Abstract In this paper the response of the plasma poloidal rotation to resonant magnetic perturbations (RMPs) is investigated in EAST Tokamak using the multi-channel Doppler Backscattering (DBS) system. It shows that the poloidal rotation spins up towards the ion-diamagnetic drift direction with increasing external perturbation field, which will reduce the edge shear. In ohmically heated discharges, the n=1 RMP can only affect the edge poloidal rotation when the RMP coil current is small, and the influence will gradually reach the inner regions with increasing RMP coil current. At the moment of the n=1 RMP penetration, all the poloidal rotations measured by the DBS will increase significantly, and then they will keep almost unchanged with the increase of the RMP coil current. In H-mode discharges, the poloidal rotation is significantly influenced by the n=2 RMP, and the edge velocity well even reverses, along with ELMs mitigation. However, in the same shot, the n=4 RMP with the same coil current amplitude can hardly affect the poloidal rotations and the behavior of ELMs.