Interaction Point Research Articles

An Analysis of Determinants of Consumer Dissatisfaction towards Plastic Packaging Containing Ready-to-Eat and Serve Meal

Packaging is an essential aspect of product and its protection, serving as the first point of interaction for consumers. It encompasses a diverse range of materials and designs, tailored to meet the specific needs of different industries and products. For this study, a total sample of 500 respondents was collected. Data were analyzed by using structural equation modeling technique (SEM). Our analysis suggests that functional value is not found to have significant impact on consumer attitude towards plastic packaging while sensory appeal, food safety concern and social value has significant impact on consumer attitude towards plastic packaging. Results show that consumers are worried about safety of food. Primary purpose of packaging is to keep food protected from spoilage and contamination by elements such as air, light, moisture and pests. Research shows that consumers tend to associate food packaged in non-plastic materials with higher status as compared to food packed in plastic packaging, evoking a premium perception linked to elevated societal class. Consumer perceptions of food packed in plastic packaging are significantly shaped by the social value attributed to packaging materials, influenced by environmental awareness and social status. Sensory appeal plays a crucial role in influencing consumer attitudes towards packaging, especially when it comes to ready-to-eat or ready-to-serve food products. The packaging of a product can have a substantial impact on a consumer's perception of the food's quality, freshness, and overall desirability. The results of our study further show that consumer attitude towards food in plastic packaging has a significant impact on consumer dissatisfaction. The study underscores three key implications: first, marketers should prioritize health-friendly packaging materials for safety, second innovation in packaging should enhance both product protection and sensory appeal, and third, Government intervention is essential to address food safety concern and protect consumers and the food supply chain.

Discovering quirks through timing at FASER and future forward experiments at the LHC

Quirks are generic predictions of strongly-coupled dark sectors. For weak-scale masses and a broad range of confining scales in the dark sector, quirks can be discovered only at the energy frontier, but quirk-anti-quirk pairs are produced with unusual signatures at low pT, making them difficult to detect at the large LHC detectors. We determine the prospects for discovering quirks using timing information at FASER, FASER2, and an “ultimate detector” in the far-forward region at the LHC. NLO QCD corrections are incorporated in the simulation of quirk production, which can significantly increase the production rate. To accurately propagate quirk pairs from the ATLAS interaction point to the forward detectors, the ionization energy loss of charged quirks traveling through matter, the radiation of infracolor glueballs and QCD hadrons during quirk pair oscillations, and the annihilation of quirkonium are properly considered. The quirk signal is separated from the large muon background using timing information from scintillator detectors by requiring either two coincident delayed tracks, based on arrival times at the detector, or two coincident slow tracks, based on time differences between hits in the front and back scintillators. We find that simple cuts preserve much of the signal, but reduce the muon background to negligible levels. With the data already collected, FASER can discover quirks in currently unconstrained parameter space. FASER2, running at the Forward Physics Facility during the HL-LHC era, will greatly extend this reach, probing the TeV-scale quirk masses motivated by the gauge hierarchy problem for the broad range of dark-sector confining scales between 100 eV and 100 keV.

Modulation of Tannic Acid on the Cholesteric Structure of Cellulose Nanocrystals.

The chiral nematic phase structure, formed by the self-assembly of cellulose nanocrystals (CNCs) in an aqueous suspension and maintained in a solid film, shows great potential for optical applications. To achieve complex structures in optical devices, it is crucial to subject CNCs to specific shearing processes, such as spinning and printing. Understanding the structural and property changes of the CNC liquid crystal phase in these processes is of utmost importance. In this study, we investigated the effect of adding tannic acid (TA) on the rheological properties and cholesteric phase structures of CNCs/TA mixed suspensions. By calculating the surface site interaction points, we observed that TA can adsorb onto the surface of CNC rods in suspensions through hydrogen bonding. Through characterization techniques, such as polarized optical microscopy, rheology, and synchrotron SAXS, we examined the effects of TA addition on the microstructure and rheological properties of the CNC liquid crystal phase and clarified the change relating to the system composition. Under the same CNC concentration, the volume fraction of the anisotropic phase, the pitch, and the rod spacing of the cholesteric phase were not significantly affected by the addition of TA. However, the system viscosity was significantly reduced with the appropriate amount of TA (2 wt %), in a wide range of CNC concentrations (up to 15 wt % CNCs). The flow indexes (n) in Region I and Region III of steady-state shear curves of CNCs/TA systems (11-15 wt % CNCs) were compared. Moreover, we introduced the well-established theoretical models for liquid crystal polymers to tentatively interpret Region I of the CNCs/TA cholesteric phase and realized that increased numbers of smaller cholesteric-phase domains in the CNCs/TA system and interfacial modification by TA may contribute to the fluidity change. The feature of the domain texture of CNCs/TA systems is verified by polarized optical microscopy observations.

A C++ program for estimating detector sensitivities to long-lived particles: displaced decay counter

A series of far-detector programs have been proposed for operation at various interaction points of the large hadron collider during the upcoming runs. Investigating the potential and complementarity of these experiments for new-physics searches goes through the estimation of their sensitivity to specific long-lived particle models. Here, we present an integrated numerical tool written in the C++ language and called Displaced Decay Counter, which we have created to this end and which can be used in association with MadGraph5, Pythia8, or any other state-of-the-art Monte-Carlo collider simulation tool. Several far-detector models have been implemented within the program, accounting for the geometry and integrated luminosity of projected detectors. Additional or more accurate designs can be easily constructed through a dedicated interface. The functionality of this tool is exemplified through the discussion of several benchmark scenarios, which we consider for the validation of the implemented detector models.

Update of the 5 MW Beam-on-Target Requirements for improvement of the materials irradiation performance at IFMIF-DONES

IFMIF-DONES is a facility under construction in Granada, whose main goal is the validation and characterization of materials under a fusion prototypic irradiation field. This field is created by the interaction of a high energy intense continuous deuteron beam and a flowing liquid lithium target. The requirements imposed on the beam at the interaction point are a complex trade-off among the scientific experimental needs for the materials irradiation defined at the top-level requirements (20 dpa in a volume of 0.3 dm3 and 50 dpa in 0.1 dm3), and the technical constraints of several systems such as the Accelerator Systems, the Lithium Systems, and the Test Systems. Recent simulations with the initial definition of beam-on-target requirements showed the necessity of redefining them in order to fulfill the irradiation needs. This contribution will address the main challenges to gather the inputs for the definition and reassessment of the beam-on-target requirements. A comparison detailing the main changes compared to the previous ones will be given, together with a short overview of the studies ongoing by different systems to analyze the impact of each beam-on-target requirements on the performance of the whole facility.

Insight into the molecular basis of the soluble storage-state to fibrous-state structural transformation of spider pyriform silk

Pyriform (or piriform) silk is a fibrous spider silk used in a composite material to attach web silks to substrates and disparate silk types together. Argiope argentata pyriform silk is formed from a protein with a central repetitive region consisting of a 234-amino acid unit (the “Py unit”) repeated 21 times. We have previously shown that the Py unit behaves modularly, with a structured core of 6 α-helices that retains its structuring upon truncation of N- and C-terminal intrinsically disordered segments. Here, we apply solution-state nuclear magnetic resonance (NMR) spectroscopy to investigate the structured core of the Py unit. Based on 15N spin relaxation measurements and reduced spectral density mapping at two field strengths, the α-helical core of the Py unit exhibits minimal backbone motion beyond the tumbling of the globular domain except for one inter-helical linker that exhibits a notably higher degree of dynamics. Evaluation of rotational diffusion based on 15N spin relaxation and translational diffusion by pulsed field gradient diffusion NMR demonstrates that the core of the Py unit behaves as a compact structure, with hydrodynamic behaviour consistent with a well-packed protein. Pyriform silk protein undergoes a structural transformation upon conversion from the soluble-state to the fibrous-state, with a loss of α-helical content and gain of β-sheet content. The more dynamic segment we observe midway through the structured core of the Py core, especially in the context of the intrinsically disordered segments that flank the core, would provide an initiation point for decompaction, structural transformation, and/or intermolecular interactions.

Long-Term Advantages of Adolescent Optimism: Nonlinear Associations With Adult Outcomes and its Protective Role in Buffering Socioeconomic Risk

This study examined whether optimism in early adolescence predicts occupational and psychosocial outcomes in early adulthood and explored the functional form of this relation. We also investigated whether these associations continue to hold after accounting for concomitant factors and whether optimism acts as a protective factor that helps early adolescents deal with socioeconomic adversity. We followed a large sample of German seventh graders ( N = 1596; 63.8% females; baseline M age = 12.9) at two measurement points over a period of 18 years and estimated latent regression models. Optimism in early adolescence predicted several adult outcomes, including occupational prestige, social integration, psychosocial symptoms, and depression. Analyses with social integration, depression, and life satisfaction revealed a nonlinear association: Optimism promoted life outcomes, but this positive association reached a plateau in above-average ranges of optimism and a minimum value in below-average optimism ranges. Moreover, optimism in early adolescence buffered the negative effects of low parental socioeconomic status on occupational prestige, job satisfaction, and psychosomatic symptoms in adulthood. The findings offer increased knowledge about the long-term significance of optimism and underscore the necessity of considering these effects from a more comprehensive and interactional point of view.

Quality Improvement of Urban Public Space from the Perspective of the Flâneur

As the quality of public space has become significant for urban development, the creation of high-quality public spaces is becoming increasingly important. Since the implementation of urban renewal policies, an increasing number of buildings have emerged, creating new types of public spaces. Compared to original public spaces, new public spaces are more open, flexible, and diverse. The design of public spaces is closely related to users and the flâneur can precisely serve as a user and observer to conduct in-depth research. So, our study was conducted under the identity of the flâneur, focusing on two cases in Guangzhou. The flâneur completes the data collection through two methods. Static research involves observing and taking photos, whereas dynamic research involves interviews and questionnaires. This study analysed three aspects: the group category, behavioural diversity, and activity time and evaluated the public space using the NPS scale. The study found that the recommendation rate of new urban public spaces is higher than that of original public spaces. The study also found that original public spaces need to be improved in four ways: equipping furniture facilities, improving traffic congestion, increasing blue-green spaces, and establishing artistic spaces. New urban public spaces need to make efforts to create more interactive spaces and increase stagnation points.

A Cloud-Based WEB Platform for Fall Risk Assessment Using a Therapist-Centered User Interface Which Enables Patients’ Tracking Remotely

This work describes a system to help in the remote assessment of fall risk in elderly people. A portable hardware system equipped with an RGB-D sensor is used for motion capture. A set of anonymous frames, representing the process of skeleton tracking, and a collection of sequences of interesting features, obtained from body landmark evaluations through time, are stored in the Cloud for each patient. A WEB dashboard allows for tailored tests to be designed, which include the typical items within well-known fall risk evaluation tests in the literature. Such a dashboard helps therapists to evaluate each item from the analysis and observation of the sequences and the 3D representation of the body through time, and to compare the results of tests carried out in different moments, checking on the evolution of the fall risk. The software architecture that implements the system allows the information to be stored in a safe manner and preserves patients’ privacy. The paper shows the obtained results after testing an early prototype of the system, a discussion about its advantages, and the current limitations from the Human–Computer Interaction point of view, and a plan to deploy and evaluate the system from the usability perspective in the near future.

USE OF AUDIOMARKETING TO PROMOTE THE SERVICES OF THE TOURISM INDUSTRY

The aim of the study is to substantiate the importance and peculiarities of using audio marketing tools to promote tourism services in the tourism industry. The article proves that the use of neuroimaging methods in marketing is gaining popularity, as it provides marketers with information that cannot be obtained using conventional marketing methods. Musical content is increasingly integrated into the marketing communications system, covers the points of interaction with tourists, and contributes to the personalization of audiocommunication. It has been determined that audio marketing is an important element of modern marketing strategies, and its tools not only influence the behavior and psycho-emotional state of consumers of tourism services, but also significantly enhance the effectiveness of other marketing tools aimed at obtaining the expected result. Well-chosen music helps to improve interaction and form a positive perception of tourism products and services among tourists, creates sound comfort, ensures a good mood, enhances positive impressions, builds commitment, increases loyalty, extends the time of stay, and increases both planned and spontaneous purchases. The article substantiates that the choice of background music is of great importance in the formation of audio design, which provides tourists with positive emotions, creates an appropriate atmosphere and a pleasant overall impression. It is proved that in order to achieve an effective result of music's influence on consumers of tourist services, producers of tourist services need to constantly conduct marketing research on the needs and behavior of tourists; monitor and analyze the impact of music on tourist behavior; and conduct training and professional development of marketers.

Fuzzy Based Expert System For Test Case Generation On Web Graphical User Interface For Usability Test Improvement

The Ease of Use Test (UT) method is used to evaluate a website's or its point of interaction's usability without involving the site's actual users. UT can be carried out manually or with the use of a machine. Currently, a lot of software testers manually test their program, which leads to issues like lengthier test times, uneven testing, and the requirement for human intervention to complete every test. The ease of use test manual is an expensive and time-consuming process. Analyzers are additional resources needed for manual labor, and there is a great chance that these results will conflict. The goal of this investigation is to improve the reliability and skill of the Test Case (TC) age experiments; the test system is delivered using test instruments that have been programmed. The purpose of this examination's efficient writing audit was to identify any gaps in the current AT and create a mess in the TC era. The evaluation was also focused on identifying the primary issues raised by alternate neighborhood analysts throughout the physical creation of TC. According to the selected plausible experiments, TC was created using the fluffy rationale master structure. Non-probabilistic, vulnerability-related, and multi-esteemed reasoning can all be emphasized in fluffy reasoning. The purpose of the information inquiry was to obtain access to the login page and to create experiments about Graphic User Interface events using a lighthearted justification. The framework separated the conditions, traits, and watchwords from the information examination code, and the outcomes were displayed as experiments. A close examination of behavioral test system age processes was conducted using the master framework for evaluation based on fluff. The evaluation results obtained through quantifiable analysis demonstrated that the provided framework is significantly more productive and reliable for conducting experiments than the manual framework.

Beam-induced backgrounds measured in the ATLAS detector during local gas injection into the LHC beam vacuum

Inelastic beam-gas collisions at the Large Hadron Collider (LHC), within a few hundred metres of the ATLAS experiment, are known to give the dominant contribution to beam backgrounds. These are monitored by ATLAS with a dedicated Beam Conditions Monitor (BCM) and with the rate of fake jets in the calorimeters. These two methods are complementary since the BCM probes backgrounds just around the beam pipe while fake jets are observed at radii of up to several metres. In order to quantify the correlation between the residual gas density in the LHC beam vacuum and the experimental backgrounds recorded by ATLAS, several dedicated tests were performed during LHC Run 2. Local pressure bumps, with a gas density several orders of magnitude higher than during normal operation, were introduced at different locations. The changes of beam-related backgrounds, seen in ATLAS, are correlated with the local pressure variation. In addition the rates of beam-gas events are estimated from the pressure measurements and pressure bump profiles obtained from calculations. Using these rates, the efficiency of the ATLAS beam background monitors to detect beam-gas events is derived as a function of distance from the interaction point. These efficiencies and characteristic distributions of fake jets from the beam backgrounds are found to be in good agreement with results of beam-gas simulations performed with the Fluka Monte Carlo programme.

Role of Recreational Facilities in Community Well-Being in India

Purpose: The aim of the study was to assess the role of recreational facilities in community well-being in India. Methodology: This study adopted a desk methodology. A desk study research design is commonly known as secondary data collection. This is basically collecting data from existing resources preferably because of its low cost advantage as compared to a field research. Our current study looked into already published studies and reports as the data was easily accessed through online journals and libraries. Findings: The study revealed that, ranging from parks and playgrounds to community centers and sports complexes, serve as focal points for social interaction, physical activity, and cultural exchange within neighborhoods. Studies indicate that access to such amenities correlates with improved mental and physical health outcomes, reduced stress levels, and increased community cohesion. Furthermore, recreational facilities often contribute to crime prevention by providing safe spaces for leisure activities and fostering a sense of belonging among residents. Their presence can also enhance property values and attract businesses, thus contributing to overall community development. As vital components of urban planning and public infrastructure, investing in and maintaining recreational facilities is essential for fostering healthier, happier, and more connected communities. Implications to Theory, Practice and Policy: Social capital theory, place attachment theory and ecological systems theory may be used to anchor future studies on assessing the role of recreational facilities in community well-being in India. Design and implement community-driven initiatives to enhance the accessibility, inclusivity, and quality of recreational facilities, ensuring that they meet the diverse needs and preferences of residents across different demographic groups and geographic contexts. Advocate for policies and funding mechanisms that prioritize investments in recreational infrastructure as integral components of urban planning and development strategies, recognizing their essential role in promoting public health, social cohesion, and economic vitality.

Numerical Simulation of Extreme Ice Loads on Complex Pile Legs of Offshore Substation Structures

The sea ice failure mode and ice force amplitude depend on the structural form at the point of interaction, but the impacts of ice load when interacting with marine engineering structures with additional attachments are not yet clear. This study conducts numerical simulations using the discrete element method to investigate the interaction between sea ice and cable pipes attached to offshore substation structures. Various operating conditions such as ice velocity, ice thickness, and ice attack angle are selected to simulate the interaction between sea ice and such structures, clarifying the variations in the sea ice failure mode and ice force amplitude. The results indicate that crushing failure mainly occurs when sea ice interacts with such structures, and the presence of cable pipes does not alter the sea ice failure mode at the legs of offshore substation structures. The preliminary action of sea ice with cable pipes effectively reduces the ice load on the structure, and the minimum ice force amplitude occurs at an ice attack angle of 90°, with the ice force amplitude increasing with the ice thickness but showing no clear correlation with the ice velocity. The findings of this study provide a reference for the ice-resistant design of offshore substation structures in cold regions.

Improvements in charged lepton and photon propagation for the software PROPOSAL

Accurate particle simulations are essential for the next generation of experiments in astroparticle physics. The Monte Carlo simulation library PROPOSAL is a flexible tool to efficiently propagate high-energy leptons and photons through large volumes of media, for example in the context of underground observatories. It is written as a C++ library, including a Python interface. In this paper, the most recent updates of PROPOSAL are described, including the addition of electron, positron, and photon propagation, for which new interaction types have been implemented. This allows the usage of PROPOSAL to simulate electromagnetic particle cascades, for example in the context of air shower simulations. The precision of the propagation has been improved by including rare interaction processes, new photonuclear parametrizations, deflections in stochastic interactions, and the possibility of propagating in inhomogeneous density distributions. Additional technical improvements regarding the interpolation routine and the propagation algorithm are described. New version program summaryProgram Title: PROPOSAL.CPC Library link to program files:https://doi.org/10.17632/g478pjdcxy.2.Developer's repository link:https://github.com/tudo-astroparticlephysics/PROPOSAL.Licensing provisions: LGPL.Programming language: C++, Python.Journal reference of previous version: Comput. Phys. Commun. 242 (2019) 132.Does the new version supersede the previous version?: Yes.Reasons for the new version: Substantial addition of features. Various bugfixes.Summary of revisions: The library now also treats photons and has the corresponding processes implemented. New parametrizations for photonuclear interaction have been implemented. The angular deflection in stochastic energy losses has been implemented in addition to the already existing multiple scattering implementation, which has been improved to reduce the runtime. The implementation of the Landau-Pomeranchuk-Migdal effect has been corrected. The propagation algorithm has been improved, including the support of inhomogeneous density distributions.Nature of problem: Three-dimensional propagation of charged leptons and photons through different media. Particles lose energy stochastically by ionization, bremsstrahlung, pair production, and photonuclear interaction for charged leptons (including annihilation with atomic electrons for positrons) and Compton scattering, pair production, photoelectric effect and photohadronic interaction for photons. Additionally, they are deflected while propagating through the medium due to both multiple elastic Coulomb scattering as well as deflections in individual stochastic interactions. Unstable particles eventually decay, producing secondary particles.Solution method: Monte-Carlo simulation. The library samples the next interaction point, the type of interaction process, the energy lost in this interaction process, and the energy lost until this point. Particles are propagated until they decay, lose all their kinetic energy (for photons: reach a lower energy limit defined by the validity of the underlying cross sections), or until a user-defined termination criterion is reached. For each propagation step, the angular deflection and endpoint shift due to multiple scattering is calculated.To improve the performance and deal with the divergence of the bremsstrahlung cross section for small photon energies, energy losses below a predefined relative or absolute energy threshold are treated continuously. The computation time is improved by the use of interpolation tables.

The Liquid Jet Endstation for Hard X-ray Scattering and Spectroscopy at the Linac Coherent Light Source

The ability to study chemical dynamics on ultrafast timescales has greatly advanced with the introduction of X-ray free electron lasers (XFELs) providing short pulses of intense X-rays tailored to probe atomic structure and electronic configuration. Fully exploiting the full potential of XFELs requires specialized experimental endstations along with the development of techniques and methods to successfully carry out experiments. The liquid jet endstation (LJE) at the Linac Coherent Light Source (LCLS) has been developed to study photochemistry and biochemistry in solution systems using a combination of X-ray solution scattering (XSS), X-ray absorption spectroscopy (XAS), and X-ray emission spectroscopy (XES). The pump–probe setup utilizes an optical laser to excite the sample, which is subsequently probed by a hard X-ray pulse to resolve structural and electronic dynamics at their intrinsic femtosecond timescales. The LJE ensures reliable sample delivery to the X-ray interaction point via various liquid jets, enabling rapid replenishment of thin samples with millimolar concentrations and low sample volumes at the 120 Hz repetition rate of the LCLS beam. This paper provides a detailed description of the LJE design and of the techniques it enables, with an emphasis on the diagnostics required for real-time monitoring of the liquid jet and on the spatiotemporal overlap methods used to optimize the signal. Additionally, various scientific examples are discussed, highlighting the versatility of the LJE.

3-D deformation inversion: A MATLAB toolbox for automatically calculating SAR-derived 3-D deformation maps of glacier, landslide, and land subsidence

Geological bodies prone to disasters, such as glaciers, landslides, and land subsidence, undergo three-dimensional (3-D) movement. Spaceborne Synthetic Aperture Radar (SAR) satellites commonly capture relative directional motion for Earth observation. However, this begs the question of how to track the 3-D movement of geological bodies. Presented here, the 3-D Deformation Inversion toolbox MATLAB-based concurrently processes ascending and descending SAR-derived datasets acquired from either Pixel Offset Tracking (POT) or Differential Interferometric SAR (DInSAR) methodology, in addition, generates long-term 3-D deformation and interactive point time series and line section information, also dynamic map visualizations. It is the ability to calculate the least squares solution using truncated or multi-order Tikhonov regularized Singular Value Decomposition (SVD). Three various scenarios are employed to assess processing capabilities. The L-curve method finds the optimal calculation parameters tailored to various objects. The toolbox's effectiveness and applicability enhance the potential for evolutionary dynamic analysis in geoscience.

Open-source graphical user interface for the creation of synthetic skeletons for medical image analysis

Open-source graphical user interface for the creation of synthetic skeletons for medical image analysis

Experimental Demonstration of a Large Transverse Emittance Ratio 11∶1 in the Relativistic Heavy Ion Collider for the Electron-Ion Collider.

The Electron-Ion Collider (EIC), to be constructed at Brookhaven National Laboratory, will collide polarized high-energy electron beams with hadron beams, achieving luminosities of up to 1.0×10^{34} cm^{-2} s^{-1} in the center-of-mass energy range of 20-140GeV. To achieve such high luminosity, the EIC will employ small and flat beams at the interaction point. In the hadron storage ring of the EIC, the ratio of horizontal to vertical emittances is approximately 11∶1. In contrast, in previous or existing hadron colliders, the horizontal and vertical emittances are typically similar or closely matched. At the Relativistic Heavy Ion Collider (RHIC), we experimentally demonstrated a large transverse emittance ratio of 11∶1 with gold ion beams at a particle energy of 100GeV per nucleon, thanks to stochastic cooling and fine decoupling. Furthermore, we demonstrated collisions with flat beams, featuring a transverse beam size ratio of 3∶1 for the first time at the RHIC.

Development, feeding, and sex shape the relative quantity of the nutritional obligatory symbiont Wolbachia in bed bugs.

The common bed bug, Cimex lectularius, is a hemipteran insect that feeds only on blood, and whose bites cause public health issues. Due to globalization and resistance to insecticides, this pest has undergone a significant and global resurgence in recent decades. Blood is an unbalanced diet, lacking notably sufficient B vitamins. Like all strict hematophagous arthropods, bed bugs host a nutritional symbiont supplying B vitamins. In C. lectularius, this nutritional symbiont is the intracellular bacterium Wolbachia (wCle). It is located in specific symbiotic organs, the bacteriomes, as well as in ovaries. Experimental depletion of wCle has been shown to result in longer nymphal development and lower fecundity. These phenotypes were rescued by B vitamin supplementation. Understanding the interaction between wCle and the bed bug may help to develop new pest control methods targeting the disruption of this symbiotic interaction. The objective of this work was thus to quantify accurately the density of wCle over the life cycle of the host and to describe potential associated morphological changes in the bacteriome. We also sought to determine the impact of sex, feeding status, and aging on the bacterial population dynamics. We showed that the relative quantity of wCle continuously increases during bed bug development, while the relative size of the bacteriome remains stable. We also showed that adult females harbor more wCle than males and that wCle relative quantity decreases slightly in adults with age, except in weekly-fed males. These results are discussed in the context of bed bug ecology and will help to define critical points of the symbiotic interaction during the bed bug life cycle.