Low 25‐hydroxyvitamin D3 (25‐OH‐D3) concentration in pregnancy increases the risk of preeclampsia (PE) based on studies from countries with vitamin D fortification policy. The objective of this study is to determine if vitamin D intake and status is associated with PE in a country without vitamin D fortification. A case control study (n=62) of pregnancies with (case) and without (control) PE was conducted in Serbia in the winter. Maternal and cord blood samples from delivery were measured for plasma 25‐OH‐D3, 3‐epi‐25‐hydroxyvitamin D3 (3‐epi‐25‐OH‐D3) and 24,25‐dihydroxyvitamin D3 (24,25‐(OH)2D3) by LC‐MS/MS. Group differences were tested with ANOVA, Bonferroni post hoc test and P<0.05. Exogenous vitamin D intake was not different but women with PE delivered infants at younger mean gestational age and had lower plasma 25‐OH‐D3, 3‐epi‐25‐OH‐D3 and 24,25‐(OH)2D3 (table). Case infants were of lower birth weight (Case: 2622 ± 796 vs Control: 3388 ± 381 g, p<0.001), not different in total plasma 25‐OH‐D3 (Case: 9.38 ± 1.05 vs Control: 11.17 ± 0.99 ng/ml, p=1.000) but with higher proportion of 3‐epi‐25‐OH‐D3 (Case: 7.95 ± 0.22 vs Control: 7.01 ± 0.21 % of total 25‐OH‐D3, p=0.016). A high prevalence of vitamin D deficiency, defined by plasma 25‐OH‐D<12 ng/ml, was observed in 56% in mothers and 84% in infants. These data underscore the importance of prenatal supplementation and food fortification policy in Serbia. Mothers’ characteristics Control group n=32 Case groupn=30 mean SD mean SD P value Age (years) 27.7 4.8 29.1 5.8 0.374 Vitamin D intake Supplemental (IU/day) 198.2 140.4 173.3 163.9 0.453 Gestational age at delivery (weeks) 37.7 0.5 36.3 2.1 0.029 25‐OH‐D3 (ng/ml) 16.09 5.65 11.23 5.12 0.002 3‐epi‐25‐OH‐D3 (ng/ml) 0.70 0.21 0.51 0.53 0.008 24,25‐(OH)2D3 (ng/ml) 0.80 0.34 0.76 0.57 1.000