Plasma Antioxidant Power Research Articles

Lipid Peroxidation as a Possible Factor Affecting Bone Resorption in Obese Subjects-Preliminary Research.

Oxidative stress, which promotes bone catabolism, also affects the quality of bone tissue. We aimed to assess the impact of metabolic disorders and oxidant-antioxidant imbalance associated with primary obesity on bone resorption and formation processes. Anthropometric parameters, metabolic variables, oxidative stress indicators (malondialdehyde, vitamins A and E, uric acid, superoxide dismutase, catalase, glutathione peroxidase, type 1 paraoxonase, iron-reducing plasma antioxidant power) and markers of bone turnover (type I procollagen N-terminal propeptide and the type I collagen C-terminal cross-linked telopeptide; P1NP and CTX) were assessed in 108 Polish participants. Under the influence of oxidative stress, both enzymatic and non-enzymatic defense mechanisms were stimulated in obese subjects, especially in women, who had increased lipid peroxidation and activity of catalase (particularly in first-degree obesity) and decreased vitamin E concentration. The process of lipid peroxidation, as well as the weakening of the bone formation, was strongly manifested in women at a BMI range of 35.0-39.9 kg/m2 but not at BMI > 40.0 kg/m2, but it had a comprehensive negative impact on bone turnover in obese men. Obesity and its degree of advancement significantly affected the decrease in the concentration of the marker of bone formation-P1NP-only in the plasma of women. Excessive body weight had no effect on the value of the bone resorption marker in plasma, regardless of gender. Our results confirm the existence of the "obesity paradox" in the aspect of bone tissue metabolism and suggest that a specific body weight threshold changed the molecular response of the tissue.

Antioxidants in single methylene‐blue‐treated plasma units cannot be used to predict pathogen inactivation treatment success

Measurement of antioxidant power (AOP) can be useful to validate the execution of the pathogen inactivation (PI) treatment of plasma units. The aim of this study was to evaluate the Theraflex technology for plasma units routinely used in Belgium. AOP was tested on plasma units treated by Theraflex with various non-complete treatment scenarios. AOP was quantified electrochemically using disposable devices and was expressed as equivalent ascorbic acid concentration. During a complete PI treatment, AOP rose from 195 ± 32 to 230 ± 42 μmol/L eq. ascorbic acid after addition of methylene blue (MB), and decreased to 192 ± 30 μmol/L eq. ascorbic acid after illumination and finally to 177 ± 27 μmol/L eq. ascorbic acid after final filtration. Without MB, the final filtration had no effect on the plasma AOP (197 ± 22 μmol/L eq. ascorbic acid before filtration and 194 ± 22 μmol/L eq. ascorbic acid after filtration). With no MB and no illumination, there was no significant difference between the plasma AOP at the beginning (188 ± 23 μmol/L eq. ascorbic acid) and at the end of the process (179 ± 21 μmol/L eq. ascorbic acid). AOP measurement may not indicate the effectiveness of the PI treatment.

Changes in Plasma Fatty Acids, Free Amino Acids, Antioxidant Defense, and Physiological Stress by Oleuropein Supplementation in Pigs Prior to Slaughter.

Olive tree leaves are characterized for having not only a potent antioxidant power but also effects on glucose and lipid metabolism. The impact of the individual oleuropein (OLE), vitamin E + Se (VE), or a combined supplementation of oleuropein, vitamin E, and selenium (VEOLE) was evaluated on pig plasma metabolites under fasting prior to slaughter. VEOLE and OLE had lesser n-3 plasma polyunsaturated fatty acids and greater monounsaturated free fatty acids compared to control. The n-3-fatty acid mobilization was directly correlated with greater cystine and inversely with oxidized glutathione/reduced glutathione (GSSH/GSH) levels. This faster use of n-3 fatty acids might act as an indicator of glutathione synthesis mediated by an increase of cystine in plasma. Different correlations and linear adjustments were observed between plasma antioxidant power and free cystine, free glycine, free glutamine, monounsaturated free fatty acids, and total n-3. The best response to stress was found in VEOLE. Cortisol reached the greatest positive correlation with plasma total n-3 fatty acids, which suggests a faster uptake of n-3 for biological functions such as stress control or energy supply in the brain. From a practical point of view, an enhanced oxidative status as well as control of physiological stress prior to slaughter by the combined antioxidants supplementation might have positive effects on pork quality.

Outpatient evaluation of oxidative stress in subjects undergoing systemic oxygen-ozone therapy

Oxygen-ozone therapy improves the quality of life in patients affected by various diseases. The purpose of this study was to verify and eventually quantify the production of free radicals generated by oxidative stress induced by the ozonated auto-hemoinfusion in subjects subjected to this practice and eventually evaluate and naturally quantify the plasma antioxidant power of the subjects under examination. All subjects showed a significant improvement in the oxidative balance already at the first check, which was maintained (albeit with a minimal non-significant loss) at the end of the study. The results of the study have made it possible to verify that systemic ozonated auto-hemoinfusion not only does not involve further oxidative stress but even exerts an important antioxidant effect, through the scavenger effect of free radicals.

Protective properties of Myrtus communis extract against oxidative effects of extremely low-frequency magnetic fields on rat plasma and hemoglobin

Purpose: This study investigates the protective properties of Myrtus communis extract against the oxidative effects of extremely low-frequency magnetic fields (ELFMF). Also, this study is aimed to analyze the conformational changes of hemoglobin, oxidative damages to plasma proteins and antioxidant power of plasma following exposure to ELFMF.Materials and methods: Adult male rats were divided into 3 groups: (1) control, (2) ELFMF exposure, and (3) ELFMF exposure after M. communis extract administration. The magnetic field (0.7 mT, 50 Hz) was produced by a Helmholtz coil for one month, 2 hours a day. The M. communis extract was injected intraperitoneally at a dose of 0.5 mg/kg before exposure to ELFMF. The oxidative effects of ELFMF were studied by evaluating the hemoglobin, methemoglobin (metHb) and hemichrome levels, absorption spectrum of hemoglobin (200–700 nm), oxidative damage to plasma proteins by measuring protein carbonyl (PCO) levels and plasma antioxidant power according to the ferric reducing ability of plasma (FRAP). The mean and standard errors of the mean were determined for each group. One-way ANOVA analysis was used to compare the means of groups. The significance level was considered to be p < .05. Moreover, artificial neural network (ANN) analysis was used to identify the predictive parameters for estimating the oxyhemoglobin (oxyHb) concentration.Results: Exposure to ELFMF decreased the FRAP which was in concomitant with a significant increase in plasma PCO, metHb and hemichrome concentrations (p < .001). Oxidative modifications of Hb were shown by reduction in optical density at 340 nm (globin-heme interaction) and 420 nm (heme-heme interaction). Administration of M. communis extract increased FRAP values and decreased plasma POC, metHb, and hemichrome concentrations. Also, a significant increase in Hb absorbance at 340, 420, 542, and 577 nm showed the protective properties of M. communis extract against ELFMF-induced oxidative stress in erythrocytes. ANN analysis showed that optical absorption of hemoglobin at 520, 577, 542, and 630 nm and concentration of metHb and hemichrome were the most important parameters in predicting the oxyHb concentration.Conclusions: Myrtus communis extract enhances the ability of erythrocytes and plasma to deal with oxidative conditions during exposure to ELFMF. Also, ANN analysis can predict the most important parameters in relation to Hb structure during oxidative stress.

In vivo evaluation of effect of anthocyanin-rich wheat on rat liver microsomal drug-metabolizing cytochromes P450 and on biochemical and antioxidant parameters in rats

Wheat is one of the most important agricultural crops worldwide. Recently, anthocyanin-rich wheat genotypes were introduced to the market as a food with added value. In the experiment described here, two different genotypes with blue grain were studied (UC66049 and Skorpion). The aim of the investigation was to determine whether a 72-day-long intake of anthocyanin-rich wheat can influence the drug-metabolizing microsomal cytochromes P450 (using standard drug substrates of rat/human liver microsomal cytochromes P450) and antioxidant status, which indicated the ability of the rats' liver to metabolize drugs or xenobiotics. Consumption of an anthocyanin-rich diet moderately increased the activity and mRNA expression of the studied CYPs by 20–55% relative to the control (with the exception of CYP2B1/2). The health status of rats was good; the weight gain was lower in the group with anthocyanin-rich UC66049 grain compared to the other two groups (control and Skorpion-fed one). The antioxidant power of plasma was mildly but significantly higher in the test groups, other antioxidant parameters remained unaffected, except for an increased level of total SH groups. Based on our observations, there will most likely be no significant influence of the consumption of anthocyanin-rich wheat on the metabolism of xenobiotics and drugs.

Determination of Plasma Antioxidant Power in Capillary Blood through the Innovative system PAT (Plasma Antioxidant Test)

Background: Plasma antioxidants (AOs) represent the main barrier of defense against damaging aggression due to reactive species, by reactive oxygen species (ROS). Moreover, changes in AOs plasma content after supplementation may provide information on the absorption and bioavailability of nutritional compounds and efficacy of AOs therapy. Aim: The aim of this study was to make a comparison between the known BAP test (Biological Antioxidant Potential), used for the evaluation of the antioxidant capacity, and the innovative PAT (Plasma Antioxidant Test), and the evaluation of the precision, linearity and accuracy of the new patented PAT on plasma samples taken from healthy volunteers. Materials and Methods: Twenty-two apparently healthy volunteers were involved in the study for the comparison of the two methods. Results: Analysis performed on 22 plasma samples demonstrated that, following the application of the algorithm, BAP and PAT were interchangeable. PAT resulted extremely precise, linear (R=0.9991), accurate and reproducible (CV 4.17%). PAT values from plasma samples had a normal distribution, evidencing a control range for healthy subjects. Conclusion: PAT can be considered an innovative and predictable method for the measure of the antioxidant power of plasma.

Alteration of hepatocellular antioxidant gene expression pattern and biomarkers of oxidative damage in diazinon-induced acute toxicity in Wistar rat: A time-course mechanistic study.

In the present survey, the plasma level of diazinon after acute exposure was measured by HPLC method at a time-course manner. In addition, the impact of diazinon on the expression of the key genes responsible for hepatocellular antioxidative defense, including PON1, GPx and CAT were investigated. The increase in oxidative damages in treated rats was determined by measuring LPO, protein carbonyl content and total antioxidant power in plasma. After administration of 85 mg/kg diazinon in ten groups of male Wistar rats at different time points between 0-24 hours, the activity of AChE enzyme was inhibited to about 77.94 %. Significant increases in carbonyl groups and LPO after 0.75 and 1 hours were also observed while the plasma antioxidant power was significantly decreased. Despite the dramatic reduction of GPX and PON1 gene expression, CAT gene was significantly upregulated in mRNA level by 1.1 fold after 4 hours and 1.5-fold after 24 hours due to diazinon exposure, compared to control group. Furthermore, no significant changes in diazinon plasma levels were found after 4 hours in the treated rats. The limits of detection and quantification were 137.42 and 416.52 ng/mL, respectively. The average percentage recoveries from plasma were between 90.62 % and 95.72 %. In conclusion, acute exposure to diazinon increased oxidative stress markers in a time-dependent manner and the changes were consistent with effects on hepatic antioxidant gene expression pattern. The effect of diazinon even as a non-lethal dose was induced on the gene expression of antioxidant enzymes. The change in antioxidant defense system occurs prior to diazinon plasma peak time. These results provide biochemical and molecular evidence supporting potential acute toxicity of diazinon and is beneficial in the evaluation of acute toxicity of other organophosphorus pesticides as well.

Effects of dietary lipid-coated zinc on the antioxidant defense system in the small intestine and liver of piglets

The purpose of the study was to investigate the effects of lipid-coated ZnO (LCZ) and the level of LCZ compared with ordinary zinc oxide (ZnO) on antioxidant defense system in the intestine and liver of piglets. A total of forty piglets (n=8) were fed a diet supplemented with 100 ppm Zn with ZnO (ZnO-1), 2,500 ppm Zn with ZnO (ZnO-2), 100 ppm Zn as LCZ (LCZ-1), 200 ppm Zn as LCZ (LCZ-2), or 400 ppm Zn as LCZ (LCZ-3) for 14-d, respectively. The LCZ-3 group resulted in higher (P<0.05) mRNA expressions and activities of CuZn-superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and glutathione S-transferase (GST) in jejunal mucosa compared with the ZnO-1 and LCZ-1 groups, while no difference was observed in the mRNA level of antioxidant genes between the ZnO-1 and ZnO-2 groups. Within the LCZ groups, the LCZ level linearly and quadratically (P<0.01) increased antioxidant enzymes in the jejunum. The maximum response of jejunal antioxidant enzymes to Zn supplementation was achieved by 400 ppm of LCZ. Hepatic mRNA expression of antioxidant enzymes was unaffected by Zn source and level, while hepatic SOD and GST activities were greater (P<0.05) in the LCZ-3 group than in the ZnO-1 group. No difference was observed in lipid peroxidation of the jejunum and liver and the total antioxidant power of plasma among groups. In conclusion, a supplementation with 400 ppm of LCZ resulted in a maximum increase in antioxidant enzymes, indicating that LCZ may affect antioxidant defense system more profoundly than ZnO.

Effects of feeding different levels of dried orange pulp and recycled poultry bedding on the performance of fattening lambs.

The effects of dietary feeding different levels of recycled poultry bedding (RPB) and dried orange pulp (DOP) on the performance of fattening Shal lambs (initial BW of 28 ± 2.2 kg) were assessed using a factorial experiment (2 × 3) with a completely randomized design. Six diets, containing different levels of RPB (0 or 160 g/kg of DM) and DOP (0, 110, or 220 g/kg of DM), were randomly assigned to 6 groups of 6 lambs each for a 74-d period (14 d for adaptation and 60 d for data collection). Diets were offered as total mixed ration to ensure 10% of orts. Feed intake, growth, diet digestibility, microbial nitrogen (N) synthesis (MNS), N retention, rumen fermentation parameters, and blood metabolites were determined. Data were analyzed using the PROC MIXED of SAS. Increasing RPB and DOP levels in the diet of the lambs improved ( < 0.05) DMI, ADG, and G:F. Diet digestibility was not affected by dietary feeding of RPB ( > 0.05), but increased as DOP level increased in the diet ( < 0.05). Feeding RPB and DOP had no effects ( > 0.05) on the ruminal pH. The ruminal total VFA concentration, molar proportion of acetic acid, and acetate-to-propionate ratio increased ( < 0.05) with the increasing dietary levels of RPB and DOP, while ammonia N concentration, propionic acid proportion, total protozoa count, and subfamily number decreased ( < 0.05). Including RPB and DOP in diet enhanced ( < 0.05) MNS and N retention. Feeding RPB-containing diets caused ( < 0.05) an increase of plasma glucose concentration and decreases ( < 0.05) of cholesterol and urea N. As the dietary level of DOP increased, the plasma concentrations of glucose, triglycerides, and urea N reduced ( < 0.05). The plasma antioxidant power was not affected ( > 0.05) by feeding RPB, but increased ( < 0.05) with increasing DOP level in diet. The animal performance reached its greatest value with simultaneous feeding of 160 g of RPB and 220 g of DOP per kg of diet DM. It has been concluded that the partial dietary substitution of RPB (instead of soybean meal, wheat bran, and sugar beet pulp) or/and DOP (instead of barley grain and corn grain) in fattening Shal lambs had positive effects on feed intake, diet digestibility, ruminal microbial N, growth, and N retention. Synchronized feeding of the great levels of RPB (as an N source) and DOP (as an energy source) increased utilization efficiency of these byproducts in the fattening lambs.

Imbalance in the blood antioxidant system in growth hormone-deficient children before and after 1 year of recombinant growth hormone therapy.

The aim of our study was to examine the effects of 12-month therapy with recombinant growth hormone (rGH) on the blood antioxidant system in children with growth hormone deficiency (GHD). Total antioxidant capacity (TAC) of plasma was measured by FRAP (ferric reducing antioxidant power or ferric reducing ability of plasma); activities of superoxide dismutase (SOD) and catalase (CAT) in erythrocytes were assessed; non-protein thiols (NT) and ceruloplasmin (CP) levels were also measured. These parameters were determined before and after 12 month of rGH treatment. Eleven treatment-naive prepubertal children with growth hormone deficiency were included in the study. Another 11 prepubertal children comprised a control group. Before rGH treatment, TAC of plasma and NT level in the control group were significantly lower (726 ± 196 vs. 525 ± 166 µmol/L, P = 0.0182 and 0.92 ± 0.18 vs. 0.70 ± 0.22 µmol/ml, P = 0.0319, before and after the therapy, respectively). The only parameter that significantly (19.6 ± 4.7 vs. 14.5 ± 3.4 Units/g Hb, P = 0.0396) exceeded the same in the control group after rGH therapy was SOD activity. However, none of the measured parameters of antioxidant system in GHD children, except for TAC (525 ± 166 vs. 658 ± 115 µmol/L, P = 0.0205), exhibited significant improvement toward the end of the 12-month treatment period, although non-significant changes in CAT activity and CP level were also observed. This work has demonstrated that some parameters of the blood antioxidant system are out of balance and even impaired in GHD children. A 12-month treatment with rGH resulted in a partial improvement of the antioxidant system.

Estrogen plus progestin increase superoxide dismutase and total antioxidant capacity in postmenopausal women

Objective This cross-sectional study aimed to evaluate the behavior of blood antioxidant enzymes (superoxide dismutase (SOD), catalase and glutathione peroxidase), plasma total antioxidant capacity and oxidative damage (lipid oxidation and protein carbonyl levels) and their relationship with the serum levels of steroid hormones in premenopausal and postmenopausal women without and with estrogen alone (ET) or estrogen plus progestin therapy (EPT).Methods Blood was collected from four groups of subjects: premenopausal women (n = 24), postmenopausal women without hormone therapy (n = 31), postmenopausal women with ET (n = 12) and postmenopausal women with EPT (n = 16).Results The activities of the different SOD isoforms (CuZnSOD and MnSOD) and the plasma total antioxidant power were significantly higher in the postmenopausal women under EPT than in the postmenopausal women without hormone replacement therapy (HRT). Only CuZnSOD activity was increased in women receiving ET compared to the postmenopausal women without HRT. However, no differences were observed in the levels of lipid or protein oxidation or in the non-enzymatic plasma antioxidants (uric acid and albumin) among the groups. The duration of HRT and serum estrogen levels were positively correlated to the blood CuZnSOD activity and to plasma total antioxidant power, whereas the serum progesterone levels were positively correlated to CuZnSOD activity and negatively correlated to protein carbonyl groups. Interestingly, the total antioxidant power of plasma was positively correlated to CuZnSOD and glutathione peroxidase activities.Conclusion We conclude that EPT increases blood MnSOD and CuZnSOD activity in postmenopausal women, leading to an increased plasma total antioxidant capacity. This finding may be relevant to the prevention of oxidative stress-related disorders in postmenopausal women.

Impact of Swertia chirata extract on prevention of Cu2+ LDL oxidation and malondialdehyde formation

Diabetes is a multisystem disorder marked by elevated blood sugar level. It has been estimated that the global burden of type 2 diabetes mellitus (T2DM) for 2030 is projected to increase to 438 million ; a 65 % increase. During diabetes, persistent hyperglycemia causes increased production of free radicals especially reactive oxygen species (ROS) as by-products of normal cellular metabolism. The chronic hyperglycaemia, insulin resistance and abnormal lipoprotein profiles found in diabetes may contribute to a decrease of bioavability of vascular nitric oxide (NO), impairing endothelium- dependent vasodilatation documented in and in humans with diabetes. Oxidized LDL may contribute to the progression of atherosclerosis by enhancing endothelial injury.Extracts of chirata reduced LDL oxidation by about 40% which is very close to the efficacy of Atorvastatin (reduction by 51.72%).The management of diabetes nephropathy is extremely expensive and frustrating. Therefore, prevention is better. Sources of antioxidants, especially antioxidant vitamins are available and affordable in most environments. In present investigation aqueous and ethanolic Swertia chirata, increased total antioxidant power of normal plasma by 1.484 and 1.84 fold,and in diabetics it increased by 1.25 fold and 2.19 fold respectively. Extracts of chirata are also very effective in reducing MDA formation in plasma,also these are free of side effects and very cost effective.

Sulfur mustard induced oxidative stress and its alteration using asoxime (HI-6)

Sulfur mustard (SM) is a blister agent with cytotoxic mechanism of action. There is no suitable treatment based on administration of an antidote. In this study, Wistar rats were exposed to SM in doses of 0–40 mg/kg body weight and treated with the compound HI-6. The treatment provided no significant effect on ferric reducing antioxidant power of blood and plasma. However, HI-6 caused an increase in the level of thiobarbituric acid reactive substances. This stressogenic response was presumably the cause of the significant elevation of the blood level of both glutathione reductase and reduced glutathione. HI-6 appears to be suitable for enhancing prophylactically oxidative stress protection from small oxidative insult.

Effect of green tea on postprandial antioxidant capacity, serum lipids, C-reactive protein and glucose levels in patients with coronary artery disease

Cardiovascular risk factors have been identified in the postprandial state, particularly in patients with coronary artery disease (CAD). Tea consumption has been linked to cardiovascular risk reduction, but the beneficial effect of tea has not been investigated under postprandial conditions. The objective was to examine the effect of green tea on postprandial levels of plasma total antioxidant capacity (TAC), serum lipids, C-reactive protein (CRP) and glucose in patients with CAD. In a randomized controlled, parallel design with 2 arms, 43 patients with CAD were assigned to consume breakfast consisting of bread, butter and 330 ml water or tea (4.5 g green tea/330 ml, providing approximately 400 mg catechins). Blood samples were drawn immediately before and 1.5, 3 and 5 h after breakfast. TAC was measured in plasma with the ferric reducing antioxidant power of plasma and oxygen radical absorbance capacity assays. Total cholesterol, high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), triglycerides, glucose, CRP, uric acid and pancreatic lipase levels were measured in serum. Tested biomarkers did not differ between tea and water group at baseline, 1.5, 3 and 5 h (P > 0.05) postprandially. However, TAC increased 1.5 and 3 h after consumption of breakfast with tea (P < 0.005), but no change was observed after consumption of breakfast with water. Serum triglycerides levels significantly increased 3 h after breakfast with water (P = 0.031), but not after breakfast with tea. Serum uric acid decreased 1.5 h after breakfast with tea (P = 0.038). Pancreatic lipase, CRP, total cholesterol, HDL-C, LDL-C and glucose levels remained unchanged after breakfast with tea at any time point (P > 0.05). Tea consumption did not affect selected biomarkers at any postprandial time point in patients with CAD.

Phytosterol supplementation does not affect plasma antioxidant capacity in patients with metabolic syndrome

Several studies have observed decreased levels of lipophilic antioxidants after supplementation with phytosterols and stanols. The aim of this study was to examine the effect of phytosterol supplementation on plasma total antioxidant capacity in patients with metabolic syndrome. In a parallel arm, randomized placebo-controlled design, 108 patients with metabolic syndrome were assigned to consume yogurt beverage which provided 4 g of phytosterols per day or yogurt beverage without phytosterols. The duration of the study was 2 months and the patients in both groups followed their habitual westernized type diet. Blood samples were drawn at baseline and after 2 months, and the total antioxidant capacity of plasma was measured using the ferric reducing antioxidant power of plasma and oxygen radical absorbance capacity assays. After 2 months of intervention, plasma total antioxidant capacity did not differ between and within the intervention and the control groups. Phytosterol supplementation does not affect plasma antioxidant status.

Lipid-lowering effect of artichoke on liver phosphatidate phosphohydrolase and plasma lipids in hyperlipidemic rats

Artichoke ( Cynara scolymus L.) is full of natural antioxidants and has a lipid-lowering effect. The aim of this study was to investigate the effect of artichoke on the liver phosphatidate phosphohydrolase (PAP), plasma lipid levels, plasma malondialdehyde (MDA), and plasma antioxidant in hyperlipidemic rats. Male rats were fed by standard pellet diet (Group 1), standard diet supplemented with 10% artichoke (Group 2), lipogenic diet (containing sunflower oil, cholesterol and ethanol) plus 10% artichoke (Group 3) and only lipogenic diet (Group 4). On day 60 of the experiment, liver PAP activity, liver triglyceride (TG), plasma lipids, plasma MDA, and plasma antioxidant levels were measured. PAP activity, liver TG, the ratio of total cholesterol (TC) to high density lipoprotein (HDL) cholesterol, plasma TC and TG levels were significantly decreased due to artichoke treatment in Groups 2 and 3 compared to Groups 1 and 4, respectively. Significant reduction in plasma MDA and significant elevation in plasma antioxidant power observed in Groups 2 and 3 compared to Groups 1 and 4, respectively. The results clearly indicated that artichoke can be useful for the reduction of PAP activity and liver TG. Also, artichoke has beneficial effects in the controlling of hyperlipidemia, abnormalities in lipid profiles and oxidative stress in hyperlipidemic regimes.

Native immunity and oxidative traits of growing rabbits

The evaluation of animal welfare through innate immunity (Serum Bactericidal Activity – SBA, Hemolytic Complement Assay – HCA, lysozyme) and the antioxidant status of the body (Reactive Oxygen Substances – ROS and Antioxidant Power of plasma, AP) offers a reliable prognostic and diagnostic tool. The aim of the present study was to investigate trends and correlations between some traits of innate immunity and the oxidative status of fattening rabbits at different ages. Blood samples from 120 New Zealand White fattening rabbits at 45, 55, 65, and 75 d of age were collected and analyzed. The results showed that SBA did not have a normal distribution because of numerous 0 values. Data distribution was normal when only SBA > 0 values were considered. Lysozyme (mean value 27.19 μg/mL) and HCA (mean value 50.84 CH 50% ) had stable trends at different ages and showed a tendency that was comparable to that obtained in other animal species. On the contrary, SBA (mean value 42.15%) showed an unexpected positive correlation with lysozyme (P<0.001) and a negative correlation with HCA (P<0.001). Oxygen free-radicals are involved in the pathogenesis of several diseases and oxidative stress alters immune competence. In this experiment, ROS and AP showed mean values of 0.60 mmol H 2 O 2 and 421.67 μmol HClO, respectively. In this context positive correlation coefficients between oxidative status traits and immune traits (P<0.001) were found, although at a very low level; and surprisingly, only ROS and SBA did not show any significant correlation. In this study it emerged that, even in the absence of evident pathologies, the immune and oxidative traits of fattening rabbits could be affected by environmental stress (weaning, cage, neighbors)

Plasma protein oxidation and total antioxidant power in premenstrual syndrome

Objective To explore whether oxidative stress has any role in premenstrual syndrome (PMS). Methods Female volunteers suffering from PMS, in the age group of 20–24 years were compared to their asymptomatic normomennorhoeic counterparts in follicular phase and late luteal phase for ferric reducing antioxidant power of plasma (FRAP), plasma protein thiols (PPT) and protein carbonyls (PPC) levels. Results There was no significant change in FRAP and PPC levels in controls and PMS groups but PPT decreased significantly in luteal phase of PMS (P<0.05) when compared to follicular phase. Conclusions Estrogen and progesterone, might be responsible for a healthy antioxidant profile in PMS. However, a marked decrease in PPT in luteal phase of PMS group may be due to pro-oxidant nature of estrogen-active in this phase of PMS leading to consumption of the sacrificial antioxidant-protein thiol.

Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo ( Bubalus bubalis) sperm quality following cryopreservation

Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. Under the present study, the cryoprotective effect of taurine and trehalose on buffalo sperm quality parameters after freeze–thaw process was studied. Buffalo semen was cryopreserved in tris-based egg yolk extender along with cryoprotectants like taurine (50 mM) or trehalose (100 mM) and used for the assessment of sperm quality parameters like motility, viability, plasma membrane integrity, total antioxidant status and the extent of cryocapacitation. The results were compared to semen cryopreserved in tris-based egg yolk extender only as control. Post-thaw semen evaluation clearly indicated that the addition of taurine or trehalose significantly improved ( P < 0.05) the motility, viability and membrane integrity compared to control spermatozoa. The extent of sperm cells underwent cryocapacitation was significantly lowered ( P < 0.05) in presence of taurine or trehalose. Moreover, the percentage of in vitro capacitated cells in the treated samples was comparable to the control spermatozoa along with maintaining other sperm quality parameters. Finally, compared to the control and trehalose treated sample, addition of taurine to the freezing extender showed more positive effect on the total antioxidant power of seminal plasma and spermatozoa. It is concluded that the addition of taurine or trehalose to the freezing extender led to the reduction of cryodamage to the buffalo spermatozoa.