Natural polymer flocculant possesses an exciting prospect in water treatment due to its non-toxicity, wide source, low cost and biodegradability. In this work, we have successfully synthesized the anionic terpolymer of carboxymethyl cellulose-itaconic acid‑sodium alginate (CIS) by microwave-assisted copolymerization. By studying the flocculation properties towards cationic dye of crystal violet (CV), the optimum synthesis conditions were determined. The maximum removal rate of 100 mg/L CV simulated wastewater was 92.2 % with CIS concentration of 30 mg/L. The flocculation kinetic results showed the rapid dye removal rate and the dye decolorization ratio of 89.8 % could be obtained at 75 s. Moreover, the CIS flocculant showed excellent flocculation effects in ambient pH of 4–10, flocculation temperature of 10–40 °C, and various inorganic salts. In general, the anionic CIS flocculant shows excellent cost effectiveness, where the predicted operation cost of as-prepared CIS is about 60 % of conventional polyacrylamide flocculant. It also has the advantages of excellent ecofriendliness and rich raw material source, indicative its potential applications of wastewater treatment.