Total Plasma Protein Research Articles

Salinity levels affect the lysine nutrient requirements and nutrient metabolism of juvenile genetically improved farmed tilapia (Oreochromis niloticus).

This 62-d research aimed to evaluate the effects of dietary lysine levels (DLL) and salinity on growth performance and nutrition metabolism of genetically improved farmed tilapia (GIFT) juveniles (Oreochromis niloticus). Six diets with lysine supplementation (1·34, 1·70, 2·03, 2·41, 2·72 and 3·04 % of DM) were formulated under different cultured salinities in a two-factorial design. The results indicated that supplemental lysine improved the specific growth rate (SGR) and weight gain (WG) and decreased the feed conversion ratio (FCR). Meanwhile, the fish had higher SGR and WG and lower FCR at 8 ‰ salinity. Except for moisture, the whole-body protein, lipid and ash content of GIFT were increased by 8 ‰ salinity, which showed that DLL (1·34 %) increased the whole-body fat content and DLL (2·41 %) increased whole-body protein content. Appropriate DLL up-regulated mRNA levels of protein metabolism-related genes such as target of rapamycin, 4EBP-1 and S6 kinase 1. However, 0 ‰ salinity reduced these protein metabolism-related genes mRNA levels, while proper DLL could improve glycolysis and gluconeogenesis mRNA levels but decrease lipogenesis-related genes mRNA levels in liver. 0 ‰ salinity improved GLUT2, glucokinase and G6 Pase mRNA levels; however, sterol regulatory element-binding protein 1 and fatty acid synthase mRNA levels were higher at 8 ‰ salinity. Moreover, 8 ‰ salinity also increased plasma total protein and cholesterol levels and decreased glucose levels. These results indicated that the recommended range of lysine requirement under different salinity was 2·03-2·20 % (0 ‰) and 2·20-2·41 % (8 ‰) and 8 ‰ salinity resulted in higher lysine requirements due to changes in the related nutrient metabolism, which might provide useful information for designing more effective feed formulations for GIFT cultured in different salinity environment.

Selective removal of soluble tumor necrosis factor receptors by apheresis as a novel immunotherapy approach for triple-negative breast cancer.

e13065 Background: Although the activity of the cytokine TNF-α against diverse tumor types has been widely documented, safe and effective therapies for augmenting this pathway are lacking. An immunoadsorption device, the LW-02 Column, has been developed as a subtractive immunotherapy approach to remove soluble TNF-α receptors (sTNF-R1 and sTNF-R2) from cancer patients’ plasma using apheresis. Since soluble TNF-α receptors bind to and neutralize the activity of TNF-α, their depletion from plasma is performed to unleash the anti-tumor effects of endogenous TNF-α. The LW-02 Column contains an inert matrix to which a recombinant TNF-α polypeptide (single-chain TNF-α; scTNF) is covalently coupled as a ligand for capturing sTNF-R1 and sTNF-R2. This study evaluated key performance features of the LW-02 Column when used as a monotherapy or with chemotherapy in patients with advanced TNBC [ClinicalTrials.gov Identifier: NCT04004910]. Methods: As part of the ongoing clinical study, 28 patients with advanced TNBC underwent 862 LW-02 Column Immunopheresis procedures at 3 sites (mean=22.5; range=2–109) using the Spectra Optia Apheresis System (Terumo BCT, Inc.); 10 patients received LW-02 Column monotherapy and 18 received LW-02 Column therapy with various chemotherapy regimens. Therapy with the LW-02 Column was performed 3x/week for at least 16 weeks by processing 2 plasma volumes per treatment. Adverse events and tolerability of the therapy were monitored. Assessments were performed to determine the LW-02 Column’s target specificity and capture efficiency. The amount of scTNF ligand that could leach from the column during the procedures was also measured. Results: No serious device-related adverse events have been reported in this study. After 30 minutes of apheresis using the LW-02 Column, the mean capture efficiencies for sTNF-R1 and sTNF-R2 from TNBC patients’ plasma were 95.2% and 79.6%, respectively. The LW-02 Column was highly selective for removing sTNF-R1 and sTNR-R2 from total plasma proteins. The mean total amount of scTNF that may leach from the LW-02 Column (109 ng per apheresis session) is orders of magnitude lower than TNF-α concentrations that are known to trigger clinically meaningful effects. Conclusions: In patients with advanced TNBC, subtractive immunotherapy with the LW-02 Column either as a monotherapy or with chemotherapy is safe and achieves effective depletion of soluble TNF-α receptors from plasma. Evaluation of the safety and efficacy of the LW-02 Column is ongoing in TNBC patients as well as in studies of other solid tumor types. The LW-02 Column provides a novel and promising immunotherapy approach for augmenting the anti-cancer activity of the TNF-α pathway. Clinical trial information: NCT04004910.

Repeated intraperitoneal injection of ovine growth hormone accelerates growth in sub-yearling Siberian sturgeon Acipenser baerii

The role of growth hormone (GH) in chondrosteans is poorly understood, particularly with regard to its effects on growth. In this study, we examined the influence of exogenous GH on growth performance and body composition in juvenile Siberian sturgeon (Acipenser baerii). Fish with initial weight of 80.2 ± 0.1 g (mean ± S.E) were injected once every 10 days with either purified ovine GH (oGH) at 1, 2, 4, and 8 μg oGH/g body weight (BW) or with saline over a 50-day period. Treatment with the highest dose of oGH significantly enhanced growth performance (final body weight and length, body weight increase and specific growth rate, SGR). Notably, 8 μg oGH/g BW increased body weight by 33% and SGRw by 141% compared to control fish. GH-stimulated (8 μg oGH/g BW) growth was accompanied by increased crude protein content; however, oGH treatment did not affect levels of total protein, total lipid, cholesterol, triglyceride, or glucose in plasma. oGH decreased plasma levels of thyroxine (at 4 μg oGH/g BW), but had no significant effect on plasma levels of triiodothyronine or cortisol compared to controls. These findings indicate that 8 μg oGH/g BW enhances somatic growth and synthesis of body protein in juvenile Siberian sturgeon and demonstrate the feasibility of exogenous oGH treatment in conservation and aquaculture programs for this ancient species.

1460-P: Tauroursodeoxycholic Acid (tudca) Improves the Morphofunction of Pancreatic Beta Cells in Postweaning Protein-Restricted Mice

Protein restriction in the postweaning diet in mice impairs glucose metabolism by reducing insulin secretion from pancreatic β cells. TUDCA in obese rodents and humans normalized insulin secretion, increased insulin clearance, furthermore recovered mass and the number of β cells per islet in animal models of diabetes. This study aimed to evaluate the effects of TUDCA on the morphology and function of pancreatic β cells in mice subjected to postweaning protein restriction. Male C57Bl/6J mice at 30 days of age received a control diet (14% protein - group C) or a protein-restricted diet (6% protein - group R) for 16 weeks. During the last 2 weeks, 50% of the animals in each group received intraperitoneal treatment with daily doses of 300 mg/kg of body weight of TUDCA (CT and RT) , and the remainder received PBS (C and R) . The R mice that showed decreased total plasma protein and serum albumin concentration had lower weight gain and feed efficiency, but increased white adipose tissue weight. There were no differences in fasting blood glucose between the groups. The R mice that have increased insulin sensitivity after treatment with TUDCA had normalized sensitivity. Isolated islets from R mice have lower insulin secretion, which has been associated with decreased morphometric parameters compared to C. In RT mice secretion was restored in response to 2.8 mM and 11.mM glucose and the pancreas of the RT showed a greater area of β cells in relation to the area of the islets and a greater number of islets compared to R. Furthermore, RT mice showed an increase in the expression of genes of the SNARE complex, genes of function, transcription and differentiation of pancreatic β cells. We conclude that TUDCA improves glycemic homeostasis, increases insulin secretion and improves the functioning of pancreatic β cells in mice subjected to protein restriction, indicating that TUDCA can be used as a therapeutic strategy to normalize insulin secretion in protein malnutrition. Disclosure L.M.Barreto dos santos: None. T.Araujo: None. B.L.Alves: None. S.Ferreira: None. P.L.Zimath: None. J.F.Vettorazzi: None. E.M.Carneiro: None. Funding Conselho Nacional de Desenvolvimento Científico e Tecnológico (134270/2019-3)

Anthelmintic efficacy of Copaifera reticulata oleoresin in the control of monogeneans and haematological and histopathological effects on Colossoma macropomum

This study investigated for the first time the anthelmintic efficacy of therapeutic baths with Copaifera reticulata oleoresin and nanoemulsion with this oleoresin against monogeneans of Colossoma macropomum, as well as the haematological and histological effects in this fish. In the therapeutic baths of three consecutives were used 100 mg/L of C. reticulata oleoresin or 250 mg/L of nanoemulsion containing C. reticulata oleoresin. Therapeutic baths with 100 mg/L of C. reticulata oleoresin had anthelmintic an efficacy of 48.5% against monogeneans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri), while baths with nanoemulsion of C. reticulata oleoresin had not anthelmintic efficacy, which was discussed. Baths for 1 h with 100 mg/L of C. reticulata oleoresin or 2 h with 250 mg/L of C. reticulata nanoemulsion increased levels of plasma total protein and glucose, mean corpuscular volume (MCV) and neutrophils number in C. macropomum and decreased in the number of total leucocytes and lymphocytes. In the gills of fish exposed and controls occurred detachment of the epithelium, hyperplasia and hypertrophy, resulting in moderate fusion of the secondary lamellae. Therefore, therapeutic baths with C. reticulata oleoresin and nanoemulsion of C. reticulata oleoresin have a low toxicity to C. macropomum, as there were few changes to the blood parameters.

Housing Effect on Growth, Physiological and Blood Indices in Crossbred Sheep During Winter

Background: The winter chill in temperate zones of the country affects animal production. It is understood that during cold winter animals have to maintain their body temperature through increased heat production, thus energy partitioning towards the production is compromised. Since sheep has a thick wooly coat that insulates the animal from harsh ambient temperatures, hence present study was undertaken to assess the impact of housing during cold winter on basic physiological, hemato-biochemical indices and growth in crossbred sheep in temperate Kashmir. Methods: Twenty crossbred weaners of same age, sex and body weight were selected and divided into two groups of ten animals each. One group of sheep was managed under conventional closed shed and another group was managed under open fenced shed during winter from January to March. All the animals were maintained on a uniform nutritional regime. Physiological, hemato-biochemical parameters, body surface temperature, body weight and stress hormones were measured fortnightly. Result: Body weight of animals was significantly higher in closed shed (21.04±0.23 kg) as compared to open shed (18.20±0.19 kg). Heart rate, respiratory rate, body surface temperature differed significantly among the groups. NLR was significantly higher in animals under open shed (0.70) as compared to closed shed animals (0.63). Significant difference was observed in stress hormone cortisol with higher values in open shed animals (12.44±0.07 ng/ml) than in closed shed animals (9.97±0.08 ng/ml). No significant difference was observed in plasma total protein and A/G ratio, however, glucose was significantly higher in animals of open shed as compared to closed shed. Present study revealed that housing management during winter in sheep was essential to maintain production.

Successful Pregnancy Outcome in Mares: The Potential Role of Body Conditional Score, Age and Biochemical Parameter's Adjustments

The objective of this research was to evaluate the changes in body condition score (BCS), age and the main biochemical parameters in pregnant Spanish Purebred mares. A total of 45 pregnant Spanish broodmares has been studied, classified in three different groups: group A (n=15), aging 14-18 years old and BCS lower than 4 of 10, group B (n=15), with ages of 4 to 8 years and a BCS of 5-6/10, and group C (n=15), aging between 6 and 11 years and a BCS of 8 of 10. Three pregnancy periods were considered: the 1st period, from the 1st to the 3rd, the 2nd from the 4th to the 6th, and the last period, from the 7th month of pregnancy to delivery. Blood samples were taken every month along the pregnancy. The following plasma biochemical parameters were measured: triglycerides, total cholesterol, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, total, direct and indirect bilirubin, aspartate aminotransferase, lactate dehydrogenase, γ-glutamyl transferase, alkaline phosphatase, total plasma proteins, albumin, glucose, urea and creatinine. Pregnancy in the Spanish broodmare induced hyperlipidemia with hypertriglyceridemia and increased plasma concentrations of total, direct and indirect bilirubin (P < .05). Older pregnant mares showed biochemical changes probably consistent with decreased organ functionality (P < .05). Obesity did not influence lipidic metabolism during pregnancy (P > .05).

Effect of dietary chicken gut meal levels on growth performance, plasma biochemical parameters, digestive ability and fillet quality of Cyprinus carpio

An eight-week feeding trial was conducted to investigate the effects of chicken gut meal (CGM) in experimental diet on growth performance, plasma biochemical parameters, digestive enzymes activity, intestinal morphologic, muscle composition, and fillet quality of common carp (Cyprinus carpio) (initial body weight: 57.34 ± 0.13 g). Experimental fish were fed with experimental diets containing CGM at 0% (CGM 0 group), 5% (CGM 5% group), 10% (CGM 10% group), 15% (CGM 15% group), and 100% (CGM 100% group) levels, respectively. The results revealed that CGM significantly improved final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), and feed conversion ratio (FCR) (P < 0.05) of experimental fish. Plasma GH and IGF-I concentrations in the CGM 15% group were substantially higher than in the other groups (P < 0.05). CGM 15% group showed the highest FBW, WG, SGR, and lowest FCR (P < 0.05) of all treatments. Except for plasma total protein (TP), albumin (ALB), and globulin (GLB) contents, the plasma biochemical parameters were significantly improved (P < 0.05). Pepsin (PEP), amylase (AMS), and lipase (LPS) activities were significantly higher in CGM 10%, CGM 15%, and CGM 100% groups compared to the control group and reached a maximum in the CGM 15% group (P < 0.05). Diet supplementation with CGM significantly increased midgut villi height (VH), villi width (VW), and muscle layer thickness (MT) in common carp. The inclusion of dietary CGM to the diet could improve the crude protein and crude lipid content of common carp muscle, as well as influence fillet quality by increasing chewiness. In conclusion, the optimum inclusion of CGM to the diet is 15%, which can considerably improve growth performance, plasma biochemical parameters, digestive activity, and fillet quality of common carp.

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

The present study aimed to identify the antibacterial bioactive compounds in Withania roots and Basella leaves against Aeromonas infection in Nile tilapia. Withania ethanol extract (EW, ZoI 10.72 ± 1.028 mm) and Basella methanol extract (MB, ZoI 10.83 ± 1.058 mm) showed the highest antibacterial activity in disc diffusion assay. In in vivo experiment, Nile tilapia (25.61 ± 0.31 g) were fed basal, EW and MB (both at 0.7 g/kg feed) diets for 15 days either before being intraperitoneally injected with Aeromonas and basal diet feeding for 7 days (prophylactic) or after bacterial injection and basal diet feeding for 7 days (therapeutic). Fish fed EW and MB fortified diets showed significantly (p < 0.05) lower mortality, alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvate transaminase activities, HSP70, Caspase3, IFN-ɣ, IL-1 β, TNF-α level, while significantly (p < 0.05) higher weight gain, protein efficiency ratio, respiratory burst, lysozyme, phagocytotic activity, plasma total protein, total immunoglobulin content, RBC, WBC count, haemoglobin, haematocrit, mean corpuscular haemoglobin concentration, mean corpuscular volume, lymphocytes, neutrophils, monocytes percentages and superoxide dismutase, catalase, glutathione peroxidase activities compared to basal diet-fed fish. EW showed significantly (p < 0.05) better antibacterial efficacy compared to MB. Prophylactic treatment showed better results compared to therapeutic treatment. Bioassay-guided chromatography and GC–MS analysis revealed the presence of hexadecanoic acid, phytol, cis, cis, cis-7,10,13-hexadecatrienal in EW and α-tocopherol, 1-(+)-ascorbic acid 2,6-dihexadecanoate, 1-heptatriacotanol in MB, which was reported to have antibacterial, antioxidative and immunostimulatory potential. Present results indicate prospective use of EW as a prophylactic immunostimulant and effective therapeutic remedy against Aeromonas in Nile tilapia.

Metabolic Responses to Epinephrine by Periparturient Dairy Cows Fed Prepartum Diets Differing in Predicted Metabolizable Protein Supply

We determined the effects of prepartum dietary metabolizable protein (MP) supply on lipolytic and glucose response to epinephrine stimulus during the periparturient period. Twelve non-lactating cows in second or greater pregnancies were assigned to a low MP (LMP) diet formulated to provide ~900 g/day MP or a high MP (HMP) diet to provide ~1,100 g/day MP. Cows received prepartum diets from 28 days before expected parturition to day of parturition and then received a common lactation diet until 35 days postpartum. Blood was sampled weekly to day −7, daily to day 7, and weekly through day 35 relative to parturition for analysis of glucose, non-esterified fatty acids (NEFAs), 3-hydroxybutyrate (BHB), urea N, and total protein concentrations. Epinephrine challenges were conducted on day −10 before expected parturition and days 7 and 14 postpartum. Epinephrine [1.4 μg/kg body weight (BW)] was administered via jugular vein and blood was collected from the opposite jugular vein from −45 min to +120 min relative to epinephrine administration. Plasma was analyzed for glucose and NEFA concentrations. Prepartum dry matter intake (DMI) (14.7 and 12.6 ± 1.5 kg/day for LMP and HMP, respectively), postpartum DMI (21.4 and 19.4 ± 1.3 kg/day for LMP and HMP, respectively), and 4% fat-corrected milk (FCM) (37.0 and 36.8 ± 1.5 kg/day for LMP and HMP, respectively) did not differ significantly between diets. Cows fed HMP had higher plasma concentrations of urea N prepartum than cows fed LMP (14.0 vs. 7.9 ± 0.8 mg/dl). Cows fed HMP tended to have greater prepartum concentrations of total protein in plasma than those fed LMP (7.5 vs. 7.0 ± 0.2 g/dl). The area under the curve (AUC) for NEFA response to epinephrine did not differ between diets, but differed by day relative to parturition [8,284, 29,018, and 18,219 ± 2,302 min × (μeq/l) for days −10, 7, and 14, respectively]. Maximal response of NEFA concentration to epinephrine was greater for HMP than for LMP (744 vs. 438 ± 72 μeq/l). The glucose AUC did not differ between diets or among days. Lipolytic response, but not glucose response, to epinephrine was enhanced during the early postpartum period relative to the late dry period. However, those responses were not affected by prepartum MP supply.

Antidiabetic and Antihyperlipidemic Effects of Methanolic Extract of Leaves of Spondias mombin in Streptozotocin-Induced Diabetic Rats.

Objective: Spondias mombin is a plant that reported to have anticonvulsant, antimicrobial, antioxidant, antiulcer, antiasthmatic, and wound healing activities. Diabetes dyslipidemic effect of Spondias mombin leaves is not clear. Hence, current study planned to evaluate the antidiabetic and antihyperlipidemic effects of methanolic extract of leaves of Spondias mombin (MESM) in streptozotocin (STZ) induced diabetic rats. Methods: Phytochemicals were determined by standard method and antioxidant activity was determined by DPPH free radical scavenging and FRAP assay. Diabetes was induced by injecting a single dose of STZ (55 mg/kg) into female sprague dawley rats. After 3 days of induction of diabetes, the diabetic animals were treated for 28 days with MESM (125, 250, and 500 mg/kg) and glibenclamide (20 mg/kg) orally. The body weight of rats and blood glucose levels were monitored at regular intervals during the experiment. At the end of study, blood sample was collected from all the animals and subjected to biochemical, lipid profile, and they were sacrificed and their organs such as pancreas, liver and kidney were used for histopathological analysis. Results: Quantitative analysis of MESM showed the presence of anthraquinone, tannins, saponins, steroid, phenols, flavonoids, alkaloids, and reducing sugars. Reduction in body weight and elevated blood glucose were observed in diabetic rats. Treatment with MESM in a concentration of 125, 250, and 500 mg/kg significantly reversed the elevated levels of blood glucose, reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, urea, creatinine, total serum cholesterol (TC), serum triglyceride (TG), low-density lipoprotein (LDL), Very low-density lipoprotein (VLDL), and increased plasma insulin, total protein, albumin, globulin, A/G ratio, and high-density lipoprotein (HDL). Conclusion: MESM exhibited a significant antidiabetic and antihyperlipidemic activities against STZ-induced diabetes in rats.

Effectiveness of Short-Term Heat Acclimation on Intermittent Sprint Performance in the Heat with Moderately Trained Males

Purpose: Effectiveness of short-term heat acclimation (STHA), over 5-days (permissive dehydration), on intermittent heat stress test (HST) with males. Methods: Ten, moderately-trained, males (mean [SD]; age 25.6 [8.9] y; stature 180.7 [5.6] cm; body mass 83.2 [10.8] kg; and 45.3 [6.5] mL.kg-1.min-1) participated. The HST was 9 x 5min (45-min) of intermittent exercise based on professional soccer players. One week apart, HST1 vs HST (11.0°C; 50%RH), as a reliability trial and HST3 in 31.0°C; 50%RH were completed. Then 90 min dehydration, STHA (no fluid intake), for 5 consecutive days (39.5oC; 60%RH), using controlled-hyperthermia (~rectal temperature [Tre] 38.5oC). The HST4 within one week after STHA. Blood plasma constituents: percent plasma volume (%PV), aldosterone, total protein, albumin, electrolytes, cortisol and HSP70. Data analysis reported as mean differences with 95% confidence intervals (95%CI) and Cohen’s d effect size. Results: Post STHA, there was a decrease of -0.20 Tre at 45-min in the HST (95%CI -0.40 to -0.05°C; P=0.03; d =-0.56); mean skin temperature (-0.80; -1.30 to -0.30°C; P=0.007; d =-1.46) and mean body temperature (-0.30; -0.50 to -0.10°C, P=0.01; d =-0.75). Cardiac frequency reduced (-3: -5 to -1 b.min-1; P=0.01; d =-0.20) and %PV increased (7.3: 0.9 to 13.7%; P=0.03; d=0.59). Mean Peak Power (MPO) increased (Pd =0.63). Conclusions: Short-term heat acclimation (5-days) with dehydration, using controlled-hyperthermia technique, is effective for physiological adaptations during intermittent exercise in the heat, with moderately trained males.

Blue and Red Light Color Combinations Can Enhance Certain Aspects of Digestive and Anabolic Performance in Juvenile Steelhead Trout Oncorhynchus mykiss

The light spectrum varies with the altitude of the sun and shows different light colors in clear water. In this study, we aimed to investigate the response of juvenile steelhead trout Oncorhynchus mykiss (34.67 ± 2.69 g initial weight) under different light color conditions. The effects of different blue and red light combinations on plasma biochemical parameters, digestive enzyme activity, and RNA/DNA ratio were assessed in trout over 16 weeks. Six treatments were randomly assigned to 24 tanks with four replicates per treatment: a constant light intensity of 150 lx: 12 h white light then 12 h dark (12W); 12 h blue light then 12 h dark (12B); 12 h red light then 12 h dark (12R); 1.5 h blue light, 9 h red light, 1.5 h blue light, then 12 h dark (3B9R); 3 h blue light, 6 h red light, 3 h blue light, then 12 h dark (6B6R); and 12 h of both blue and red light then 12 h dark (T12BR). Fish exposed to the 3B9R light environment showed significantly increased plasma levels of total protein (TP), enhanced activities of midgut lipase, trypsin, and gastric lipase; and increased RNA content in the liver and muscle tissue to promote protein synthesis efficiency, thereby improving digestive and anabolic performance compared to fish in the other treatments. This indicates that steelhead trout have adapted well to such variable light conditions during long-term evolution. In contrast, trout exposed to the 6B6R light environment showed significant reductions in plasma glucose, TP, and triglyceride levels, decreased activity of gastrointestinal digestive enzymes, and reduced protein synthesis capacity in the muscle and liver, resulting in weakened digestive and anabolic performance. Furthermore, despite the high RNA content and RNA/DNA ratio in fish exposed to a 12R light environment, relatively high plasma cholesterol and triglycerides levels were observed, which might indicate oxidative stress. Therefore, this light is not considered suitable for long-term cultivation. In conclusion, the 3B9R treatment was the optimal light condition tested and can be used to improve the digestive and anabolic performance of steelhead trout.

Feeding meat goats mangosteen (Garcinia mangostana L.) peel rich in condensed tannins, flavonoids, and cinnamic acid improves growth performance and plasma antioxidant activity under tropical conditions

ABSTRACT The aim of this study was to evaluate the growth performance and plasma antioxidant activity of meat goats fed diets containing mangosteen peel powder (MPP; 41.25 g/kg dry matter). Twelve goats (body weight, 23.1 ± 1.49 kg) were fed a total mixed ration for 0–38 days under a severe temperature–humidity index (temperature, 80.96–103.64°F; humidity, 63%). Body weight and samples of feed, rumen fluid, blood, faeces, and urine were analysed. The MPP diet included condensed tannins, flavonoids, and cinnamic acid. Compared to the control group (CTL; no MPP), dietary MPP increased the nutrient digestibility of organic matter and body weight percentage of goats. Total volatile fatty acids, with a higher proportion of acetic acid, were higher in the MPP group; however, pH and NH3-N remained unchanged for both groups. Methane production in the rumen was reduced in the MPP group. Dietary MPP partly improved biochemical indicators such as plasma total protein (PTP) and albumin (ALB), and modulated antioxidant activity by increasing the glutathione peroxidase concentration and 2,2-diphenyl-1-picrylhydrazyl scavenging activity in plasma. These findings indicate that feeding meat goats the proposed MPP diet represents an environmentally friendly strategy for improving growth performance and plasma antioxidant activity under a severe temperature–humidity index.

PENGARUH SUPLEMENTASI TEPUNG KROKOT (Portulaca oleraceae l) DENGAN TARAF YANG BERBEDA TERHADAP KADAR TOTAL PROTEIN PLASMA, ALBUMIN DAN GLOBULIN KAMBING JAWARANDU (Capra aegagrus hircus)

This study aimed to determine the best dose of purslane flour on total plasma protein, albumin, and globulin in Jawarandu Goats. This research was conducted in February-March 2021 at the Rambon Asri Farmer Group, Rejo Asri Village, Seputih Raman District, Central Lampung Regency, Lampung Province. Examination of total plasma protein, albumin, and globulin was carried out at the Veterinary Center of Lampung Province. The experimental design used was a completely randomized design (CRD) with 4 treatments and 5 replications. The treatments were basal ration (P0), basal ration with 5% purslane flour supplementation (P1), basal ration with 10% purslane flour supplementation (P2), and basal ration with 15% purslane flour supplementation (P3). The data obtained were analyzed using analysis of variance with a significance level of 5%. The results showed that purslane flour supplementation had no significant effect (P&gt;0.05) on total plasma protein, albumin, and blood globulin of Jawarandu Goats but remained in the normal range.&#x0D; &#x0D; Keywords : Albumi, Globulin, Jawarandu goat, Purslane flour, Total plasma protein

Total Protein, Secretory Immunoglobulin A and Lactoferrin Concentrations in the Breastmilk of Lactating Women and their Correlation with Nutritional Intake

Background and objectives: Mothers’ breastmilk is the best source of nutrition for infants; hence, its nutritional adequacy is essential. The purpose of this study is to evaluate the total protein, secretory immunoglobulin A (sIgA) and lactoferrin (LF) concentrations of breastmilk from lactating mothers in Jakarta and analyse their correlation with average daily calory, macronutrient and vitamin C and E intakes. Methods and study design: A cross-sectional study was conducted in two primary health centres in Jakarta, Indonesia. The breastmilk’s total protein concentration was measured with the Warburg–Christian method, while sIgA and LF concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Macronutrient and vitamin C and E intake was assessed through 24-hour food recall and a semi-quantitative food frequency questionnaire (FFQ), respectively. Results: Thirty-six lactating women were recruited. The mean plasma protein was 8.17 g/dl, breastmilk protein was 3.36 g/dL, sIgA was 4.37 g/L and LF was 14.36 g/L. The total energy, carbohydrate, protein, fat and vitamin C and E daily intakes were 1828.61 kcal/day, 234.76, 71.15, 72.31 gram/day, 109.7 mg/day and 5.60 mg/day, respectively. Neither plasma and breastmilk total protein nor sIgA and LF concentrations were correlated with total energy, macronutrients or vitamin C and E daily intake. Conclusions: The plasma total protein, breastmilk total protein, sIgA and LF concentrations of lactating women in Jakarta were within the normal range. These biochemical profiles were shown to be not significantly correlated with daily calory, macronutrient or vitamin C or E intake. Keywords: breastmilk protein; secretory immunoglobulin A; lactoferrin; macronutrients; vitamins

Effect of Using Avocado and Chia Oil and their Mixture in Meat Broiler Diets on Some Physiological and Microbial Characteristics of Blood Plasma

This study was conducted in the field of the Poultry Research Station of the Department of Animal Production / Department of Agricultural Research / Ministry of Agriculture for the period 4/4/2021 to 16/5/2021, in which 300 one-day-old Ross308 chicks that fed on diets used avocado oil and Chia with percentages 0, 0.2, 0.4, 0.6% respectively, and their mixture consisting of 0.0, 0.1, 0.2, 0.3 each of avocado and Chia oil (50% avocado + 50% Chia oil). The experiment included 4 treatments with 3 replicates for each treatment (10 birds/replicates), in order to study the effect of using avocado and chia oil and their mixture in meat broiler diets on some physiological and microbial characteristics of blood plasma. The results indicate a significant increase (P &lt; 0.01) in the concentration of total protein and globulin for the treatment birds in which the mixture of avocado and Chia oil was used. While there were no significant differences in the concentration of albumin between the types of the used oils. It was noted through the results that the percentage of usage 0.6% had achieved a high significant increase in the concentration of total protein, albumin and globulin in the blood plasma of the birds of these treatments compared with the rest of the percentages of use (0, 0.2 and 0.4). As for the interaction, the results showed a high significant increase in the protein concentration (P&lt;0.01) in the treatment (M0.6) in both total protein and albumin concentration compared with the rest of the interactions, And the concentration of globulin was significantly increased (P&lt;0.01) in the birds of the treatments (C0.6, M0.2, M0.4, M0.6) compared with the rest of the interactions. The results showed a high significant decrease (P&lt;0.01) in the concentration of cholesterol in the birds of the treatments in which the mixture of oil and Chia oil were used, while a high significant increase (P &lt; 0.01) in the concentration of HDL was observed in the birds that used the mixture of avocado and Chia oil in their diets compared with the other types of oils, While a high significant decrease (P&lt;0.01) in LDL concentration was observed in birds of the treatment with the oil mixture, while no significant differences were observed between types of oils in the concentration of both triglycerides and VLDL. The level of the percentage of usage had a high significant decrease (P &lt; 0.01) in the concentration of cholesterol, triglycerides, LDL and VLDL at all levels of usage 0.2, 0.4 and 0.6% compared to the control treatment, while a high significant (P &lt; 0.01) increase in the concentration of HDL was observed at the rates of use of 0.4 and 0.6% compared with the control treatment which is void of usage. Whereas, the best high significant improvement (P&lt;0.01) in the lipid profile of the lipid plasma was achieved by a mixture treatment with a percentage of use of 0.6% compared with the rest of the interactions. It was noted that there was a high significant increase (P &lt; 0.01) in the number of Lactobacillus bacteria and a high significant decrease (P &lt; 0.01) in the number of E. coli bacteria in the avocado and Chia oil mixture treatments compared with the rest of the used oils. As for the study of the effect of the percentage of use, it increased significantly. (P &lt; 0.01) Lactobacillus bacteria numbers of all usage percentages (0.2, 0.4 and 0.6) compared with the control treatment, in addition to a high significant decrease (P &lt; 0.01) in the numbers of E. coli bacteria. As for the interaction between the type of oil and the percentage of usage, a high significant increase (P &lt; 0.01) was observed in the numbers of Lactobacillus bacteria in the treatment (M0.2 and M0.6) compared with the rest of the interactions, in addition to a high significant decrease (P &lt; 0.01) In the numbers of E.coli bacteria in treatment (M0.6) as the best interaction compared to the rest of the interactions. We conclude from this study that the use of mixed oil at a percentage of 0.4-0.6% in meat broiler diets will improve the physiological characteristics of the blood plasma, in addition to a decrease in the number of harmful bacteria (E. coli) and an increase in the number of beneficial bacteria, which has an important role in improving the physiological condition of birds, which is reflected positively on productive performance and obtaining high growth without the use of harmful synthetic materials.

Comparative Study of Physiological Changes in Turbot Scophthalmus maximus in Different Living Conditions

The aim of this study was to compare the organismal responses of farmed and wild-caught turbot, Scophthalmus maximus, based on analyses of biochemical plasma parameters, leukocyte profile, and histological tissue profile of gills, kidney, liver, intestine, and spleen, as well as gene expression of stress proteins in kidney and liver tissue. The results revealed significant differences in plasma triglycerides (TRIG), total protein (TP), albumin (ALB), globulin (GLOB), bilirubin (TBIL), creatinine (CRE) levels, creatine kinase (CK), and superoxide dismutase (SOD) activities that were higher, and A/G ratio, calcium (Ca) and phosphorus (P) concentrations, alkaline phosphatase (ALP), and glutathione peroxidase (GPx) activity, which were lower in farmed population. The neutrophil-leukocyte (N:L) ratio and gene expression of HSP70, HSP90, and WAP65-2 were increased in the wild-caught turbot. The wild-caught turbot were infested with the gill digenean parasite Dactylogyrus sp. and tapeworm Bothriocephalus scorpii. The obtained results provide valuable data for the assessment of the physiological responses of turbot for future comparative studies of the effects of various endogenous and exogenous factors on homeostasis of this species.

Influence of tomato processing by-product extract as dietary supplementation on growth performance, carcass characteristics and antioxidant status of growing rabbits under high ambient temperature

This study evaluated the effects of dietary supplementation of tomato processing by-product extract (TPBE) on growth performance, carcass characteristics and antioxidant status of growing rabbits under high ambient temperature. A total of eighty weaned New Zealand White (NZW) male rabbits (6-weeks-old; initial body weight, 730.28 ± 36.05 g) were randomly assigned to 4 groups. The first group was the control without supplementation; while the other groups were fed diets supplemented with 100, 200 and 250 mg TPBE/kg. The results showed that TPBE contained 211.85 mg/100g as total phenols and total flavones of 303.36 mg/100g. Rabbits fed a 250 mg TPBE-supplemented diet showed the heaviest body weight, the lowest feed intake and the best feed conversion ratio. TPBE diets reduced mortality percentage. Dietary supplementation of 250 mg TPBE had the highest dressing percentage. Plasma total protein, globulin, catalase and glutathione peroxidase of rabbits fed diets supplemented with 200 and 250 mg TPBE were high. Plasma total cholesterol, triglycerides, plasma hydrogen peroxide and malondialdehyde concentrations were decreased with dietary levels of TPBE. Rabbits fed 250 mg TPBE had higher T-AOC than the other groups. TPBE supplemented diets improved net revenue and economic efficiency. Conclusively, TPBE is containing appreciable content of polyphenols and flavonoids and the dietary supplementation of TPBE (250 mg/kg diet) had a positive impact on growth performance, reducing mortality and enhancing the antioxidant status of rabbits reared under high ambient temperature.

Clinical manifestations, laboratory findings, treatment and outcome of acute organophosphate or carbamate intoxication in 39 cats.

Organophosphates and carbamates are important sources of intoxication for humans and animals. However, large-scale studies of these intoxications in cats are unavailable. The medical records of 39 cats presented to a veterinary teaching hospital with acute organophosphate or carbamate intoxication were reviewed retrospectively. Mortality in intoxicated cats was 15%. Low respiratory rate and low rectal temperature at presentation were associated with death. Other common clinical signs included weakness, ataxia, apathy, recumbency, anorexia and bradycardia, but these were unassociated with the outcome. The common biochemical abnormalities included decreased serum butyryl-choline esterase activity, acidaemia, hypercarbaemia and total hypocalcaemia, and increased creatine kinase activity and total plasma protein concentration. There were no significant differences in haematological, biochemical and blood gas analytes between survivors and non-survivors. Common medications and treatments included 2-pyridine aldoxime methyl-chloride-pralidoxime (2-PAM) (74%), metoclopramide (64%), antibiotics (64%), diphenhydramine (59%) and atropine sulphate (54%). There were no significant drug and treatment differences between survivors and non-survivors. The secondary complications of the intoxication included pneumonia (10%), acute kidney injury (10%) and pancreatitis (8%). Acute cholinergic crisis due to organophosphate or carbamate intoxication has a fair prognosis in cats. Low respiratory rate and low rectal temperature at presentation were associated with death. The most commonly used specific medications in this study included 2-PAM, diphenhydramine and atropine sulphate.