Plant Weight Research Articles

Polybutylene adipate terephthalic acid (PBAT) biodegradable mulching films effectively affect the nutrition metabolism and growth of chewing cane compared to polyethylene mulching films

Plastic mulching films are vital materials in agriculture, contributing to the improvement of crop yield and quality but also causing environmental pollution. Using biodegradable mulching films (BM) can help mitigate this pollution. However, there is a lack of understanding regarding the impact of biodegradable mulching films on chewing cane growth compared to traditional polyethylene mulching films (PM). This study aims to compare the effects of biodegradable and non-biodegradable mulching films on chewing cane yield, soil enzymatic activities, root enzymatic activities, microbial abundance, and community diversity in rhizosphere soil. These experimental results showed that the tensile properties of BM gradually degraded over time. Both BM and PM significantly impacted the soil temperature, bulk density, and total porosity. The BM significantly affected the utilization of nitrate nitrogen, ammonium nitrogen, and organic matter by regulating the activities of urease, catalase, sucrose reductase, glutamine synthetase, and the gene expression of NIR2, GOGAT1, GOGAT2, GS1b, GS1c, NAR2, NRT2.1. Ascomycota (59%86%) was the dominant fungal phyla, and 383 fungal genera were detected in all samples, of which the main fungal genus was Chaetomium (4.95%34.25%). BM significantly increased the abundance of Rozellomycota and Geotrichum. BM increased the yield by 13.46%, doubled the root weight, and improved the plant weight by 26.25%. Our finding suggests that BM has a favorable regulation function in the utilization of nutrition and enriches some key factors that promote the growth of chewing cane. It advances understanding of the regulation mechanisms of biodegradable mulching films on the crops.

Development of Faba Bean Genotypes for Broomrape Tolerant and Foliar Diseases Resistance in F\(_2\) Factorial Crosses

Two field experiments were conducted at Sakha Agricultural Research Station, Kafr El-Sheikh, Egypt. The first experiment was in a naturally Orobanche- free field and the second one was in a naturally Orobanche-infested field. In each experiment sixteen F2 crosses and their parents were sown. The sixteen F2 crosses were produced by a 4 x 4 factorial mating design in F1 and selfed to give F2-crosses. The data were then analyzed using a line x tester design. The main objective of this study was to develop new promising faba bean genotypes that are able to produce high yield, resistance to foliar diseases and broomrape tolerance. Significant differences among all genotypes mean square were observed for all studied traits under both growth conditions. The parents, crosses and their interaction mean squares were detected for all traits in the two environments. The mean squares of lines were significant and/or highly significant for flowering date and 100-seed weight under both conditions, while number of pods per plant was significant under normal condition, in addition to the number of seeds per plant and broomrape dry weight per plant under infested broomrape condition were significant and highly significant, respectively. Mean squares of testers were significant for chocolate spot at the two conditions; rust disease, plant height, number of pods and seeds, seed yield per plant and 100-seed weight under normal condition. Lines x testers mean squares were significant for all traits in both conditions, except for number of branches and 100-seed weight. L1 and L4 and the parental testers T4 were considered more resistance parents to foliar diseases under both environments. The crosses; L2 x T3, L2 xT4, L3 x T2, L3 x T4 and L4 x T4 were considered resistant to foliar disease and had an earlier flowering date. The testers, T3 and T4 showed significantly the highest values of number of seeds per plant, seed yield per plant and 100-seed weight under both conditions, while the crosses; L3 x T4 and L4 x T4 had the highest values for seed yield per plant and 100-seed weight under both conditions. The parental L2 and T4 were considered good combiners for chocolate spot and rust diseases under both environmental conditions, while L1 and T4 performed as good combiner parents for number of seeds and seed yield per plant. The cross; L2x T3 performed as good specific combiner cross for foliar diseases under both conditions. The crosses; L2x T3, L3xT4, L3 x T1and L4 x T4 had highly significant (sij) values for the No. of seeds and seed yield per plant under infested soil condition. The phenotypic coefficient of variation (PCV) values ranged from 5.52% to 84.01% and genotypic coefficient of variation (GCV) values ranged from 3.18% to 79.21%. Broad-sense heritability (H) ranged from 72.98% to 99.29%, and narrow sense heritability (h2) ranged from 2.3% to 21.38%. Expected genetic advance (Ga) ranged from 0.03 to 0.71, and predicted genetic advance (Ga%) ranged from 8.21% to 10.86%.

Overexpression of vacuolar H+-pyrophosphatase from a recretohalophyte Reaumuria trigyna enhances vegetative growth and salt tolerance in transgenic Arabidopsis thaliana

Reaumuria trigyna, a wild and endangered salt-secreting small shrub, is distributed in arid and semi-arid areas of Inner Mongolia, China. An H+-pyrophosphatase gene (RtVP1) was isolated from R. trigyna according to transcriptomic data, which encoded a plasma membrane and tonoplast-localized protein. RtVP1 was quickly upregulated by NaCl and exogenous abscisic acid treatment and rescued the sucrose deficiency sensitive phenotype of the AtVP1 mutant (avp1). Transgenic Arabidopsis overexpressing RtVP1 exhibited a higher leaf area, plant height, fresh weight, root length, and soluble carbohydrate accumulation compared to the wild type (WT) under normal conditions. RtVP1 overexpression increased the seed germination rate and decreased the reduction rate of fresh weight, root length, and chlorophyll content in transgenic plants under salt stress. Catalase enzyme activity, proline content, relative water content, and soluble sugar content were significantly increased in transgenic Arabidopsis under salt stresses, but the malondialdehyde content was dramatically decreased. More K+ and less Na+ were accumulated in transgenic Arabidopsis leaves, resulting in a relatively lower Na+/K+ ratio. In transgenic Arabidopsis roots, K+ was unchanged, but Na+ and the Na+/K+ ratios were reduced compared to those in WT. More Na+ and K+ were accumulated in the intracellular of transgenic yeast, and the Na+/K+ ratio was significantly reduced compared to the control. These results showed that R. trigyna RtVP1 promotes the vegetative growth of plants, mainly by regulating carbohydrate metabolism, and confers salt tolerance in transgenic Arabidopsis by maintaining Na+/K+ homeostasis and enhancing the antioxidant and osmotic regulatory capacity. These results indicated that RtVP1 can serve as an important candidate gene for genetic improvement of crop yield and salt tolerance.

The First Information on the Low Molecular Metabolom Lobelia dortmanna (Campanulaceae, Magnoliophyta) Growing in the Northwest of the Russian Federation

The low molecular weight metabolome (LMWM) of Lobelia dortmanna L. (Campanulaceae, Magnoliophyta), a relic aquatic macrophyte listed in the Red Data Books of the Russian Federation and Republic of Belarus, was studied for the first time using the GC/MS technique. It is a macrophyte that grows in oligotrophic lakes in the northwest of the Vologda Region, Russia. Thirteen major chemicals were among the 127 volatile low molecular weight organic compounds (VOCs) discovered, which accounted for 77.23% of the overall VOC content (198.75 µg/g dry plant weight). The main components of LMWM were carboxylic acids and hydrocarbons. The presence of a large number of biologically active metabolites with a high concentration in L. dortmanna LMWM may indicate a significant effect of this plant on the hydrobiocenoses of the littoral zone of oligotrophic lakes. Further research into the LMWM of aquatic macrophytes in oligotrophic lakes, a valuable renewable resource, is needed to assess the background characteristics of the natural environment for more effective monitoring of the ecological state of water bodies in the Russian Federation's North-West and the rational use of their biological resources.

Fusarium oxysporum f. sp. apii Race 4 Threatening Celery Production in South Florida.

Fusarium wilt, caused by Fusarium oxysporum f. sp. apii (Foa), is a vascular disease affecting celery. This soilborne pathogen is classified into four distinct pathogenic races: 1, 2, 3, and 4. Notably, race 4 emerges as the most virulent, representing the latest evolutionary development of this pathogen, which was first reported in 2013 in California. In 2022, celery plants in South Florida exhibited typical Fusarium wilt symptoms, with the disease reaching a 100% incidence and causing yield losses ranging from 20 to 100%. Given the significance of celery as a vegetable crop and the severity of this outbreak, the primary objective of this study was to identify and characterize the causal agent of Fusarium wilt in South Florida. The second goal aimed to test the pathogenicity and virulence of the Fusarium isolates from Florida on celery and parsley plants. Using race-specific primers and dual-loci phylogenetic analyses, the isolates surveyed in this study were identified as Foa race 4. Pathogenicity assays in the greenhouse showed that the Foa race 4 isolate from celery induced disease not only on the two celery cultivars (Duda 30 and Duda 71) but also on two commonly cultivated parsley varieties (curly and Italian). Our study also revealed that the Foa race 4 significantly (P < 0.05) affected plant health attributes in all cultivars, including plant height, total plant weight, and root weight. Interestingly, the pathogen exhibited higher (P < 0.0001) virulence on parsley than celery based on vascular discoloration. These findings strongly indicate the urgency of comprehending and managing Fusarium wilt on celery and related crops. Furthermore, the ability of Foa race 4 to affect different plant species highlights a potential threat to agricultural production, emphasizing the need for proactive measures to mitigate the impact of this virulent pathogen.

Effect of the Root Endophytic Fungus Piriformospora indica on Strawberry Growth, Fruit Quality and Physiological Traits Under Elevated Electrical Conductivity

Strawberries are sensitive to abiotic stresses such as salinity, high levels of electrical conductivity, and nutrient imbalances. The mutualistic endophytic fungus Piriformospora indica has significant potential to be used in improving crop production under adverse conditions, with a large host range. However, greenhouse production requires novel tactics to improve the efficiency of saline water irrigation in areas with limited freshwater resources. This study was conducted at the National Research and Development Center for Sustainable Agriculture (Estidamah), KSA, to investigate the impact of P. indica colonization on the growth, photosynthesis traits, productivity, and fruit quality of Fragraria x ananassa Duch cv. Festival strawberry grown in pots and irrigated with two electrical conductivity (EC) nutrient solutions of 1.5 and 3.0 dS/m. The results showed that higher-EC-nutrient solution clearly reduced growth and early yield and improved vitamin C, TSS, and anthocyanin of strawberry in comparison to low-EC-nutrient solution. On the other hand, P. indica colonization significantly increased plant height, shoot fresh weight, root length, and early yield of plants grown under high-EC-nutrient solution with no impact on fruit quality. Obviously, the symbiosis between strawberry roots and P. indica enhanced chlorophyll content, photosynthetic rate, stomatal conductance, and transpiration rate, as well as antioxidant activity such as proline, malondialdehyde, catalase, superoxide dismutase, and peroxidase under higher-EC-nutrient solution. Our study indicated that P. indica might be used as a sustainable tool for strawberry production in arid and semiarid zones, to mitigate the negative impacts of higher-EC-nutrient solution.

The types of growing media and plant density on the growth and yield of kailan (Brassica oleracea L.) In a floating raft system hydroponic

This research aimed to know the effect of the type of growing media and population density on the growth and yield of kailan (Brassica oleracea L.) hydroponically. The research was conducted in the greenhouse of the integrated agricultural system area at Curug Serang Banten Province. This research used a Randomized Completely Block Design (RCBD) as factorial with two factors. The first factor was the type of growing media (J) which consisted of 3 levels, J1 (Rockwool), J2 (Zeolite), and J3 (Sponge). The second factor was population density (K), which consisted of 3 treatment levels, K1 (3 plants per tub), K2 (4 plants per tub), and K3 (5 plants per tub). The results showed that the type of growing media had a significant effect on plant height growth 7 Day After Plating (DAP) of (4.67cm), 14 DAP (11.58 cm), 21 DAP (17.09 cm), 28 DAP (22.29 cm), 35 DAP (37.57 cm), number of leaves 14 DAP (4.59), 28 HST (6.96), 35 DAP (8.59) and the root weight of your plants was 35 DAP (3.43gram). Population density treatment had a significant effect on plant height growth 28 DAP (22.26 cm), 35 DAP (37,37 cm) and there was an interaction between media type treatment and population density on plant height parameters 35 DAP.

Morphological, biochemical and genotoxic effects of non-ionizing radiation at 1800MHz and 2400MHz frequencies in Allium cepa L.

The frequent use of electronic devices in daily lives, predominantly reliant on non-ionizing radiation, has increased the prevalence of electromagnetic radiation (EMR) in natural environment. In light of this, effects of EMR at frequencies of 1800MHz and 2400MHz characterized by a power of 10.0 dBm (0.01 W), across varying exposure durations of 1h/day, 2h/day, 4h/day, 6h/day, and 8h/day for 7days, in Allium cepa L. were studied. The effects of the treatment on the morphological features (root length, fresh weight, and dry weight of roots) and biochemical characteristics (protein content and antioxidative enzymes, namely, ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST), guaiacol peroxidase (POD), catalase (CAT), and dehydroascorbate (DHAR)) were studied in roots and bulbs of Allium cepa L. Further genotoxicity for different exposure periods at both frequencies was also conducted. Prolonged exposure to electromagnetic radiation (EMR) at both frequencies was found to reduce root length, fresh weight, and dry weight of plant. Furthermore, significant effects were observed on protein content, indicating a reduction with prolonged exposure duration. Investigation into the activities of antioxidative enzymes such as APX, GR, GST, DHAR, CAT, SOD, and POD at a frequency of 1800MHz and 2400MHz in roots and bulbs demonstrated a significant enhancement in enzyme activity during 6h/day and 8h/day exposure periods. Additional investigation during genotoxicity studies demonstrated the induction of chromosomal aberrations in the root tip cells of the Allium cepa L. plant test system. The current study revealed the initiation of oxidative stress and genotoxicity resulting from long-term exposure to electromagnetic radiation in the studied plant test systems.

The effect of organic manure on the production of jute (Corchorus olitorius)

Field experiments to investigate the effect of different sources of organic manure (Swine manure (SM), Poultry manure (PM), Cattle manure (CM), Rabbit urine manure (RUM)) on production of Jute (Corchorus olitorius) was carried out. The experimental site was cleared and cultivated (tilled and fertilized). Treatments were laid out before planting at once, there was no re-application of treatment. Data were collected on growth and yield parameters (Foliage, Plant height and Plant fresh weight). There was a significant difference in the foliage of Corchorus olitorius treated with SM, PM, CM, and RUM when compared with the control group. The foliage of Corchorus olitorius with PM treatment is significantly higher (P&lt;0.05) compared with other organic manure treatment except for CM. Significant increase (P&lt;0.05) in the height of Corchorus olitorius was obvious in all the treatment group when compared with control. There is no significant difference (P&gt;0.05) in the height of jute across the organic manure treatment groups (SM, PM and RUM) but there is a significant increase in the plant height of PM when compared CM. SM, PM and RUM significantly increase the fresh weight of the plant when compared with the control and CM. There was a numerical increase in the plant fresh weight of PM when compared to other treatment groups. From this experimental study, PM consistently increased the number of leaves, the fresh plant weight and plant height significantly and it is recommended for Corchorus olitorius production.

Deciphering the Biocontrol Potential of Trichoderma asperellum (Tv1) Against Fusarium-Nematode Wilt Complex in Tomato.

The study assessed Trichoderma spp. as a biocontrol agent for managing wilt-nematode complex in tomato crops, aiming to mitigate yield losses. Fusarium sp. and Meloidogyne sp. were isolated from infected plant samples and confirmed molecularly and morphologically as Fusarium oxysporum f.sp. lycopersici and Meloidogyne incognita. Four Trichoderma spp. procured from the Department of Plant Pathology, Tamil Nadu Agricultural University, India were identified molecularly as Trichoderma asperellum (Tv1), Trichoderma asperelloides (Tasd1), Trichoderma harzianum (Th1), and Trichoderma koningiopsis (Tk1) utilizing ITS and TEF1 primer pairs. Among them, Tv1 effectively inhibited the mycelial growth of pathogen isolates. Furthermore, crude metabolite of Tv1 exhibited similar effects. The mortality rate of M. incognita J2s ranged from 90.48% to 100% after 24-72 h of incubation and inhibition percentage of egg hatching reached 90.20%. The shoot length, root length, fresh weight, and dry weight of the tomato plants treated with Tv1 conidia were increased. In a pot experiment, Tv1 treatment reduced disease incidence by 64.57%, comparable to carbendazim treatment (82.32%). Tv1-treated plants had fewer root galls, egg masses, and J2s per 100 g of roots than the inoculated-untreated control plants. The root-knot index (RKI) was significantly lower in plants treated with carbafuran (1.98 ± 0.047) and Tv1 (3.06 ± 0.086) than in control (4.47 ± 0.109). The bio-control efficiency of Tv1 against M. incognita was 21.04%, and the nematodes' reproductive factor (RF) declined to 0.53 in Tv1 treatment group. Based on the findings above, it was established that Tv1 effectively controlled nematode populations and wilt disease when applied in soil.

THE INHERITANCE OF SOME QUANTITATIVE CHARACTERS OF F2 GENERATION FOR COMMON WHEAT IN SULAIMANI-IRAQ

This study was aimed to testing 25 genotypes (5 parents + 10 diallel + 10 reciprocal crosses) using full Diallel mating design in winter season 2021-2022 for F2 generation of common wheat, in two locations. The first was in Qlyasan Agricultural Research Station, College of Agricultural Engineering Sciences; University of Sulaimani located (Lat 35° 34′; N, Long 45° 21′; E, 765 MASL), the second was in Koya Agricultural Station-Ministry of Agricultural (Lat 36° 04′; N, Long 44° 37′; E, 582 MASL). The results of the study confirmed that the mean squares of genotypes was highly significant for all characters in both locations, the same for the mean squares for gca with the exception of grain weight plant-1 in Qlyasan location which was only significant. The mean square for sca was highly significant for most characters at Qlyasan location, while at Koya location it was significant only for grain weight plant-1. The mean square for rca was significant for spike weight plant-1 at Qlyasan location and 1000-grain weight at Koya location and not significant for the other characters. The Diallel cross 1×4 at Qlyasan location and the reciprocal cross 2×1 at Koya location showed the best performance for grain weight plant-1 recording 58.083 and 40.941g plant-1 respectively. The magnitude of σ2sca was more than σ2gca for all characters in both locations indicating the importance of non-additive gene effects in controlling these characters. Heritability in broad sense for Diallel crosses due to grain weight plant-1 was found to be high in both locations, while for reciprocal crosses was low, and it was low for narrow sense for all crosses.

Efektivitas Pupuk Hayati dan Pupuk Kotoran Sapi Terhadap Pertumbuhan Serta Produksi Tanaman Pakcoy (Brassica rapa L.)

Pakcoy plants have an economic aspect and the business is worth developing or cultivating to meet consumer demand which is increasing over time. Increasing the production of pakcoy plants can be done by improving the fertility of the planting medium by using FertoMax biofertilizer and cow dung fertilizer. This research used a Randomized Block Design with 2 factors and 3 replications to obtain 48 research plots. Factor I of FertoMax Biological Fertilizer (S) which consists of 4 levels, namely: S0 = 0 ml/polybag, S1 = 5 ml/polybag, S2 = 10 ml/polybag, S3 = 15ml/polybag. Factor II Cow Manure (P) which consists of 4 levels, namely: P0 = 0 g/polybag P1 = 100 g/polybag, P2 = 200 g/polybag, P3= 300 g/polybag. The parameters observed in this study were plant height, number of leaves, leaf length, leaf width, tuber diameter, sample plant weight, plant weight per plot. The results of the study showed that the use of FertoMax biofertilizer with the best growth and production was at a dose of 15 ml/polybag while giving The best dose of cow dung fertilizer is 300 g/polybag. Keywords: pakcoy, fertomax biological fertilizer, cow manure

Genetic variability, heritability, and genetic advance estimates in selected groundnut genotypes (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) for agro-morphological and yield traits.

Groundnuts (Arachis hypogaea L.), is a crucial food and oil crop in the tropics, require genetic variability assessment for efficient breeding programs using appropriate parameters like genetic coefficient of variation, heritability estimates, association among yield related traits and genetic advance. This study aims to identify genotypes with desirable traits to improve yield and adaptability, contributing to better crop improvement strategies. The study used a randomized complete block design with three replications, consisting of fourteen selected genotypes. Quantitative traits were used to estimate genetic variability parameters, heritability, and genetic advance. The analysis of variance revealed significant differences (p &lt; 0.005) for all studied traits, indicating greater variability in genotypes. Characters exhibited different levels of variability, heritability, and genetic advance among the genotypes. The estimate of the broad-sense heritability ranged from 13.33% to 33.33% for canopy spread and days to maturity respectively. Low to high phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were recorded. The study found that traits such as number of branches, pods per plant, fresh and dry plant weight, kernel yield, pod yield, and shelling percentage showed high genotypic coefficient of variation, moderate broad sense heritability, and high genetic advance. These traits can be beneficial for groundnut improvement through effective phenotypic selection, resulting in high expected genetic gain. The study found that pod yield is significantly correlated with kernel yield, grain length, grain width, 100-seed weight, fresh and dry 100-pod weight, and number of pods per plant. Kernel yield is positively correlated with the number of branches per plant, pod per plant, fresh and dry 100-pod weight, 100-seed weight, grain length, and grain width. In addition, the first four principal components accounted for 90.37% of total variation in all morphological traits.

Evaluation of Ratoon Potential and Yield of Some Sorghum Varieties (Sorghum bicolor L)

Sorghum production needs to be increased to meet food or feed needs because sorghum grain has nutritional qualities that are comparable to other grains such as corn. One of the advantages of the sorghum plant is the growth of new shoots from the base of the harvested stem which produces new plants, ratoon, that grow until they can be harvested. This experiment examines the ratoon potential of 5 sorghum varieties, Bioguma, Samurai, Pahat, Suri4, and the local Gando Keta variety so that we can utilize ratoon technology on these sorghum varieties. The experiment was carried out in a greenhouse using a Completely Randomized Design. It can be confirmed from this experiment that the dry matter weight of the main plants is higher compared to the ratoon plants' dry matter weight, however, the yield of the ratoon was higher than that of the main plants. Seed size contributes to the higher yield of the ratoon plants. Among all the varieties tested, Suri4 and Pahat show the best ratoon potential. Gando Keta, the local variety, has the lowest grain yield of main and ratoon compared to the other varieties.

Sprouting and Growth of Garlic (Allium sativum L.) At Green Stage Under Various Nutrients.

Garlic (Allium sativum L.) is a vital crop, yet its growth and sprouting at the green stage can be significantly influenced by nutrient availability, which poses a challenge for optimizing yield and quality. The present research was carried out in 2022–2023 at the Nursery Department of Horticulture, Sindh Agriculture University Tandojam, to assess the sprouting and growth of garlic (Allium sativum L.) at green stage under various nutrients. The experimental trial was carried out in complete randomization (CRD) with three replications. Two varieties, a Chinese and a local (Desi) (Desi) variety, were grown in the pots. Macronutrients used as treatments included N1 (control). N2 = NPK+TE (trace elements) 2 g, L-1. N3 = Calcium Nitrate 0.5g, L-1 and N4 = NPK+TE 2g, L-1 + Calcium Nitrate 0.5g, L-1. The results of the present studies showed a statistically significant difference for treatments and nutrient combinations. Best results for all observations were noted in plants supplied with NPK+TE 2g L-1 that had maximum plant height 55.83 cm, leaves plant-1 6.83, leaf length 47.66 cm, leaf weight 8.96g, plant weight 13.20g, neck thickness 6.55mm, root weight 2.68g, and root depth 19.83cm. Fallow control plant height 41.00cm leaves plant-1 5.00, leaf length 31.00cm, leaves weight 4.73g, plant weight 7.61g, neck thickness 3.51mm, root weight 1.46g and root depth 12.00cm. In between varieties the Chinese had maximum plant height 54.66cm, leaves plant-1 7.00, leaf length 45.00cm, leaves weight 10.04g, plant weight 10.99g, neck thickness 8.26mm, root weight 2.81g and root depth 22.00cm. Based on nutrients, the Chinese variety of garlic performed better growth at the green stage than the local (Desi) variety of garlic. The findings of this study show that nutrient N2 = NPK+TE (Trace Elements) 2g L-1had better results for all parameters, and all results were statistically significant.

Studies on Genetic Divergence in Faba Bean (Vicia faba L.)

Background: The genetic divergence in faba bean genotypes is critical for breeding programs aiming to improve yield and other agronomic traits. Understanding the extent of genetic variability among different genotypes can guide the selection of superior varieties and enhance crop improvement efforts. This study was conducted to evaluate the genetic diversity among 47 faba bean genotypes grown under the specific agro-climatic conditions of Chhattisgarh. Methods: The experiment was conducted during the Rabi season of 2022-23 at the Hi-Tech-Nursery, Dau Kalyan Singh College of Agriculture and Research Station, Bhatapara, under Indira Gandhi Krishi Vishwavidyalaya, Chhattisgarh. A total of 47 faba bean genotypes were evaluated for genetic divergence using Mahalanobis D2 statistics and Tocher’s method. Ten quantitative characters, including marketable pod yield per plant and marketable pod weight appearance, were measured and analyzed. The genotypes were grouped into clusters based on their genetic distances. Result: The clustering pattern indicated that genetic diversity was independent of geographical diversity. Cluster II contained the highest number of genotypes (19), followed by Cluster I (13), while Cluster IV had the least (1). Among the ten quantitative characters studied, marketable pod yield per plant contributed most to genetic divergence, followed by marketable pod weight appearance. The maximum intra-cluster D2 value was observed in Cluster IV (477.35), followed by Cluster III (250.07). The highest inter-cluster D2 value was between Cluster I and Cluster IV (7917.34), followed by Cluster IV and Cluster V (6156.43).

Pengaruh Pembenah Tanah Cair dan Pupuk N, P, K terhadap Pertumbuhan Serta Hasil Tanaman Bawang Merah (Allium cepa, L.)

This research aims to determine the effects of humic acid application on soil pH, organic carbon (C-Organic), cation exchange capacity (CEC), as well as the growth and yield of shallots. The study was conducted at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University, Jatinangor, Sumedang, from November 2023 to February 2024. The experimental method used was a Randomized Complete Block Design (RCBD) with six treatments and five replications. The treatments were: A; (0.5 Soil Amendment and 1 Standard N, P, K), B; (1 Soil Amendment and 1 Standard N, P, K), C; (1.5 Soil Amendment and 1 Standard N, P, K), E; Without Standard N, P, K and Soil Amendment (Control), and F; (Standard N, P, K). The results showed that the combination of soil amendments and N, P, and K fertilizers significantly affected the parameters of soil pH, C-Organic, CEC, and plant height at 14, 28, 42, and 56 days after planting (DAP); stem diameter at 14, 28, 42, and 56 DAP; number of tillers at 14, 28, 42, and 56 DAP; and shallot plant weight.

The New Technology for In Vitro Culture with Induction of Nanobubbles (NBsN2 and NBsO2) in Cattleya sp.

The growth and development of orchids take a relatively long time, while the demand for high-quality orchids continues to increase significantly. Tissue culture is a technique of isolating plant parts in organs, tissues, and cells and then culturing these plants on artificial media in a sterile environment. Tissue culture techniques can reproduce plants in a relatively short time, with the same properties and quality, so efforts to optimize tissue culture with technology are the right solution, one of which is using nanogenerator technology that produces Nanobubbles (NBs). NBs are one of the nanotechnologies that are ?100 nm in size with induced gas needed by plants. This study aims to determine the effectiveness of growth and development from the induction of NBsN2 and NBsO2 in Cattleya sp. planlet in vitro culture. This research method uses in vitro culture with a Randomized Group Design. The results of the MsO treatment showed higher weight than other treatments, with a total plant weight value of 0.3550 g and a total plant height value of 1.3983 cm. Murashige-Skoog + NBsNitrogen (MsN) treatment showed higher root length than other treatments with a total value of 1.2367 cm. In the treatment of Ms, MsO, OJ and NJ, a live percentage of 83-100% was observed. The statistical test results indicate that NBs positively affect Cattleya sp. plantlets. This effect includes increasing plant weight, height, and root length and promoting new shoots growth. Additionally, nitrogen supplements play a role in colour changes, indicating the plant's health and photosynthetic efficiency. In the treatment with Induction O2, the initial colour changes from Strong Yellow Green (141 D) to Strong Yellowish Green (141 C), while in the treatment with Induction NBsN2, the initial colour changes from Strong Yellow Green (141 D) to Deep Yellow Green (141 B).

Effect of Soil Amendments on the Aggregate Stability, Biological Activity, and Yield of Canola (Brassica napus L.) in Degraded Soils

ABSTRACT Recycling organic waste and water treatment residues in nanoform is a promising technology in various farming systems, and its effect on aggregate stability, biological activity, and canola production in degraded soils is largely unknown. Hence, we evaluated the effect of nano-biochar (nB) and nano-water treatment residues (nWTR) at levels 50, 100, and 250 mg kg−1 as compared to soil without amendments on the aggregate stability, biological activity, and canola yield in degraded soil. The results showed that the structure coefficient (SC) values are higher when nB and nWTR are added at 100 mg kg−1 soil than at levels 50 and 250 mg kg−1. In our study, the high aggregate stability in the soil treated with nB and nWTR is likely due to their higher content of nutrients, organic carbon, clay, and CEC. The seed weight of the canola plant increased with increasing nB by more than 64%, while it decreased with increasing nWTR. Microbial biomass carbon (MBC) and the activity of dehydrogenase (DHA) and catalase (CLA) increased significantly in the treated pots with the addition of nB and nWTR at various rates. The results of the pot experiment recommended that the applications of nWTR and nB could be used as a promising strategy to improve soil quality and crop yield while also increasing the recycled efficiency of WTR and rice straw.

Daily Light Integral and Far-Red Radiation Influence Morphology and Quality of Liners and Subsequent Flowering and Development of Petunia in Controlled Greenhouses

Petunia stands as the top-selling bedding plant in the U.S., and improved lighting control in greenhouses holds the potential to reduce crop production time and optimize crop quality. This study investigated the impact of four distinct daily light integral (DLI) conditions with and without supplemental far-red (FR) radiation on the growth of petunia liners and subsequent development of finish plants. Two experiments were conducted in spring (9 April to 18 June 2021) and winter (28 October 2021 to 6 January 2022). Petunia cuttings were rooted in a common environment and then transferred to four greenhouse sections with different DLI treatments: 6, 9, 12, and 15 mol·m−2·d−1 for four weeks. Within each DLI condition, half of the plants were exposed to 28 μmol·m−2·s−1 supplemental FR radiation for 16 h daily (equivalent to 1.61 mol·m−2·d−1 light integral). The number of flower buds and open flowers were tracked daily. Representative liners were destructively harvested and evaluated after four weeks of lighting treatments. The remaining plants were transplanted and moved to a common DLI condition of 15 mol·m−2·d−1 for an additional three weeks before being destructively harvested and evaluated as finish plants. The primary finding reveals the promoting effect of DLI on flowering, branching, morphology, and biomass accumulation of petunia liners, with many effects persisting into the finish stage. A threshold DLI of 9 mol·m−2·d−1 was identified, as lower DLI (6 mol·m−2·d−1) resulted in extensive stem elongation, rendering the plants unmarketable. Higher DLI levels were found to be optimal in terms of flowering and morphology. Supplemental FR accelerated flowering by up to three days in the summer experiment and up to 12 days in the winter experiment. However, FR had limited impact on the number of flower buds and open flowers, branching, and shoot and root weight of the finish plants. Interactions between DLI and FR were observed on some parameters, whereby FR effects were more pronounced under lower DLI. Overall, both higher DLI and supplemental FR exhibited beneficial effects, but DLI had a more pronounced effect. Thus, DLI during petunia liner production appears more important than adding FR. This study well simulated the commercial propagation and production of petunia plants, providing practical insights for decision-making regarding lighting strategies.