Polymorphic Method Research Articles

Polymorphic Distributed Energy Management for Low-Carbon Port Microgrid With Carbon Capture and Carbon Storage Devices

In order to reduce the carbon emission of the port and build a green port, a polymorphic distributed energy management method for the low carbon port microgrid with carbon capture and carbon storage device is proposed. Firstly, this paper presents a low carbon port microgrid in a polymorphic network environment to realize the information interaction among energy subjects in different modes and improve network communication performance among port power generation device, main grid, carbon capture and carbon storage device. Secondly, the energy management model of low-carbon port microgrid is constructed considering the additional carbon capture device and carbon storage device in the port. Then, based on the multi-agent consensus algorithm, a distributed energy management method is proposed, which is respectively oriented to the grid-connected operation mode, island operation mode and switching operation mode of the port microgrid, so as to achieve the economic, low carbon and reliable operation of the port microgrid. Finally, the simulation results of Matlab verify the effectiveness of the proposed method.

Combining AI Techniques for Recognizing Aerobics Sport Videos.

In order to improve the recognition accuracy of aerobics athletes' action features, a multifeature fusion-supported aerobics footprint recognition framework was proposed in this work. By extracting and constructing the 3D peripheral structure reconstruction scheme of the concept of aerobics footprint, we combine the fuzzy feature decomposition method to decompose the aerobics footprint image with multi-pane and suggest fusion. We further realize the similarity identification of aerobics athlete's trajectory through the wear polymorphic liquefaction method. Simulation results have shown that our proposed method has better effect on the similarity recognition of bodybuilders' footprints, a higher accuracy of behavior location, a satisfactory notification time, and an accurate recognition of bodybuilders' footprints. Experiments have shown that our designed system has a small mean square error of aerobics movements and a confirmation of absolute failure. It also has a high recognition fidelity. Noticeably, the reason for the difficulty of aerobics coordination of 10 athletes and the confusion of actual coordination is small, and the accuracy of the Beer effect is high. Our method can be applied for body building, and it can provide the foundation for Game Bill.

Elimination of Crosstalk from Digital Closed-Loop Fiber Optic Gyroscopes via Polymorphic Modulation Method

Elimination of Crosstalk from Digital Closed-Loop Fiber Optic Gyroscopes via Polymorphic Modulation Method

Experience in using the RAPD-PCR technique for identifying E. coli strains in patients with urinary tract infections

Urinary tract infections (UTIS) in the Russian Federation are widespread and often occur in children, adults, most often affecting people in the most active period of their lives. The course of UTI is determined by a number of predisposing factors, such as age, sex, General condition of the patient, the presence of immunocompromising factors. Uncontrolled use of antibacterial therapy is often a predisposing factor in the chronization of the process in the upper urinary tract.To determine the identity of E. coli strains. In 2019, biological material was collected from 50 patients of «Altai regional hospital for war veterans» with urinary tract infections (chronic pyelonephritis and chronic cystitis) to assess bacteriuria. According to the results of bacteriological research, 23 strains of E. coli were isolated. After that, molecular genetic analysis was performed using the Random Amplified Polymorphic DNA method of E. Coli strains obtained from patients with urinary tract infections, and their identity was evaluated. The results of this study showed that the method used is a reliable method of epidemiological mapping and determination of relationships between closely related varieties and strains of microorganisms isolated from various sources. Carrying out one bacteriological examination of urine in urinary tract infections is not enough, only high-tech techniques allow establishing the exact type of the causative agent of these infections and will allow selecting the correct appointment of antimicrobial drugs in patients with this group of infections.

Development of 31 EST-SNP markers in Glycyrrhiza uralensis Fisch (Leguminosae) based on transcriptomics

Ural licorice, Glycyrrhiza uralensis Fisch (Leguminosae), is an economically important and endangered medicinal plant species in China. Understanding of population genetic structure using single nucleotide polymorphisms (SNPs) will provide valuable knowledge for the conservation and sustainable utilization of the Ural licorice. Base on the transcriptomic data of G. uralensis, a total of 30,030 potential SNPs were identified. Among them, 31 out of 60 selected SNPs were validated by the cleaved amplified polymorphic sequences method in 32 individuals from two wild populations. The expected heterozygosities for these two populations ranged from 0.054 to 0.500 and 0.069 to 0.500, respectively, while the observed heterozygosities ranged from 0.056 to 1.000 and 0.53 to 1.000, respectively. These SNPs will greatly benefit the assessment of genetic diversity of wild populations for G. uralensis.

The prevalence of allelic variants of VKORC1, CYP2C9 and CYP4F2 genes in Zaporizhzhia region population

Objective – to determine the allele frequency of VKORC1, CYP2C9 and CYP4F2 genes in Zaporizhzhia region population. Materials and methods . We investigated 94 individuals at the age of 56 (47; 62) years (men – 23, women – 71) who underwent preventive examination at the “Health” Diagnostic Center based on the ESMC “University Clinic” of the Zaporizhzhya State Medical University. To investigate the polymorphism of CYP2C9, CYP4F2, VKORC1 genes the polymorphic chain reaction method was used. Results. It was established that in Zaporizhzhia region population, the frequency of detection of CYP2C9*2 polymorphic variants is: C/C – 80.9 %, C/T – 19.1 %, T/T – not observed; CYP2C9*3: A/A – 89.0 %, A/C – 11.0 %, C/C – not observed; CYP4F2: C/C – 50.0%, C/T – 40.4 %, T/T – 9.6 %; VKORC: 1 G/G – 40.4 %, G/A – 49.0 %, A/A – 10.6 %. It was discovered that the frequency of registration of polymorphic variants of genes CYP2C9, CYP4F2, VKORC1 varies in different geographic regions, that justifies the expediency of determining these genotypes for each population. Conclusions. The implementation of pharmacogenetic testing based on the polymorphism of genes that affect warfarin metabolism for the warfarine dose titration is perspective.

Comparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry Cultivars

Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage of polymorphic using ISSR method was 83%. Five primers of SSR markers were used and they gave 41 DNA bands with 36 polymorphic DNA bands. Percentage of polymorphic using SSR method was 87%. Four primers of PCR-RFLP markers and 3 enzymes were used and they had 20 DNA bands with 9 polymorphic DNA bands. Percentage of polymorphic PCR-RFLP method was 45%. Three dendrogram results based on each method indicated that Stroberi Hitam has smallest similarity index compared with other eleven cultivars. 'Californica' cultivar had the closest similarity with Stroberi Hitam. Data analysis of molecular character requires complementary and supporting data of other characters such as morphology, biochemistry and cytology so this will strengthen the lineage data of the organisms to be investigated. Molecular marker can be used as a good method for genetic variation analysis if it has some advantages such as high polymorphism, codominance and specific characteristics and wide distribution.

Molecular typing of healthcare-associated infection strains with random amplified polymorphic DNA method by two types of capillary electrophoresis analysis

Molecular typing of healthcare-associated infection strains with random amplified polymorphic DNA method by two types of capillary electrophoresis analysis

Evaluation of proteasomal gene polymorphisms in Lithuanian patients with asthma

Objective: To investigate polymorphisms of proteasomal genes PSMA6 (rs1048990 and rs2277460), PSMC6 (rs2295826 and rs2295827) and PSMA3 (rs2348071) in Lithuanian patients with asthma. Methods: One-hundred forty-six asthma patients and 150 control subjects were studied. DNA was extracted from peripheral blood samples. Five single nucleotide polymorphisms (SNP’s) of the three proteasomal genes were analyzed using allele-specific amplification or the cleaved amplified polymorphic sequence method. Results: While certain alleles and genotypes of PSMA6 rs2277460 and rs1048990 and PSMA3 rs2348071 SNP’s occurred more frequently in asthma patients than in controls, no statistically significant differences in alleles or genotypes of PSMA6, PSMC6 or PSMA3 were observed between asthma patients and control subjects. However, when male and female study subjects were considered separately, we found that the CG genotype of PSMA6 rs1048990 is significantly more frequent in male asthma patients compared to male control subjects. Conclusions: We found no significant differences in frequencies of selected five proteasomal gene PSMA6, PSMC6 and PSMA3 SNP’s between asthma patients and control subjects overall. Among male subjects, however, the CG PSMA6 rs1048990 genotype was significantly more frequent in asthma patients compared with control subjects.

Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of theGerbera jamesoniiBolus Line

An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images of the multiplied by means of the semi-specific PCR method DNA fragments are more complex and polymorphic than those obtained through the RAPD method. The studies of the genetic diversification of Gerbera cultivars employing the aforementioned methods made it possible to conduct a concentration analysis and evaluation of the genetic distance between the lines, manifesting at the same time the superiority of the semi-random PCR method. Moreover, it transpired that the use of mixtures of RAPD primers not always leads to an increase of the number of generated polymorphic bands.

Improvement of strain discrimination by combination of RAPD with PFGE for the analysis of the swine isolates of Salmonella enterica serovar Choleraesuis

Salmonella enterica serovar Choleraesuis (S.Choleraesuis) can cause salmonellosis in pigs and humans. Currently, the most common method used for the subtyping of this Salmonella serovar is pulsed field gel electrophoresis (PFGE) using XbaI as a DNA digestion enzyme. In this study, we compared and combined PFGE with the randomly amplified polymorphic DNA method, for the typing of 95 S. Choloraesuis strains isolated from diseased pigs. Using PFGE with XbaI, with AvrII, and with SpeI digested DNA, 29, 74, and 40 patterns, respectively, were obtained. Also, 53, 15, and 35 strains, respectively, belong to the major patterns X1, A1, and S1. When these three digestion patterns were combined, 83 PFGE pattern combinations were obtained. On the other hand, using RAPD with selected primer alone generated 76 patterns, and 11 strains which fell within a single X1A1S1 PFGE combination pattern were discriminated into 10 patterns. Thus, for S. Choloraesuis, PFGE with AvrII allowed higher discrimination than PFGE with XbaI, and some of the PFGE groupings obtained by combining the XbaI, AvrII and SpeI digestion patterns were further subdivided by the RAPD method.

The prevalence of 16S rRNA methylase gene armA and drug resistance in Acinetobacter baumannii

Objective To investigate the prevalence of 16S rRNA methylase gene armA and to analyze their effect on the drug resistance in multi drug-resistant strains of Acinetobacter baumannii . Methods A total of 72 Acinetobacter baumannii isolates were collected from the Second Xiangya Hospital from Jan. 2008 to Dec. 2008. The size of inhibitory zone of these strains to gentamycin, tobramycin and amikacin were determinate using Kirby-Bauer( K-B) method. The 16S rRNA methylase genes armA were detected by PCR. PCR products were purified and sequenced. Then we used randomly amplified polymorphic DNA method (RAPD) genotyping technology for the establishment of DNA fingerprinting. In addition, we compared drug sensitivity test with RAPD technology. Results Twenty isolates of 72 strains were armA positive and the resistance rates of the strains with armA gene to gentamycin, tobramycin, amikacin were 90.0% , 90.0% and 90. 0% , respectivily. armA positive stains were divided into 7 types using RAPD technology. A genotype was the advantage type. Conclusion The study showed that 16S rRNA methylases gene armA was prevalent in Acinetobacter baumannii which could lead to resistant to almost all aminoglycosides at a high level. And the main form of armA gene prevalence in our hospital was the spread of the same clone strain inside and outside of clinic department. Key words: Acinetobacter baumannii; 16S rRNA methylase genes; Drug resistance; armA gene

Trichophyton rubrum and Trichophyton interdigitale: Genetic Diversity Among Species and Strains by Random Amplified Polymorphic DNA Method

Onychomycosis is a common condition that represents up to 50% of all nail problems and 30% of all cases of dermatophytoses. Trichophyton rubrum and Trichophyton interdigitale are the most common agents involved in this condition. In cases of recurrent post-treatment onychomycosis, strain fingerprinting could reveal whether the original isolate is responsible, a new strain has been acquired or if multiple strains are involved. The aim of this study was to evaluate the efficacy of the RAPD method for species and strain differentiation of T. rubrum and T. interdigitale obtained from patients with subungeal distal-lateral onychomycosis. A set of 86 strains of onychomycosis causative dermatophytes were submitted to species differentiation and strain typing by RAPD method with two previously described primers. Both primers proved capable of strain differentiation when tested for each species. Nineteen molecular profiles were configured for T. rubrum isolates with primers 1 and 6. For T. mentagrophytes, ten molecular profiles were configured with primer 1 and twenty-one with primer 6. We found that T. interdigitale and T. rubrum species were grouped in different clusters when both primers were analyzed together. This study shows that these primers are valuable tools for strain differentiation with T. rubrum and T. intedigitale.

Dynamic emulation based modeling and detection of polymorphic shellcode at the network level

It is a promising way to detect polymorphic shellcode using emulation method. However, previous emulation-based approaches are limited in their performance and resilience against evasions. A new enhanced emulation-based detection approach is proposed, including an automaton-based model of the dynamic behavior of polymorphic shellcode and a detection algorithm, the detection criterion of which is derived from that model and ensures high detection accuracy. The algorithm also contains several optimization techniques, highly improving the running performance and the resilience against detection evasion shellcode. We have implemented a prototype system for our approach. The advantages of our algorithm are validated by the experiments with real network data, polymorphic shellcode samples generated by available polymorphic engines and hand-crafted detection evasion shellcode.

Genetic variability of aflatoxin B1 producing Aspergillus flavus strains isolated from discolored rice grains

Twenty-two aflatoxin B1 (AFB1) producing Aspergillus flavus strains were isolated from 1,200 discolored rice grain samples collected from 20 states across India and tested their potential to produce AFB1 on different agar media. Further these isolates were characterized through randomly amplified polymorphic DNA method. All the strains of A. flavus were produced AFB1 on yeast extract sucrose agar media and none of the strains on A. flavus and A. parasiticus agar. Among the 22 strains, two strains from Tamil Nadu (DRAf 009) and Maharashtra (DRAf 015) produced high amount of AFB1 in all the media tested. To assess the genetic variability in A. flavus, the isolates were analyzed by using random amplified polymorphic DNA markers. Isolates showed 17–80% similarity with standard culture of A. flavus (MTCC 2799).

Profiling of environmental Legionella pneumophila strains by randomly amplified polymorphic DNA method isolated from geographically nearby buildings

Legionella pneumophila (L. pneumophila) which is also known as etiologic agent Legionnaires Disease lives in natural water and man made water systems. These bacteria belonging to Legionellaceae family are divided 15 serogroups. Phenotypical methods used for the identification of Legionella isolates are not very discriminatory. In this study we investigated genotypic features of eight L. pneumophila serogroup 1 and 18 L. pneumophila serogroup 2-14 strains isolated from different buildings in Istanbul by randomly amplified polymorphic DNA (RAPD) method. Eight L. pneumophila serogroup 1 strains (37.5%) were similar RAPD profile and they were isolated from buildings located in a short distance (about 500 m). Four L. pneumophila serogroup 2-14 strains (22%) were identical genotypically. Three of these strains were isolated from buildings located in a short distance.

DNA diversity among the free-living amoeba, Naegleria fowleri, detected by the random amplified polymorphic DNA method

Thirty-one Naegleria fowleri strains from different continents were studied by using the Random Amplified Polymorphic DNA (RAPD) method. Five primers among the 40 examined generated eight RAPD variants corresponding partially to their geographic origin. Four characteristic variants are detected in Oceania. The four remaining ones are present in Europe, two of which are observed on other continents. One European variant is found to exhibit similarities with strains from Oceania. The genetic diversity found in Europe and Oceania as well as the existence of ubiquitous RAPD variants bring new insights about the dispersion of N. fowleri throughout the world.

Molecular diversity of species of the Elymus trachycaulus species complex and their relationships to non-North American taxa

Species of the E. trachycaulus complex species are known for their morphological variability, but little is known about their genetic basis. The delimitation of taxa within the complex has been controversial and difficult. E. trachycaulus is predominantly self-pollinating, and lacks clear morphological boundaries between it and E. alaskanus. Another controversial taxonomic issue of E. trachycaulus is the relationships of this complex species to non-North American E. caninus. The objectives of this study were to examine genetic diversity and the systematic relationships among the species of the E. trachycaulus complex and their relationships with E. caninus, E. alaskanus and E. mutabilis. Random amplified polymorphic DNA method was used to study 35 accessions of E. trachycaulus complex and other Elymus species. Higher genetic variation was detected within species of E. trachycaulus complex. Eurasian accessions are as variable as the North American ones. Both UPGMA and NJ analyses did not show clearly separation among species of the E. trachycaulus complex. No clear association between geographic origin and genetic grouping among these species was found. Eurasian E. trachycaulus probably originated from multiple North American populations.

Theoretical Limits on the Number of Electrophoretic Fragments Generated by the Random Amplified Polymorphic DNA Method

Theoretical Limits on the Number of Electrophoretic Fragments Generated by the Random Amplified Polymorphic DNA Method

Factors affecting direct organogenesis from flower explants of Allium giganteum

A novel method is described for the propagation of Allium giganteum Regel using direct organogenesis resulting in multiple shoot structures formed on mature flower buds or ovaries. A two step induction and differentiation procedure, similar to that described earlier in onion, was tested. Flowers were inoculated on the induction medium for 6 days and extracted ovaries were placed on the differentiation medium. Optimal formation of multiple shoot structures was obtained using modified BDS medium containing 50 g l−1 sucrose solidified by a mixture of agar/gellan-gum, with 8.88 μM benzylaminopurine (BA) and 9.05 μM 2,4 dichlorophenoxyacetic acid (2,4-D) in induction medium and 9.08 μM tidiazuron (TDZ) in the differentiation medium. Five plant sources obtained from different European retailers of ornamental bulbs were tested separately. All tested genotypes produced multiple organogenic structures, although induction percentages clustered in two distinctive groups. Shoots formed tended to become dormant, and attempts to improve their growth and rooting included treatment with fluridone. Dormancy was partly broken when shoots were briefly dipped in 1 μM fluridone. Genetic analysis of plant sources using random amplified polymorphic DNA method showed that 5 retailers actually distribute only two different clones, one of them more and the other less responsive to shoot organogensis.