Polyphosphate-accumulating Organisms Research Articles

Ultra-high nitrogen removal from real municipal wastewater using selective enhancement of glycogen accumulating organisms (GAOs) in a partial nitrification-anammox (PNA) system

Integrating endogenous denitrification (ED) into partial nitrification-anammox (PNA) systems by adequately utilizing organics in municipal wastewater is a promising approach to improve nitrogen removal efficiency (NRE). In this study, a novel strategy to inhibit phosphorus-accumulating organisms (PAOs) by inducing phosphorus release and exclusion was adopted intermittently, optimizing organics allocation between PAOs and glycogen-accumulating organisms (GAOs). Enhanced ED-synergized anammox was established to treat real municipal wastewater, achieving an NRE of 97.5±2.2% and effluent total inorganic nitrogen (TIN) of less than 2.0 mg/L. With low poly-phosphorus (poly-P) levels (poly-P/VSS below 0.01 (w/w)), glycogen accumulating metabolism (GAM) acquired organics exceeded that of phosphorus accumulating metabolism (PAM) and dominated endogenous metabolism. Ca. Competibacter (GAO) dominated the community following phosphorus-rich supernatant exclusion, with abundance increasing from 3.4% to 5.7%, accompanied by enhanced ED capacity (0.2 to 1.4 mg N/g VSS /h). The enriched subgroups (GB4, GB5) of Ca. Competibcater established a consistent nitrate cycle with anammox bacteria (AnAOB) through endogenous partial denitrification (EPD) at a ∆NO2−-N/∆NH4+-N of 0.91±0.11, guaranteeing the maintenance of AnAOB abundance and performance. These results provide new insights into the flexibility of PNA for the energy-efficient treatment of low-strength ammonium wastewater.

Impact of operational strategies on a sidestream enhanced biological phosphorus removal (S2EBPR) reactor in a carbon limited wastewater plant.

Water resource recovery facilities are faced with stringent effluent phosphorus limits to reduce nutrient pollution. Enhanced biological phosphorus removal (EBPR) is the most common biological route to remove phosphorus; however, many facilities struggle to achieve consistent performance due to limited carbon availability in the influent wastewater. A promising process to improve carbon availability is through return activated sludge (RAS) fermentation via sidestream EBPR (S2EBPR). In this study, a full-scale S2EBPR pilot was operated with a sidestream plus carbon configuration (SSRC) at a carbon-limited facility. A model based on the pilot test was developed and calibrated in the SUMO platform and used to explore routes for improving orthophosphate (OP) effluent compliance. Modeling results showed that RAS diversion by itself was not sufficient to drive OP removal to permit limits of 1 mg L-1, therefore, other strategies were evaluated. Supplemental carbon addition of MicroC® at 1.90 L min-1 and controlling the phosphorus concentration below 3.5 mgP L-1 in the primary effluent (PE) proved to be valid supplemental strategies to achieve OP removal below 1 mg L-1 most of the time. In particular, the proposed supplemental carbon flow rate would result in an improvement of the rbCOD:P ratio from 17:1 to 26:1. The synergistic approach of RAS diversion and supplemental carbon addition increased the polyphosphate accumulating organisms (PAO) population while minimizing the supplemental carbon needed to achieve consistent phosphorus removal. Overall, this pilot and modeling study shows that joint strategies, including RAS diversion, carbon addition and PE control, can be effective to achieve optimal control of OP effluent.

Advanced nutrient removal and external sludge reduction: Demonstration in a pilot-scale sequencing batch reactor.

Although the addition of excess sludge fermentation products to improve nutrient removal from sewage is cost-effective, its application has rarely been demonstrated. In this study, the external sludge was first collected and fermented under a sludge retention time of 10days, then introduced into SBR with a 1:15 sewage ratio. The results revealed a gradual increase in the nitrite accumulation ratio to 34.7% in the SBR at the end of the oxic stage after 64 days of adding fermented sludge products. In addition, the average effluent total nitrogen and phosphorous decreased to 7.3 and 0.5mg/L, corresponding to removal efficiencies of 86.7% and 95.5%, respectively. On the other hand, the use of the fermented sludge products as external organic carbon sources in the SBR increased the external sludge reduction ratio to 42.5%. High-throughput sequencing demonstrated that the increase in the endogenous denitrifier community, polyphosphate-accumulating organisms, and fermentation bacteria were the main factors contributing to the increase in nutrient removal and excess sludge reduction. The economic evaluation indicated that the operational cost of the pilot-scale system saves 0.011$/m3 of sewage treated. PRACTITIONER POINTS: Fermented sludge addition effectively enhanced nutrient removal in pilot-scale SBR. Average effluent TN and PO4 3- -P decreased to 7.3 and 0.5 mg/L, respectively. Highest external sludge reduction rate was 42.5% in pilot-scale reactor. Sewage treatment cost can save 0.011$/m3 under advanced nutrient removal.

Ecological risk assessment of glyphosate and its possible effect on bacterial community in surface sediments of a typical shallow Lake, northern China

Glyphosate is a widely used herbicide worldwide and its prevalent presence in aquatic ecosystems poses a threat to living organisms. This study evaluated potential ecological risk of glyphosate to sediment-dwelling organisms and assessed the probable effect of glyphosate on structure and predicated function of sediment-attached bacterial communities from a large shallow lake in northern China based on 16S rRNA high-throughput sequencing. Results suggested that glyphosate showed a medium to high concentration (up to 8.63 mg/kg) and chronic risk to sediment-dwelling organisms (10% samples exhibiting medium to high risk quotient), especially in sites nearby farmland and residential areas in August. Bacterial community identification based on 16S rRNA sequence indicated some species of dominant phylum Proteobacteria and Campilobacterota (e.g., Steroidobacteraceae, Thiobacillus, Gallionellaceae, Sulfurimonadaceae) were stimulated while some species of dominant phylum Actinobacteriota, Acidobacteriota and Firmicutes (e.g., Nocardioidaceae, Microtrichales, Vicinamibacteraceae, Paenisporosarcina) were inhibited by glyphosate accumulation. The stimulating species were related to sulfur-oxidizing, sulfate-, iron-, or nitrate-reducing bacteria; The inhibiting species were related to plant bacterial endophytes, polyphosphate-accumulating organisms (PAOs) and denitrifers. Correspondingly, promoted bacterial metabolic functions of “sulfite respiration”, “nitrogen respiration”, “aromatic compound degradation” and “nitrification” but suppressed “cellulolysis”, “manganese oxidation”, “anoxygenic photoautotrophy S oxidizing” and “nitrate denitrification” were predicated on functional annotation of prokaryotic taxa. Although these results could only partly suggest the impacts of glyphosate on the bacterial communities due to the lack of actual results from control experiments, the identified Steroidobacteraceae could be thought as a bioindicator in the future mechanism study for the ecological effect and bioremediation of glyphosate. This work intends to arise the concern about the depletion of biodiversity and bacterial metabolic functions with contribution of glyphosate in part in eutrophic lakes.

Exploring the stability of an A-stage-EBPR system for simultaneous biological removal of organic matter and phosphorus

This work evaluates the performance and stability of a continuous anaerobic/aerobic A-stage system with integrated enhanced biological phosphorus removal (A-stage-EBPR) under different operational conditions. Dissolved oxygen (DO) in the aerobic reactor was tested in the 0.2–2 mgDO/L range using real wastewater amended with propionic acid, obtaining almost full simultaneous COD and P removal without nitrification in the range 0.5–1 mgDO/L, but failing at 0.2 mgDO/L. Anaerobic purge was tested to evaluate a possible mainstream P-recovery strategy, generating a P-enriched stream containing 22% of influent P. COD and N mass balances indicated that about 43% of the influent COD could be redirected to the anaerobic digestion for methane production and 66% of influent NH4+-N was discharged in the effluent for the following N-removal B-stage. Finally, when the system was switched to glutamate as sole carbon source, successful EBPR activity and COD removal were maintained for two months, but after this period settleability problems appeared with biomass loss. Microbial community analysis indicated that Propionivibrio, Thiothrix and Lewinella were the most abundant species when propionic acid was the carbon source and Propionivibrio was the most favoured with glutamate. Thiothrix, Hydrogenophaga, Dechloromonas and Desulfobacter appeared as the dominant polyphosphate-accumulating organisms (PAOs) under different operation stages.

Advanced nitrogen and phosphorus removal by the symbiosis of PAOs, DPAOs and DGAOs in a pilot-scale A2O/A+MBR process with a low C/N ratio of influent

Cooperating in harmony to avoid competition with dominant functional microbial symbiosis is an efficient way in advanced nitrogen and phosphorus removal in wastewater treatment processes. In this study, a niche-based coordinating strategy was implemented to cooperate in harmony with phosphorus-accumulating organisms (PAOs), denitrifying phosphorus-accumulating organisms (DPAOs) and denitrifying glycogen-accumulating organisms (DGAOs) to advance nitrogen and phosphorus removal based on an anaerobic-anoxic-oxic-anoxic-membrane bioreactor (A2O/A+MBR) under low C/N in municipal wastewater influent. The niche-based strategy was conducted based on the ORP change during the process as an indicator combined with the adjustment of recirculation and anoxic zone shifting. The results indicated that the strategy of the post-anoxic unit could enable significant enhancement of biological nitrogen and phosphorus removal (BNPR) by 9.9% and 16.3%, respectively, with low effluent concentrations of 7.0 ± 2.2 mg N/L and 0.36±0.32 mg P/L. The satisfactory performance was dominated along with the shift in the microbial community: the relative abundance of Tetrasphaera (PAO genus) increased from 0.14±0.08% to 0.32±0.12%, while the relative abundance of Decchloromonas (DGAO genus) and Candidatus Competibacter (DGAO genus) also increased. The advanced combination of anaerobic phosphorus release, anoxic denitrification, denitrifying phosphorus removal and endogenous denitrification was qualified by the modeling simulation of the biochemical kinetics mechanism of activated sludge in the A2O+MBR and A2O/A+MBR processes, which means that cooperation in the harmony of PAOs, DPAOs and DGAOs could be efficiently realized by a promising control strategy to enhance BNPR in an A2O+MBR with a post-anoxic unit. This study provides an efficient and simple novel control strategy to overcome the limitation of traditional nitrogen and phosphorus removal under an insufficient carbon source.

Balancing denitrifying phosphorus-accumulating organisms and denitrifying glycogen-accumulating organisms for advanced nitrogen and phosphorus removal from municipal wastewater

Given the carbon limitation of municipal wastewater, the balance of biological nitrogen and phosphorus removal remains a challenging task. In this study, an anaerobic-anoxic–oxic combining with biological contact oxidation (A2/O-BCO) system treating real municipal wastewater was operated for 205 days, and COD-to-PO43--P ratio was confirmed as the key parameter for balancing denitrifying phosphorus-accumulating organisms (DPAOs) and denitrifying glycogen-accumulating organisms (DGAOs) to enhance N and P removal. When DPAOs dominated in nutrients removal, the increase in COD/P from 17.1 to 38.1 caused the deterioration in nitrogen removal performance decreasing to 71.8 %. As COD/P ratio decreased from 81.3 to 46.8, Ca.Competibacter proliferated from 3.11 % to 6.00 %, contributing to 58.9 % of nitrogen removal. The nitrogen and phosphorus removal efficiency reached up to 79.3 % and 95.2 %. Overall, establishing DGAOs-DPAOs balance by strengthening the effect of DGAOs could enhance the nutrients removal performance and accordingly improve the stability and efficiency of the system.

Predicting the impact of temperature on metabolic fluxes using resource allocation modelling: Application to polyphosphate accumulating organisms

The understanding of microbial communities and the biological regulation of its members is crucial for implementation of novel technologies using microbial ecology. One poorly understood metabolic principle of microbial communities is resource allocation and biosynthesis. Resource allocation theory in polyphosphate accumulating organisms (PAOs) is limited as a result of their slow imposed growth rate (typical sludge retention times of at least 4 days) and limitations to quantify changes in biomass components over a 6 hours cycle (less than 10% of their growth). As a result, there is no direct evidence supporting that biosynthesis is an exclusive aerobic process in PAOs that alternate continuously between anaerobic and aerobic phases. Here, we apply resource allocation metabolic flux analysis to study the optimal phenotype of PAOs over a temperature range of 4 °C to 20 °C. The model applied in this research allowed to identify optimal metabolic strategies in a core metabolic model with limited constraints based on biological principles. The addition of a constraint limiting biomass synthesis to be an exclusive aerobic process changed the metabolic behaviour and improved the predictability of the model over the studied temperature range by closing the gap between prediction and experimental findings. The results validate the assumption of limited anaerobic biosynthesis in PAOs, specifically “Candidatus Accumulibacter” related species. Interestingly, the predicted growth yield was lower, suggesting that there are mechanistic barriers for anaerobic growth not yet understood nor reflected in the current models of PAOs. Moreover, we identified strategies of resource allocation applied by PAOs at different temperatures as a result of the decreased catalytic efficiencies of their biochemical reactions. Understanding resource allocation is paramount in the study of PAOs and their currently unknown complex metabolic regulation, and metabolic modelling based on biological first principles provides a useful tool to develop a mechanistic understanding.

Positive effects of magnetic Fe3O4@polyaniline on aerobic granular sludge: Aerobic granulation, granule stability and pollutants removal performance

The magnetic material has been determined to have a positive effect on sludge granulation and wastewater treatment performance. In this study, the effect of magnetic Fe3O4@polyaniline (Fe3O4@PANI) on aerobic granulation, granule stability, and pollutants removal performance was evaluated by adding it into a sequencing batch reactor to cultivate aerobic granular sludge (AGS). The results indicated that the composite combined the advantages of PANI and Fe3O4 to promote the formation of AGS during the granulation period. The Fe3O4@PANI stimulated the granules to secrete extracellular polymeric substances with a higher proteins/polysaccharides ratio, thus enhancing the stability of the AGS. In addition, microbial community analysis revealed that the great performance of the AGS on denitrification and phosphorus removal could be attributed to the enrichment of denitrifying bacteria, phosphorus accumulating organisms (PAO), and denitrifying PAO by Fe3O4@PANI. Thus, Fe3O4@PANI has been demonstrated to have a positive effect on the formation and stability of AGS.

Glycerol conversion by aerobic granular sludge

Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date. The current study describes glycerol utilization by aerobic granular sludge and enhanced biological phosphorus removal (EBPR). Robust granules with good phosphorus removal capabilities were formed in an aerobic granular sludge sequencing batch reactor fed with glycerol. The interaction between the fermentative conversion of glycerol and product uptake by polyphosphate accumulating organisms (PAO) was studied using stoichiometric and microbial community analysis. Metagenomic, metaproteomic and microscopic analysis identified a community dominated by Actinobacteria (Tessaracoccus and Micropruina) and a typical PAO known as Ca. Accumulibacter. Glycerol uptake facilitator (glpF) and glycerol kinase (glpK), two proteins involved in the transport of glycerol into the cellular metabolism, were only observed in the genome of the Actinobacteria. The anaerobic conversion appeared to be a combination of a substrate fermentation and product uptake-type reaction. Initially, glycerol fermentation led mainly to the production of 1,3-propanediol (1,3-PDO) which was not taken up under anaerobic conditions. Despite the aerobic conversion of 1,3-PDO stable granulation was observed. Over time, 1,3-PDO production decreased and complete anaerobic COD uptake was observed. The results demonstrate that glycerol-containing wastewater can effectively be treated by the aerobic granular sludge process and that fermentative and polyphosphate accumulating organisms can form a food chain in glycerol-based EBPR processes.

Low-temperature-resistance granulation of activated sludge and the microbial responses to the granular structural stabilization

Completely loss of granular structural stability and reliable start-up of aerobic granular sludge (AGS) system are considered as the biggest challenges for its engineering application under seasonal temperature variation, especially extremely low temperatures. In this study, two identical sequencing batch reactors (SBR) were successfully start-up at 10 °C (R1) and 25 °C (R2), respectively, and then operated under a strategy of stepwise change of temperatures to investigate the stability of the granular sludge by examining its microbial characteristics, bis (3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP), extracellular polymeric substance (EPS) and sludge physiochemical properties. The results showed that AGS formed under the low temperature preferentially secrete EPS and c-di-GMP for stable granulation and improvement of its resistance to temperature changes. Meanwhile, R1 successfully obtained aerobic granulation with high biomass concentration and superior settleability, as well as high pollutant removal performance. In comparison, R2 took a longer time for granulation and was subjected to serious disintegration of AGS. The matrix structure partially formed by filamentous bacteria during the start-up stage in R1 was one of major reasons for its own superiority beyond R2 in granulation. Slow-growing organisms such as autotrophic nitrifying and Anammox bacteria, phosphorus accumulation organisms, EPS-producing genera, and c-di-GMP pathway-dependent genera, were exclusively enriched in the R1 and resulted in higher pollutants removal efficiencies and stable structure, whereas Sphaerotilus dominated in R2 that related closely with its unstable performance. Therefore, the strategy based on the stepwise change of temperatures from extremely low temperatures may be one feasible way for the sustainable application of AGS system, which is of significance to address the challenging problems of AGS applications.

The Occurrence and Role of Tetrasphaera in Enhanced Biological Phosphorus Removal Systems

The application of enhanced biological phosphorus removal (EBPR) in wastewater treatment plants (WWTPs) has commonly been utilized worldwide. However, the optimum efficiency has not been realized over the past decades, prompting many studies and publications. The limitations, especially comprehension of the abundance and actual potential of polyphosphate-accumulating organisms (PAOs), are not fully understood. Recently identified putative PAOs, Tetrasphaera, present a vast metabolic versatility compared to Candidatus Accumulibacter. The characterisation of Tetrasphaera unique abilities to utilize various carbon substrates, volatile fatty acids production and consistent high abundance, presents potential boosts towards the process efficiency improvement. This paper provides the existing knowledge on the physiology, morphology and genetic description of PAOs with a special attention to the current state of research on Tetrasphaera and its potential. In addition, process conditions and their influence on the microbial activities in EBPR systems are discussed.

Biomineralization and AHLs-guided quorum sensing enhanced phosphorus recovery in the alternating aerobic/anaerobic biofilm system under metal ion stress

The alternating aerobic/anaerobic biofilm system had been applied for phosphorus (P) enrichment and recovery because of the advantage of low energy consumption and high efficiency. The metal ions and N-acyl-L-homoserine lactones (AHLs) in system were studied to better clarify the mechanism of P uptake/release under metal ion stress. The results indicated that the increase of metal ions stimulated the release of AHLs, and AHLs-guided quorum sensing (QS) enhanced P uptake. Moreover, biomineralization could stimulate the increase of P content in biofilm (Pbiofilm). Meanwhile, some ortho-p was converted to short-chain poly-p in extracellular polymer substance (EPS), and others were transferred into cell through EPS to synthesize poly-p. With the Pbiofilm increased, more P could be absorbed/released due to the shift in the metabolic model of polyphosphate accumulating organisms (PAOs). The release of AHLs between microorganisms was also inhibited when PAOs reached the state of P saturation (75.6 ± 2.5 mg/g SS), which meant that the effect of signaling function would tend to stabilize, and the 169.2 ± 2.6 mg/L P concentration in the enriched solution was obtained due to the P release was inhibited. Moreover, P was rapidly transferred to the new enriched solution after the P was recovered, and PAOs restored its capability of P uptake/release. In addition, 31P-NMR analysis demonstrated that EPS played a major role in PAOs compared to cell, and inorganic phosphorus (IP) played an essential role in the uptake/release of P compared to organic phosphorus (OP). Furthermore, the microbiological analysis showed that Candidatus Accumulibacter was positively correlated with AHLs (P < 0.05). This study provided essential support for clarifying the P metabolism mechanism of PAOs.

Recovering phosphorus as vivianite using an alternating aerobic/anaerobic biofilm and fluidized bed crystallization coupled system

An alternating aerobic/anaerobic biofilm (AABF) and fluidized bed crystallization (FBC) coupled system was constructed to examine the feasibility of using phosphorus (P) metabolic mechanism to enrich P and the availability of large size and high-purity vivianite crystals in this study. The results demonstrated that the stable operation of AABF system could be maintained by operating the biofilm system through P metabolic mechanism of polyphosphate accumulating organisms (PAOs), and the P concentration of 204.5 ± 3.2 mg/L could be obtained. The metabolic mode of PAOs shifted from GAM to PAM as the Pbiofilm increased, which led to PAOs being able to absorb/release more P, wherein extracellular polymer substance (EPS) and cell both played an indispensable role in the metabolism of P uptake/release. In the P recovery stage, the maximum P recovery efficiency reached 94.6 ± 3.1 %, and the main crystals formed were vivianite crystals. Moreover, the percentage of FeP and CaP was 80–93 % and 3–9 % in precipitate, and the percentage of phosphate used to synthesize vivianite was 83.2 %. Furthermore, the size of vivianite crystal increased with the generated vivianite as seed crystal. What's more, the LCA revealed that carbon dioxide equivalent (CO2 eq.) emissions, sulfur dioxide equivalent (SO2 eq.) emissions and phosphate equivalent (PO4− eq.) emissions were 13.3–15.1 kg CO2 eq./kg vivianite, 0.10–0.11 kg SO2 eq./kg vivianite, 0.031–0.037 kg PO4− eq./kg vivianite, respectively. The pH = 7 and Fe: P = 1.5:1 was the optimum condition to ensure high P recovery efficiency and reduced potential environmental emissions. This process system laid the groundwork for recovering P from wastewater.

Mathematical modeling of the dynamic effect of denitrifying glycogen-accumulating organisms on nitrous oxide production during denitrifying phosphorus removal

The large amount of intermediate nitrous oxide (N2O) production from denitrifying phosphorus removal (DPR) increases the carbon footprint of wastewater treatment. However, there is a lack of detailed understanding of the interrelationships between denitrifying polyphosphate-accumulating organisms (DPAOs) and denitrifying glycogen-accumulating organisms (DGAOs) on N2O production during DPR. In this work, a mathematical model was developed for the first time to describe dynamic N2O production in the DPR system coexisting DPAOs and DGAOs. The model took into account a four-step subsequent denitrification of nitrate, nitrite, nitric oxide, and N2O. The validity of model was fully tested by comparing simulation studies with experimental data from three independent reports on DPR, which satisfactorily described the dynamics of N2O production, nitrogen oxide reduction, phosphate release and uptake, and intracellular polymers turnover. The validated model could clarify the source and pathways of N2O production. Subsequently, the combined effects of key operational conditions on the overall N2O production, DPAOs and DGAOs competition, and nutrients removal efficiency were investigated by model simulation. Simulation results showed higher polyhydroxyalkanoate storage rate of DGAOs than that of DPAOs during anaerobic stage and the preference of DGAOs for nitrate electron acceptor during anoxic stage. DGAOs dominated the growth competition over DPAOs at low COD (<150 mg/L) and high nitrate (>35 mg/l) conditions, leading to the anoxic storage of glycogen by DGAOs as the main pathway of N2O production. In addition, both N2O production and generation pathways in the system possessed greater variability compared to single DGAOs or DPAOs system, further indicating the necessity of this model.

Step-feeding in aerobic/anoxic cycles enhanced the performance of aerobic granular sludge (AGS) systems treating effluents with low C:N ratios

Aerobic granular sludge (AGS) systems treating effluents with a C:N:P ratio similar to real old landfill leachate were evaluated on simultaneous C, N and P removals, and to reduce the main problems encountered, such as nitrite accumulation, biomass loss, and granule disintegration. Therefore, six sequential batch reactors (SBR) were operated with different anaerobic (A), anoxic (An), and aerobic (O) configurations: A/O (R1 and R2), O/An with conventional feeding and well-defined anoxic phase (R3), O/An with step-feeding and well-defined anoxic phase (R4), and O/An (R5 and R6). The O/An with step-feeding reactor (R4) had the highest biomass retention/settleability (SVI30 < 50 mL/g), the best nitrification rates (99%), and chemical oxygen demand (COD) (97%), total nitrogen (91%) and total phosphorous (55%) removals. Furthermore, there was no nitrite accumulation, and granules’ disintegration was insignificant. The most abundant phylum in the reactors O/An was Planctomycetota, composed mainly of organisms from the Pirellulaceae and Legionellaceae families. In these reactors, the abundance of phosphorus-accumulating organisms (PAOs) and denitrifying bacteria were similar, while the abundance of glycogen-accumulating organisms was much higher than PAOs. Therefore, the type of cycle directly influences performance, granule characteristics, and system stability, being important for future investigations applying the AGS technology to leachate treatment.

Modelling the impacts of operational conditions on the performance of a full-scale membrane aerated biofilm reactor

Membrane Aerated Biofilm Reactors (MABR) are gaining more and more acceptance in the plethora of wastewater process intensification technologies. Mathematical modelling has contributed to show their feasibility in terms of reduced energy consumption and footprint. Nevertheless, most simulation studies published until now are still focused on analyzing MABR as single units and not fully integrated within the flow diagram of the water treatment plant (WWTP). In this paper, the prediction capabilities of an integrated modelling approach is tested using full-scale data from Ejby Mølle WWTP+MABR site (Odense, Denmark). Mass balances, data reconciliation methods, process simulation and the different evaluation criteria were used to adjust influent, effluent and process indicators. Results show 10 % mismatch between flow, COD, N and P predictions and measurements in different plant locations. Using the adopted hydraulic retention time (HRT), nitrogen load (NL), membrane surface area (MA) and oxygen transfer rate (OTR), it was possible to predict nitrification rates (NR) within the interquartile range. This has been done under two different MABR operational conditions: with (#S2) and without (#S1) external aeration (EA) in the bulk liquid. The model provides additional process insights about biofilm structure, substrate gradients, weak acid base chemistry and precipitation potential. More specifically, simulations suggest the potential undesirable effects of sulfate (SRB) and iron reducing bacteria (IRB) on both microbial activity and composition of the biofilm. The latter may have a strong impact on ammonium (NHx), sulfate (SOx) and ferrous ion (Fe+2) conversion processes. The change of operational strategy in the scenario analysis highlights that the denitrifying activity of phosphorus accumulating organisms (PAOs) can enhance nutrient removal in MABR tanks. In addition, it was possible to assess the chance of success (in terms of energetic cost of nitrogen removal) of adding several MABR units in one tank of the WWTP under study before full-scale implementation.

Enhancing resource recovery via cranberry syrup waste at the Wisconsin Rapids WRRF: An experimental and modeling study

The Wisconsin Rapids Wastewater Treatment Plant (WRWWTP) is faced with a more stringent effluent phosphorus requirement that will drive capital investment between 2020 and 2025. The facility will need to achieve a monthly average value of 0.36 mg L−1 of total phosphorus (TP). While the facility has sufficient influent carbon to drive a conventional enhanced biological phosphorus removal (EBPR) configuration, the existing infrastructure makes the addition of influent selector zones cost prohibitive. Underutilized aeration basin capacity was repurposed for testing return activated sludge (RAS) fermentation. The WRWWTP began pilot testing of RAS fermentation in April 2021. The facility moved through a series of operational setpoints to optimize phosphorus removal in a sidestream RAS (SSR) configuration, including RAS diversion, decrease of DO in aeration basins and chemical dosing shutoff. One of the key implementations was the addition of cranberry syrup waste to provide additional carbon for RAS fermentation, converting the process to a SSR plus carbon (SSRC) configuration. By the end of the testing period, effluent total phosphorus was averaging less than 0.4 mg L−1 with no chemical addition. A model was developed in the SUMO platform and was used to capture orthophosphate trends during the testing period. The model investigated microbial population dynamics and found that the operational changes including RAS diversion, chemical dosing shutoff and cranberry syrup waste addition impacted the enrichment of phosphorus accumulating organisms (PAO). After performing a sensitivity analysis on hydrolysis parameters, the predicted hydrolysis rate around 1.8–1.9 mg COD g VSS−1 hr−1 was found to match the batch rate testing data. This is the first study where cranberry syrup waste was used to successfully enhance EBPR performance, resulting in 90% TP removal. While further research is needed regarding the composition of the waste matrix and the microbial community composition, this expands the routes for resource recovery in the field of wastewater treatment.

Comparing with oxygen, nitrate simplifies microbial community assembly and improves function as an electron acceptor in wastewater treatment

Biochemical oxidation and reduction are key processes in treating biological wastewater and they require the presence of electron acceptors. The functional impact of electron acceptors on microbiomes provides strategies for improving the treatment efficiency. This research focused on two of the most important electron acceptors, nitrate and oxygen. Molecule ecological network, null model, and functional prediction based on high-throughput sequencing were used to analyze the microbiomes features and assembly mechanism. The results revealed nitrate via the homogeneous selection (74.0%) decreased species diversity, while oxygen via the homogeneous selection (51.1%) and dispersal limitation (29.6%) increased the complexity of community structure. Microbes that were more strongly homogeneously selected for assembly included polyphosphate accumulating organisms (PAOs), such as Pseudomonas and variovorax in the nitrate impacted community; Pseudomonas, Candidatus_Accumulibacter, Thermomonas and Dechloromonas, in the oxygen impacted community. Nitrate simplified species interaction and increased the abundance of functional genes involving in tricarboxylic acid cycle (TCA cycle), electron transfer, nitrogen metabolism, and membrane transport. These findings contribute to our knowledge of assembly process and interactions among microorganisms and lay a theoretical basis for future microbial regulation strategies in wastewater treatment.

Impacts of dibutyl phthalate on biological municipal wastewater treatment in a pilot-scale A2/O-MBR system

Dibutyl phthalate (DBP) is a typical contaminant in pharmaceutical wastewater with strong bio-depressive properties which potentially affects the operation of municipal wastewater treatment systems. Based on a year-round monitoring of the quality of influent and effluent of a full-scale pharmaceutical wastewater treatment plant in Northeast China, the DBP was found to be the representative pollutant and its concentration in the effluent ranged 4.28 ± 0.93 mg/L. In this study, the negative effects of DBP on a pilot-scale A2/O-MBR system was investigated. When the influent DBP concentration reached 8.0 mg/L, the removals of chemical oxygen demand (COD) and total nitrogen (TN) were significantly inhabited (P < 0.01), with the effluent concentration of 54.7 ± 2.6 mg/L and 22.8 ± 3.7 mg/L, respectively. The analysis of pollutant removal characteristics of each process unit showed that DBP had the most significant effects on the removals of COD and TN in the anoxic tank. The α- and β-diversity in the system decreased significantly when the influent DBP concentration reached 8.0 mg/L. The impacts of DBP on known nitrifying bacteria, such as Nitrospira, and phosphorus accumulating organisms (PAOs), such as Cadidatus Accumulibacter, were not remarkable. Whereas, DBP negatively affected the proliferation of key denitrifying bacteria, represented by Simplicispira, Dechloromonas and Acinetobacter. This study systematically revealed the impacts of DBP on the pollutants removal performance and the bacterial community structure of the biological municipal wastewater treatment process, which would provide insights for understanding the potential impacts of residues in treated pharmaceutical wastewater on biological municipal wastewater treatment.