Population Loss Research Articles

Türkiye'de Kırsal Mimarlığın ve Yaşamın Korunması Üzerine Kritik Bir İnceleme: Vizyonlar, Araçlar ve Kuruluşlar

Once a vibrant fabric of life, rural Turkey is facing a significant problem with population loss today. With changes in policies and the declining attractiveness of rural areas, rural architecture and lifestyles are in danger of disappearing. This study explores the complexities of this crisis, going beyond the mere preservation of buildings. It analyzes agricultural, legal, and administrative policies that trigger migration from rural areas and examines international frameworks. Beyond preserving buildings, the study delves into the initiatives, responses, and values that are critical to the revitalization of rural life. The study assesses movements that have been initiated to empower local communities and sustain rural lifestyles, recognizing that the sustainability of rural life is dependent on a thriving community. In conclusion, safeguarding Turkey's rural heritage requires a transformative approach. By prioritizing local participation, fostering interconnected solutions, and addressing the existing limitations, a future of thriving rural communities and their cultural heritage becomes possible.

Physical activity intensity classification during activities of daily living in older adults using accelerometers: Is the ear the new wrist?

Introduction
 Accurate measurement of physical activity (PA) in older adults is important, both in health research and personalized prevention. Accelerometers, used to overcome the limitations of self-reporting, were initially worn on the hips, but are increasingly worn on the non-dominant wrist. While this can improve wear compliance, the accuracy of PA intensity classification can be compromised. Given the high prevalence of mild to severe hearing loss in the older population, this study explores a novel approach: integrating an accelerometer into a hearing aid (ear sensor). We aimed to assess its accuracy and compare it to research-grade sensors worn at different locations.
 Methods 60 middle-aged to older adults (64.0 ± 8.0 years, 48% women) were included in this study. Each subject performed 12-13 different activities, which were pseudo-randomly selected from a list of 33 activities of daily living. Each activity lasted 8 min and included sedentary activities (e.g., lying, playing cards) low-intensity activities (e.g., hanging laundry), activities of changing intensity or without physical displacement (e.g., yoga, squats), indoor activities related to locomotion (e.g., walking, running), outdoor activities (e.g., walking uphill, cycling), and activities with aids (e.g., walking with a stroller). Oxygen consumption was measured via indirect calorimetry and used to classify activity intensity into sedentary behavior (SB, metabolic equivalent of task [MET] < 1.5), light intensity PA (LPA, 1.5 ≤ MET < 3.0), or moderate to vigorous intensity PA (MVPA, MET ≥ 3.0). The ear sensor was placed behind the left ear, while the research-grade sensors were placed on both wrists and ankles, on the hip, chest, and forehead. Estimation of PA intensity classes was done using mean amplitude deviations and ROC analyses. Contingency tables were used to determine classification accuracy.
 Results
 Overall accuracy of the ear sensor was 82.6%, performing better than both wrists (left 81.1%, right 76.0%) and both ankles (left 81.1%, right 81.9%), but worse than the forehead (83.6%), hip (85.6%) and the chest (85.9%). ROC analyses show that all sensors can effectively discriminate between sedentary vs. non-sedentary activities (AUC 0.97-0.98, exception ankles: AUC 0.95-0.96) and between MVPA vs. other (AUC 0.96-0.97, exception wrists: AUC 0.89-0.92).
 Discussion/Conclusion
 This study is the first to show that an accelerometer integrated into a hearing aid can accurately classify PA intensity and differentiate MVPA and sedentary behavior in older adults. It also confirms previous investigations showing that wrist-worn sensors – although increasingly being used to monitor PA – are less effective in capturing MVPA compared to sensors worn closer to the center of mass (including the head/ear in our study). Although the optimal wear site in older adults is a subject of ongoing debate, our data shows that a sensor integrated into a hearing aid offers a promising balance of classification accuracy and (possibly) user compliance. Further studies should explore integrating in-ear heart rate monitoring to enhance accuracy even further.

The Demographic Factor in Shaping State Policy for Higher Education of Ukraine

The article examines key indicators of the demographic crisis in Ukraine, highlighting manifestations of the nation’s aging and natural population decline.It’s determined that understanding and analyzing the demographic situation allows for predicting changes in the size and structure of the student population, which is crucial for the effective development of higher education in Ukraine. Considering the trends of declining birth rates and an aging population, it is important to adapt admission strategies for students and educational programs accordingly. The examination of the demographic crisis helps identify key challenges, such as a decrease in the number of high school graduates, which can impact competition within the higher education environment.The trends examined in the article, including the decline in the birth rate of the Ukrainian population, the aging of the nation, the level of migration, and the loss of the civilian population due to the war, enable us to characterize the situation in Ukraine as depopulation. Notable changes in the demographic landscape and shifts in the population structure occurred after February 24, 2022, including irreversible human losses, population emigration (over 10 million people), and a decline in the average birth rate. A correlation was identified between the number of births each year and the dynamics of educational services’ capacity. It was observed that higher education in Ukraine will confront significant demographic challenges in the next 17 years. In the context of the strategic goal to restore Ukraine’s human capital, the article suggests viewing higher education as an effective instrument of state policy in a cyclical process: attaining higher education, integrating graduates into the national economy, ensuring financial stability, fostering active citizenship, and promoting loyalty to the state—all contributing to an improvement in the demographic situation.

A Physics-Based Seismic Risk Assessment of the Qujiang Fault: From Dynamic Rupture to Disaster Estimation

This study achieved the construction of earthquake disaster scenarios based on physics-based methods—from fault dynamic rupture to seismic wave propagation—and then population and economic loss estimations. The physics-based dynamic rupture and strong ground motion simulations can fully consider the three-dimensional complexity of physical parameters such as fault geometry, stress field, rock properties, and terrain. Quantitative analysis of multiple seismic disaster scenarios along the Qujiang Fault in western Yunnan Province in southwestern China based on different nucleation locations was achieved. The results indicate that the northwestern segment of the Qujiang Fault is expected to experience significantly higher levels of damage compared to the southeastern segment. Additionally, there are significant variations in human losses, even though the economic losses are similar across different scenarios. Dali Bai Autonomous Prefecture, Chuxiong Yi Autonomous Prefecture, Yuxi City, Honghe Hani and Yi Autonomous Prefecture, and Wenshan Zhuang and Miao Autonomous Prefecture were identified as at medium to high seismic risks, with Yuxi and Honghe being particularly vulnerable. Implementing targeted earthquake prevention measures in Yuxi and Honghe will significantly mitigate the potential risks posed by the Qujiang Fault. Notably, although the fault is within Yuxi, Honghe is likely to suffer the most severe damage. These findings emphasize the importance of considering rupture directivity and its influence on ground motion distribution when assessing seismic risk.

Lactylation-driven FTO targets CDK2 to aggravate microvascular anomalies in diabetic retinopathy

Diabetic retinopathy (DR) is a leading cause of irreversible vision loss in working-age populations. Fat mass and obesity-associated protein (FTO) is an N6-methyladenosine (m6A) demethylase that demethylates RNAs involved in energy homeostasis, though its influence on DR is not well studied. Herein, we detected elevated FTO expression in vitreous fibrovascular membranes of patients with proliferative DR. FTO promoted cell cycle progression and tip cell formation of endothelial cells (ECs) to facilitate angiogenesis in vitro, in mice, and in zebrafish. FTO also regulated EC-pericyte crosstalk to trigger diabetic microvascular leakage, and mediated EC–microglia interactions to induce retinal inflammation and neurodegeneration in vivo and in vitro. Mechanistically, FTO affected EC features via modulating CDK2 mRNA stability in an m6A-YTHDF2-dependent manner. FTO up-regulation under diabetic conditions was driven by lactate-mediated histone lactylation. FB23-2, an inhibitor to FTO’s m6A demethylase activity, suppressed angiogenic phenotypes in vitro. To allow for systemic administration, we developed a nanoplatform encapsulating FB23-2 and confirmed its targeting and therapeutic efficiency in mice. Collectively, our study demonstrates that FTO is important for EC function and retinal homeostasis in DR, and warrants further investigation as a therapeutic target for DR patients.

Biological Containment for African Swine Fever (ASF) Laboratories and Animal Facilities: The Italian Challenge in Bridging the Present Regulatory Gap and Enhancing Biosafety and Biosecurity Measures

The African Swine Fever Virus (ASFV) is a DNA virus of the Asfarviridae family, Asfivirus genus. It is responsible for massive losses in pig populations and drastic direct and indirect economic impacts. The ever-growing handling of ASFV pathological material in laboratories, necessary for either diagnostic or research activities, requires particular attention to avoid accidental virus release from laboratories and its detrimental economic and environmental effects. Recently, the Commission Delegated Regulation (EU) 2020/689 of 17 December 2019 repealed the Commission Decision of 26 May 2003 reporting an ASF diagnostic manual (2003/422/EC) with the minimum and supplementary requirements for ASF laboratories. This decision generated a regulatory gap that has not been addressed yet. This paper aims to describe the Italian National Reference Laboratory (NRL) efforts to develop an effective and reliable biological containment tool for ASF laboratories and animal facilities. The tool consists of comprehensive and harmonized structural and procedural requirements for ASF laboratories and animal facilities that have been developed based on both current and repealed legislation, further entailing a risk assessment and internal audit as indispensable tools to design, adjust, and improve biological containment measures.

Global irreparable population losses and economic damage reported by method, strategy, and target of terrorist attack

Relevance. To reduce the consolidated budget burden, public administration both in Russia and abroad is pursuing optimal solutions. In turn, identification of the most effective approaches to eliminating terrorist attack consequences is implemented by all concerned departments, agencies and services, with reimbursement of damages incurred by terrorist attacks among the goals. By analyzing retrospective data on the reported incurred damage of different scale, medical specialists and health care authorities can justify medical and economic feasibility of efforts to adapt preparedness to the elimination of the medical and sanitary consequences of terrorist attacks.The objective is to identify in terms of incurred damage the most dangerous methods, strategies, and targets to perform terrorist attacks.Methods. This study relies on a generalized database of terrorist attack consequences taken place from 1970 through 2020, and reported by various experts. The retrospective analysis included more than 220,000 cases of terrorist actions; the study key parameters were identified to include terrorist attack methods, strategies, and targets.Results and analysis. The study results show that depending on the scale of damage over years, the share of terrorist attacks associated with fatal outcome is characterized by a general upward trend. Terrorist attacks by explosion remains the most significant in terms of frequency of cases and share among other methods. Individuals are top one target of terrorist attacks by the number of cases. Business community representatives bear the utmost share of major consequences.

OP17 IBD ulcers are characterized by bioactive interleukin-1 and transcriptomic hallmarks of stromal cell state reprogramming

Abstract Background The programs that perpetuate the inflammation and prevent epithelial repair in Inflammatory Bowel Disease (IBD) remain unclear. Interleukin (IL)-1 plays a role in the maintenance of mucosal homeostasis but also in IBD. Expansion of IL-1 expressing myeloid cells is a hallmark of IBD tissue, including severe and anti-TNF unresponsive disease, and IL-1 expression is highly localized to ulcer beds. However, little is known about the presence of bioactive IL-1 proteins in the cell-free mucosal environment of IBD, nor whether IL-1-driven programs affect epithelial regeneration in IBD ulcers. Methods We established a highly sensitive assay (~500-fold greater than ELISA) to assess bioactive extracellular IL-1 from previously cryopreserved IBD biopsies. We assessed a cohort of Crohn’s Disease (CD, n=23), Ulcerative Colitis (UC, n=18) and non-IBD (n=17) patients, with biopsies from paired inflamed/uninflamed regions (primarily colon but also ileal). We assessed IL-1 bioactivity, including IL-1α vs IL-1β contributions, and performed RNA-seq of samples’ matched cellular compartments. An ulcer-associated gene signature was interrogated in a single-cell (sc)RNA-seq cohort (n=42) of very early onset (VEO)IBD including monogenic disorders, as well as publicly available IBD bulk RNA-seq datasets and scRNA-seq of mouse models of colitis. Results IL-1α and IL-1β bioactivity corresponded with disease and ulcer severity in CD and UC. The most extreme signals were seen in select CD patients with deep ulceration. IL-1α was the predominant contributor to total IL-1 bioactivity in most patients although several with ulcers displayed IL-1β predominance. IL-1 bioactivity correlated with IL-1 transcripts in matched RNA-seq, and weighted gene co-expression network analysis revealed a compelling ulcer-specific module. This module contained transcription factors and genes related to cell state, validated in publicly available datasets (e.g. RISK cohort of ileal CD and scRNA-seq of murine DSS colitis), that when interrogated in a scRNA-seq dataset of VEOIBD (including patients with IL-10RA mutations characterized by deep ulcers), suggests an orchestrated IL-1-driven myeloid/stromal response to epithelial ulceration involving cell state reprogramming and loss of key myofibroblast populations. Conclusion Mucosal ulceration in IBD is associated with bioactive IL-1α and IL-1β proteins, and transcriptomic evaluation of the same correlates with bioactive signal. An ulcer-associated gene module, validated in other datasets, sheds light on IL-1 biology in intestinal epithelial repair. Results strongly suggest the IL-1 signaling pathway being an attractive and precision-based therapeutic target in subsets of IBD patients.

MPZL2—a common autosomal recessive deafness gene related to moderate sensorineural hearing loss in the Chinese population

BackgroundMutations in MPZL2, the characteristic genetic etiology of autosomal recessive deafness loci 111 (DFNB111), cause non-syndromic and moderate sensorineural hearing loss.MethodsIn this study, we analyzed the phenotype and genotype of eight pedigrees consisting of 10 hearing loss patients with bi-allelic pathogenic or likely pathogenic variants in MPZL2. These patients were identified from a 3272 Chinese patient cohort who underwent genetic testing.ResultsApart from symmetrical and moderate sensorineural hearing loss, the MPZL2-related phenotype was characterized by progressive hearing loss with variation in the onset age (congenital defect to onset at the young adult stage). We determined that in the Chinese population, the genetic load of MPZL2 defects was 0.24% (8/3272) in patients diagnosed with hearing loss and 7.02% (8/114) in patients diagnosed with hereditary moderate sensorineural hearing loss caused by STRC, OTOA, OTOG, OTOGL, TECTA, MPZL2 and others. Three known MPZL2 variants (c.220C > T (p.Gln74*), c.68delC (p.Pro23Leufs*2), c.463delG (p.Ala155Leufs*10)) and a novel start loss variant (c.3G > T (p.Met1?)) were identified. MPZL2 c.220C > T was identified as the hotspot variant in the Chinese population and even in East Asia compared with c.72delA (p.Ile24Metfs*22) in European and West Asia through allele frequency.ConclusionsWe concluded that apart from moderate HL, progressive HL is another character of MPZL2-related HL. No specified variant was verified for the progression of HL, the penetrance and expressivity cannot be determined yet. A novel MPZL2 variant at the start codon was identified, enriching the variant spectrum of MPZL2. The hotspot variants of MPZL2 vary in different ethnicities. This study provides valuable data for the diagnosis, prognosis evaluation and genetic counseling of patients with moderate sensorineural hearing loss related to MPZL2.

Assessment of Efficacy of Algerian Propolis against the Parasitic Mite Varroa destructor and Safety for Honey Bees by Spray Treatment.

Varroa destructor is an ectoparasitic mite and is considered one of the most important causes of honey bee population loss. In the last years, substances of botanical origin have emerged as natural alternatives to diminish the mite population levels. Propolis is a natural product and is used by honey bees for multiple tasks, including protection from pathogens and parasites, and varroacidal activity of propolis extracts has been shown. In this study, we investigated the potential of propolis, collected by native Algerian honey bee subspecies (Apis mellifera intermissa and A. m. sahariensis) in different locations in Algeria and extracted by ultrasound, to control mites of V. destructor and tested the safety for the honey bees. The most important results were that the best propolis extracts at 10% killed 100% of the Varroa mites within 3-4 h in a Petri dish assay. In addition, when we sprayed A. m. intermissa bees infested with Varroa mites with a 10% concentration in a mini-hive setup, we scored a high mite mortality of 85-87% with the best propolis extracts, and importantly, there was no mortality in the bees. Our data demonstrated that propolis extracts in Algeria could be used in honey bee colonies by spraying against Varroa mite infestations, which may develop as an easy method for local beekeepers to control Varroa in their hives. Further research should investigate the mechanism of action.

Relationship Between Hearing Loss and Cognitive Function in Elderly Chinese People: A Study Based on Propensity Score Matching

To explore the relationship between hearing loss and cognitive function in the elderly population through propensity score matching method. We analyzed the data of 7605 participants aged 60 and above who were included in the 2018 China Health and Retirement Longitudinal Study (CHARLS). The non-substitutable 1∶1 nearest neighbor matching method without caliper value was used for propensity score matching and G-computation was used to estimate the average treatment effect (ATE) of hearing loss on all dimensions of cognitive function. Before matching, there were 3626 (47.68%) women, with 1409 (18.53%) of whom suffering from hearing loss and 3031 (39.86%) of whom suffering from cognitive impairment. After matching, 1409 subjects were included in the hearing loss group and 1409, in the normal hearing group, with both groups sharing similar distribution of basic demographic characteristics. The results for the average treatment effect of the population indicated that the cognitive function scores of the hearing loss group were lower than those of the normal hearing group, with the overall cognitive function being 0.593 points lower (95% confidence intervel [CI]: －0.916-－0.257, P<0.001), orientation being 0.183 points lower (95% CI: －0.302-－0.055, P=0.004), immediate memory being 0.150 points lower (95% CI: －0.218-－0.085, P<0.001), and language skills being 0.178 points lower (95% CI: －0.303-－0.058, P=0.006). The prevalence of cognitive impairment of the hearing loss group was 4.2% higher than that of the normal hearing group (95% CI: 0.007-0.077, P=0.020). Hearing loss adversely affects the orientation, memory, and language skills of the elderly population and forms a potential risk factor for cognitive impairment in the elderly population.

Modeling the Simultaneous Dropout of Energetic Electrons and Protons by Magnetopause Shadowing

AbstractMagnetopause shadowing (MPS) effect could drive a concurrent dropout of radiation belt electrons and ring current protons. However, its relative role in the dropout of both plasma populations has not been well quantified. In this work, we study the simultaneous dropout of MeV electrons and 100s keV protons during an intense geomagnetic storm in May 2017. A radial diffusion model with an event‐specific last closed drift shell is used to simulate the MPS loss of both populations. The model well captures the fast shadowing loss of both populations at L* &gt; 4.6, while the loss at L* &lt; 4.6, possibly due to the electromagnetic ion cyclotron wave scattering, is not captured. The observed butterfly pitch angle distributions of electron fluxes in the initial loss phase are well reproduced by the model. The initial proton losses at low pitch angles are underestimated, potentially also contributed by other mechanisms such as field line curvature scattering.

Freshwater fish parasites in Temengor, Chenderoh and Bersia reservoirs, Perak, Malaysia

Parasitic infection causes a decrease in wild fish population and financial losses in culture fish.Fundamental biological variables like fish parasite composition baseline data and fish hosts parasites interactions on fish parasites are needed in establishing a monitoring andmanagement system of these freshwater fishes to mitigate the possible adverse effect of theseparasitic diseases. There is no information about protozoa and myxozoa parasitic fauna of thefishes in River Perak reservoirs. The prevalence of freshwater fish parasites in Temengor, Bersia and Chenderoh Reservoirs, Perak was investigated for 23 months for fish parasites using standard parasitological techniques. A total of 32 species of parasites detected belonging to 8 major taxa: 2 species of protozoans, 2 species of myxozoa, 12 species of monogeneans, 3 species of digeneans, 4 species of nematodes, 3 species of acanthocephalans and 5 species of copepods. Balantidium sp., Henneguya sp., and Myxobulus sp. were newly recorded parasites species in the reservoirs. The prevalence of parasitic infection in the reservoirs were 51 % in Temengor, Bersia 61% and Chenderoh 65 %. The parasite diversity of the fish parasites per reservoir were moderate. The parasite with the highest prevalence rate was recorded in Paradiplozoon barbi (17.39 %). Eighteen fish species were infected with parasites out of the twenty-five species sampled from the reservoirs. The fish family Cyprinidae was the dominant and most parasitized fish family in the reservoirs.

COVID-19-related health utility values and changes in COVID-19 patients and the general population: a scoping review.

To summarise the diverse literature reporting the impact of COVID-19 on health utility in COVID-19 patients as well as in general populations being affected by COVID-19 control policies. A literature search up to April 2023 was conducted to identify papers reporting health utility in COVID-19 patients or in COVID-19-affected general populations. We present a narrative synthesis of the health utility values/losses of the retained studies to show the mean health utility values/losses with 95% confidence intervals. Mean utility values/losses for categories defined by medical attendance and data collection time were calculated using random-effects models. In total, 98 studies-68 studies on COVID-19 patients and 30 studies on general populations-were retained for detailed review. Mean (95% CI) health utility values were 0.83 (0.81, 0.86), 0.78 (0.73, 0.83), 0.82 (0.78, 0.86) and 0.71 (0.65, 0.78) for general populations, non-hospitalised, hospitalised and ICU patients, respectively, irrespective of the data collection time. Mean utility losses in patients and general populations ranged from 0.03 to 0.34 and from 0.02 to 0.18, respectively. This scoping review provides a summary of the health utility impact of COVID-19 and COVID-19 control policies. COVID-19-affected populations were reported to have poor health utility, while a high degree of heterogeneity was observed across studies. Population- and/or country-specific health utility is recommended for use in future economic evaluation on COVID-19-related interventions.

CRISPR-Cas in Pseudomonas aeruginosa provides transient population-level immunity against high phage exposures.

The prokaryotic adaptive immune system, CRISPR-Cas (clustered regularly interspaced short palindromic repeats; CRISPR-associated), requires the acquisition of spacer sequences that target invading mobile genetic elements such as phages. Previous work has identified ecological variables that drive the evolution of CRISPR-based immunity of the model organism Pseudomonas aeruginosa PA14 against its phage DMS3vir, resulting in rapid phage extinction. However, it is unclear if and how stable such acquired immunity is within bacterial populations, and how this depends on the environment. Here, we examine the dynamics of CRISPR spacer acquisition and loss over a 30-day evolution experiment and identify conditions that tip the balance between long-term maintenance of immunity versus invasion of alternative resistance strategies that support phage persistence. Specifically, we find that both the initial phage dose and reinfection frequencies determine whether or not acquired CRISPR immunity is maintained in the long term, and whether or not phage can coexist with the bacteria. At the population genetics level, emergence and loss of CRISPR immunity are associated with high levels of spacer diversity that subsequently decline due to invasion of bacteria carrying pilus-associated mutations. Together, these results provide high resolution of the dynamics of CRISPR immunity acquisition and loss and demonstrate that the cumulative phage burden determines the effectiveness of CRISPR over ecologically relevant timeframes.

Weight Loss Diets, Fads, and Trends.

To review popular dietary trends and provide recommendations regarding validated dietary approaches for weight loss in the pediatric population. Like adults, children and adolescents trying to lose weight will succumb to diets promoted by the media. Many of these so-called "fad" diets tout unsupported claims for health but prove very difficult for long-term adherence. Since childhood is a pivotal time for establishing lifestyle habits, we need to provide practical dietary advice supported by scientific research. Studies suggest that emphasizing macronutrient balance while limiting both ultraprocessed foods and sugar-sweetened beverages can help our pediatric patients achieve and maintain a healthy weight. We review literature discouraging the use of restrictive dieting in the pediatric population and instead encourage a whole-foods-based, balanced dietary approach, along with regular physical activity. The goal is to support reasonable and sustainable lifestyle habits that ultimately allow children to establish lifelong health-promoting behaviors.

Oral Health Literacy and Tooth Loss and Replacement in Older Adults at a University Dental Clinic in Colombia.

Oral health literacy (OHL) is the ability of individuals to obtain, process, and understand oral health information and services, allowing them to make appropriate oral health decisions. The association between OHL and tooth loss and replacement have not been well understood. We aimed to determine the association between OHL and tooth loss and replacement in a Colombia population. A cross-sectional study of 384 older adults age 65 to 89 years from Pasto, Colombia was carried out. The number of lost and replaced teeth was assessed intraorally; sociodemographic and prosthetic characteristics were collected, and the Health Literacy in Dentistry questionnaire was used to evaluate OHL. Generalized linear models were estimated to assess associations between independent variables (including OHL) and the number of lost and replaced teeth. There were 224 (58.3%) men and 160 (41.7%) women. The mean (standard deviation [SD]) number of lost and replaced teeth was 27.78 (4.03) and 12.53 (9.89), respectively. One hundred fifty five (40.4%) individuals had full removable dental protheses, 122 (31.8%) partial removable dental protheses, 68 (17.7%) fixed prosthetics, and 36 (9.4%) dental implants. OHL was 33.29 (6.59) and significantly positively associated with the number of replaced teeth (β = 0.65, 95% confidence interval [CI]: 0.52-0.78, p < .001), but not with lost teeth. OHL may foster individuals' capabilities to replace lost teeth, although we did not find it associated with reduced tooth loss, likely as tooth loss was highly common in this older population. [HLRP: Health Literacy Research and Practice. 2024;8(1):e21-e28.].

Mid-Infrared Photons Alleviate Tinnitus by Activating the KCNQ2 Channel in the Auditory Cortex.

Tinnitus is a phantom auditory sensation often accompanied by hearing loss, cognitive impairments, and psychological disturbances in various populations. Dysfunction of KCNQ2 and KCNQ3 channels-voltage-dependent potassium ion channels-in the cochlear nucleus can cause tinnitus. Despite the recognized significance of KCNQ2 and KCNQ3 channels in the auditory cortex, their precise relationship and implications in the pathogenesis of tinnitus remain areas of scientific inquiry. This study aimed to elucidate the pathological roles of KCNQ2 and KCNQ3 channels within the auditory cortex in tinnitus development and examine the therapeutic potential of mid-infrared photons for tinnitus treatment. We utilized a noise-induced tinnitus model combined with immunofluorescence, electrophysiological recording, and molecular dynamic simulation to investigate the morphological and physiological alterations after inducing tinnitus. Moreover, invivo irradiation was administered to verify the treatment effects of infrared photons. Tinnitus was verified by deficits of the gap ratio with similar prepulse inhibition ratio and auditory brainstem response threshold. We observed an important enhancement in neuronal excitability in the auditory cortex using patch-clamp recordings, which correlated with KCNQ2 and KCNQ3 channel dysfunction. After irradiation with infrared photons, excitatory neuron firing was inhibited owing to increased KCNQ2 current resulting from structural alterations in the filter region. Meanwhile, deficits of the acoustic startle response in tinnitus animals were alleviated by infrared photons. Furthermore, infrared photons reversed the abnormal hyperexcitability of excitatory neurons in the tinnitus group. This study provided a novel method for modulating neuron excitability in the auditory cortex using KCNQ2 channels through a nonthermal effect. Infrared photons effectively mitigated tinnitus-related behaviors by suppressing abnormal neural excitability, potentially laying the groundwork for innovative therapeutic approaches for tinnitus treatment.

Artificial intelligence-aiding lab-on-a-chip workforce designed oral [3.1.0] bi and [4.2.0] tricyclic catalytic interceptors inhibiting multiple SARS-CoV-2 protomers assisted by double-shell deep learning.

While each massive pandemic has claimed the lives of millions of vulnerable populations over the centuries, one limitation exists: that the Edisonian approach (human-directed with trial errors) relies on repurposing pharmaceuticals, designing drugs, and herbal remedies with the violation of Lipinski's rule of five druglikeness. It may lead to adverse health effects with long-term health multimorbidity. Nevertheless, declining birth rates and aging populations will likely cause a shift in society due to a shortage of a scientific workforce to defend against the next pandemic incursion. The challenge of combating the ongoing post-COVID-19 pandemic has been exacerbated by the lack of gold standard drugs to deactivate multiple SARS-CoV-2 protein targets. Meanwhile, there are three FDA-approved antivirals, Remdesivir, Molnupiravir, and Paxlovid, with moderate clinical efficacy and drug resistance. There is a pressing need for additional antivirals and prepared omics technology to combat the current and future devastating coronavirus pandemics. While there is a limitation of existing contemporary inhibitors to deactivate viral RNA replication with minimal rotational bonds, one strategy is to create Lipinski inhibitors with less than 10 rotational bonds and precise halogen bond placement to destabilize multiple viral protomers. This work describes the efforts to design gold-standard oral inhibitors of bi- and tri-cyclic catalytic interceptors with electrophilic heads using double-shell deep learning. Here, KS1 with and KS2 compounds designed by lab-on-a-chip technology attain 5-fold novel filtered-Lipinski, GHOSE, VEBER, EGAN, and MUEGGE druglikeness. The graph neural network (GNN) relies on module-initiation, expansion, relabeling atom index, and termination (METORITE) iterations, while the deep neural network (DNN) engages pinning, extraction, convolution, pooling, and flattening (PROOF) operations. The cyclic compound's specific halogen atom location enhances the nitrile catalytic head, which deactivates several viral protein targets. Initiating this lab-on-a-chip that is not susceptible to the aging process for creating clinical compounds can leverage a new path to many valuable drugs with speedy oral drug discovery, especially to defend the loss of vulnerable population and prevent multimorbidity that is susceptible to hidden viral persistence in the continuing aging times.

Comprehensive aesthetic assessment and optimization of green spaces in the landscape and decorative park of the Stavropol Botanical Garden

The work examines the stages of creation and the current state of the landscape and decorative park of the Stavropol Botanical Garden. The species composition, sanitary and aesthetic condition of growing plants was analyzed. A comprehensive assessment of the recreational potential of the territory is given, with the definition of the class of recreational value. The reasons for the deterioration of the condition and aesthetic qualities of plantings have been identified. Recommendations have been formed to prevent the destruction and loss of plant populations, as well as to improve their architectural, landscape and functional efficiency.