SUMMARY Mycoplasma were isolated from the lungs of 10 calves suffering from pneumonia and similar organisms were recovered from 46 nasal swabs taken from animals suffering from a respiratory infection. By means of complement fixation, agglutination, growth inhibition and fluorescent antibody techniques, the strains were classified into 2 distinct groups. The majority (group A) consisting of both lung and nasal isolates, belonged to an unknown serotype; the rest (group B), consisting only of nasal strains, had the characters of Mycoplasma laidlawii. Serological examinations of paired bovine serum samples showed that somewhat higher agglutinating antibody titres against the unknown Mycoplasma were present in animals from 2 farms, where this strain of Mycoplasma had been recovered, than from a farm where the organism was not isolated. No complement fixing antibodies against this Mycoplasma were detected in any of the animals. The significance of these results is discussed in relation to the question of the possible role of the unidentified Mycoplasma in the aetiology of calf pneumonia.
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