Determination of antimicrobial and fungicidal properties of experimental disinfectant "Sukhodez"

Tetiana Fotina,Oleksii Fotin,Dasha Slasten

doi:10.21303/2585-6634.2021.002124

Tetiana Fotina, Oleksii Fotin

Open Access

https://doi.org/10.21303/2585-6634.2021.002124

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Abstract

The results of the study of antimicrobial and fungicidal properties of the experimental preparation “Sukhodez” against microorganisms E. coli, Salmonella enteritidis, Staphylococcus aureus and fungi Candida albicans in the laboratory are presented. The aim of the research. To study the antimicrobial and fungicidal properties of powdered disinfectant and analyze the prospects for its further use in a set of anti-epizootic measures in particular in poultry farms. Materials and methods. The research was conducted during 2021 at the Department of Veterinary Examination, Microbiology, Zoohygiene and Safety and Quality of Animal Products of the Faculty of Veterinary Medicine of Sumy National Agrarian University. Evaluation of bactericidal properties of the experimental agent “Sukhodez” was determined on museum strains of E. coli ATCC 25922, Salmonella enteritidis ATCC 13076 and Staphylococcus aureus ATCC 6538, fungicidal properties were studied on fungi Candida albicans. All cultures were standardized to 109 CFU / cm3. Results. It is established that as a result of the conducted researches at studying preparation “Sukhodez” antimicrobic properties, it had high efficiency concerning action on strains of both gram-positive and gram-negative bacteria. It had a detrimental effect on bacteria when applied to concrete at an exposure of 1 hour with a rate of 75 mg per 1 m2, and had the same effect as when applied to a wooden surface, where it expressed antimicrobial action at a rate of 50 mg/m2 after 4 hours. When determining the fungicidal properties, it was found that when exposed to 5 hours, the preparation “Sukhodez” had an effect on study contaminated objects with a rate of 50 mg/m2, and with a rate of 75 mg/m2 inhibited the growth of fungi for 1 hour. Conclusions. It was found that the most sensitive to the preparation “Sukhodez” were the culture of Salmonella enteritidis – at a rate of 25 g / m2 and action on concrete and wooden surfaces, the disinfectant inhibited the growth of the culture when exposed to 5 hours, and at a rate of 50 g / m2 – for 2 years. Staphylococcus aureus was the most stable bacterial culture, so at a rate of 50 g / m2 on concrete and wood surfaces, bacterial growth was inhibited for 4 and 5 hours, respectively. At the same time, in the study of fungicidal properties, “Sukhodez” showed a fungicidal effect on Candida albicans when applied to a concrete surface at a rate of 50 mg / m2 for 3 hours, when applied to a wooden surface – for 4 hours. In general, at a rate of 75 g / m2, “Sukhodez” has an instant bactericidal and fungicidal effect on bacteria and fungi applied to concrete and wooden surfaces

Highlights

Poultry farming is one of the most promising and fastest growing branches of business, and poultry is one of the world’s largest stocks of domestic animals [1, 2]
Materials and methods Evaluation of bactericidal properties of the experimental agent “Sukhodez” was determined on museum strains of E. coli ATCC 25922, Salmonella enteritidis ATCC 13076 and Staphylococcus aureus ATCC 6538, fungicidal properties were studied on fungi Candida albicans
When studying the effectiveness of the experimental disinfectant “Sukhodez” on the pathogens of the most common infectious and fungal diseases that were on the test objects in direct contact (Tables 1, 2), it was found that the experimental preparation “Sukhodez” was effective against a controlled spectrum of pathogens of bacterial diseases and had an adsorbing effect

Summary

Introduction

Poultry farming is one of the most promising and fastest growing branches of business, and poultry is one of the world’s largest stocks of domestic animals [1, 2]. Preventive measures, including cleaning and disinfection, are fundamental steps for biosecurity programs and indispensable for maintaining high productivity on poultry and livestock facilities [5]. Spraying disinfectants in barns and removing feces were the only sanitation schemes adopted in farms [8, 9]. These disinfectants are used without regular inspection and evaluation of effectiveness, while the effectiveness of disinfectants is influenced by the composition, level of organic matter, humidity, temperature, dilution rate, pH and hardness of water and other factors [10]. The use of disinfectants without validation and evaluation can lead to high selective pressure, which will gradually reduce the sensitivity of organisms to the disinfectants used and even cross-resistance to antibiotics of health concern.

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