Parental products buffer agents viz. calcium phosphate (CP), potassium phosphate (PP) and sodium phosphate (SP) were successfully qualitatively and quantitatively determined on RP-HPLC in the UV detection. The three components were retained in reverse phase by utilizing polymethacrylate resin stationary phase. The quantification was done by using the waters IC-Pak (50 × 4.6) mm, 10 μm column with 1.0 mL/min flow rate. 0.1 M nitric acid solution was used as a mobile phase. The detection was done at 288 nm, column temperature was 30 ºC, injection volume was 50 μL and the total run time was 5 min. The method was validated as per the regulatory and ICH guidelines. The accuracy was found from 98.5% to 101.5% and the precision data was found from 0.5% to 0.9%. The linearity resulted in r value more than 0.999. Quality by design software was used to establish the method robustness. Green chemistry principles and tools were used to assess the method eco-ness. The developed method can be utilized as an application to quantify the buffer agent content in selected parental products.