lake with 3 species. The other habitats hosted only two species each. This paper is the first to highlight data on bats in the coastal areas of Morocco and North Africa. Our results contribute to improve knowledge of the annual cycle of these bats and fill the gaps related to their distribution in the coastal area of the Safi-Essaouira provinces.

Hibernation telomere dynamics in a shifting climate: insights from wild greater horseshoe bats.

Hibernation is linked with various hypotheses to explain the extended lifespan of hibernating mammals compared with their non-hibernating counterparts. Studies on telomeres, markers of ageing and somatic maintenance, suggest telomere shortening slows during hibernation, and lengthening may reflect self-maintenance with favourable conditions. Bats in temperate zones adjust body temperatures during winter torpor to conserve energy and exploit mild conditions for foraging. Climate change may impact the hibernation cycle of bats, but more research is needed regarding the role of telomeres in understanding their response to a changing climate. Here, relative telomere length (rTL) was measured in the long-lived greater horseshoe bat Rhinolophus ferrumequinum (n = 223 individuals) over three winters, considering climatic conditions. Cross-sectional analyses revealed between-individual variation in rTL with a strong year effect, likely linked to varying weather conditions and foraging success. Additionally, within-individual increases of rTL occurred in 51% of consecutive measurements, with evidence of increasing telomerase expression during hibernation in this species. These findings highlight the beneficial effects of hibernation on telomeres and potential consequences of changing climatic conditions for long-lived temperate bats. Understanding the interplay between hibernation, telomeres, and climate can provide insights into the adaptive capacity and survival of bat populations facing environmental challenges.

Is the ‘bat population’ an operational concept for statutory species conservation?

Is the ‘bat population’ an operational concept for statutory species conservation?

Synthesis of LQR Controller Based on BAT Algorithm for Furuta Pendulum Stabilization

In this study, a controller design method based on the LQR method and BAT algorithm is presented for the Furuta pendulum stabilization system. Determine the LQR controller, it is often based on the designer's experience or using trial and error to find the Q, R matrices. The BAT search algorithm is based on the characteristics of the bat population in the wild. However, there are advantages to finding multivariate objective functions. The BAT algorithm has an improvement for the LQR controller to optimize the linear square function with fast response time, low energy consumption, overshoot, and a small number of oscillations. Swarm optimization algorithms have advantages in finding global extrema of multivariate functions. Therefore, with a large number of elements of the Q and R matrices, they can also be quickly found and these matrices still satisfy the Riccati equation. The controller with optimal parameters is verified through simulation results with different scenarios. The performance of the proposed controller is compared with a conventional LQR controller and implemented on a real system.

Literature review of tri-colored bat natural history with implications to management

In the past decade, caverniculous bat populations have plummeted due to White-nose syndrome (WNS). Tri-colored bat (Perimyotis subflavus) populations have declined drastically in areas where WNS has been found, leading to the decision to protect tri-colored bats under the federal Endangered Species Act in the United States. At this time, there has not been a thorough review of the literature, nor a concise summary of the tri-colored bat’s life history, diet, threats, or habitat preferences. This absence creates more work for policy makers, federal “Take” permit applicants, and conservationists to find, access, and review critical details of tri-colored bats. A major point of confusion stems from the multiple common names and genera tri-colored bats have been classified under since it was first described a century and a half ago. To address the lack of concise summary, we scoured the scientific literature and compiled nearly a century of data to provide a robust review of the ecology, life history, winter and summer habitats, as well as created maps and figures showing counties where studies have occurred, white-nose syndrome is present, and where bats have been documented. Additionally, this paper highlights data gaps and suggests future research topics that may better inform conservation and management decisions for tri-colored bats.

A genome assembly of the Yuma myotis bat, Myotis yumanensis.

The Yuma myotis bat (Myotis yumanensis) is a small vespertilionid bat and one of 52 species of new world Myotis bats in the subgenus Pizonyx. While M. yumanensis populations currently appear relatively stable, it is one of 12 bat species known or suspected to be susceptible to white-nose syndrome, the fungal disease causing declines in bat populations across North America. Only two of these 12 species have genome resources available, which limits the ability of resource managers to use genomic techniques to track the responses of bat populations to white-nose syndrome generally. Here we present the first de novo genome assembly for Yuma myotis, generated as a part of the California Conservation Genomics Project. The M. yumanensis genome was generated using a combination of PacBio HiFi long reads and Omni-C chromatin-proximity sequencing technology. This high-quality genome is one of the most complete bat assemblies available, with a contig N50 of 28.03 Mb, scaffold N50 of 99.14 Mb, and BUSCO completeness score of 93.7%. The Yuma myotis genome provides a high-quality resource that will aid in comparative genomic and evolutionary studies, as well as inform conservation management related to white-nose syndrome.

Identification and characterization of novel alphacoronaviruses in Tadarida brasiliensis (Chiroptera, Molossidae) from Argentina: insights into recombination as a mechanism favoring bat coronavirus cross-species transmission.

Bats are reservoirs of various coronaviruses that can jump between bat species or other mammalian hosts, including humans. This article explores coronavirus infection in three bat species (Tadarida brasiliensis, Eumops bonariensis, and Molossus molossus) of the family Molossidae from Argentina using whole viral metagenome analysis. Fecal samples of 47 bats from three semiurban or highly urbanized areas of the province of Santa Fe were investigated. After viral particle enrichment, total RNA was sequenced using the Illumina NextSeq 550 instrument; the reads were assembled into contigs and taxonomically and phylogenetically analyzed. Three novel complete Alphacoronavirus (AlphaCoV) genomes (Tb1-3) and two partial sequences were identified in T. brasiliensis (Tb4-5), and an additional four partial sequences were identified in M. molossus (Mm1-4). Phylogenomic analysis showed that the novel AlphaCoV clustered in two different lineages distinct from the 15 officially recognized AlphaCoV subgenera. Tb2 and Tb3 isolates appeared to be variants of the same virus, probably involved in a persistent infectious cycle within the T. brasiliensis colony. Using recombination analysis, we detected a statistically significant event in Spike gene, which was reinforced by phylogenetic tree incongruence analysis, involving novel Tb1 and AlphaCoVs identified in Eptesicus fuscus (family Vespertilionidae) from the U.S. The putative recombinant region is in the S1 subdomain of the Spike gene, encompassing the potential receptor-binding domain of AlphaCoVs. This study reports the first AlphaCoV genomes in molossids from the Americas and provides new insights into recombination as an important mode of evolution of coronaviruses involved in cross-species transmission. IMPORTANCE This study generated three novel complete AlphaCoV genomes (Tb1, Tb2, and Tb3 isolates) identified in individuals of Tadarida brasiliensis from Argentina, which showed two different evolutionary patterns and are the first to be reported in the family Molossidae in the Americas. The novel Tb1 isolate was found to be involved in a putative recombination event with alphacoronaviruses identified in bats of the genus Eptesicus from the U.S., whereas isolates Tb2 and Tb3 were found in different collection seasons and might be involved in persistent viral infections in the bat colony. These findings contribute to our knowledge of the global diversity of bat coronaviruses in poorly studied species and highlight the different evolutionary aspects of AlphaCoVs circulating in bat populations in Argentina.

The Geographical Differences in the Bird Prey of the Great Evening Bat (Ia io)

Geographic variation in diet can indicate if species have unique ecological roles or feeding strategies in different environments. Bats have a unique nocturnal ecological niche and a diverse diet, making them an ideal model for studying changes in feeding. Some bats have adapted to reduced food resources caused by environmental changes and human disturbances by expanding their dietary ecological niche. As one of the typical species whose dietary niche has expanded from invertebrates (insects) to vertebrates (birds), the feeding habits of the great evening bat (Ia io) have attracted much attention from researchers. Unfortunately, little is known about how the diet of Ia io varies in different regions. Therefore, in this study, the diversity and composition of the bird prey of the great evening bat from two populations were examined using cytochrome C oxidase subunit I (COI) amplicon sequencing. The results showed that the great evening bat in both populations preyed on a variety of passerine birds, with the Phylloscopidae being the dominant family. Although most birds preyed upon by these two populations are the same, there are significant differences in predation on Cettiidae between the two populations. Both populations prey on migratory birds more than resident birds. Both populations of bats can prey on finch birds weighing more than 15 g, but they preyed on more birds weighing less than 10 g. The results of this study provide an important addition to the study of changes in bat diets under the conditions of expanding dietary ecological niches.

Do Bats Avoid the Urban Core in the Breeding Season? A Case Study from Temperate Latitudes

Seasonal utilization of urban areas by bats remains inadequately explored. This study aimed to comprehensively survey the species composition and population dynamics of bats during both the breeding season (May–July) and autumn migration season (August–September) within a large urban area, specifically Kharkiv city, Ukraine. We conducted multiyear data collection from 2014 to 2016, employing a combination of acoustic recordings, mist-netting, and registration of opportunistically found bats. The results revealed the identification of seven bat species using all methods (Eptesicus serotinus, Nyctalus noctula, N. leisleri, Pipistrellus pygmaeus, P. kuhlii, P. nathusii, and Vespertilio murinus), with notable differences in species composition between the studied periods. During the migration season, N. noctula was a numerically predominant species, while P. kuhlii and E. serotinus were the most abundant during the breeding season. The urban core bat population during the breeding season primarily consisted of males and solitary reproductive females, mainly represented by P. kuhlii and E. serotinus. Acoustic recording data indicated that N. noctula actively avoided the urban core during the breeding season, but was more common on the city periphery. In contrast, during the migration season, the city experienced a significant surge in bat abundance, both in general and specifically among noctule bats, with their numbers increasing tenfold compared to the breeding season. Moreover, a considerable number of young individuals were observed during the migration season. These findings provide evidence that bats tend to avoid the urban core in large cities at temperate latitudes during the breeding season but actively utilize urban areas during autumn migration. Understanding the seasonal preferences and movements of bats in urban environments is crucial for effective conservation and management strategies.

The phenomenon of bat populations as a characteristic of watansoppeng city as a biology learning resource for students on ecosystem materials

This research is a case study research using qualitative descriptive research methods. The purpose of this study is to describe the phenomenon of the Bat population in Watansoppeng City and describe the benefits of research results from the Bat population phenomenon in Watansoppeng City to be used as a source for learning Ecosystem Biology. This research was conducted at the Environmental Service of Soppeng Regency and in Botto Village, Lalabata District, Soppeng Regency. The informants of this research were 7 people, namely the Head of the Soppeng Regency Environmental Service, the Secretary of the Soppeng Regency Environmental Service and 5 people from the community. Determination of informants in this study using a purposive sampling technique. Data collection techniques used are observation, interviews and documentation by using two types of data, namely primary data and secondary data. Primary data comes from interviews and direct observations at the research site, while secondary data comes from literature review. The data analysis used is data reduction, data presentation and conclusion drawing/verification. Test the validity of the data used is triangulation. The results obtained are the phenomenon of the Bat population in Soppeng Regency which has existed for a long time due to several factors such as habitat suitability, adequate food sources, protected and preserved so that bats can live and be well preserved. Thus, the phenomenon of the Bat population as a characteristic of the city of Watansoppeng can be used as a source of learning ecosystem material biology.

Using iNaturalist to monitor the roosting behavior of bats in Panama

Chiropterans are important mammals of conservation concern across Neotropical regions, facing a number of threats, including increasing levels of urbanization, leading to habitat loss. Despite this, many species of bats regularly utilize a variety of roosting sites, including both natural and man-made structures. Methods for monitoring bat populations include citizen science applications. The roosting habits of tropical bats has seldom been assessed on the most widespread citizen science app, iNaturalist. To this end, I characterized images of observations of bats in Panama. I report on 344 observations, with 59.9% roosting in natural habitats versus 29.6 % roosting in man-made environments, respectively. The most commonly observed species include Rhynchonycteris naso, Uroderma bilobatum, and Artibeus jamaicensis. Total observations increased annually, indicating the potential for future conservation monitoring. I recommend bat researchers utilize iNaturalist as a tool to study roosting selection and habitat use by bats, as this application is likely to increase in use.

Insights into the habitat associations, phylogeny, and diet of Pipistrellus maderensis in Porto Santo, northeastern Macaronesia

Abstract. Around 60 % of all bat species occur in islands, and nearly one in four is an insular endemic. Bats are often the only native terrestrial mammals in oceanic islands, and despite increasing anthropogenic pressures, little is known about the distribution, natural history, and population status of most insular bat populations. The sub-tropical archipelago of Madeira is composed of the volcanic islands of Madeira, Porto Santo, and Desertas and is home to the Macaronesian endemic Pipistrellus maderensis, to the endemic subspecies Nyctalus leisleri verrucosus, and to Plecotus austriacus. Pipistrellus maderensis is known to both Madeira and Porto Santo, whereas the other two species have only been recorded in the former. However, no bats have been recorded in Porto Santo for over 15 years, raising fears that bats are probably extinct in the island. In July 2021, we conducted an island-wide acoustic survey using AudioMoth passive acoustic recorders, leading to the detection of Pipistrellus maderensis in 28 out of the 46 sampling sites (60 %). The species' activity was strongly associated with artificial water sources, and genetic samples from six captured individuals revealed that the populations of Pipistrellus maderensis in Porto Santo and Madeira have a close phylogenetic affinity. Furthermore, using DNA metabarcoding, we found that the species feeds on a wide variety of insects, including several economically important pest species and disease vectors. These findings emphasise the need to target more conservation and research efforts towards extant island bat populations and the potential ecosystem services they provide.

Continuous low-intensity predation by owls (Strix aluco) on bats (Nyctalus lasiopterus) in Spain and the potential effect on bat colony stability.

Owls prey on bats, but information on owl predation is scarce, its impact on bat mortality is unclear, and reports on behavioural responses, including roost-switching and fission-fusion behaviour, are equivocal. To study the link between owl predation and anti-predator behaviour in bats, we evaluated seven months of video recordings at roosts and the behaviour of 51 passive integrated transponder (PIT)-tagged bats and bats without tags in a geographically isolated colony of greater noctule bats (Nyctalus lasiopterus) in Spain. We found the tawny owl Strix aluco to almost continuously hunt N. lasiopterus, from perches and on the wing, well after the bats emerged at dusk and when they returned to their roosts. We recorded 39 unsuccessful and three successful attacks. Nonetheless, we found no evidence that owl predation modifies bat behaviour. While the bats constituted only a very small proportion of the owls' diet, owl predation accounted for an estimated 30-40% of bat mortality, which may have a significant impact on small, local or isolated bat populations, in particular, and thereby shape regional bat distributions. We hypothesize that low roost availability may also affect the bats' potential response to predation, which could lead to natural predation having an excessive impact on bat populations.

Effective conservation of subterranean-roosting bats.

Bats frequently inhabit caves and other subterranean habitats and play a critical role in subterranean food webs. With escalating threats to subterranean ecosystems, identifying the most effective measures to protect subterranean-roosting bats is critical. We conducted a meta-analysis to evaluate the effectiveness of conservation and management interventions for subterranean-roosting bats. We used network analyses to determine to what extent interventions for bats overlap those used for other subterranean taxa. We conducted our analyses with data extracted from 345 papers recommending a total of 910 conservation interventions. Gating of roost entrances was applied to preserve bat populations in 21 studies, but its effectiveness was unclear. Habitat restoration and disturbance reduction positively affected bat populations and bat behavior, respectively, in ≤4studies. Decontamination was assessed in 2 studies and positively affected bat populations, particularly in studies focused on reducing fungal spores associated with white-nose syndrome in North America. Monitoring of bat populations as an effective conservation strategy was unclear and infrequently tested. Only 4% of bat studies simultaneously considered other subterranean organisms. However, effective interventions for bat conservation had similarities with all other organisms. If other subterranean organisms are considered when applying interventions to conserve bats, they might also benefit.

Damage on DNA and hematological parameters of two bat species due to heavy metal exposure in a nickel-mining area in central Brazil.

Exposure to heavy metals in mining zones is a significant threat, which can affect ecosystem services and contribute to the decline of wild bat populations. The present study investigated the impacts caused by mining on two bat species in central Brazil, the nectarivorous Glossophaga soricina and the frugivorous Carollia perspicillata. The bats were collected from a nickel-mining zone (treatment) and a protected area (control). The leukocyte profile of each species was compiled and genotoxicity (comet assay) and mutagenicity (micronucleus test) were determined using the appropriate procedures. Glossophaga soricina presented significantly higher frequencies of eosinophils and monocytes in the mining zone in comparison with the protected area, whereas C. perspicillata presented higher frequencies of lymphocytes in the mining zone, but significantly lower frequencies of monocytes. Concomitantly, G. soricina also presented a higher frequency of DNA damage, although no variation was found in this parameter in C. perspicillata when comparing environments. We also found no significant differences between populations in terms of the frequency of micronuclei and other nuclear abnormalities. Overall, the results of the study indicate that bats are susceptible to immunological disorders and DNA damage in mining zones, with the nectarivorous G. soricina appearing to be relatively more susceptible and thus a potentially effective bioindicator of the impact of contamination in these environments.