Experience in using the RAPD-PCR technique for identifying E. coli strains in patients with urinary tract infections

Abstract

Urinary tract infections (UTIS) in the Russian Federation are widespread and often occur in children, adults, most often affecting people in the most active period of their lives. The course of UTI is determined by a number of predisposing factors, such as age, sex, General condition of the patient, the presence of immunocompromising factors. Uncontrolled use of antibacterial therapy is often a predisposing factor in the chronization of the process in the upper urinary tract.To determine the identity of E. coli strains. In 2019, biological material was collected from 50 patients of «Altai regional hospital for war veterans» with urinary tract infections (chronic pyelonephritis and chronic cystitis) to assess bacteriuria. According to the results of bacteriological research, 23 strains of E. coli were isolated. After that, molecular genetic analysis was performed using the Random Amplified Polymorphic DNA method of E. Coli strains obtained from patients with urinary tract infections, and their identity was evaluated. The results of this study showed that the method used is a reliable method of epidemiological mapping and determination of relationships between closely related varieties and strains of microorganisms isolated from various sources. Carrying out one bacteriological examination of urine in urinary tract infections is not enough, only high-tech techniques allow establishing the exact type of the causative agent of these infections and will allow selecting the correct appointment of antimicrobial drugs in patients with this group of infections.