Polyherbal Product Research Articles

Evaluation of antioxidant and anti-inflammatory properties, bioactive compound profiling, and molecular mechanisms of a multicomponent Thai herbal formulation

BackgroundMedicinal plants have long been used as dietary supplements to reduce the risk of chronic diseases, inflammation, cancer, and metabolic syndromes. Traditional remedies possess therapeutic properties, making them promising candidates for further investigation and potential pharmaceutical development. Aim of the studyThis study aimed to evaluate the efficacy of Belog Plus (BP), a polyherbal product containing five Thai medicinal plants: Citrus aurantifolia, Cannabis sativa, Tiliacora triandra, Alpinia galanga, and Piper nigrum, combined with safflower seed oil. This study focused on quantifying the total phenolic content (TPC) and total flavonoid content (TFC), and identifying key herbal biomarkers that contribute to its therapeutic properties. In addition to assessing the antioxidant and anti-inflammatory potential of the plant extracts, the study explored the molecular mechanisms underlying their anti-inflammatory activity, offering a comprehensive understanding of BP's efficacy. MethodsThe ethanolic extract of BP and its components were assessed for antioxidant potential using DPPH, FRAP, and H2O2 induced oxidative stress. The anti-inflammatory activity was evaluated using the Griess reaction assay, and the mechanisms were explored through mRNA and protein expression analysis. The TPC and TFC were measured using the Folin–Ciocalteu (F–C) assay and the aluminium chloride colorimetric assay, respectively, while herbal biomarkers were identified using LC-MS/MS analysis. ResultsThe results revealed that the ethanolic extract of BP (6.25-100 µg/ml) exhibited a potent anti-inflammatory effect through nitric oxide inhibition (IC50 24.4 μg/ml) and significantly reducing the expression of inflammatory genes iNOS, COX-2, IL-6, and TNF-α. Additionally, it significantly reduced the expression of inflammatory proteins iNOS and COX-2 at concentrations of 6.25-25 µg/ml. Among the individual components, C. aurantifolia, A. galanga, and P. nigrum showed potent anti-inflammatory effects with IC50 values of 35.7, 6.5, and 23.3 μg/ml, respectively. The TPC and TFC results demonstrated significant levels of phenolic (80.6 µg GAE/mg) and flavonoid (47.4 µg catechin/mg) compounds. Additionally, the LC-MS/MS analysis identified a variety of bioactive compounds known for their anti-inflammatory properties. ConclusionThese findings support BP's potential use as a dietary supplement to mitigate the risk of chronic inflammatory diseases, with its ethanolic extract containing bioactive phenolic and flavonoid compounds that significantly suppress iNOS and COX-2 protein expressions.

BV-4051, Artovid-20®, an Ayurvedic Polyherbal Product Significantly Lowers Interleukin-6 and Symptoms Severity in Moderate SARS-CoV-2 Infected Hospitalized Patients

BV-4051, Artovid-20®, an Ayurvedic Polyherbal Product Significantly Lowers Interleukin-6 and Symptoms Severity in Moderate SARS-CoV-2 Infected Hospitalized Patients

Direct shoot regeneration from cotyledonary node of Uraria picta (Jacq.) Desv. ex DC., an important plant of dashmula drugs, and assessment of genetic fidelity, metabolic profiling, and anti-diabetic activity

Uraria picta root is used in the polyherbal product ‘Dashmula’. Its exploitation for formulation preparation has depleted its availability, leading to medicine adulteration. Direct shoot regeneration from the cotyledonary node holds promise as a source of raw material. This study aimed to develop a regeneration protocol for U. picta and validate its genetic and metabolic fidelity. The seeds of U. picta showed low germination rates, prolonged dormancy, and poor viability. Root exploitation in the wild poses a threat to its availability in nature. Seedling’s derived cotyledonary nodes cultured on B5 medium supplemented with BAP (0.5–3 mg L−1), Kinetin (0.5–3 mg L−1), Thidiazuron (0.01–1 mg L−1), and meta-Topolin (0.1–4 mg L1). To address hyperhydricity in regenerated shoots, cotyledonary nodes were cultured on high-agar concentration media. Microshoots were exposed to IBA solution (50–800 mg L−1) pulse treatment for rooting. Tissue-cultured plants genetic fidelity was assessed using ISSR and SCoT markers, while metabolic fidelity was studied with HRMS. The chlorophyll content, antioxidant, and antidiabetic activity of micropropagated plants were evaluated. The highest shoot regeneration frequency, with a maximum of 6.57±0.278 shoots per explant, was achieved using 2 mg L−1meta-Topolin. The shoots were elongated, had expanded leaves, and were hyperhydrated. BAP (2 mg L−1) induced a maximum of 9.83±0.333 shoot buds per explant. BAP caused explant browning, profuse callus formation, dwarfing, and hyperhydric shoots. Hyperhydricity was alleviated with a higher agar concentration (1 %). IBA (400 mg L−1) induced a maximum of 2.18±0.090 roots per shoot and a root length of 9.23±0.033 cm. Tissue-cultured and mother plants exhibited clonal fidelity, similar metabolite and chlorophyll content, strong antioxidant activity, and equal efficacy for inhibiting α-amylase and α-glucosidase. This method can propagate elite clones of U. picta and offer its improvement via genetic transformation.

A Polyherbal Mixture with Nutraceutical Properties for Ruminants: A Meta-Analysis and Review of BioCholine Powder.

BioCholine Powder is a polyherbal feed additive composed of Achyrantes aspera, Trachyspermum ammi, Azadirachta indica, and Citrullus colocynthis. The objective of this study was to analyze published results that support the hypothesis that the polyherbal product BioCholine Powder has rumen bypass choline metabolites through a meta-analysis and effect size analysis (ES). Using Scopus, Web of Science, ScienceDirect, PubMed, and university dissertation databases, a systematic search was conducted for experiments published in scientific documents that evaluated the effects of BioCholine supplementation on the variables of interest. The analyzed data were extracted from twenty-one publications (fifteen scientific articles, three abstracts, and three graduate dissertations available in institutional libraries). The studies included lamb growing-finishing, lactating ewes and goats, calves, and dairy cows. The effects of BioCholine were analyzed using random effects statistical models to compare the weighted mean difference (WMD) between BioCholine-supplemented ruminants and controls (no BioCholine). Heterogeneity was explored, and three subgroup analyses were performed for doses [(4 (or 5 g/d), 8 (10 g/d)], supplementation in gestating and lactating ewes (pre- and postpartum supplementation), and blood metabolites by species and physiological state (lactating goats, calves, lambs, ewes). Supplementation with BioCholine in sheep increased the average daily lamb gain (p < 0.05), final body weight (p < 0.01), and daily milk yield (p < 0.05) without effects on intake or feed conversion. Milk yield was improved in small ruminants with BioCholine prepartum supplementation (p < 0.10). BioCholine supplementation decreased blood urea (p < 0.01) and increased levels of the liver enzymes alanine transaminase (ALT; p < 0.10) and albumin (p < 0.001). BioCholine doses over 8 g/d increased blood glucose, albumin (p < 0.10), cholesterol, total protein, and globulin (p < 0.05). The ES values of BioCholine in retained energy over the control in growing lambs were +7.15% NEm (p < 0.10) and +9.25% NEg (p < 0.10). In conclusion, adding BioCholine Powder to domestic ruminants' diets improves productive performance, blood metabolite indicators of protein metabolism, and liver health, showing its nutraceutical properties where phosphatidylcholine prevails as an alternative that can meet the choline requirements in ruminants.

Comparative Study of Phytochemicals present in a Polyherbal Anti-diabetic Formulation using Chemical methods and HPTLC methods

The important challenge to Herbal products for their worldwide acceptance is developing the standard methods for their quality assurance. Qualitative and quantitative analysis of the phytochemicals present in any herbal product plays very important role in its further standardisation. BSL-150 is an Indian polyherbal, anti-diabetic tablet formulation which majorly contains leaves of Gymnemasylvestre, seeds of Syzygiumcumini, seeds of Phyllanthusemblica and bark of Tinospora cordifolia. The phytochemical analysis of the marketed tablets and in-house formulation by standard chemical methods showed presence of Alkaloids, Flavanoids, Steroids, Triterpenoids, Saponins, Tannins, Phenols, Phytosterols, Proteins, Anthraquinones, Gums and mucilages. The phytochemical analysis using HPTLC methods showed presence of Flavanoids, Steroids, Triterpenoids, Saponins, Tannins, Phenols, sterols, Anthraquinones, Essential oils, Bitter principles and coumarins. On comparison of the chemical method and HPTLC method for phytochemical screening, the chemical methods are less time consuming but they are not plant specific and general. Whereas, HPTLC methods can be plant specific and can be developed further for polyherbal formulation. Also, on the basis of intensity and Rf of a band, the selection of the phytochemicals as chemical marker for standardisation of the polyherbal product becomes easy. Thus Modern instrumental method of phytochemical screening has more advantages over the chemical method.

Systematic review and network meta-analysis of efficacy and safety of interventions for preventing anti-tuberculosis drug induced liver injury

Anti-tuberculosis drug induced liver injury (Anti-TB DILI) is the most common adverse events (AEs) necessitating therapy interruption but there is no preventing regimen. This study aimed to examine the efficacy and safety of herbs/alternative medicines for preventing anti-TB DILI. Relevant articles were identified through a systematic search in 5 international databases from inception till March 2022. All randomized controlled trials (RCT) assessing the effects of herbal or alternative medicines against anti-TB DILI were included. The network meta-analysis (NMA) was used to synthesize the evidence for preventing hepatotoxicity using a random-effects model. A total of 3423 patients from 14 RCTs were included. The NMA indicated that supplementation of Turmeric plus Tinospora cordifolia (RR 0.07; 95% CI 0.02 to 0.28), and N-acetyl cysteine (NAC) (RR 0.09; 95% CI 0.01 to 0.75) significantly reduced the incidence of anti-TB DILI compared with placebo. In addition, poly herbal product significantly reduced alkaline phosphatase (ALP) (MD − 21.80; 95% CI − 33.80 to − 9.80) and total bilirubin (Tbil) compared with placebo (MD − 0.51; 95% CI − 0.76 to − 0.26). There was no statistically significant difference in the occurrence of AEs in any intervention. In conclusion, Turmeric plus Tinospora cordifolia, NAC and poly-herbal product may provide benefit for preventing anti-TB DILI in TB patients. However, these findings are based on a small number of studies. Additional studies are warranted to confirm the findings.

Control of Chemical Contaminants and Evaluation of Oral Subacute Toxicity of an Alcoholic Polyherbal Product “Plaie de ventre” Marketed for Health Claims in Yopougon (Côte d’Ivoire)

Objective: Alcoholic mixtures are highly appreciated by the Ivorian population to their low cost and for their health claims. Contaminants control and toxicity tests were carried out in order to check the harmlessness of extractable from polyherbal product “Plaie de ventre”.&#x0D; Place and Duration of Study: The study was conducted at the Laboratory of Pharmacology and Clinical Pharmacy of Félix Houphouët-Boigny University for a period of 4 months.&#x0D; Methods: The search for pesticide residues and metallic trace elements was carried out respectively by high-performance liquid phase chromatography and by atomic absorption with air-arc flame ethylene AAS 20, respectively. Subacute oral toxicity study involved daily administration of the extractable from the polyherbal product to three groups of rats for 28 days at doses of 21.06, 42.12, and 84.24 mg/kg bw.&#x0D; Results: The detected residues of pesticides and trace metals in product “Plaie de ventre” mixture were consistent with the standards for their use. Moreover, the administration of extractable from this mixture did not result in any alteration of the body or relative weights of the rats, the structure of the kidney and liver at different doses. Finally, the analysis of the hematological and biochemical parameters indicated a noteworthy escalation (p &lt; 0.05) in levels of white blood cells, blood platelets, and serum triglycerides.&#x0D; Conclusion: The extract from the polyherbal “Plaie de ventre” caused changes in hematological and biochemical parameters compared to control group in treated rats. Consumers may face a long-term risk of developing hyperlipidemia.

Phytochemical investigation and antisickling properties of a Poly-herbal formula on the HBSS Red Blood Cells

The aim of this study was to evaluate the sickle-formation inhibition of a recipe of three plants: Zanthoxylum leprieurii, Xylopia aethiopica and Harungara madagascariensis. The 70% hydroethanolic extract (EZHm) and the decoctate (DZHm) were used to determine the chemical groups by colorimetric and precipitation reactions. These extracts were also used to evaluate the antisickling properties by Emmel&#39;s method, following the protocol described by Image. The biological tests revealed that the total extracts have concentration-dependent and time-dependent anti-sickle cell activity. These extracts act by reducing the polymers of sickle cells. After 120 min the inhibition percentages were 87.35% DZHm, 87.28% EZHm and 85.07% phenylalanine in the presence of 10 mg/mL of the test substances. Plant extracts show a strong antisickling activity which would be due to its phytochemical constituents such as primary (aromatic amino acid) and secondary (alkaloids, phenolic compounds, saponosides, sterols, polyterpenes) metabolites found in these extracts. These extracts, even if they are crude, constitute a hope for the discovery of many antisickling active principles. This natural poly-herbal formula product could improve the quality of life of people with sickle cell disease.

Anti-inflammatory activity of Indonesian polyherbal product containing Curcuma zanthorrhiza and Vitex trifolia as the main ingredients in carrageenan- and histamine-induced inflammation in Wistar rats

Anti-inflammatory activity of Indonesian polyherbal product containing Curcuma zanthorrhiza and Vitex trifolia as the main ingredients in carrageenan- and histamine-induced inflammation in Wistar rats

Study on the Comparative Efficacy and Safety of Intellan, Poly Herbal Product (Test Group) with Vinpocetine (Control Group) to Treat Dementia

This study aimed to compare the efficacy and safety of Polyherbal product Intellan with Vinpocetine in managing dementia and subject them to analysis by Mini metal state examination (MMSE) score, which is the most broadly perceived formal intellectual test. The MMSE was a 30-point test. This study was conducted at the Department of Medicine, Civil Hospital, Karachi, for six months. Patients were dispensed to get the test drug Intellan or controlled drug Vinpocetine. Keeping in consideration for 20% dropout in the test drug arm, with full appliance in the vinpocetine treatment arm, 150 subjects were in each arm. A total of 300 patients of both genders aged between 30 and 75 years who met the inclusion criteria participated in the study. The patients were evaluated prospectively for 24 weeks. The patients were advised follow-up visits weekly. Data were entered and analyzed using SPSS 12.0 (statistical software). The study groups were compared with the baseline characteristic, clinical presentation and history. Mean comparison was done by independent t-test and dependent t-test as appropriate, p≤0.05 was considered significant. No such study can be found that compare these drugs for treating dementia. In our study, mean MMSE before treatment, at 3 months and at 6 months was 8.91±4.12, 10.78±4.41, 13.54±4.54, respectively, while in group B, mean MMSE before treatment, at 3 months and at 6 months was 8.61±4.00, 10.74±4.18, 13.24±4.63, respectively. We found significant mean difference of MMSE before treatment with MMSE at 3 months, MMSE before treatment with MMSE at 6 months and MMSE at 3 months with MMSE at 6 months for both groups. An insignificant mean difference of MMSE at the 6th month s with study group (p=0.572) was found. Mean comparison was also done according to the study group s for stratified categories of gender, age, socio-economic status, education status, family history of dementia, living area, Alzheimer, epilepsy, diabetes mellitus, hypertension and body mass index.

A brief study on standardization and evaluation of ayurvedic preparations of Ushirasava

Ushirasava is the polyherbal hydroalcoholic product prepared from about 27 ingredients. It is a liquid preparation popularly used for bleeding disorders, skin diseases, diabetes and many more. Due to the cooling properties of Ushirasava, it is widely used in the diseases where excessive heat occurs in the body. This study aimed to standardize and evaluate some marketed formulations of Ushirasava with special reference to its physicochemical properties. The organoleptic and physicochemical properties of Ushirasava were standardized with reference to the protocol of testing Ayurvedic, Siddha and Unani medicines. The results were further compared with specifications given in Ayurvedic Pharmacopoeia of India. The results of this study serve a reference tool for standardizing and evaluating formulations of Ushirasava. Keywords: Standardization, Physicochemical, Organoleptic, Fermentation, Bioefficacy

Formulation and Evolution of Polyherbal Soap: A Review Article

Abstract: The polyherbal soap are the one which contains nature herbal ingredients. Many herbal plants and products combines to from polyherbal product. These soaps arise to avoid the synthetic soap. This herbal soap reduces the side effect effects and gives good results to the human. Now a days herbal soap plays major role in the society. It avoids the pathogen infection caused by the environment. Keywords: polyherbal soap, formulation, Antimicrobial

Breaking the challenge of polyherbal quality control using UV and HPLC fingerprints combined with multivariate analysis.

Traditional herbal medicines are mostly composed of more than one herb which act synergistically; hence, there is high demand for proper quality control methods to ensure the consistent quality of polyherbal formulations. Proposing a simple, reliable, and efficient method for the qualitative and quantitative analysis of a polyherbal product using multivariate analysis of ultraviolet-visible (UV-Vis) spectroscopy or HPLC-PDA data. An antiobesity formula consisting of equal proportions of Trachyspermum ammi, Cuminum cyminum, and Origanum majorana was prepared as well as spiked with one of each herb simultaneously, representing accepted and unaccepted samples. Full factorial design (2k ) was applied to study the effect of temperature, sonication, and stirring time for extraction optimisation. The HPLC and UV spectral fingerprints were separately subjected to multivariate analysis. The soft independent modelling of class analogy (SIMCA) and partial least squares (PLS) models were developed to segregate the accepted from the unaccepted samples and to predict the herbal composition in addition to the thymol content in each sample. The SIMCAuv and SIMCAhplc models showed correct discrimination between the accepted and unaccepted samples with excellent selectivity and sensitivity. The PLSuv , PLShplc , and PLSthym models showed excellent linearity and accuracy with R2 > 0.98, slope close to 1, intercept close to 0, low root mean square error of calibration (RMSEC), and root mean square error of prediction (RMSEP) (close to 0). On validation, the PLS models correctly predicted the quantity of the three herbs and thymol content with ±5% accuracy. This study demonstrates the reliability and efficiency of HPLC and UV spectroscopy coupled with multivariate statistical analysis (MVA) for ensuring the consistency of polyherbal preparations.

Toxicological evaluation of a Nigeria-made polyherbal product on selected reproductive functions in adult male Wistar rats

Toxicological evaluation of a Nigeria-made polyherbal product on selected reproductive functions in adult male Wistar rats

Toxicological evaluation of a Nigeria-made polyherbal product on selected reproductive functions in adult male Wistar rats

Ruzu bitters black for men (RBBM) is a polyherbal product widely used amongst men in Nigeria to enhance libido, rejuvenate male organs and to manage erectile dysfunctions, prostate anomalies, weak erection, and premature ejaculation. This study was carried out to investigate the toxicological effect from the use of herbal product. Acute toxicity test of RBBM on rats was carried out in two phases; 10 mg/kg, 100 mg/kg and 1000 mg/kg for phase I and 1600mg/kg, 2900 mg/kg and 5000 mg/kg for phase II, were administered respectively. For sub-acute toxicity, two groups of 5 animals each received RBBM (0.87 mg/kg and1.17 mg/kg respectively) and a third group received water orally for 28 days. The study analyzed the median lethal dosage, and sperm morphology, sperm motility, sperm count, sperm viability and histology of the testes as indices for sub-acute toxicity. No death was recorded for the acute and sub-acute studies but there was a moderate physical sign of toxicity. In the sub-acute toxicity study, there was a significant increase (p˂0.05) in testicular weight of Group 1 animals. Also, sperm count, and sperm motility increases significantly (p˂0.05) while there was a decrease in multiple tail sperm across the test groups. RBBM is not toxic to sperm morphology and causes no death at 5000 mg/kg in male albino Wistar rats.

Sub-Acute Toxicity Assessment of a Polyherbal Product “Combo” on Liver and Kidney Function Indices in Experimental Rats

Sub-Acute Toxicity Assessment of a Polyherbal Product “Combo” on Liver and Kidney Function Indices in Experimental Rats

PHARMACEUTICO- ANALYTICAL STUDY OF BANDANAARU (BRIDELIA SCANDENS R.) GRANULES – A MODIFIED ETHNO-MEDICINE

Folklore medicines broadly mean all aspects of direct relationship of man with plants. It expands its scope into the use of plants as medicine, food, fibres, dyes, tans, taboos, avoidances, and even magico religious beliefs. Ayurveda is known for the use of poly-herbal formulations and multi-component therapeutics for the management of health and diseases. The overall aim of the drug standardization is to ensure the quality, efficacy and the uniformity of the products in terms of their chemical and biological properties. Bandanaaru [Bridelia scendens (Roxb) Willd.] is one of the mostly practised folklore drugs for its nutritional value. It is believed to be equivalent to breast milk to the kids. Along with this drug, the drugs like Bala and Shritala are also added and nutritional granule is prepared based on Standard operative procedure [SOP] of granules mentioned in API. This article reports on standardisation of Bandanaaru granules a polyherbal product used as a nutritive supplement. The prepared granule has shown pleasant sweet taste with good palatability. Preliminary qualitative phytochemical studies have shown the presence of Carbohydrates, Proteins, Starch, Flavonoids, Steroids, Vitamins etc. Keywords: Folklore, Bandanaaru (Bridelia scandens), Bala, Shritala, SOP

Clinical Evaluation of the Safety and Effectiveness of Heptonica: A Ghanaian Hepatorestorative Polyherbal Product.

The incidence of liver diseases is increasing globally, and many patients in developing countries are resorting to the use of herbal products as treatment. This study was aimed at establishing the safety and effectiveness outcomes for patients with deranged liver panel treated with a Ghanaian finished polyherbal product. The product Heptonica is prepared by CPMR from three medicinal plants: Bidens pilosa, Citrus aurantifolia, and Trema orientalis. Fifty (50) participants with clinical and biochemical signs of liver impairment were purposively recruited and treated for a period of 28 days. Participants received Heptonica at a dose of 30 mL 8 hourly after meals for the treatment period. Clinical and biochemical evaluation (liver panel test, renal function test, haematology, and urinalysis) of subjects for the safety and effectiveness of the product was undertaken at days 0 (baseline), 14, and 28. Compared to the baseline values, Heptonica did not have any untoward effect on renal function, haematological parameters, and urine parameters of subjects. Clinical and liver panel results of the participants also improved compared to the baseline: serum aspartate transaminase (AST) (p < 0.0001), alanine transaminase (ALT) (p < 0.0001), gamma-glutamyltransferase (GGT) (p- 0.0013), total bilirubin (p-0.0136), direct bilirubin (p < 0.0001), total proteins (p-0.0409), and alkaline phosphates (p- 0.0284). Level of albumin showed no significant difference within the study period. The outcome of this study indicates Heptonica has hepatorestorative action with no observable toxicity and can be used with confidence as indicated as a liver tonic.

Effect of dietary inclusion of a commercial polyherbal formulation on some physiological and immune parameters in healthy and stressed hens

The effects of the dietary inclusion of a polyherbal formulation based on three powdered herbs (W. somnifera, T. cordifolia, O. sanctum) on some physiological and immune parameters were studied in healthy and stressed laying hens. The effects of the dietary polyherbal formulation were also compared with those of dietary ascorbic acid (AA) supplement, nowadays considered one of the most potent immunostimulant substances widely used as a food supplement.Experimental data did not show any positive effects, or very low ones, on the assessed parameters in healthy hens as a consequence of the two dietary supplementations. On the contrary, the dietary inclusion of the polyherbal mixture or AA partially counteracted the adverse effects in hens subjected to a moderate and transient dexamethasone-induced stress, when ameliorating effects on natural IgM antibody level, specific antibody response, total immunoglobulin content, respiratory burst activity and total antioxidant capacity were shown. The obtained results justify the ethnomedical use of this polyherbal mixture in stressed laying hens in which faster recovery has been demonstrated, whereas healthy specimens did not seem to substantially benefit from the dietary integration, neither with the polyherbal product nor with AA. Thus, the presence of nutraceutical compounds in several herbal plants exerting no side-effects might be useful for exploring them as an alternative to allopathic substances for preventive or therapeutic purposes in poultry.

Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

BackgroundTo investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions.MethodsAntinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue.ResultsTADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment.ConclusionThese results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.