Degradation Of Polyethylene Terephthalate Research Articles

Hydrolase and plastic-degrading microbiota explain degradation of polyethylene terephthalate microplastics during high-temperature composting

This research aims to explore the degradation properties of polyethylene terephthalate (PET) by PET hydrolase (WCCG) in high-temperature composting and its impact on microbial communities. PET degradation, composting parameters and microbial communities were assessed in 220 L sludge composters with PET and WCCG using high-throughput sequencing. Results showed that WCCG addition led to a deceleration of the humification process and a reduction in the relative abundance of thermophilic genera. Potential PET degrading microbiota, e.g. Acinetobacter, Bacillus, were enriched in the plastisphere in the composters where PET reduced by 26 % without WCCG addition. The external introduction of the WCCG enzyme to compost predominantly instigates a chemical reaction with PET, concurently curtailing the proliferation of plastic-degrading bacteria, leading to a 35 % degradation of PET. Both the WCCG enzyme and the microbiota associated with plastic-degradation showed the potential for reducing PET, offering a novel method for mitigating pollution caused by environmental microplastics.

Enhancing polyethylene terephthalate conversion through efficient microwave-assisted deep eutectic solvent-catalyzed glycolysis

Chemical recycling of plastics is a promising approach for effectively depolymerizing plastic waste into its constituent monomers, thereby contributing to the realization of a sustainable circular economy. Glycolysis, which converts polyethylene terephthalate (PET) into the monomer bis(2-hydroxyethyl) terephthalate (BHET), has emerged as a cost-effective and commercially viable chemical recycling process. However, glycolysis requires long reaction times and high energy consumption, limiting its industrialization. In this study, we develop an energy-efficient microwave-assisted deep eutectic solvent-catalyzed glycolysis method to degrade PET effectively and rapidly, resulting in a high BHET yield. This combined approach enables the quantitative degradation of PET within 9 min, achieving a high BHET yield of approximately 99% under optimal reaction conditions. Furthermore, the proposed approach has a low specific energy consumption (45 kJ/g) and minimizes waste generation. The thermal behavior of PET and its degradation mechanism are systematically investigated using scanning electron microscopy and density functional theory-based calculations. The results obtained suggest that the proposed straightforward, swift, and energy-efficient strategy has the potential to offer a sustainable solution to plastic waste management challenges and expedite the industrialization of chemical recycling.

UV Irradiation of Polyethylene Terephthalate and Polypropylene and Detection of Formed Microplastic Particles Down to 1 μm.

The degradation of plastics upon UVC irradiation in aqueous solution and the formation of microplastic (MP) particles were investigated. Polypropylene (PP) and recycled and virgin polyethylene terephthalate (PET) were irradiated with a UV lamp emitting light at 254 nm. Irradiation was performed for 15 and 30 min, respectively, at an intensity of about 0.3 W cm-2 . The formation of MP was studied by Raman spectroscopy. The results showed that MP particles were formed after irradiation and that their number was significantly higher in the recycled PET than in the virgin material. The number of PP MP formed was lower compared to PET and was not significantly different after 15 and 30 min. In addition, ethanol was used as an alternative solvent to investigate how its chemical properties and interactions with UVC irradiation affect the degradation of PET and PP plastics. The use of ethanol and recycled PET resulted in a lower number of MP particles at both irradiation times. When ethanol was used after 30 min of irradiation, significantly more PP MP formed. The different chemical structures of PET and PP combined with the different solvent properties of water and ethanol contribute to the differences in their susceptibility to UVC degradation.

Expression and engineering of PET-degrading enzymes from Microbispora, Nonomuraea, and Micromonospora.

Mismanagement of PET plastic waste significantly threatens human and environmental health. Together with the relentless increase in plastic production, plastic pollution is an issue of rising concern. In response to this challenge, scientists are investigating eco-friendly approaches, such as bioprocessing and microbial factories, to sustainably manage the growing quantity of plastic waste in our ecosystem. Industrial applicability of enzymes capable of degrading PET is limited by numerous factors, including their scarcity in nature. The objective of this study is to enhance our understanding of this group of enzymes by identifying and characterizing novel enzymes that can facilitate the breakdown of PET waste. This data will expand the enzymatic repertoire and provide valuable insights into the prerequisites for successful PET degradation.

Enzymatic Degradation of PET plastic

Polyethylene terephthalate (PET) plastic is one of the most commonly used polymers worldwide and found in high rates as environmental waste. Previous studies have shown that the degradation of plastics using commercial grade enzymes is possible and highly effective in lab settings. However, the effectiveness and rates of enzymatic plastic degradation at environmentally relevant conditions is less known. In this study, we set up a series of sacrificial incubation experiments using a commercial enzyme, Humicola insolens cutinase (HiC), to examine the effect of various environmental variables, including temperature, pH, and salinity, on the hydrolytic degradation of PET. This was performed by measuring the mass loss at different time points during degradation, dissolved organic carbon produced in solution by PET hydrolysis, the Fourier transform infrared (FTIR) spectra of the PET surfaces, and scanning electron microscope (SEM) images of the PET surfaces. The results indicate that the degradation rate is 15-times faster at 55 °C than at 40 °C at pH 8 (1.93 mg day-1 versus 0.13 mg day-1), giving an initial estimate for the activation energy of PET hydrolysis of 2.2 kJ mol-1. In the 55 °C experiment which ran for 10 days, there was a noticeable decrease in the plastic strength and deformation of the plastic after 1 week of degradation. In the 40 °C experiment (duration 16 weeks), FTIR spectral changes were observed as early as week 6, with peaks of interest at 2,970 cm-1, 2,350 cm-1, 1,240 cm-1 , and between 1,300-1,000 cm-1. Ongoing experiments with pH and salinity will provide insight into their effects on the PET degradation rate. Altogether, these results will provide a comprehensive framework for predicting PET degradation rates, and by extension, other plastics that are degraded by hydrolysis, at environmentally relevant pH, temperature, and salinity conditions. In addition, these results provide insight into the effect of degradation on the chemical spectra of plastics and microplastics.

Computation-Based Design of Salt Bridges in PETase for Enhanced Thermostability and Performance for PET Degradation.

Polyethylene terephthalate (PET) is one of the most widely used plastics, and the accumulation of PET poses a great threat to the environment. IsPETase can degrade PET rapidly at moderate temperatures, but its application is greatly limited by the low stability. Herein, a molecular dynamics (MD) simulations combined with a sequence alignment strategy was adopted to introduce salt bridges into the flexible region of IsPETase to improve its thermal stability. In the designed variants, the Tm values of IsPETaseI168R/S188D and IsPETaseI168R/S188E were 7.4 and 8.7 °C higher than that of the wild type, respectively. The release of products degraded by IsPETaseI168R/S188E was 4.3 times that of the wild type. Tertiary structure characterization demonstrated that the structure of the variants IsPETaseI168R/S188D and IsPETaseI168R/S188E became more compact. Extensive MD simulations verified that a stable salt bridge was formed between the residue R168 and D186 in IsPETaseI168R/S188D, while in IsPETaseI168R/S188E an R168-D186-E188 salt bridge network was observed. These results confirmed that the proposed computation-based salt bridge design strategy could efficiently generate variants with enhanced thermal stability for the long-term degradation of PET, which would be helpful for the design of enzymes with improved stability.

Development of a plastic waste treatment process by combining deep eutectic solvent (DES) pretreatment and bioaugmentation with a plastic-degrading bacterial consortium

Polyethylene terephthalate (PET), a petroleum-based plastic, and polylactic acid (PLA), a biobased plastic, have a similar visual appearance thus they usually end up in municipal waste treatment facilities. The objective of this project was to develop an effective PET and PLA waste treatment process that involves pretreatment with deep eutectic solvent (DES) followed by biodegradation with a plastic-degrading bacterial consortium in a composting system. The DES used was a mixture of choline chloride and glycerol, while the bacterial strains (Chitinophaga jiangningensis EA02, Nocardioides zeae EA12, Stenotrophomonas pavanii EA33, Gordonia desulfuricans EA63, Achromobacter xylosoxidans A9 and Mycolicibacterium parafortuitum J101) used to prepare the bacterial consortium were selected based on their ability to biodegrade PET, PLA, and plasticizer. The plastic samples (a PET bottle, PLA cup, and PLA film) were pretreated with DES through a dip-coating method. The DES-coated plastic samples exhibited higher surface wettability and biofilm formation, indicating that DES increases the hydrophilicity of the plastic and facilitates bacterial attachment to the plastic surface. The combined action of DES pretreatment and bioaugmentation with a plastic-degrading bacterial consortium led to improved degradation of PET and PLA samples in various environments, including aqueous media at ambient temperature, lab-scale traditional composting, and pilot-scale composting.

Insights into the Enhancement of the Poly(ethylene terephthalate) Degradation by FAST-PETase from Computational Modeling.

Polyethylene terephthalate (PET) is the most abundant polyester plastic, widely used in textiles and packaging, but, unfortunately, it is also one of the most discarded plastics after one use. In the last years, the enzymatic biodegradation of PET has sparked great interest owing to the discovery and subsequent mutation of PETase-like enzymes, able to depolymerize PET. FAST-PETase is one of the best enzymes hitherto proposed to efficiently degrade PET, although the origin of its efficiency is not completely clear. To understand the molecular origin of its enhanced catalytic activity, we have carried out a thorough computational study of PET degradation by the FAST-PETase action by employing classical and hybrid (QM/MM) molecular dynamics (MD) simulations. Our findings show that the rate-limiting reaction step for FAST-PETase corresponds to the acylation stage with an estimated free energy barrier of 12.1 kcal mol-1, which is significantly smaller than that calculated for PETase (16.5 kcal mol-1) and, therefore, supports the enhanced catalytic activity of FAST-PETase. The origin of this enhancement is mainly attributed to the N233K mutation, which, although sited relatively far from the active site, induces a chain folding where the Asp206 of the catalytic triad is located, impeding that this residue sets effective H-bonds with its neighboring residues. This effect makes Asp206 hold a more basic character compared to the wild-type PETase and boosts the interaction with the protonated His237 of the catalytic triad in the transition state of acylation, with the consequent decrease of the catalytic barrier and acceleration of the PET degradation reaction.

Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches.

Polyethylene terephthalate (PET) hydrolase enzymes show promise for enzymatic PET degradation and green recycling of single-use PET vessels representing a major source of global pollution. Their full potential can be unlocked with enzyme engineering to render activities on recalcitrant PET substrates commensurate with cost-effective recycling at scale. Thermostability is a highly desirable property in industrial enzymes, often imparting increased robustness and significantly reducing quantities required. To date, most engineered PET hydrolases show improved thermostability over their parental enzymes. Here, we report engineered thermostable variants of Ideonella sakaiensis PET hydrolase enzyme (IsPETase) developed using two scaffolding strategies. The first employed SpyCatcher-SpyTag technology to covalently cyclize IsPETase, resulting in increased thermostability that was concomitant with reduced turnover of PET substrates compared to native IsPETase. The second approach using a GFP-nanobody fusion protein (vGFP) as a scaffold yielded a construct with a melting temperature of 80°C. This was further increased to 85°C when a thermostable PETase variant (FAST PETase) was scaffolded into vGFP, the highest reported so far for an engineered PET hydrolase derived from IsPETase. Thermostability enhancement using the vGFP scaffold did not compromise activity on PET compared to IsPETase. These contrasting results highlight potential topological and dynamic constraints imposed by scaffold choice as determinants of enzyme activity.

Discovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties

Excessive polyethylene terephthalate (PET) waste causes a variety of problems. Extensive research focused on the development of superior PET hydrolases for PET biorecycling has been conducted. However, template enzymes employed in enzyme engineering mainly focused on IsPETase and leaf-branch compost cutinase, which exhibit mesophilic and thermophilic hydrolytic properties, respectively. Herein, we report a PET hydrolase from Cryptosporangium aurantiacum (CaPETase) that exhibits high thermostability and remarkable PET degradation activity at ambient temperatures. We uncover the crystal structure of CaPETase, which displays a distinct backbone conformation at the active site and residues forming the substrate binding cleft, compared with other PET hydrolases. We further develop a CaPETaseM9 variant that exhibits robust thermostability with a Tm of 83.2 °C and 41.7-fold enhanced PET hydrolytic activity at 60 °C compared with CaPETaseWT. CaPETaseM9 almost completely decompose both transparent and colored post-consumer PET powder at 55 °C within half a day in a pH-stat bioreactor.

α-Synuclein: A fusion chaperone significantly boosting the enzymatic performance of PET hydrolase

Extensive use of polyethylene terephthalate (PET) has brought about global environmental problems. A recently reported PET hydrolase (PETase) discovered from Ideonella sakaiensis showed high potential for degrading PET at moderate temperatures, but its activity and stability need further improvement for practical applications. Herein, we proposed to use α-synuclein (αS) as a fusion chaperone and created six PETase-αS fusion enzymes with linkers of different types and lengths. All the fusion enzymes exhibited improved enzymatic performance, presenting 1.5 to 2.6-fold higher activity towards bis-2(hydroxyethyl) terephthalate than PETase, as well as significantly increased stabilities. Fluorescence spectroscopy indicated that the chaperone fusion tightened the overall conformation and resulted in the opening of the substrate binding pocket, which led to the improved thermal stability and catalytic activity of the fusion enzymes. Remarkably, one of the fusion proteins, PETase-[(GS)(EK)]10-αS, showed 3.2 to 5.1 times higher PET degradation capability than PETase. The significantly boosted PET degradation performance was not only attributed to the enhanced enzymatic activity and stability, but also possibly due to the binding affinity of the fused αS domain for PET. These findings demonstrated that αS was an effective fusion chaperone for significantly enhancing the enzymatic performance of PETase.

Biodegradation of polyethylene terephthalate (PET) by diverse marine bacteria in deep-sea sediments.

PET plastic waste entering the oceans is supposed to take hundreds of years to degrade and tends to accumulate in the deep sea. However, we know little about the bacteria capable of plastic degradation therein. To determine whether PET-degrading bacteria are present in deep-sea sediment, we collected the samples from the eastern central Pacific Ocean and initiated microbial incubation with PET as the carbon source. After enrichment with PET for 2 years, we gained all 15 deep-sea sediment communities at five oceanic sampling sites. Bacterial isolation for pure culture and further growth tests confirmed that diverse bacteria possess degradation ability including Alcanivorax xenomutans BC02_1_A5, Marinobacter sediminum BC31_3_A1, Marinobacter gudaonensis BC06_2_A6, Thalassospira xiamenensis BC02_2_A1 and Nocardioides marinus BC14_2_R3. Furthermore, four strains were chosen as representatives to reconfirm the PET degradation capability by SEM, weight loss and UPLC-MS. The results showed that after 30-day incubation, 1.3%-1.8% of PET was lost. De-polymerization of PET by the four strains was confirmed by the occurrence of the PET monomer of MHET and TPA as the key degradation products. Bacterial consortia possessing PET-degrading potential are prevalent and diverse and might play a key role in the removal of PET pollutants in deep oceans.

Assessment of Microplastic Degradation in Bottom Sediments Using Raman Microspectroscopy and Atomic Force Microscopy

The assessed degradation of microplastics from bottom sediments of the Laptev Sea, the Weddell Sea, the Chukchi Sea and Peter the Great Bay, Sea of Japan, was studied using Raman microspectroscopy and atomic force microscopy (AFM). The degree of amorphous-crystallinity of polyethylene terephthalate (PET) was determined by the ratio of Raman mode intensities (I1095 cm–1 / I1115 cm–1). Changes in the topography and mechanical properties of the surface of polymer fragments were studied by AFM methods. It has been shown that the degradation of PET found in the bottom sediments of the Laptev Sea affects mainly its crystalline phase. The amorphous phase of PET is affected to a much lesser extent or not at all. The same changes in the phase composition of the PET surface have been shown after plastic exposure in a marine aquarium in silty sand from the Amur Bay (Peter the Great Bay, Sea of Japan). AFM data show an increase in PET surface roughness and a decrease in elasticу modulus (Young’s modulus) in bottom sediment samples. Raman microspectroscopy also shows signs of degradation of cellophane fragments from the Weddell Sea, polyvinyl chloride (PVC) from the Laptev Sea, and polyethylene (PE) from the Amur Bay.

Degradation of PET – Quantitative estimation of changes in molar mass using mechanical and thermal characterization methods

Polyethylene terephthalate (PET) films are used when mechanical strength, thermal and chemical stability, and barrier properties to atmospheric gases are required in combination with good processability. Hydrolysis leading to embrittlement of the material is a major concern as PET is used in a variety of applications with expected lifetimes of up to decades (e.g., for use in buildings, textiles, or photovoltaic backsheets). Therefore, a comprehensive understanding of the degradation processes and the effects on the molecular mass distribution is of great importance.Usually, the direct determination of molar mass and molar mass distribution involves high effort and sophisticated equipment. Therefore, the main objective of this work is to quantify molar mass changes due to accelerated aging using thermal and mechanical methods. Two stabilized PET films were subjected to seven different accelerated aging conditions (heat; combined heat-humidity). The samples were then characterized by size exclusion chromatography (SEC), tensile tests and differential scanning calorimetry (DSC).A linear correlation was found between crystallization temperature and average molar mass. The values of fracture stress from tensile tests indicate a ductile-brittle transition at a molar mass of 15 000 g mol−1. The study concludes that the crystallization temperature obtained from DSC measurements can be used to estimate changes in the average molar mass of PET after hydrolysis. Crystallization temperatures between 208 °C and 211 °C correspond to a critical reduction in molar mass and severe embrittlement.

Molecular mechanism underlying high-affinity terephthalate binding and conformational change of TBP from Ideonella sakaiensis

Ideonella sakaiensis is the bacterium that can survive by degrading polyethylene terephthalate (PET) plastic, and terephthalic acid (TPA) binding protein (IsTBP) is an essential periplasmic protein for uptake of TPA into the cytosol for complete degradation of PET. Here, we demonstrated that IsTBP has remarkably high specificity for TPA among 33 monophenolic compounds and two 1,6-dicarboxylic acids tested. Structural comparisons with 6-carboxylic acid binding protein (RpAdpC) and TBP from Comamonas sp. E6 (CsTphC) revealed the key structural features that contribute to high TPA specificity and affinity of IsTBP. We also elucidated the molecular mechanism underlying the conformational change upon TPA binding. In addition, we developed the IsTBP variant with enhanced TPA sensitivity, which can be expanded for the use of TBP as a biosensor for PET degradation.

Responses of gut microbiomes to commercial polyester polymer biodegradation in Tenebrio molitor Larvae

Polyethylene terephthalate (PET) is a mass-produced fossil-based plastic polymer that contributes to catastrophic levels of plastic pollution. Here we demonstrated that Tenebrio molitor (mealworms) was capable of rapidly biodegrading two commercial PET resins (microplastics) with respective weight-average molecular weight (Mw) of 39.33 and 29.43 kDa and crystallinity of 22.8 ± 3.06% and 18 ± 2.25%, resulting in an average mass reduction of 71.03% and 73.28% after passage of their digestive tract, and respective decrease by 9.22% and 11.36% in Mw of residual PET polymer in egested frass. Sequencing of 16 S rRNA gene amplicons of gut microbial communities showed that dominant bacterial genera were enriched and associated with PET degradation. Also, PICRUSt prediction exhibited that oxidases (monooxygenases and dioxygenases), hydrolases (cutinase, carboxylesterase and chitinase), and PET metabolic enzymes, and chemotaxis related functions were up-regulated in the PET-fed larvae. Additionally, metabolite analyses revealed that PET uptake caused alterations of stress response and plastic degradation related pathways, and lipid metabolism pathways in the T. molitor larvae could be reprogrammed when the larvae fed on PET. This study provides new insights into gut microbial community adaptation to PET diet under nutritional stress (especially nitrogen deficiency) and its contribution to PET degradation.

Expression, purification and characterization of a novel bis (hydroxyethyl) terephthalate hydrolase from Hydrogenobacter thermophilus

PET (polyethylene terephthalate) is one of the most important petrochemicals that is widely used in mineral water bottles, food and beverage packaging and textile industry. Because of its stability under environmental conditions, the massive amount of PET wastes caused serious environmental pollution. The use of enzymes to depolymerize PET wastes and upcycling is one of the important directions for plastics pollution control, among which the key is the depolymerization efficiency of PET by PET hydrolase. BHET (bis(hydroxyethyl) terephthalate) is the main intermediate of PET hydrolysis, its accumulation can hinder the degradation efficiency of PET hydrolase significantly, and the synergistic use of PET hydrolase and BHET hydrolase can improve the PET hydrolysis efficiency. In this study, a dienolactone hydrolase from Hydrogenobacter thermophilus which can degrade BHET (HtBHETase) was identified. After heterologous expression in Escherichia coli and purification, the enzymatic properties of HtBHETase were studied. HtBHETase shows higher catalytic activity towards esters with short carbon chains such as p-nitrophenol acetate. The optimal pH and temperature of the reaction with BHET were 5.0 and 55 ℃, respectively. HtBHETase exhibited excellent thermostability, and retained over 80% residual activity after treatment at 80 ℃ for 1 hour. These results indicate that HtBHETase has potential in biological PET depolymerization, which may facilitate the enzymatic degradation of PET.

Heterologous expression and characterization of Aquabacterium parvum lipase, a close relative of Ideonella sakaiensis PETase in Escherichia coli

Polyethylene terephthalate (PET) is a globally mismanaged plastic waste. Microbial degradation of plastics is a green and sustainable technology and very few studies report biocatalysts with plastic degradation potential. The present study aims to investigate the PETase from the evolution of IsPETase. Through NCBI database scanning, Aquabacterium parvum lipase (ApLip) was identified as an evolutionary intermediate between conventional lipases and IsPETase based on 16 S rRNA, amino acid sequences and protein structural similarity by simulation. The codon adaptation index (CAI) was used for ApLip to obtain ApLip-Ec and its protein expression was enhanced in Escherichia coli Lemo21(DE3) with a high catalytic efficiency of 11341 mM−1 min−1 based on p-nitrophenyl butyrate (pNPB) as a substrate. In vitro testing for PET degradation using 5.5 µM ApLip-Ec released 5.9 µM BHET after 48 h. Moreover, the synergism effect of ApLip-Ec and MHETase was explored. Successful PET degradation not only existed in Ideonella sakaiensis, but also evolved from structurally similar cutinases and lipases in nature.

Evaluation of microplastic contamination by metals in a controlled environment: A risk to be considered

The metal contamination and the degradation of polyethylene terephthalate (PET) due to human activities have contributed to the worsening of environmental problems in aquatic systems. Therefore, the study aimed to evaluate PET microplastic adsorption levels when exposed to high amounts of Ni, Cu and Co. The PET microplastic was characterized by scanning electron microscopy, Brunner-Emmet-Teller, porosimetry system, Barrett-Joyner-Halenda and Fourier transform infrared spectroscopy with attenuated total reflectance for evaluation of surface morphology, surface area, porosity, pore size and functional groups, respectively. The results showed that the surface area, the presence of macro and mesopores, and the functional groups influence the adsorption of metals on the surface of PET microplastic. The adsorption isotherms confirmed the presence of mesoporosity and macroporosity on the PET microplastic surface. The Freundlich and Langmuir models were used to study the adsorption capacity. The kinetics of adsorptions were interpreted using pseudo-first order and pseudo-second order models. The results indicated that the Langmuir isotherm and the pseudo-second order adequately described the adsorption of metals by the PET microplastic. The removal rates by the PET microplastic varied from 8 to 34% for Ni, 5 to 40% for Cu and 7 to 27% for Co after a period of 5 days. Furthermore, the adsorption was predominantly chemical and extremely fast, indicating that the presence of microplastics in the environment can lead to a rapid metal accumulation which elevates the hazards potential of microplastic in living beings.

Switchable enzyme mimics based on self-assembled peptides for polyethylene terephthalate degradation

Polyethylene terephthalate (PET), the most abundant polyester plastic, has become a global concern due to its refractoriness and accumulation in the environment. In this study, inspired by the structure and catalytic mechanism of the native enzyme, peptides, based on supramolecular self-assembly, were developed to construct enzyme mimics for PET degradation, which were achieved by combining the enzymatic active sites of serine, histidine and aspartate with the self-assembling polypeptide MAX. The two designed peptides with differences in hydrophobic residues at two positions exhibited a conformational transition from random coil to β-sheet by changing the pH and temperature, and the catalytic activity followed the self-assembly “switch” with the fibrils formed β-sheet, which could catalyze PET efficiently. Although the two peptides possessed same catalytic site, they showed different catalytic activities. Analysis of the structure − activity relationship of the enzyme mimics suggested that the high catalytic activity of the enzyme mimics for PET could be attributed to the formation of stable fibers of peptides and ordered arrangement of molecular conformation; in addition, hydrogen bonding and hydrophobic interactions, as the major forces, promoted effects of enzyme mimics on PET degradation. Enzyme mimics with PET-hydrolytic activity are a promising material for degrading PET and reducing environmental pollution.