Refugial Populations Research Articles

Population genetic structure of the bank vole Myodes glareolus within its glacial refugium in peninsular Italy

It is now well established that Southern European peninsulas have been major glacial refugia for temperate species during Pleistocene climatic oscillations. However, substantial environmental changes occurred also within these peninsulas throughout the Pleistocene, rising questions about the role and interplay of various of micro-evolutionary processes in shaping patterns of intraspecific diversity within these areas. Here, we investigate the patterns of genetic variation in the bank vole Myodes glareolus within the Italian peninsula. By using a panel of 13 microsatellite loci, we found more intra-specific variation than expected based on previous assessments. Indeed, both Bayesian and ordination-based clustering analyses of variation recovered five main geographic/genetic clusters along the peninsula, with three clusters geographically restricted to the southern portion of the study area. This pattern supports the occurrence of multiple sub-refugia for the bank vole in peninsular Italy, likely promoted by the major paleo-environmental changes which affected forested habitats within this area during the Pleistocene. Thus, our results support a scenario whereby the high levels of intraspecific diversity observed within major glacial refugia are better explained by dynamic micro-evolutionary processes occurred within these areas, rather than by long-term demographic stability of refugial population. Finally, the narrow and isolated distribution of some of the identified lineages, suggest the need for future assessments of their conservation and taxonomic status.

Genomic evidence of survival near ice sheet margins for some, but not all, North American trees

Temperate species experienced dramatic range reductions during the Last Glacial Maximum, yet refugial populations from which modern populations are descended have never been precisely located. Climate-based models identify only broad areas of potential habitat, traditional phylogeographic studies provide poor spatial resolution, and pollen records for temperate forest communities are difficult to interpret and do not provide species-level taxonomic resolution. Here we harness signals of range expansion from large genomic datasets, using a simulation-based framework to infer the precise latitude and longitude of glacial refugia in two widespread, codistributed hickories (Carya spp.) and to quantify uncertainty in these estimates. We show that one species likely expanded from close to ice sheet margins near the site of a previously described macrofossil for the genus, highlighting support for the controversial notion of northern microrefugia. In contrast, the expansion origin inferred for the second species is compatible with classic hypotheses of distant displacement into southern refugia. Our statistically rigorous, powerful approach demonstrates how refugia can be located from genomic data with high precision and accuracy, addressing fundamental questions about long-term responses to changing climates and providing statistical insight into longstanding questions that have previously been addressed primarily qualitatively.

Out of the Mediterranean? Post‐glacial colonization pathways varied among cold‐water coral species

AbstractAimTo infer cold‐water corals’ (CWC) post‐glacial phylogeography and assess the role of Mediterranean Sea glacial refugia as origins for the recolonization of the northeastern Atlantic Ocean.LocationNortheastern Atlantic Ocean and Mediterranean Sea.TaxonLophelia pertusa, Madrepora oculata.MethodsWe sampled CWC using remotely operated vehicles and one sediment core for coral and sediment dating. We characterized spatial genetic patterns (microsatellites and a nuclear gene fragment) using networks, clustering and measures of genetic differentiation.ResultsInferences from microsatellite and sequence data were congruent, and showed a contrast between the two CWC species. Populations of L. pertusa present a dominant pioneer haplotype, local haplotype radiations and a majority of endemic variation in lower latitudes. Madrepora oculata populations are differentiated across the northeastern Atlantic and genetic lineages are poorly admixed even among neighbouring sites.ConclusionsOur study shows contrasting post‐glacial colonization pathways for two key habitat‐forming species in the deep sea. The CWC L. pertusa has likely undertaken a long‐range (post‐glacial) recolonization of the northeastern Atlantic directly from refugia located along southern Europe (Mediterranean Sea or Gulf of Cadiz). In contrast, the stronger genetic differentiation of M. oculata populations mirrors the effects of long‐term isolation in multiple refugia. We suggest that the distinct and genetically divergent, refugial populations initiated the post‐glacial recolonization of the northeastern Atlantic margins, leading to a secondary contact in the northern range and reaching higher latitudes much later, in the late Holocene. This study highlights the need to disentangle the influences of present‐day dispersal and evolutionary processes on the distribution of genetic polymorphisms, to unravel the influence of past and future environmental changes on the connectivity of cosmopolitan deep‐sea ecosystems associated with CWC.

Colonization of Northern Europe by Zygaena filipendulae (Lepidoptera).

Northern and mountainous ice sheets have expanded and contracted many times due to ice ages. Consequently, temperate species have been confined to refugia during the glacial periods wherefrom they have recolonized warming northern habitats between ice ages. In this study, we compare the gene CYP405A2 between different populations of the common burnet moth Zygaena filipendulae from across the Western Palearctic region to illuminate the colonization history of this species. These data show two major clusters of Z. filipendulae populations possibly reflecting two different refugial populations during the last ice age. The two types of Z. filipendulae only co‐occur in Denmark, Sweden, and Scotland indicating that Northern Europe comprise the hybridization zone where individuals from two different refugia met after the last ice age. Bayesian phylogeographic and ecological clustering analyses show that one cluster probably derives from an Alpe Maritime refugium in Southern France with ancestral expansive tendencies to the British Isles in the west, touching Northern Europe up to Denmark and Sweden, and extending throughout Central Europe into the Balkans, the Peleponnes, and South East Europe. The second cluster encompasses East Anatolia as the source area, from where multiple independent dispersal events to Armenia, to the Alborz mountains in north‐western Iran, and to the Zagros mountains in western Iran are suggested. Consequently, the classical theory of refugia for European temperate species in the Iberian, Italian, and Balkan peninsulas does not fit with the data from Z. filipendulae populations, which instead support more Northerly, mountainous refugia.

Filling the gap: the common hamster, Cricetus cricetus, phylogeography – a case study of Ukraine as potential refugial area

The phylogeographic analyses of the common hamster revealed the existence of five mitochondrial DNA (mtDNA) lineages. However, the analyses did not include Ukraine and the area outside Carpathian arch located in Romania that is important for the species as presumably refugial territory. We investigated both areas and described phylogeographic relationships of the populations on the basis of a partial cytochrome b (cytb, 904 bp) region of the mtDNA. Haplotype network and gene tree analyses did not produce a clear phylogeographic pattern for investigated territories that is typical for refugial populations. The highest diversity was found at the left bank of the River Dnieper and the area was called the Dnieper Lowland Refugial Area. Moreover only three from five described phylogeographic lineages (North, Pannonia and Caucasus) were fully separate on the network and gene trees. The haplotypes of other two lineages described previously from Eastern Europe (E1 and E0 lineages) mixed together with haplotypes from Ukraine and Moldovian Plateau. This study highlights the need for a re-examination of the phylogeography divisions of the common hamster.

Phylogeography of Potamon ibericum (Brachyura: Potamidae) identifies Quaternary glacial refugia within the Caucasus biodiversity hot spot.

Refugia are critical for the maintenance of biodiversity during the periods of Quaternary climatic oscillations. The long‐term persistence of refugial populations in a large continuous refugium has resulted in a homogenous pattern of genetic structure among populations, while highly structured evolutionary lineages characterize the restriction of refugial populations to smaller subrefugia. These mechanisms have resulted in the identification of hot spots of biodiversity within putative glacial refugia. We studied phylogeography of Potamon ibericum (Brachyura: Potamidae) in the drainages of the western Caucasus biodiversity hot spot (i.e., Colchis and the Caucasus) to infer spatial genetic structure and potential refugia for a freshwater crab in this region. These areas have traditionally considered as a refugium due to the presence of Tertiary relict species. We integrated population genetic data and historical demographic analysis from cytochrome oxidase subunit I sequences and paleoclimatic data from species distribution modeling (SDM). The results revealed the lack of phylogeographic structure and provided evidence for demographic expansion. The SDM presented a rather homogenous and large refugium that extended from northeast Turkey to Colchis during the last glacial period. In contrast to these findings, previous phylogeographic study on P. ibericum of the eastern Caucasus biodiversity hot spot (i.e., Hyrcania) identified multiple independent refugia. By combining these results, we explain the significance of this important western Palearctic hot spot of biological diversity in shaping the geographic distribution of intraspecific genetic diversity in a freshwater taxon.

Invasive species and postglacial colonization: their effects on the genetic diversity of a Patagonian fish.

The present distribution of Patagonian species is the result of a complex history involving Quaternary refugial populations, Holocene range expansions and demographic changes occurring during the Anthropocene. Invasive salmonids were introduced in Patagonia during the last century, occupying most rivers and lakes, preying on and competing with native species, including the fish Galaxias platei. Here, we used G. platei as a case study to understand how long-term (i.e. population differentiation during the Holocene) and short-term historical processes (salmonid introductions) affect genetic diversity. Using a suite of microsatellite markers, we found that the number of alleles is negatively correlated with the presence of salmonids (short-term processes), with G. platei populations from lakes with salmonids exhibiting significantly lower genetic diversity than populations from lakes without salmonids. Simulations (100 years backwards) showed that this difference in genetic diversity can be explained by a 99% reduction in population size. Allelic richness and observed heterozygosities were also negatively correlated with the presence of salmonids, but also positively correlated with long-term processes linked to Quaternary glaciations. Our results show how different genetic parameters can help identify processes taking place at different scales and their importance in terms of conservation.

Rethinking long‐term vegetation dynamics: multiple glacial refugia and local expansion of a species complex

Evidence is accumulating that some arcto‐boreal plant taxa persisted through the last glacial maximum (LGM) in Alaska and adjacent Canada. However, the spatial patterns of glacial persistence and associated postglacial colonization remain largely unknown. In this study, we investigated the LGM refugia of an alder (Alnus) species complex (n = 3 taxa) and assess the spatiotemporal dynamics of Alnus in this vast region. Specifically, we conducted high‐throughput DNA sequencing (ddRADseq) on Alnus foliar samples collected from a dense population network to investigate patterns of genetic structure and infer the presence of glacial lineages. Species distribution modeling (SDM) was used to investigate the probability and possible locations of glacial persistence. These analyses were integrated and then compared with fossil pollen data to identify the locations of refugial populations and spatial patterns of postglacial colonization. Our genetic analyses revealed two glacial lineages with separate geographic origins for each Alnus taxon, suggesting that the genus persisted in multiple LGM refugia. Non‐overlapping hindcast distributions based on SDMs further support the presence of multiple, spatially distinct refugia. These ddRADseq and SDM results, in conjunction with reassessment of fossil pollen records, suggest that Alnus expanded from several population nuclei that existed during the LGM and coalesced during the Holocene to form its present range. These results challenge the unidirectional model for postglacial vegetation expansion, implying that climate buffering associated with landscape heterogeneity and adaptation to millennial‐scale environmental variability played important roles in driving late‐Quaternary population dynamics.

Pockets of resistance: Response of arid‐land fish communities to climate, hydrology, and wildfire

Abstract Disturbance regimes of arid regions throughout the world are changing along with global warming. Severity of drought associated with decreased stream discharge and increased frequency and intensity of wildfires are increasing in many of these systems. Combined, these factors can have potentially devastating effects on stream fish communities. To quantify the response of fish communities to annual variation in climate, hydrology, and wildfire, we used long‐term and spatially extensive datasets from the Gila River drainage, NM, U.S.A. Additionally, we tracked the recovery of multiple fish communities within the study area following extreme drought and associated wildfires that occurred between 2011 and 2013. Twenty‐nine years of monitoring at six sites, beginning in 1988, indicated that fish community richness and density were negatively associated with drought, as quantified by the Palmer Drought Severity Index. Ordination of fish species densities across these sites further suggested that native species were more sensitive to inter‐annual variation in climate than non‐native fishes. The resistance and resilience of fishes to drought and ash flows following the most severe series of wildfires on record between 2011 and 2013 were highly variable across sites and species. The percent of common species that were able to resist a significant decline ranged from 0 to 70%, depending on location. As of 2017, all but two species (one native and one non‐native) had recovered at one or more sites. Although we hypothesised that the response to wildfire and drought that occurred between 2011 and 2013 should vary with body size and species provenance (native or non‐native), this was not supported. Rather, resistance of fish communities to these events appeared to be driven primarily by spatial factors, such as intensity of disturbance, and resilience was driven by species ability to rapidly rebound from severely depressed numbers or immigrate from nearby refuge populations. Current climate trends suggest an increase in the intensity of disturbance associated with wildfire and drought; thus, maintenance of native biodiversity should focus on preservation of habitat integrity and connectivity among habitats that allows for recolonisation of disturbed habitats.

Northern quoll persistence is most sensitive to survivorship of juveniles

Context Projecting the population trajectory of endangered species using models requires conservation practitioners to evaluate how variations in life history parameters may change a population’s viability. This is particularly important for species that occur as fragmented populations and whose densities are naturally low. Simulations may be used to identify conservation actions that have a higher likelihood of reducing a species’ extinction risk. Aims The aim was to apply population viability models to the northern quoll (Dasyurus hallucatus) under alternative scenarios. Methods The current (baseline) northern quoll demographic trajectories were evaluated using field-collected data derived from monitoring programs. The impact of alternative scenarios of mortality (for example, due to increased predation by introduced predators) and population supplementation (either from populations elsewhere or from captive breeding) on the viability of a northern quoll population was then determined. Key results Under current conditions, individual Pilbara populations of northern quoll are projected to persist for over 20 years. However, these populations are sensitive to extinction events. Population growth rate and local extinction risk were most sensitive to changes in juvenile mortality as low as 5% per annum. Increased mortality of the juvenile age cohort above current levels resulted in a projected decline in population size of 22–54%, with a moderate-to-high chance (20–96%) of local extinction within 20 years. Supplementing the population produced a moderate increase in quoll persistence over this time period. Conclusions Populations of northern quolls in the Pilbara, and potentially elsewhere in their range, are highly sensitive to even small perturbations in juvenile mortality rates. The continued persistence of quoll populations in fragmented refuges is characteristic of a species that functions as a dynamic metapopulation in the face of high environmental perturbations. Implications Increased juvenile mortality above current levels – for example through the spread of cane toads or invasion of feral cats – may have serious implications for the persistence of the current network of northern quoll populations and other mammals that exhibit population fragmentation in arid environments. Estimates of survival rates for the juvenile cohort of quolls would improve diagnosis of a species’ population dynamics as well as inform practitioners of key life-history sensitivities.

The establishment of Scotland’s rarest freshwater fish, the vendace (Coregonus albula), in conservation refuge sites

In recent decades conservation measures for the rarest freshwater fish in the U.K., the vendace (Coregonus albula), have included attempts to form conservation refuge populations in Scotland. Here we report that at two of these refuge sites where the status of the introduced vendace was previously unknown (Loch Earn and Daer Reservoir) surveys have established that vendace are reproducing successfully in situ, albeit that these populations appear to be relatively numerically small. At a third site, Loch Valley, there was no evidence of vendace in the survey, but it is possible that a small, but as yet undetectable population is in the process of establishing.

Spatial genetic subdivision among populations of Pampus chinensis between China and Pakistan: testing the barrier effect of the Malay Peninsula

Tissue samples from 84 Pampus chinensis individuals were collected from four geographic regions within the Indo–Pacific Ocean and analyzed using mitochondrial and nuclear DNA markers. Distinct genetic heterogeneity was found for both types of markers between Chinese and Pakistani populations, while the diversity of this species was high in all populations. In combination with published information on marine species with similar distributions, these results suggested that the Malay Peninsula, or a less effective supplement, played a role in shaping the contemporary genetic structure. This population structure was presumably reflected in P. chinensis, whose populations were genetically isolated during Pleistocene glaciations and then did not experience secondary contact between previous refuge populations. However, P. chinensis showed genetic continuity in China or Pakistan, which indicated that the populations in different geographical regions constituted a single panmictic stock with high gene flow, respectively. The spatial genetic subdivision evident among populations indicates that P. chinensis in this Indo–Pacific region should be managed as different independent stocks to guide the sustainability of this fisheries resource.

Phylogeographic analyses of a widely distributed Populus davidiana: Further evidence for the existence of glacial refugia of cool-temperate deciduous trees in northern East Asia.

Despite several phylogeographic studies had provided evidence to support the existence of glacial refugia of cool‐temperate deciduous trees in northeast China, the species used in these studies were limited by the species ranges, which could not exclude the possibility that northern populations were the colonists from southern refugial populations during the last glacial maximum (LGM). Here, we estimated the nucleotide variation in Populus davidiana, a widespread species distributed in Eurasia. Three groups in northeast, central, and southwest China were constructed according to the simulation results from SAMOVA, composition of chloroplast haplotypes and structure results. We revealed that the northeast China had endemic haplotypes, the haplotypes and nucleotide diversity in northern regions were not lower than that in southern China, and this species has not experienced population expansion base on the estimation of Bayesian skyline plots. Ecological niche modeling (ENM) indicated that the northeast China had a high suitability score during the last glacial maximum. The combined evidence clearly demonstrated that northeastern and southwestern refugia were maintained across the current distributional range of P. davidiana during the LGM. The genetic differentiation between these two refugia might be mainly caused by differences of climate among these areas. The phylogeographic analyses of a widely distributed P. davidiana provided robust evidence to clarify the issue of refugia in northeast China, and these results are of great importance for understanding the influence of Quaternary glaciations on the distribution and evolution of species in East Asia.

Considerations for the Use of Captive-Reared Delta Smelt for Species Recovery and Research

Author(s): Lessard, JoAnna; Cavallo, Brad; Anders, Paul; Sommer, Ted; Schreier, Brian; Gille, Daphne; Schreier, Andrea; Finger, Amanda; Hung, Tien-Chieh; Hobbs, James; May, Bernie; Schultz, Andrew; Burgess, Oliver; Clarke, Robert | Abstract: An extreme decline in Delta Smelt (Hypomesus transpacificus) abundance has led to a number of management actions to support this endangered species, including the development and refinement of culture techniques and the creation of a refuge population. The wild Delta Smelt population has diminished to the point that many in the scientific community believe population supplementation using cultured fish needs to be experimentally evaluated as a possible management tool. Concerns about supplementation include the effectiveness of this action, and its potential to divert attention and funding from other needed management actions such as habitat restoration. Here, we describe the outcomes of a 2-day workshop that described the current refuge population, and identified key issues for potential future use of cultured Delta Smelt for research and management. Expanded use of cultured Delta Smelt is controversial and requires consideration for complexities that include legal constraints and permitting requirements. Developing policies that allow for in situ experiments using cultured Delta Smelt appears to be a precursor for advancing policies that might allow supplementation actions. Releases of cultured fish, either experimentally or as a management action, clearly need to be conducted within an adaptive management program that is integrated with other strategies, including habitat restoration. We describe a general framework for evaluating the potential risks of supplementation and include suggestions for how to reduce risks and uncertainty. Overall, we conclude there is sufficient baseline information about Delta Smelt and the existing culture program to proceed with targeted field research that utilizes cultured fish. Finally, given the dire status of this species, we conclude that rapid progress toward the development of a viable and testable supplementation program must be a priority for Delta Smelt conservation.

A test of the European Pleistocene refugial paradigm, using a Western Palaearctic endemic bird species.

Hewitt's paradigm for effects of Pleistocene glaciations on European populations assumes their isolation in peninsular refugia during glacial maxima, followed by re-colonization of broader Europe during interstadials. This paradigm is well supported by studies of poorly dispersing taxa, but highly dispersive birds have not been included. To test this paradigm, we use the dunnock (Prunella modularis), a Western Palaearctic endemic whose range includes all major European refugia. MtDNA gene tree, multilocus species tree and species delimitation analyses indicate the presence of three distinct lineages: one in the Iberian refugium, one in the Caucasus refugium, and one comprising the Italian and Balkan refugia and broader Europe. Our gene flow analysis suggests isolation of both the Iberian and Caucasus lineages but extensive exchange between Italy, the Balkans and broader Europe. Demographic stability could not be rejected for any refugial population, except the very recent expansion in the Caucasus. By contrast, northern European populations may have experienced two expansion periods. Iberia and Caucasus had much smaller historical populations than other populations. Although our results support the paradigm, in general, they also suggest that in highly dispersive taxa, isolation of neighbouring refugia was incomplete, resulting in large super-refugial populations.

Microclimatic gradients provide evidence for a glacial refugium for temperate trees in a sheltered hilly landscape of Northern Italy

ABSTRACTAimRefugia play a key role in conserving biodiversity during periods of unfavourable and highly variable regional climate. However, refugial populations are often small and fragmented, which makes their identification difficult. In this study, we investigate whether an area of complex topography in the southern foreland of the Alps could have provided a suitable microclimate to serve as a glacial refugium for temperate trees during the last glacial.LocationThe Euganean Hills in Northern Italy (Veneto).MethodsWe assessed the current microclimatic variability in the ecologically diverse region on a fine scale by recording half‐hourly near‐surface temperatures over a period of 11 months. After comparing our measurements with today's vegetation distribution, broad‐scale synoptic patterns, and topoclimatic factors, we estimated refugial suitability based on extreme temperatures, climatic stability, and difference from the regional average.ResultsPresent‐day temperature gradients within the Euganean Hills are large enough to explain the presence of the temperate tree species Fagus sylvatica as well as Mediterranean Quercus ilex, two species that are absent elsewhere in the adjacent Po Plain. During winter, anticyclonic weather patterns resulted in strong atmospheric inversions, with temperatures increasing by +1°C/100 m in the hills relative to the surrounding Po Plain.Main conclusionsOur high‐resolution climate data support multi‐proxy palaeoecological records identifying the Euganean Hills as a refugium for temperate trees. Temperature anomalies of 2–4°C relative to the surrounding Po Plain are sufficient to allow several temperate tree species to survive the Last Glacial Maximum (LGM) in the Euganean Hills under a harsh continental climate. Specifically, elevations >200 m a.s.l. in the central parts of the hills may have provided suitable conditions for local LGM refugia. Regions of complex topography such as the Euganean Hills conserved past and present biodiversity and provide high‐priority areas for conservation under future climate warming.

Experimental Manipulation of Dispersal Ability in A Neotropical Butterfly Anartia fatima (Lepidoptera: Nymphalidae).

Research on endangered British butterflies has found that butterfly populations in small refuges evolve to allocate more mass to the thorax (flight muscle) and less to the abdomen than populations in large refuges. The observed change in mass allocation affects two morphological features relevant to flight: the flight muscle ratio (FMR) and the position of center of body mass (cmbody). The author tested whether a decrease in FMR or a change in cmbody reduced the ability to disperse by experimentally weight-loading Neotropical Anartia fatima butterflies. In one treatment group, FMR was decreased but cmbody was not altered, whereas in the second group FMR was decreased and cmbody was repositioned further posterior. In one mark–release–recapture (MRR) experiment, butterflies dispersed relatively slowly, and treatment groups did not differ significantly. In a replicate experiment, butterflies dispersed more quickly, and control butterflies dispersed more rapidly than either treatment group. Differences in dispersal were consistent with a causal relationship between FMR and movement. A more posterior cmbody had little effect on dispersal beyond that due to the change in FMR. These results support the hypothesis that an increase in mass allocation to the thorax in small, dispersed refugia is due to selection on the ability to disperse.

Oxygen consumption of desert pupfish at ecologically relevant temperatures suggests a significant role for anaerobic metabolism.

Oxygen consumption is oftentimes used as a proxy for metabolic rate. However, pupfish acclimated to ecologically relevant temperatures may employ extended periods of anaerobism despite the availability of oxygen-a process we called paradoxical anaerobism. In this study, we evaluated data from pupfish exhibiting stable oxygen consumption. Routine oxygen consumption ([Formula: see text]) of a refuge population derived from Cyprinodon spp. acclimated to 28 and 33°C was evaluated at the ecologically relevant assay temperatures of between 25 and 38°C. Different interpretations of the data are available depending on normalization. For instance, [Formula: see text] of smaller fish, measured per fish, was remarkably stable over a wide range of assay temperatures and was not different between acclimation groups. However, when measured on a mass-specific basis, [Formula: see text] in these same smaller fish increases more predictably as temperature increased. [Formula: see text] of refuge fish and the closely related pupfish, C. nevadensis mionectes, measured near their respective acclimation temperatures, were essentially identical. However, [Formula: see text] of 28°C acclimated fish of both species, when measured at 34°C, was greater than that of the 33°C acclimated fish measured at 28°C. We suggest that this observed 'efficiency' may result from significant anaerobic metabolism use. Experiments investigating factorial aerobic scope ([Formula: see text]/[Formula: see text]) yielded values less than 1 in 21-36% of the 33°C acclimated fish. These values indicate a substantial contribution of anaerobic metabolism to energy utilization by these fish. However, muscle lactate levels are not elevated in exercising fish-a result that is consistent with paradoxical anaerobism use.

Live weight as a basis for targeted selective treatment of lambs post-weaning

Targeted selective treatment (TST) has been proposed as a sustainable method of gastrointestinal nematode control that reduces the number of anthelmintic treatments administered, thereby preserving a susceptible nematode population in refugia. In order to minimise the impact of withholding treatment on animal performance, animals that would benefit most should be selected for treatment. However, the most suitable criteria for selecting which animals to treat remain a subject of research. The impact of implementing a TST strategy based on lamb live weight was investigated, and whether heavy lambs were more resilient than light lambs if left untreated. The study was conducted using weaned lambs on 3 sheep farms, and over 2 years. On each farm lambs were weighed and divided into heavy, medium and light weight classes. Within the heavy (n = 225) and light (n = 218) weight classes lambs were randomly allocated to two treatments; anthelmintic treatment or no anthelmintic treatment. All lambs in the medium weight class were treated. Animal performance and parasitological parameters were assessed over a 28-day period. Anthelmintic treatment had a significant effect (P < 0.01) on faecal egg count, average daily weight gain, body condition score and dag score; there was no treatment by weight class interaction. Anthelmintic treatment had no effect on plasma pepsinogen concentration or on blood leukocyte numbers. Withholding anthelmintic treatment from lambs therefore had a negative effect on worm egg count and animal performance with no evidence that heavy lambs were more resilient than light lambs when left untreated.

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

Background: Gastrointestinal nematodes are one of the major health and economic problem of sheep and goats in the world. The control of these nematodes is carried out conventionally with synthetic anthelminths, which favored the selection of gastrointestinal nematode (GIN) populations multiresistant to anthelmintics. The emergence of anthelmintic resistance has stimulated the search for new alternatives to control small ruminant GIN, standing out the use of plants and their bioactives compounds, such as essential oils (EO). The objective of this review was to present the main characteristics and anthelmintic activity of EO, their isolated compounds and drug delivery systems in the control of GIN.Review: Essential oils are a complex blend of bioactive compounds with volatile, lipophilic, usually odoriferous and liquid substances. EO are composed of terpenes, terpenoids, aromatic and aliphatic constituents. EO has various pharmacological activities of interest in preventive veterinary medicine such as antibacterials, antifungals, anticoccicids, insecticides and anthelmintics. In vitro and in vivo tests are used to validate the anthelmintic activity of EO on GIN. In vitro tests are low cost screening tests that allow the evaluation of the anthelmintic activity of a large amount of bioactive compounds on eggs, first (L1) and third stage larvae (L3), and adult nematodes. The antiparasitic effect of EO is related to its main compound or to the interaction of the compounds. These bioactive compounds penetrate the cuticle of the nematodes by transcuticular diffusion, altering the mechanisms of locomotion, besides causing cuticular lesions. Following in vitro evaluation, the acute and sub-chronic toxicity test should be performed to assess the toxicity of the bioactive compounds and to define the dose to be used in in vivo tests. In vivo tests are more reliable because the anthelmintic effectiveness of bioactive compounds is evaluated after the metabolization process. The metabolization process of the bioactive compounds can generate metabolites that exhibit or not anthelmintic effectiveness. The in vivo tests assessing the anthelmintic effectiveness of bioactive compounds in sheep and goats are the fecal egg count reduction test and the controlled test. OE promoted reduction of egg elimination in faeces which may be related to cuticular and reproductive alterations in GIN, and reduction of parasite burden in in vivo tests. Due to the promising results obtained with OE in the in vivo tests, interest has been aroused in using nanotechnology as an alternative to increase the bioavailability of OE and consequently, potentializing its anthelmintic effect, reducing the dose and toxicity of the biocompounds. In addition to nanotechnology, the isolation and chemical modification of compounds isolated from OE have been employed to obtain new molecules with anthelmintic action and understand the mechanism of action of EO on the small ruminant GIN.Conclusion: The use of EO and their compound bioactive in the control of resistant populations of GIN is a promising alternative. The adoption of strategies in which natural products can replace synthetic anthelmintics, such as in dry periods and use synthetic anthelmintics in the rainy season when the population in refugia in the pasture is high, thus reducing the dissemination of GIN resistant populations. As perspective, the evaluation of pharmacokinetics and pharmacodynamics of these natural products should be performed so that one defines treatment protocols that optimize the anthelmintic effect.