Plinia Peruviana Research Articles

Ultrasound-assisted extraction and concentration of phenolic compounds from jabuticaba sabará (Plinia peruviana (Poir.) Govaerts) peel by nanofiltration membrane

Jabuticaba peel, rich in antioxidants, offering health benefits. In this study, the extraction of phenolic compounds from jabuticaba peel using ultrasound-assisted (UA) and their subsequent concentration by nanofiltration (NF) employing a polyamide 200 Da membrane was evaluated. The UA extractions were conducted using the Central Composite Rotatable Design (CCRD) 22 methodology, with independent variables extraction time (11.55 to 138 min) and temperature (16.87 to 53.3 °C), and fixed variables mass to ethanol solution concentration at pH 1.0 (1:25 g/mL), granulometry (1 mm), and ultrasonic power (52.8 W). The maximum concentrations obtained were 700.94 mg CE/100 g for anthocyanins, 945.21 mg QE/100 g for flavonoids, 133.19 mg GAE/g for phenols, and an antioxidant activity IC50 of 24.36 μg/mL. Key phenolic compounds identified included cyanidin-3-glucoside, delphinidin-3-glucoside, and various acids like syringic and gallic. NF successfully concentrated these compounds, enhancing their yield by up to 45%. UA and NF integrate for sustainable extraction.

Diameter growth and age of jaboticaba trees (Plinia peruviana (Poir.) Govaerts) native from Southwest of Paraná, Brazil, based on growth-rings.

The jaboticaba tree (Plinia peruviana (Poir.) Govaerts) is a native Brazilian species, and its fruits are very popular in Brazil. The purpose of this study was to model the diameter growth, and determine the age of jaboticaba trees in four natural populations in Southwest of Paraná State, Brazil. The knowledge of the growth dynamics of this species is essential to assist management and conservation strategies and to discover when this species was established in the region. Core samples were collected to obtain complete growth series by measurement of annual growth rings. The series were crossdated, seven growth models were adjusted, and the best model for each site and a general model were selected using statistics rules. The time series spanned periods of ~75 to ~100 years, and growth ranged between 0.27 and 0.37 cm year-1. Chapman-Richards' model showed better adherence for locations individually, and Monomolecular when grouped. Because to the age reached by the jaboticaba trees and the high density of this species in these natural populations, consequently named "jaboticabais", it was inferred that there was an anthropogenic action in the dispersion and formation of the settlements through the indigenous and caboclos that inhabited the region before 1940. Also, this is the first work to register and verify the fusion of trunks for species of the Plinia genus using double piths found in samples, a feature that must be considered to avoid overestimating the age of jaboticaba trees based solely on measuring tree diameter.

Weeds as an essential component of sustainable meliponiculture practices in organic agroecosystem

Knowledge of bee flora is key information for sustainable meliponiculture practices. Aiming to stablish the plant species used by the stingless bee Tetragonisca angustula used as food sources during the year in an organic agroecosystem, this study identified the pollen grains in the pollen loads of worker bees. The pollen load of bees returning to hives was collected bi-monthly for one year, summing 240 pollen loads analyzed by optical microscopy. We found 76 pollen types in the pollen loads, of which 60% were of weeds. The plants most frequently identified in the pollen loads were: Alchornea, Arracacia xanthorrhiza, Baccharis, Bidens pilosa, Brassica juncea, Brassica oleracea, Byrsonima sp., Cecropia, Chamaecrista, Citrus sp., Crotalaria sp., Croton, Datura, Eucalyptus, Hyptis, Lippia alba, Mangifera indica, Melastomataceae sp., Momordica charantia, Myrsine, Pereskia aculeata, Persea americana, Piptadenia, Pisum sativum, Plinia peruviana, Pluchea, Poaceae, Prunus persica, Psidium guajava, Raphanus sativus, Rubus urticifolius, Schefflera, and Schinus. Bees foraged mostly on weeds, especially during winter and autumn, and so the presence of weeds can be very beneficial to meliponiculture practices. The by-products of meliponiculture in agroecosystems are derived from diversified botanical sources, that is, wild honey of good quality because it is from an organic system.

GENETIC DISSIMILARITY IN JABUTICABA (Plinia peruviana (poir.) govaerts) IN NORTH OF STATE ESPÍRITO SANTO, BRAZIL

Jabuticaba (Plinia peruviana (Poir.) Govaerts) is a plant rich in compounds that are very useful in industry. Its fruits are the basis for a range of food and pharmaceutical products. Due to the great potential of this species, information about genetic structure of these plants allows us to understand factors linked to quality, in addition to guiding crosses to improve this species for commercial purposes. Thus, we sought to understand the pattern of genetic diversity of jabuticaba found in the northern region of Espírito Santo State, Brazil, using ISSR markers. The results of the dissimilarity values, obtained by the Jaccard index, grouped the 19 accessions studied into five groups. The pair of accessions 10 and 19 showed the highest dissimilarity value, being indicated in crosses aiming at increasing heterozygosity. Accessions 16 and 18 had a lower dissimilarity value, being promising in introgression and gene association studies, due to the narrow genetic basis between the materials. Accessions 1, 4, 12, and 15 are the most divergent and can be indicated for random artificial crossings.

Morphophysiological adjustments to shade of jaboticaba tree saplings

We aimed to verify the morphophysiological changes in jaboticaba tree (Plinia peruviana (Poir.) Govaerts) saplings under shade gradient to improve knowledge on the appropriate light environmental conditions for the saplings production and field homogeneous or intercropping cultivation of this species. The saplings were grown under full sun and artificial shade levels (30%, 50%, and 80%). Growth, photosynthetic pigments, gas exchanges, chlorophyll fluorescence, and leaf anatomy characters were evaluated. Our results showed that jaboticaba tree saplings growth was improved under full sun and 30% of shade. Under 50% and 80% shade the saplings demonstrate phenotypic plasticity, such as larger and thinner leaves. Chlorophyll fluorescence, chlorophyll content, and quantum yield of photosystem II were higher under higher shade level, but the CO2 assimilation rate was not different between light conditions. These changes are typically found in shade-tolerant plants and is related to perform photosynthesis more efficiently in highly variable light conditions. Despite that, we recommend that cultivation be carried out under full sun or up to 30% shade to maximize jaboticaba tree saplings growth in nurseries and orchards.

Dormancy in native fruit seeds of the Brazilian South Region

The aim of the present study was to investigate whether seeds from six native fruit species ofthe South Brazilian regionhad any type of dormancy, as well as, to verify if they havephotoblastism. The work was carried out at the Plant Physiology Laboratoryofthe Federal Technological University of Paraná –Câmpus Dois Vizinhos, Paraná State, Brazil. The influence of photoperiod factors and techniques for overcoming dormancy were tested on the germination of Eugenia uniflora L.(Surinan cherry), Plinia peruviana(native jabuticaba), Plinia cauliflora(hybrid jabuticaba), Eugenia involucrataDC. (Rio Grande cherry), Myrcianthes pungens(guabiju) and Campomanesia guazumifolia(sete capoteiro). The seeds were exposed in a condition of total darkness or photoperiod of 24 hours, being the levels of one of factors tested. The techniques for breaking dormancy tested werethe immersion in gibberellic acid solution (200 mg L-1); stratification at 5°C for 30 days; immersion in water at room temperature for 24 hours; immersion in hot water (80°C) for five minutes; immersion in sulfuric acid solution (96%) for five minutes; and manual physical scarification with 120 grit sandpaper. The seeds were also tested without using any technique, making up the controlstreatments with or without light. The experimental design was completely randomized, in a 2 x 7 factorial scheme (luminosity x treatment for breaking dormancy), with four replications of 100 seeds. After 60 days, germination (%), percentage of accumulated germination, germination speed index and average germination time were analyzed. The seeds of most of the studied native species do not present dormancy or the effect of photoblastism, except for “sete capoteiro”seedswho are supposed to have morphophysiological dormancy, with negative photoblastism. The use of hot water or sulfuric acid in the seeds of native fruit trees is not recommended.

Caracterização físico-química e desenvolvimento pós-colheita de jabuticabas (Plinia peruviana e P. cauliflora)

Plinia peruviana and P. cauliflora are Myrtaceae with potential for agricultural exploitation or urban afforestation. However, there is not much information about its characteristics; consequently, making the objective of this work to evaluate morphometry (size, fresh fruit mass, pulp, bark and seeds, pulp yield, number of seeds per fruit and fruit color), chemical composition (soluble solids, acid titratable AT, Ratio and vitamin C content) and post-harvest behavior (chemical composition and loss of fresh mass) of its fruits. The samples studied were rounded, with high water content (83%), high yield of pulp (between 67 and 76%), fresh mass between 5.30 and 6.82 g and more than one seed per fruit. They presented between 11.4 and 12.7 SS, low AT and 19 mg 100 g of pulp-1 of vitamin C. Plinia peruviana and P. cauliflora have shown similar physicochemical characteristics and can be stored for 28 days under refrigeration (≈2oC).

Somatic embryogenesis from mature split seeds of jaboticaba (Plinia peruviana (Poir) Govaerts)

Plinia peruviana is a species that is native to Brazil and is important due to the taste and medicinal properties of its fruits. Young leaves and split mature seeds were used as explants to initiate somatic embryogenesis to obtain a large number of plants in a short period of time. Leaf discs were cultured in MS medium containing various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) or picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid). In the case of the mature seeds, various concentrations of glutamine, 2,4-D and a combination of auxin and BAP (6-benzylaminopurine) were tested for somatic embryogenesis induction. For somatic embryo maturation, several concentrations of PEG 6000 (polyethylene glycol; up to 90 g L-1) were tested. After 60 days of culture using leaf discs, callus formation occurred in all treatments, with the highest averages obtained with 10 μM 2,4-D. However, these calluses did not form somatic embryos. For the cultured seeds, the best treatment was the MS medium with 1,000 mg L-1 glutamine and 10 μM 2,4-D without BAP. The supplementation of 60 g L-1 PEG 6000 was sufficient to promote the maturation of the somatic embryos. Histological analyses of the calluses that were formed from leaf discs showed nonembryogenic characteristics. In contrast, the calluses that originated from mature seeds had small and round cells with little vacuolation, which are characteristics of embryogenic structures.

Endophytic bacteria associated with tissue culture and leaves of Plinia peruviana

Abstract: The objective of this work was to isolate endophytic bacteria from tissue culture and leaves of jaboticaba (Plinia peruviana) and to evaluate their potential as plant growth-promoting bacteria. The bacteria were isolated from nodal segments grown in vitro and from leaves from a tree under natural conditions, totaling 11 and 54 isolates, respectively. The isolates were characterized by colony morphology. The indolic compounds produced by the isolates, in the presence or absence of 100 mg L-1 tryptophan, were quantified. The greatest producers of these compounds were identified by sequencing the 16S rRNA gene and were inoculated on jaboticaba seeds, using Azospirillum brasilense (Ab-V6) as a positive control. The sensitivity of bacteria to eight antibiotics was also evaluated. All assessed bacteria produced indolic compounds, especially Bacillus sp., with a content of 27.41 μg mL-1. The germination rate of the seeds inoculated with Stenotrophomonas sp. was high - 97.34% compared with that of 74.67% of the negative control. Bacillus sp. and Stenotrophomonas sp. also sped up germination. Chloramphenicol limited the growth of 82% of the isolates, followed by amoxicillin, gentamicin, levofloxacin, and tetracycline, which limited 70%; erythromycin was only effective against 35%. The endophytic bacteria isolated from jaboticaba show characteristics of plant growth-promoting bacteria, and Bacillus sp. and Stenotrophomonas sp., obtained from tissue culture, are capable of enhancing jaboticaba seed germination.

Potential of the near-infrared spectroscopy for the discrimination of wood and charcoal of four native Myrtaceae species in southern Brazil

ABSTRACT Because of the species similarity and the need to differentiate wood of the Myrtaceae family, this study aimed to evaluate the potential of near-infrared spectroscopy for discrimination of wood and charcoal from Campomanesia xanthocarpa Berg, Eugenia pyriformis Cambess., Myrcia retorta Cambess. and Plinia peruviana (Poir.) Govaerts, native of the Araucaria Forest. For each evaluated species, three trees were collected and a disk at breast height was cut and divided into samples obtained in the region near the pith, intermediate and near the bark. For each sample, two spectra were obtained in each anatomical section (transversal, radial and tangential) in the region from 4000 to 10,000 cm−1. Results indicated the similarity of wood and charcoal spectra of all species, and principal component analysis (PCA) of raw data on samples showed little distinction of species, but second derivative pretreatment provided adequate discrimination. When evaluation was based on anatomical sections and position in trunk, for wood in all positions (near bark, intermediate, near pith) it was possible to separate species, but for charcoal some more samples dispersion were observed. Linear discriminant analysis corroborated the results of PCA. Near-infrared spectroscopy presented adequate results and the potential to help discriminate wood and charcoal of the four Myrtaceae species.

WOOD COLORIMETRY OF NATIVE SPECIES OF MYRTACEAE FROM A ARAUCARIA FOREST

Colorimetric parameters have potential to differentiate tree species through their wood type. Therefore, this can be used to aid the inspection of illegal trade. Thus, considering the need to identify species with precision and speed, this study aimed to explore the potential of these parameters in order to characterize wood of some species of native Myrtaceae from the Araucaria Forest: Campomanesia xanthocarpa Berg., Eugenia pyriformis Cambess., Myrcia retorta Cambess., and Plinia Peruviana (Poir.) Govaert. Twelve trees were collected randomly – three per species – for the removal of a disc at diameter at breast height (DBH). We took samples from three regions of the disc (next to the bark, intermediate and next to the pith) and analyzed them in three anatomical sections (transversal, radial and tangential) by the system CIE L*a*b* using a bench colorimeter. We analyzed the data based on the following criteria: test of means in order to differentiate species, quantitative factor analysis (considering the region of the disc and the anatomical section as factors), and principal components analysis (PCA). Then, we used the original spectrum and the values of parameters L*, a* and b* in order to verify the distribution of the species. The analysis determined that the species present different values for the colorimetric parameters. In addition, the factors region of the disc and anatomical section have specific influence for each species. Finally, the PCA indicated that only E. pyriformis differs from the others.

Vitamin and bioactive compound diversity of seven fruit species from south Brazil.

The consumption of native tropical fruits represents an important source of bioactive food and vitamins for consumers. The aim of this study was to determine the composition of vitamins, bioactive compounds and the antioxidant activity of seven native fruits of the Myrtaceae family from south Brazil. Sample 1 of Eugenia pyriformis presented the highest value of β-carotene (0.1021 g kg-1 ), lutein (0.0511 g kg-1 ), zeaxanthin (0.0370 g kg-1 ), and α-carotene (0.0112 g kg-1 ), of all analyzed samples. The three samples of Eugenia uniflora L presented the highest lycopene (0.1876, 0.1240 and 0.1615 g kg-1 ) and vitamin A content (0.106, 0.035 and 0.178 g kg-1 RAE) of all seven species analyzed. The cyanidin 3-glucoside was the most common anthocyanin found in fruits in the present study. Two samples of Plinia peruviana presented higher antioxidant capacity by the ABTS radical method (0.80 and 0.67 mol TEs kg-1 ) among the fruits analyzed. The samples of Campomanesia xanthocarpa analyzed stood out owing to the high content of vitamin C present (23.89, 36.83 and 35.05 g kg-1 ). The values of pantothenic acid in Plinia peruviana account for 20% of daily requirements. In conclusion, the native fruits studied can provide an appreciable amount of vitamins and bioactive compounds. © 2018 Society of Chemical Industry.

Cow milk enriched with nanoencapsulated phenolic extract of jaboticaba (Plinia peruviana).

This study evaluated the total phenolic content (TPC) and the antioxidant activity (AA) of cow's milk enriched with phenolic compounds extracted from jaboticaba peel, either by adding jaboticaba crude extract or a jaboticaba-loaded nanoemulsion. Three nanoemulsions with 5, 10 and 15% of jaboticaba extract were prepared. Average particle diameter (166.7-181.7nm), polydispersity index (0.138-0.156) and zeta potential (ranging from - 35.30 to - 38.60mV) were measured for the three different colloidal systems. The nanoemulsion with 15% of jaboticaba extract (J15-NE) was chosen for milk enrichment. J15-NE showed an encapsulation efficiency of 85.6% and remained stable for 60days at 8°C. Transmission electron microscopy of J15-NE displayed nanoparticles with a well-defined spherical shape. Reference milk, milk enriched with jaboticaba extract and milk enriched with J15-NE were characterised by a TPC of 93, 171 and 161µg/ml GAE (gallic acid equivalent), respectively, and an AA of 0.04, 0.17 and 0.14µg/ml TEAC (trolox equivalent antioxidant capacity), respectively. Thus, this study showed that nanoemulsion with jaboticaba peel extract could be exploited as an ingredient to enrich the properties of milk.

Parameters of water consumption associated with the microclimate of an orchard of jaboticaba trees in southern Brazil

This work aimed to quantify the evapotranspiration and to evaluate the microclimate of an orchard of jaboticaba trees [Plinia peruviana (Poir.) Govaerts]. Field studies were carried out in Porto Alegre, RS, in humid subtropical climate. The orchard was implanted in 2005, with plant spacing of 4.5m x 4.5m. Air temperature and relative humidity, photosynthetically active radiation (PAR), inside and outside the canopy, and soil moisture were monitored continuously. The evapotranspiration of the crop (ETc) was calculated by the decrease of the soil-water storage. The ETc/ETo ratio was determined by linear regression analysis, with ETo being the reference evapotranspiration. The relative humidity was higher inside than outside the canopy, with similar trend in air temperature. The interception efficiency of PAR increased from 80% in autumn-winter to 92% in spring-summer. ETc followed evaporative demand and leaf area, ranging from 0.3 to 3.2 mm day-1 in winter and from 0.2 to 5.0 mm day-1 in the summer. Most of the soil-water extraction occurred between 0 and 40 cm depth. The ETc/ETo ratio (assumed as Kc coefficient) was 0.95, ranging from 0.90 in winter to 1.06 in summer. Regression analyzes were effective in determining the ETc/ETm ratio, with better performance at high evaporative demand.

Protective role of jaboticaba Plinia peruviana peel extract in copper-induced cytotoxicity in Allium cepa.

Jaboticaba Plinia peruviana (Poir.) Govaerts is a Brazilian berry that presents high levels of polyphenols, which may play a key role in preventing cytotoxic and genotoxic effects of harmful agents. Although copper is an essential micronutrient that plays an important role in organisms, high copper concentrations may trigger toxicity to animals and plants. Here, we investigated whether Plinia peruviana hydroalcoholic extract prevents copper-induced cytotoxicity in Allium cepa root cells. Five different anthocyanins and phenolic compounds were identified in Plinia peruviana extract. Importantly, the exposure to 1.53mg/L copper for 24h impaired mitotic index, as well as increased mitosis disturbances and triggered DNA damage. Pre-incubation with Plinia peruviana extract (0.25g/L and 0.75g/L) for 3h prevented copper-induced changes in the mitotic index and reduced the number of abnormal cells. In conclusion, we suggest that Plinia peruviana peel extract has protective effects against cellular and genetic disturbances induced by copper.

WOOD AND CHARCOAL ANATOMY OF FOUR MYRTACEAE SPECIES

ABSTRACT Qualitative and quantitative changes in anatomical characteristics of charcoal from Campomanesia xanthocarpa, Eugenia pyriformis, Myrcia retorta and Plinia peruviana were described to provide its correct discrimination and information’s to contribute to evaluations in illegal logging. The material came from Planalto Catarinense region, southern Brazil. For each species, three discs at diameter at breast height were collected from three randomly selected trees. All the disks were sectioned to obtain samples in different positions of the trunk (bark, intermediate and pith). Samples were wrapped in aluminum foil and carbonized in a muffle furnace, with a final temperature of 450 °C and a heating rate of 1.66 °C.min-1. In species analyzed, growth rings are few distinct, delimitated by fiber regions; solitary vessel and axial parenchyma diffuse or diffuse-in-aggregate is predominant. The wood to charcoal conversion caused variation in the following characteristics: vessel diameters decreased varying from 21.1% in Myrcia retorta to 33.4% in Eugenia pyriformis, while the vessel frequency increased with values from 50.7% to 92.3% related to the same species; height and width of the rays showed reduction, however, Myrcia retorta presented ruptures in ray cells and dimensions increased; ray frequency increased from 3.9% in Eugenia pyriformis to 54.1% in Campomanesia xantocharpa. Qualitative characteristics of wood from studied species remained in charcoal after carbonization at 450 oC, being possible to discriminate the material based on wood anatomy. In charcoal, vessels dimensions and frequency, after ray characteristics can be applied as first step in this Myrtaceae species distinction at forest control.

Jaboticaba (Plinia peruviana) extract nanoemulsions: development, stability, and in vitro antioxidant activity

Objective: The aim of this work is to develop and characterize nanoemulsions containing jaboticaba extract (Plinia peruviana) aiming pharmaceutical and cosmetic applications.Methods: Nanoemulsions were prepared by high-pressure homogenization method using different concentrations of components (oil, surfactant, and extract) and homogenization pressures, in order to optimize the preparation conditions. Both unloaded and extract-loaded nanoemulsions were characterized according to their size, polydispersity, zeta potential, pH, morphology, and physical stability. Total phenolic and flavonoid contents in free jaboticaba extract and jaboticaba-loaded nanoemulsions were determined spectrophotometrically, while ellagic acid content was determined by high-performance liquid chromatography (HPLC) analysis. In vitro antioxidant activity was investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods.Results: Colloidal dispersions exhibited a mean particle size around 200 nm, with monodisperse size distribution (PdI <0.3), and spherical shape. Stability studies showed that nanoemulsions were stable over 120 d of storage at room temperature. Jaboticaba nanoemulsions showed significant concentrations of phenolics, flavonoids, and ellagic acid, with encapsulation efficiency values higher than 90%. Antioxidant properties of jaboticaba nanoemulsions were demonstrated by its remarkable ability to scavenge DPPH free radicals and to reduce ferric–tripyridyltriazine complex, which can be attributed to their phenolic and flavonoid contents.Conclusions: The results suggest that nanoemulsions containing jaboticaba extract can be considered a promising candidate as a new antioxidant agent.

In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.

Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.

Wild Edible Plants Used by the Polish Community in Misiones, Argentina

We studied the cultural significance of wild edible plants for Eastern European migrants who settled in rural subtropical areas of South America. In 50 interviews with Polish migrants and their descendants in northern Misiones, Argentina, we recorded the use of 41 botanical species and two mushroom taxa. Different cultural significance indices were applied and sociodemographic factors such as gender, age and origin were addressed. Out of the ten most salient species, nine were fruits (Eugenia uniflora, Eugenia involucrata, Rollinia salicifolia, Campomanesia xanthocarpa, Syagrus romanzoffiana, Allophylus edulis, Plinia peruviana, Plinia rivularis, Eugenia pyriformis) and only one was a green vegetable (Hypochaeris chillensis). None of our informants reported famine foods, recreational teas or condiments. Men mentioned more wild edible species than women due to their more extensive knowledge of the forest plants growing further from settlements.