Platelet-rich Plasma In Patients Research Articles

Surgical treatment of acetabular fractures

Background. Acetabular fractures are severe intra-articular injuries that require anatomical reposition and early function, but they are often complicated by degenerative changes in both the acetabulum and the femoral head, leading to the development of post-traumatic coxarthrosis. The purpose was to study the clinical effectiveness of surgical treatment and postoperative management of patients using autologous platelet-rich plasma in patients with fractures of the acetabulum. Materials and methods. Twenty-eight patients with acetabular fractures of various degrees were operated at the City Emergency Hospital of Zaporizhzhia and the Municipal Clinical Hospital No 9 from 2017 to 2019. Gender composition: 22 men (78.57 %), 6 women (21.43 %), average age 46.64 ± 2.21 years, with a 95% confidence interval 42.31–50.96. Nineteen victims (67.86 %) had road traffic injuries, 7 (25 %) domestic injuries, and 2 (7.14 %) had industrial injuries. Results. In the main group of patients treated with platelet-rich plasma, radiologically visible adhesion of the injured area after 8 weeks occurred in 10 people (83.4 %). In one person (8.3 %), the adhesion occurred after 12, and in another (8.3 %) — 16 weeks after surgery. In the control group, adhesions at 8th week were registered in 14 patients (60.87 %), in 8 (34.78 %) — at 16th week. Given the general recovery of the body in the main group, the activation and social adaptation of patients occurred 2–3 weeks earlier. The assessment was performed radiologically and by the criteria of functional recovery.

STUDY OF PLATELET FUNCTION DEFECTS IN CASES OF CHRONIC MYELOID LEUKAEMIA

AIM: Chronic myeloid leukaemia (CML) is one of the chronic myeloproliferative disorders. The emerging data overwhelmingly suggests that study of platelet defects in CML would be useful for comprehensive management of CML. This study was conducted to identify the platelet function defects and to correlate the results with clinical symptoms in cases of CML. METHODS: Clinical and laboratory data of CMLpatients, diagnosed and managed at a tertiary centre from January 2018 to September 2019 were analyzed. Patient's platelet rich plasma was subjected to platelet function tests (PFT) with Chronolog Dual Channel Platelet Aggregometer using arachidonic acid, epinephrine, adenosine diphosphate (ADP) and ristocetin as agonists. RESULTS: Most cases of CML(30%) were from age range of 51 to 60 years and 83% of cases were male. Splenomegaly was seen in 97% of cases. 3 Median haemoglobin was 13 g/dl and median total WBC count was 6.315 x 10 /µL. About 64% of all cases showed the total platelet count of 201- 3 300 x 10 /µL. Median platelet distribution width (PDW) was 10.7 and median value of mean platelet volume (MPV) was 9.4. There was signicant association between platelet aggregation response with ADP and bleeding manifestations. In 98% of cases showing normal platelet aggregation responses with ADP, bleeding manifestation were absent. CONCLUSION: The present study suggests that platelet function defects are seen in CML. Platelet aggregation study can be useful especially in those patients exhibiting bleeding manifestations with normal or increased platelet counts. This would enable a comprehensive management in cases of CML.

Efficiency of use of bone augmentation materials based on multipotent mesenchymal stromal cells of adipose tissue before dental implantation in patients of study groups

Objective. To determine the effectiveness of bone augmentation materials based on multipotent mesenchymal stromal cells of adipose tissue, which were used to fill bone defects during dental operations before dental implantation in patients of the study groups.Materials and methods. Examination and treatment with dental implants were performed on 140 patients who had previously undergone surgery to increase the bone volume of the alveolar processes of the jaws with the use of various bone augmentation materials. All patients were divided into three groups: I (main) group – 49 people (35.0%), in whom the material “Colapan–L” was used to restore the bone tissue of the alveolar processes; II (main) group – 62 patients (44.29%), in whom the augmentation of bone defects was performed using our proposed composition: the drug “Colapan–L”, multipotent mesenchymal stromal cells of adipose tissue and platelet-rich plasma; group III (control) – 29 patients (20.71%) in whom wound healing occurred under a blood clot (spontaneous).Results. It was found that in patients in whom a combination based on multipotent mesenchymal stromal cells of adipose tissue, the drug “Colapan–L”and platelet-rich plasma was used to fill bone defects, peri-implant tissues were completely restored after dental implantation.Conclusions. The effectiveness of our proposed osteoplastic combination based on multipotent mesenchymal stromal cells of adipose tissue, the drug “Colapan–L” and platelet-rich plasma in patients was confirmed by the positive dynamics of the periodontal index PMA, Schiller–Pissarev test. The use of bone augmentation materials based on multipotent mesenchymal stromal cells of adipose tissue during dental operations improves the regenerative and reparative properties of the bone tissue of the jaws, facilitates the postoperative course and reduces the duration of inpatient treatment of patients.

Immunomorphological features of women's skin with androgenetic alopecia in the treatment of plasma enriched with platelets in combination with topical minoxidil 2 % lotion

Introduction. The skin biopsies of 30 patients with androgenetic alopecia before and after treatment with platelet-rich plasma in combination with 2 % minoxidil were examined by immunohistochemistry. Goal. The study looked at immunohistochemical changes in the scalp biopsies of patients with stage I-II androgenetic alopecia according to the Ludwig scale and to identify possible changes after injection of platelet-rich plasma in combination with topical 2 % minoxidil lotion. Materials and methods. Skin biopsies of 30 patients with androgenetic alopecia were examined using an immunohistochemical study before and after treatment, which lasted 3 months and included 3 sessions of PRP (once per month) and topical application of minoxidil 2 % lotion twice a day. Results. It was found that androgenetic alorecia is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of germ polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of collagen IV. Injections of platelet-rich plasma in combination with topical administration of 2 % minoxidil as a treatment for androgenetic alopecia lead to normalization of immunohistochemical parameters of the skin, which indicates the possibility of using this combination for long-term therapeutic effect. Conclusions. This study complemented the understanding of the pathogenesis of AGA and serves as a basis for improving treatment regimens for this pathology. However, additional studies are needed to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.

Immunomorphological features of female patient’s skin with androgenetic alopecia in the treatment of platelet-rich plasma in combination with topical minoxidil 2 % lotion

The aim. The research was provided to obtain immunohistochemical changes in scalp biopsies of female patients with androgenetic alopecia (AGA) I-II stages according to the Ludwig scale and to detect possible changes after usage of platelet-rich plasma (PRP) injections in combination with topical minoxidil 2 % lotion.
 Materials and methods. Skin biopsies of 30 patients with AGA were examined by immunohistochemistry before and after treatment, which lasted for 3 months and included 3 PRP sessions (once per month) and topical application of minoxidil 2 % lotion twice daily.
 Results. It was found that AGA is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of growth polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of Collagen IV. The use of platelet-rich plasma in combination with topical 2 % minoxidil as AGA therapy leads to the normalization of immunohistochemical parameters of the skin, which indicates the possibility of its use for long-term therapeutic effect.
 Conclusions. This study supplemented the understanding of the pathogenesis of AGA and serves as the basis for improving treatment regimens for this pathology. But more research is required to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.

MP56-10 COMPARATIVE ANALYSIS OF THE RESULTS OF TREATMENT OF PATIENTS WITH RECURRENT URETHRAL STRICTURE USING PLATELET-RICH PLASMA

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Urethral Reconstruction (including Stricture, Diverticulum) III (MP56)1 Sep 2021MP56-10 COMPARATIVE ANALYSIS OF THE RESULTS OF TREATMENT OF PATIENTS WITH RECURRENT URETHRAL STRICTURE USING PLATELET-RICH PLASMA Sergey Kotov, and Mikhail Iritsyan Sergey KotovSergey Kotov More articles by this author , and Mikhail IritsyanMikhail Iritsyan More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002086.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Treatment of patients with recurrent urethral stricture requires not only the improvement of surgical techniques, but also the influence on the pathogenetic mechanisms of the formation of urethral stricture. We have previously demonstrated that platelet-rich plasma (PRP) leads to stimulation of regeneration and organization of the intercellular matrix in an experimental model of urethroplasty. To evaluate the clinical effectiveness of reconstructive plastic surgery using platelet-rich plasma in patients with recurrent urethral stricture. METHODS: A comparative analysis of the results of surgical treatment of patients with recurrent urethral stricture with and without the use of platelet-rich plasma was carried out at the University Clinic of Urology of the Pirogov Russian National Research Medical University. A total of 60 patients were included in the study. The main group consisted of 30 patients who were injected with PRP into the spongy body in the operation area. In the control group (30 patients), phys. solution was entered in the same volumes. According to the medians of length and age, localization and etiology, urethral strictures were comparable in the groups. The median maximum urination rate before surgery was 4.7 ml /s (1,7 - 11). According to etiological factors there were iatrogenic strictures in 45 (75%) cases, traumatic – 7 (11.7%), infectious – 2 (3.3%), posthypospadial - 6 (10%). The urethral catheter was removed after surgery in the absence of a contrast agent leak during urethrography. RESULTS: In the main group: anastomotic urethroplasty – 17 patients, augmentation urethroplasty - 9, multi - stage urethroplasty/perineostomy-4. The following operations were performed in the control group: anastomotic urethroplasty in 24 cases, augmentation urethroplasty-in 4 cases, с in 2 cases. The median (min-max) follow – up after surgery was 12 years (6-22 months). The effectiveness in the main group was 93.3%. In 2 cases, a relapse was noted. In the control group, the efficiency was 76.7%. Relapse occurred in 7 cases. The median period of bladder drainage with a urethral catheter after surgery was 14 and 7 days in the control and main groups, respectively (p< 0, 05). The frequency of infectious complications (urethritis, orchepididymitis, p/o wound suppuration) was significantly lower in the main group (p<0.05). The median postoperative Qmax in the control group at the time of observation was (min-max) 19.85 ml/sec (9-23.8), in the main group – 24 ml/sec (10-40) (p>0.05). CONCLUSIONS: The results of urethroplasty with the combined use of platelet-rich plasma in patients with recurrent urethral stricture demonstrate a decrease in the frequency of early relapses of urethral stricture, a reduction in the duration of bladder drainage and the frequency of infectious complications. Source of Funding: No source of funding. © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e972-e973 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sergey Kotov More articles by this author Mikhail Iritsyan More articles by this author Expand All Advertisement Loading ...

Clinical therapeutic effects of platelet-rich plasma in patients with burn wound healing: A protocol for systematic review and meta-analysis.

Background:In clinical settings, burn wounds are frequently encountered. Since burn wounds are a form of physical injury, they can have long-term adverse effects on the human body. It has been a significant challenge to treat burn wounds completely. Since traditional treatment strategies have been unable to heal burn wounds completely, they lack the efficacy to cure the wounds without long-term effects, such as heavy scarring. Reportedly, platelet-rich plasma (PRP) has shown potential to accelerate wound healing. Yet, there are no conclusive reports on a methodological comparative study of research that has assessed the medical benefits of PRP for treating individuals carrying burn wounds. Thus, the present meta-analysis and systematic study aims to assess the medical benefits of PRP for treating patients carrying burn wounds.Methods:The authors will conduct a comprehensive search for randomized controlled trials that evaluate the safeness and efficiency of PRP to treat burn wounds. The search includes 3 Chinese language databases (WanFang database, Chinese BioMedical Literature database, and China National Knowledge Infrastructure) and 4 English language databases (Cochrane Library, EMBASE, Web of Science, and MEDLINE). These electronic databases will be searched from their establishment till May 2021. A pair of independent authors will be selecting eligible studies for extracting data. The same authors will employ the Cochrane risk of bias tool to evaluate the bias risk. We will make use of RevMan (version: 5.3) software to complete data synthesis.Results:The present protocol will establish practical and targeted results evaluating the efficacy and safeness of using PRP to treat burn wounds. The current study also provides a reference for clinical use of PRP.Conclusion:Stronger evidence about the effectiveness and safety of using PRP to treat and heal burn wounds will be provided for clinicians to refer.Ethics and dissemination:Ethics approval is unrequired.Registration number:March 31, 2021.osf.io/whauj. (https://osf.io/whauj/).

Comparison of the efficacy of low-intensity shock wave therapy and its combination with platelet-rich plasma in patients with erectile dysfunction.

We aimed to compare the efficacy of low-intensity shock wave therapy (Li-SWT) alone and its combination with platelet-rich plasma (PRP) in the treatment of patients with erectile dysfunction (ED). Between January 2015 and October 2020, patients who did not benefit from the use of phosphodiesterase type 5 inhibitors (PDE5i; 5mg/day) for at least 3months and underwent Li-SWT or Li-SWT with PRP were evaluated retrospectively. There were 93 patients who were subjected to Li-SWT only (Group 1) and 91 patients subjected to Li-SWT with PRP (Group 2). Analysis of the International Index of Erectile Function-Erectile Function Area (IIEF-EF) scores showed a significant increase in both the groups post-treatment (Group 1: from 14.33±4.39 to 23.8±4.37, p=.001; Group 2: from 17.82±3.44 to 26.3±2.55, p=.001). When the increase in the IIEF-EF scores was compared pre- and post-treatment between the groups with respect to the ED grades, there was no statistically significant difference between them. Furthermore, while the intravaginal ejaculatory latency time (IELT) in successfully treated patients of Group 1 remained the same, Group 2 presented 1.5-3.5 times (mean, 2.4) prolongation. Their mean IELT score showed an increase from 2.2 (0.8-3.5)min to 5.3 (2.8-10.5)min. Our study shows that combination treatment of Li-SWT with PRP injections is not only safe for patients with ED, but also effective and safe in prolonging the IELT.

Infiltração intraarticular de plasma rico em plaquetas versus ácido hialurônico em pacientes com osteoartrose primária do joelho: Ensaio clínico randomizado com resultados preliminares

Objective The present study aimed to compare the effects of intraarticular infiltration of platelet-rich plasma with those of hyaluronic acid infiltration in the treatment of patients with primary knee osteoarthritis. Methods A randomized clinical trial was conducted with 29 patients who received an intraarticular infiltration with hyaluronic acid (control group) or platelet-rich plasma. Clinical outcomes were assessed using the visual analog scale for pain and the Western Ontario and McMaster Universities Arthritis Index (WOMAC) questionnaire before and after the intervention. In addition, the posttreatment adverse effects were recorded. Categorical variables were analyzed using the chi-square and Fisher exact tests, whereas continuous variables were analyzed using the Student t test, analysis of variance, and the Wilcoxon test; all calculations were performed with the Stats package of the R software. Results An independent analysis of each group revealed a statistical difference within the first months, with improvement in the pain and function scores, but worsening on the 6 th month after the procedure. There was no difference in the outcomes between the groups receiving hyaluronic acid or platelet-rich plasma. There was no serious adverse effect or allergic reaction during the entire follow-up period. Conclusion Intraarticular infiltration with hyaluronic acid or platelet-rich plasma in patients with primary knee gonarthrosis resulted in temporary improvement of functional symptoms and pain. There was no difference between interventions.

A Systematic Review Evaluating the Efficacy of Intra-Ovarian Infusion of Autologous Platelet-Rich Plasma in Patients With Poor Ovarian Reserve or Ovarian Insufficiency.

The emergence of autologous platelet-rich plasma (PRP) therapy reflects a break-through for infertile patients with premature ovarian failure. To study the efficacy of intra-ovarian infusion of autologous PRP on the improvement of ovarian reserve parameters and the subsequent artificial reproductive technique (ART) cycle outcomes in infertile women with poor ovarian reserve or premature ovarian insufficiency, a systematic search in electronic databases like Medline (through PubMed), Embase, Scopus, Web of Science, and Cochrane was done using relevant search terms. Except for case series, case reports, and review articles, all other types of studies, those evaluated for the effects of intra-ovarian infusion of PRP in subfertile women for decreased ovarian reserve (DOR) or premature ovarian insufficiency (POI) were included in our systematic review. The data were extracted from each eligible study and cross-checked by two authors. Intra-ovarian PRP infusion appears to be effective in ovarian rejuvenation, and the results of the subsequent intracytoplasmic sperm injection (ICSI) cycle are encouraging. PRP intervention was found to be beneficial in terms of an improvement in ovarian reserve parameters (increase in serum anti-mullerian hormone or antral follicle count or decrease in serum follicular stimulating hormone). ICSI cycle performance in terms of the total number of oocytes retrieved, number of two-pronuclei embryos, fertilization rate, number of cleavage stage embryos, number of good quality embryos, and cycle cancellation rate were found to be improved after intra-ovarian PRP infusion as compared to their previous cycle without PRP infusion.

Platelet-Rich Plasma in Patients With Partial-Thickness Rotator Cuff Tears or Tendinopathy Leads to Significantly Improved Short-Term Pain Relief and Function Compared With Corticosteroid Injection: A Double-Blind Randomized Controlled Trial

To perform a randomized controlled trial comparing platelet-rich plasma (PRP) with standard corticosteroid (CS) injection in providing pain relief and improved function in patients with rotator cuff tendinopathy and partial-thickness rotator cuff tears (PTRCTs). This double-blind randomized controlled trial enrolled patients with ultrasound-proven or magnetic resonance imaging-proven PTRCTs who received either an ultrasound-guided PRP or CS injection. Patients completed patient-reported outcome assessments at baseline and at 6 weeks, 3 months, and 12 months after injection. The primary outcome was improvement in the visual analog scale (VAS) score for pain. Secondary outcomes included changes in American Shoulder and Elbow Surgeons (ASES) and Western Ontario Rotator Cuff Index (WORC) scores. Treatment failure was defined as subsequent injection, consent to undergo surgery, or operative intervention. We followed up 99 patients (47 in the PRP group and 52 in the CS group) until 12 months after injection. There were no differences in baseline patient demographic characteristics including age, sex, or duration of symptoms. Despite randomization, patients in the PRP group had worse baseline VAS (46.0 vs 34.7, P= .01), ASES (53.9 vs 61.8, P= .02), and WORC (42.2 vs 49.5, P= .03) scores. At 3 months after injection, the PRP group had superior improvement in VAS (-13.6 vs 0.4, P= .03), ASES (13.0 vs 2.9, P= .02), and WORC (16.8 vs 5.8, P= .03) scores. There were no differences in patient-reported outcomes at 6 weeks or 12 months. There was no difference in the rate of failure (P= .31) or conversion to surgery (P= .83) between groups. Patients with PTRCTs or tendinopathy experienced clinical improvement in pain and patient-reported outcome scores after both ultrasound-guided CS and PRPinjections. Patients who received PRP obtained superior improvement in pain and function at short-term follow-up (3months). There was no sustained benefit of PRP over CS at longer-term follow-up (12 months). Level I, randomized controlled trial.

The effect of platelet-rich plasma in patients with early hip osteoarthritis: a pilot study

Abstract We investigate whether platelet-rich plasma (PRP) injections can improve symptoms and function in patients with mild to moderate osteoarthritis (OA). Data were prospectively collected and retrospectively reviewed for all patients receiving PRP intra-articular hip injections between February 2017 and June 2017. The inclusion criteria were patients with a well-preserved joint space (Tönnis 0 or 1) whose magnetic resonance imaging (MRI) findings demonstrated degenerative joint disease or a Tönnis grade of 2. The patient-reported outcomes (PROs) used were the modified Harris Hip Score (mHHS), Hip Outcome Score-Activities of Daily Living Subscale (HOS-ADL), Hip Outcome Score-Sports Specific Subscale (HOS-SSS), International Hip Outcome TOOL (iHOT-12), Single Assessment Numeric Evaluation (SANE) and Mental and Physical aspects of the Veteran RAND 12 Item Health Survey (VR-12M and VR-12P). The visual analog scale (VAS) was utilized to indicate pain. Nine patients (11 hips) were eligible for inclusion. All PROs and VAS improved from pre- to post-injection. These improvements were present at the 3-month follow-up visit and stable until the 12-month follow-up. There was statistically significant improvement for mHHS (P &amp;lt; 0.001), HOS-ADL (P = 0.006), iHOT-12 (P = 0.003) and VR-12M (P = 0.005) at 12 months post-injection. Similarly, VAS improved from 4.1 to 2.3, although the change was not statistically significant. PRP injections significantly improved PROs in all measured scales at time points up to a year after intervention, except for VR-12P and HOS-SSS. In conclusion, patients with early OA of the hip had significant improvement of patient-reported functional outcomes up to 12 months after PRP intra-articular injections.

Применение гранулоцитарного колониестимулирующего фактора и плазмы, обогащенной тромбоцитами, в подготовке пациенток с «тонким» эндометрием в программах переноса размороженных эмбрионов

Применение гранулоцитарного колониестимулирующего фактора и плазмы, обогащенной тромбоцитами, в подготовке пациенток с «тонким» эндометрием в программах переноса размороженных эмбрионов

TGF-β1 level in platelet-rich plasma in patients with diseases and injuries of the musculoskeletal system

TGF-β1 level in platelet-rich plasma in patients with diseases and injuries of the musculoskeletal system

SC194 - Intravesical application of Platelet-Rich Plasma in patients with persistent haemorrhagic cystitis after hematopoietic stem cell transplantation: A single-centre preliminary experience

SC194 - Intravesical application of Platelet-Rich Plasma in patients with persistent haemorrhagic cystitis after hematopoietic stem cell transplantation: A single-centre preliminary experience

Intravesical application of platelet-rich plasma in patients with persistent haemorrhagic cystitis after hematopoietic stem cell transplantation: a single-centre preliminary experience.

Haemorrhagic cystitis (HC) after allogeneic transplantation (HSCT) is a condition characterized by diffuse inflammation and bleeding from the bladder mucosa. Treatment of HC is not standardized and clinical Guidelines are elusive. The aim of this study was to evaluate the safety and efficacy of intravesical treatment with platelet-rich plasma (PRP) in patients with HC after allogenic HSCT. Data from ten consecutive patients with BK virus-induced HC between 2013 and 2017 were collected. HC was classified into four grades. Inclusion criteria were (a) grade 3 or 4 BKV-induced HC after allogenic HSCT; (b) HC refractory to conservative therapy. All patients underwent transurethral cystoscopy and PRP treatment under general anaesthesia. Mean patients' age was 33.6years. Four patients (40%) presented a grade 3 BKV-induced HC and six patients (60%) a grade 4. No intraoperative complications occurred. Postoperative complications were recorded in six patients: three patients required blood transfusion while three patients endovenous antibiotic therapy. Median time to catheter removal was 6days (IQR 2-10). Median length of hospitalization was 35days (IQR 6-73). At 30days after surgery, a three-way catheter was repositioned in one patient for grade 4 haematuria, six patients had a complete response, and three a partial response. Our preliminary experience suggests that intravesical administration of PRP should be considered as a feasible and safe option for the treatment of BK-induced HC after HSCT. Future studies are needed to assess its potential value in other forms of haemorrhagic cystitis.

AUTOLOGOUS PLATELET‐RICH PLASMA: A REVIEW OF SCIENTIFIC ARTICLES ON THE STUDY OF EFFICIENCY IN TREATMENT OF ANDROGENETIC ALOPECIA IN MEN AND WOMEN

This article is an analysis of scientific publications that reflect the experience of using autologous plateletrich plasma in patients with androgenic alopecia (AGA), a multifactorial genetically predisposed disease caused by the influence of exogenous or endogenous triggers responsible for the clinical manifestations of pathology. Attention to this topic is due to the significant prevalence of AGA, as well as unsatisfactory results in achieving a positive therapeutic effect. Positive results were obtained during the 12 clinical studies among the conducted ones, no positive dynamics were observed in the end of other 2 studies. It can be noted that different methods and parameters of centrifugation were used to perform these experiments: PRP used differ or PRP activators were not used at all, variations were observed in methods for evaluating the obtained results, the multiplicity of procedures and intervals between them, the procedure technique (using a mezoroller, anesthetics, injections, etc.). Studies with a negative result were characterized by the smallest number of procedures performed (1 or 2 procedures), and methods for evaluating the results cannot be called sufficiently reliable (a magnifying glass). It is obvious that the use of PRP has a positive effect on the state of AGA and can be used with approved methods of this disease treatment. However, the published results of only a small number of clinical trials on the effectiveness of PRP for hair growth cannot be considered objective. In addition, there is no published agreed protocol for the standard use of PRP, and the use of growth factors and stimulants in the expression of their genes still requires comparative evaluation with preparations approved for the treatment of hair. Thus, the method of PRP using can be considered as a method of treating AGA, however, it is necessary to continue research for further study and standardization of this technique.

Effectiveness of Intra Articular Injection of Platelet-Rich Plasma in Patients with Anterior Disc Displacement with Reduction

Background: Anterior disc displacement with reduction (ADDWR) is the most common form of the internal derangement (ID) of temporomandibular joint (TMJ). It is a painful progressive dysfunction and clinically characterized by reciprocal clicking due to shift in the disc anteriorly in relation to the condyle and fossa during mandible elevation. Minimally invasive therapy such as intra-articular injection of platelet-rich plasma (PRP) has been used. PRP is a natural autologous product with a high platelet concentration obtained by centrifugation process to enhance tissue healing through several growth factors (GFs), which are released after endogenous activation. The aim of this study is to assess this technique which is increasingly used today as a safe, simple modality. Materials and Methods: sixty patients with ADDWR were participated (Females 41, males 19; age 20-39 years). Patients were divided into two groups; PRP was used as study group and conservative therapy was used as control group. 1 ml of PRP was obtained by a single step centrifugation. Pain, joint sounds and maximum mouth opening were clinically evaluated before and two months after therapy. Results: There was statistically significant improvement in pain score, clicking and maximum mouth opening in patients treated with PRP. No complications were recorded immediately and two months after therapy. Conclusion: This study support the effectiveness of PRP injection in treatment of ADDWR as a safe modality in reducing pain, clicking and increase mouth opening.

Safety and Efficacy of Intra-articular Injection of Platelet-Rich Plasma in Patients With Ankle Osteoarthritis.

An intra-articular injection of platelet-rich plasma (PRP) may be an effective treatment for osteoarthritis (OA). However, its efficacy in ankle OA has not been investigated yet. The purpose of this study was to assess the safety and efficacy of an intra-articular injection of PRP in patients with ankle OA during a 24-week period. Twenty ankles of 20 patients with varus-type ankle OA who received intra-articular injections of PRP were evaluated. PRP was extracted from whole blood by using the double-spin technique. Three injections of 2-mL PRP were administered to the ankle at an interval of 2 weeks under ultrasonographic guidance. Adverse events and efficacy were assessed at 4, 12, and 24 weeks after the last injection. Clinical outcomes were assessed by using the visual analog scale (VAS) for pain, the Japanese Society for Surgery of the Foot (JSSF) ankle/hindfoot scale, and the Self-Administered Foot Evaluation Questionnaire (SAFE-Q). No serious adverse effects were observed during the follow-up period. The VAS and JSSF scale scores significantly decreased from baseline to 4, 12, and 24 weeks after treatment ( P < .001). The mean score in the pain-related subscale of the SAFE-Q significantly improved from baseline to 12 weeks after treatment ( P = .04). Overall, the amount of pain reduction was maximal at 12 weeks after the last injection, and the effect was reduced at 24 weeks. The patients with late-stage OA had worse scores in all outcomes than those with early-stage OA. Intra-articular injections of PRP resulted in no serious adverse effects and significantly reduced pain in the patients with ankle OA. PRP treatment can be safe and effective and may be an option in the treatment of ankle OA. Level IV, case series.

Prospects for the use of autologous platelet-rich plasma in patients with recurrent implantation failure

Prospects for the use of autologous platelet-rich plasma in patients with recurrent implantation failure