Abstract
The aim. The research was provided to obtain immunohistochemical changes in scalp biopsies of female patients with androgenetic alopecia (AGA) I-II stages according to the Ludwig scale and to detect possible changes after usage of platelet-rich plasma (PRP) injections in combination with topical minoxidil 2 % lotion.
 Materials and methods. Skin biopsies of 30 patients with AGA were examined by immunohistochemistry before and after treatment, which lasted for 3 months and included 3 PRP sessions (once per month) and topical application of minoxidil 2 % lotion twice daily.
 Results. It was found that AGA is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of growth polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of Collagen IV. The use of platelet-rich plasma in combination with topical 2 % minoxidil as AGA therapy leads to the normalization of immunohistochemical parameters of the skin, which indicates the possibility of its use for long-term therapeutic effect.
 Conclusions. This study supplemented the understanding of the pathogenesis of AGA and serves as the basis for improving treatment regimens for this pathology. But more research is required to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.
Highlights
IMMUNOMORPHOLOGICAL FEATURES OF FE-The material for the studyResearch has opened up a huge potential for developing ways to correct and manage the hair growth cycle with biologically active substances, growthMALE PATIENT’S SKIN WITH ANDROGENETIC ALOPECIA IN THE TREATMENT OF PLATELET-RICH PLASMA IN COMBINATION WITH TOPICAL MINOXIDIL 2 % LOTION were scalp biopsies of 30 women with androgenetic alopecia (AGA) aged 22 to 40 years who were monitored in the period from 2017 to 2020 at the clinical base factors and hormones, and has revolutionized this
Between the preserved hair follicles, in the area of empty hair bags in the area of the upper part of the hair follicles, areas of dermal fibrosis were found, they consisted of concentrically located bundles of connective tissue fibers
Fibroblasts, epitheliocytes, endothelial cells of its capillary in the inflammatory process, which we found in 93.3 % of observations of the main study group, as well as T-lymphocytes and macrophages of inf lammatory infiltrate lose the ability to fully produce these control substances
Summary
MALE PATIENT’S SKIN WITH ANDROGENETIC ALOPECIA IN THE TREATMENT OF PLATELET-RICH PLASMA IN COMBINATION WITH TOPICAL MINOXIDIL 2 % LOTION were scalp biopsies of 30 women with AGA aged 22 to 40 years (mean age 32.1 years) who were monitored in the period from 2017 to 2020 at the clinical base factors and hormones, and has revolutionized this. Olena Salyenkova Department of Infectious Diseases and Clinical Immunology. The research was provided to obtain immunohistochemical changes in scalp biopsies of female patients with androgenetic alopecia (AGA) I-II stages according to the Ludwig scale and to detect possible changes after usage of platelet-rich plasma (PRP) injections in comof the Department of Infectious Diseases and Clinical Immunology of the Medical Faculty of V. Patients received injections of platelet-rich plasma in combination with topical minoxidil 2 % lotion
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