Abstract
The purpose of the study was to investigate the effects of lipid-coated ZnO (LCZ) and the level of LCZ compared with ordinary zinc oxide (ZnO) on antioxidant defense system in the intestine and liver of piglets. A total of forty piglets (n=8) were fed a diet supplemented with 100 ppm Zn with ZnO (ZnO-1), 2,500 ppm Zn with ZnO (ZnO-2), 100 ppm Zn as LCZ (LCZ-1), 200 ppm Zn as LCZ (LCZ-2), or 400 ppm Zn as LCZ (LCZ-3) for 14-d, respectively. The LCZ-3 group resulted in higher (P<0.05) mRNA expressions and activities of CuZn-superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and glutathione S-transferase (GST) in jejunal mucosa compared with the ZnO-1 and LCZ-1 groups, while no difference was observed in the mRNA level of antioxidant genes between the ZnO-1 and ZnO-2 groups. Within the LCZ groups, the LCZ level linearly and quadratically (P<0.01) increased antioxidant enzymes in the jejunum. The maximum response of jejunal antioxidant enzymes to Zn supplementation was achieved by 400 ppm of LCZ. Hepatic mRNA expression of antioxidant enzymes was unaffected by Zn source and level, while hepatic SOD and GST activities were greater (P<0.05) in the LCZ-3 group than in the ZnO-1 group. No difference was observed in lipid peroxidation of the jejunum and liver and the total antioxidant power of plasma among groups. In conclusion, a supplementation with 400 ppm of LCZ resulted in a maximum increase in antioxidant enzymes, indicating that LCZ may affect antioxidant defense system more profoundly than ZnO.
Highlights
Effects of dietary lipid-coated zinc on the antioxidant defense system in the small intestine and liver of piglets
The LCZ-3 group resulted in higher (P< 0.05) mR NA expressions and activities of CuZn-superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAn, and glutathione S-transferase (CST) in jejunal mucosa compared with the ZnO-l and LeZ-l groups, while no difference was observed in the mRNA level of antioxidant genes between the ZnO-1 and ZnO-2 groups
MRNA expression and activity of antioxidant genes in the intestine and liver The effects of dietary supplementation with ZnO (ZnO-1 and ZnO-2 groups) or lipid- coated ZnO (LCZ1, LCZ-2 and LCZ-3 group) on the mRNA expression of antioxidant enzymes in jejunal and liver tissues are presented in Tables 3 and 4, respectively
Summary
Effects of dietary lipid-coated zinc on the antioxidant defense system in the small intestine and liver of piglets. The purpose of the study was to investigate the effects of lipid-wated ZnO (LCZ) and the level of LCZ compared with ordinary zinc oxide (ZnO) on antioxidant defense system in the intestine and liver of piglets. TIlUS, antioxidant minerals including Zn and selenium are widely used as feed additives due to their potential and beneficial effects on 'Corresponding author: lang, In-5urk, Animal Science and Biotechnolq,'Y, Gyeongnam National University of Science and Technolq,'Y, linju 52725, Korea. TIle most common sources used in animal diets are inorganic zinc oxide (2nO) and Zinc sulfate (211S0) as dietary supplemental 211 [15] Such a phannacologically high dose of inorganic Zn supplement, usually results in a very low bioavailability and subsequently could give rise to the environmental pollution of heavy metal, since up to 75 to 90% of supplemental lnO is not absorbed [15, 16]
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