Plant Weight Research Articles

Growth and Yield of Lentil as Influenced by Zinc and Boron Management

A field experiment was conducted at Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh, during the period from November 2021 to March 2022 to evaluate the influence of micronutrients management on growth and yield of two lentil varieties viz., BARI Masur-8 (V1) and Binamasur-8 (V2) and 6 micronutrients management (no fertilizer - F1, recommended fertilizer dose (RFD) (40-90-40-55 Kg ha−1 of urea-TSP-MoP-Gypsum) with 10 Kg ha−1 ZnSO4 as basal - F2, RFD with zinc (Zn) as foliar spray - F3, RFD with Zn and boron (B) as foliar spray - F4, 50% RFD + 50% cow dung (5 t ha−1) - F5 and 50% RFD + 50% cow dung + Zn and B as foliar spray - F6). No significant effect had found for variety except 1000-seed weight but micronutrients management along with interactions significantly effects on all studied parameters of lentil. The higher 1000-seed weight (34.16 g) was recorded in BARI Masur-8. The highest plant height (37.09 cm), number of branches plant−1 (7), dry weight of root plant−1 (159.17 mg), dry weight of shoot plant−1 (3319.33 mg) and number of pods plant−1 (62) was observed at F6 treatment. The highest 1000-seed weight (31.68 g) were recorded at F2 treatment. The highest weight of nodules plant−1 (10.33 mg at 60 DAS), seed yield (2.62 t ha−1), straw yield (2.12 t ha−1) and biological yield (4.74 t ha−1) were found at F3 treatment. The highest plant height (38.45 cm), number of branches plant−1 (7), number of pods plant−1 (63), seed yield (2.91 t ha−1) and harvest index (58.65%) were recorded at V2F6 whereas the highest weight of nodules plant-1 (26 mg), straw yield (2.22 t ha−1) and biological yield (5.02 t ha−1) were found at V1F3 but the highest 1000-seed weight (34.87 g) at V1F1. The highest number of seeds pod−1 (1.83) was found at V2F1. So, two varieties showed similar performance on yield. Foliar application of Zn has the potentiality to increase seed yield. The variety Binamasur-8 with 50% RFD + 50% cow dung + Zn and B (F6) as foliar spray could bring maximum yield (2.91 t ha−1) of lentil followed by treatment F2, F3, and F4. Bangladesh Agron. J. 2024, 26(2): 34-40

Influence of Different Cow Manure Application Rates on the Growth, Yield and Indigo Blue Color of Indigofera suffruticosa Mill

Background: Indigo is a key ingredient in the natural dyeing of fabrics. However, the conventional use of chemical fertilizers in indigo cultivation has led to an increase in production costs and soil degradation. Low-fertility soils further compromise the growth and yield of indigo plants. To address these challenges, employing manure is recognized as a sustainable approach for indigo cultivation. The use of manure enhances soil nutrient levels, improves soil structure and mitigates production costs. In this study, our objectives were to compare different rates of cow manure in relation to indigo growth, yield and the effect of cow manure rate on the value of indigo blue color. Methods: The experiments were carried out at the Plant Science Field from June to October 2022. Employing a randomized complete block design (RCBD), the study featured four treatments with four replications each. The treatments included: Treatment 1: no fertilizer (control); Treatment 2: cow manure at a rate of 3,125 kg/ha; Treatment 3: cow manure at a rate of 6,250 kg/ha; and Treatment 4: cow manure at a rate of 9,375 kg/ha. Data collection included plant height, canopy width, number of branches, plant weight, indigo blue weight and indigo blue color value. The CIE color system (Commission International de l’Éclairage) determined the color value. Result: The application of cow manure at a rate of 9,375 kg/ha resulted in the highest plant height and canopy width, with the corresponding number of branches of 150.73 cm, 46.45 cm and 31.73 cm, respectively. As compared to the other treatments, these values were statistically different. The color that was associated with the indigo finished product was dark blue and the yield; plant weight and indigo blue weight were recorded as 15,663.44 kg/ha and 2,540.44 kg/ha, respectively. The color value of indigo blue showed that the L* values of 24.94, a* -9.32, b* -2.44 and C* 9.63 were not significant but h* 194.66 was statistically significant.

Non-thermal plasma enhances growth and salinity tolerance of bok choy (Brassica rapa subsp. chinensis) in hydroponic culture.

In this study, we aimed to examine the growth, physiological and biochemical status, and responses to salinity stress of bok choy (Brassica rapa subsp. chinensis) cultivated in a hydroponic system with a plasma-treated solution. Plasma gas generated using a cylindrical dielectric barrier discharge or air (control) was injected into Hoagland nutrient solution once a week for different durations (0, 5, and 10 min). After 4 weeks, the length of the shoots and roots, number of leaves, and dry weight of bok choy plants significantly increased in individuals grown with Hoagland solution treated with plasma gas for 10 min. An increase in dry weight of individual plants of approximately 80.5% was observed in plants in the plasma-treated group compared to those in a control group. The levels of chlorophyll, total soluble proteins, and nitrogen uptake, and transcription of genes related to salinity stress tolerance-WRKY2, HHP3, and ABI1- were also significantly elevated in bok choy grown with plasma treated Hoagland solution. Moreover, when exposed to 20 mM NaCl, plant length and leaf number were significantly increased, in the group grown with Hoagland solution treated with plasma gas for 10 min. Level of H2O2 was significantly elevated in the treated nutrient solutions. In plants grown with the treated nutrient solution, intracellular NO was highly detected in the cell division and elongation zone of roots. Our findings suggest that plasma treatment of nutrient solutions in hydroponic culture systems may improve the growth, physiological and biochemical status, and tolerance to salinity stress in plants, and a crucial role of H2O2 generated in the treated nutrient solutions may play in this improvement.

Effects of soil amendments and coverings on the kenaf yield and soil physicochemical properties in saline-alkali land

In order to investigate the effects of various treatments on the growth of kenaf in saline soil, this study employed a comprehensive experimental design incorporating a dual approach of soil amendment and mulching techniques. Soil treatment of saline and alkaline land, as well as biological material mulching treatment after sowing kenaf, were conducted. The growth dynamics of kenaf were assessed before the mid-term investigation, and the harvest period was examined. Soil physicochemical properties were measured and analysed at different periods. The results show that in the analysis of red sesame yield traits, the differences mainly appeared between soil treatments. There is no significant difference between the different mulching treatments within each soil treatment. In the mid-term study, significant differences were observed in kenaf plant height. Plant height was the most affected by soil conditioners, while other yield traits did not show significant variations. During harvest, only stem thickness and dry weight of a single plant did not show significant differences, whereas other yield traits did. The application of soil conditioners resulted in a 58.15% increase in dry hull yield compared to organic fertilizers and a 22.66% increase compared to regular fertilizers. Soil conditioners also led to higher levels of effective phosphorus, quick-acting potassium, organic matter, and total nitrogen throughout the kenaf growth period compared to organic and regular fertilizers. This is beneficial for enhancing soil quality and reducing soil alkalinity. Combined with the results of kenaf yield and soil physical and chemical properties analysis, it has been proven that the application of soil conditioner has the best effect, followed by the application of compound fertilizer, with organic fertilizer treatment being the least effective.

Recent Advances in the Extraction and Characterization of Bioactive Compounds from Corn By-Products.

Maize comes in a variety of colors, including white, yellow, red, blue, and purple, which is due to the presence of phytochemicals such as carotenoids, anthocyanins, flavonoids, phytosterols, and some hydroxycinnamic acid derivatives. In Mexico, maize is primarily grown for human consumption; however, maize residues comprise 51-58% of the total maize plant weight (stalks, leaves, ears, and husks) and are mainly used as livestock feed. These residues contain numerous bioactive compounds that interest the industry for their potential health benefits in preventing or treating degenerative diseases. This review explores the current knowledge and highlights key aspects related to the extraction methods and different techniques for identifying the bioactive compounds found in maize by-products.

Multi-scale hybrid modeling of plant growth in response to environmental conditions and soil nutrients availability

Crop modeling plays a crucial role in agriculture, aiding our understanding and prediction of crop growth and yield in diverse environmental conditions. This study aims to develop a comprehensive mathematical model describing plant growth in response to environmental conditions and soil nutrient availability. To achieve this, we relied on a field experiment with lettuce plants under varying environmental conditions. Employing growth models such as logistic, Gompertz, Aikman & Scaife, and Scaife, Cox & Morris, we assessed the influence of time, day-degrees, and effective-day-degrees across different plant densities and during distinct periods throughout the year. In general, describing plant growth in terms of day-degrees or effective-day-degrees yielded an improved model fit and more precise estimations of growth parameters. As a result, we described the growth of plant length in terms of effective-day-degrees instead of time in the equations of the Bessonov-Volpert system. Additionally, we modified the equation describing plant length growth using previously fitted functions. By incorporating these adjustments, we characterized the one-dimensional growth of plant weight under varying environmental conditions without branching, using the Bessonov-Volpert model. This study contributes valuable insights into crop modeling techniques, refining our understanding of optimizing plant growth under different environmental conditions.

Assessment of Combining Ability and Gene Action in F1 Generation for Quantitative and Qualitative Traits of Pigeonpea [Cajanus cajan (L). Millsp.

To estimate combining ability and nature of gene action, twenty four pigeonpea crosses were developed from ten parents in Line × Tester fashion during Kharif 2022-23 and were evaluated in Randomized Block Design with three replications during Kharif 2023-24. The estimates of σ2gca were lower than the corresponding σ2sca for all the twelve characters studied indicating the predominance of non-additive gene action. Among the parents, the line GJP 1915 was found to be good general combiner for seed yield per plant and its yield contributing characters like plant height, number of primary branches per plant and pod length. The tester GJP 1820 was also good general combiner for seed yield per plant and its component traits like plant height, number of primary branches per plant, number of secondary branches per plant, number of pods per plant and 100-seed weight. The cross BDN 2013-41 × UPAS 120 had registered the highest sca effect for seed yield per plant. The sca effect in this cross combination was also accompanied by significant and desirable standard heterosis and high per se performance for days to maturity, reproductive phase duration, number of secondary branches per plant, number of pods per plant and number of seeds per pod and significant and desirable heterobeltiosis for days to maturity, reproductive phase duration, number of primary branches per plant, number of pods per plant and 100-seed weight. The cross GJP 1915 × BLACKTUR had registered significant and positive sca effect for seed yield per plant which was also accompanied by highest per se performance for pod length. These cross combinations can be potentially utilized in future breeding programmes for exploitation of hybrid vigour.

Effectiveness of some novel fungicides against the sheath blight pathogen Rhizoctonia solani in rice under in vitro and in vivo conditions

Sheath blight of rice, caused by Rhizoctonia solani, is one of the most aggressive and damaging diseases in rice cultivation, leading to significant crop losses. Consequently, chemical fungicides are the most effective method to inhibit R. solani infection and control this disease. In the present study, R. solani isolate was obtained from infected rice plants, and its pathogenicity was confirmed using Koch’s postulates on 20-day-old rice seedlings at three different inoculum levels. The lowest percent disease index and disease incidence were recorded with the 1 ml pathogen inoculum suspension, while the 2 ml and 3 ml suspensions resulted in 100% disease incidence, significantly affecting plant parameters. Furthermore, five different fungicides, Duphetar Plus, Evito, Nativo, Ridomil Gold, and Shincar, were evaluated at three concentrations (10, 100, and 1000 ppm) against R. solani using poisoned food method. The results revealed that two fungicides, Shincar and Nativo, resulted in maximum mycelial growth inhibition of R. solani, whereas the other fungicides failed to inhibit the mycelial growth of the pathogen at all concentrations. Shincar and Nativo were further tested in a greenhouse setting to control pathogen infection in rice plants. These fungicides showed excellent results, reducing R. solani infection and minimizing disease incidence in treated rice plants compared to the control. The highest root length, shoot length, and plant weight were observed with Shincar, followed by Nativo. It is suggested that the use of these fungicides may reduce sheath blight disease incidence, effectively control pathogen infection, and improve plant growth parameters in field conditions.

Effects of foliar and root application of Zn and Fe bio-nanofertilizers on the glucosinolate, Zn and Fe contents of Pak choi (Brassica rapa Subsp. Chinensis) grown under hydroponic and pot cultivation

Pak choi is a rich source of minerals and health beneficial compounds such as glucosinolates (GSLs). This study is focused on implementing sustainable agronomic approaches to optimize pre-harvest cultivation and improve plant growth and nutritional value. Therefore, the aim of this work was to evaluate the effect of foliar and root fertilization of algae capped ZnO and γ-Fe2O3 nanoparticles on the growth and content of GSLs, Zn and Fe of Pak choi grown under hydroponic floating system and pot conditions. The foliar application of ZnO (77 nm) and γ-Fe2O3 (68 nm) nanoparticles functionalized with an Arthrospira platensis extract increased fresh weight (39.71 %), plant height (42.77 %), leaves number (26.47 %), root length (33.33 %), and Zn (10-fold) and Fe (2-fold) contents in hydroponic plants. The same treatment also improved plant fresh weight (31.73 %), and Zn (6-fold) and Fe (2-fold) in pot cultivated Pak choi. Six GSLs were detected, which were also enhanced by the joint application of nanoparticles and microalgae extract. The results revealed a high synergy between the ZnO- and γ-Fe2O3 nanoparticles and the A. platensis extract to increase plant growth and Zn and Fe micronutrients and glucosinolates in Pak choi grown under hydroponic and pot systems.

Excess supply of sulfur mitigates thallium toxicity to rice (Oryza sativa L.) growth in hydroponic experiment

Sulfur (S) is an essential element for the growth of rice plants (Oryza sativa L.), crucial for enhancing crop yield and grain quality. However, its potential in mitigating thallium (Tl) toxicity in rice remains unclear. In this study, a hydroponic experiment was performed to investigate the effects of low, medium and high S application levels (LS, MS, HS) on Tl accumulation in rice at three Tl exposure levels (0, 0.5 and 1 mg·L−1). Our findings reveal that the exogenous S application could alleviate Tl toxicity, enhancing fresh weight and shoot length of rice plant. Additionally, HS (HS, SO42− content was 387.84 mg·L−1) group significantly increased chlorophyll and glutathione (GSH) content by 6.46 to 21.38 % and 2.15 to 7.31 % respectively, while reducing malondialdehyde (MDA) levels by 17.43 to 28.48 %, compared to MS (MS, SO42− content was 193.41 mg·L−1) group. Fe content in rice roots and iron plaque consistently increased with S provision under Tl-free and Tl-contaminated conditions. In Tl exposure environment, HS and LS (LS, SO42− content was 1.02 mg·L−1) groups exhibited significant differences in Fe contents and iron plaque in rice root. Moreover, in Tl exposure environment, S application reduced Tl concentration in iron plaque, root, and shoot, HS treatment showed Tl content reduction from 16.29 % to 25.89 %, compared to LS treatment. Our findings underscore the potential of S application in hydroponic environment to promote rice growth and mitigate Tl accumulation, offering insights for developing effective Tl remediation strategies by using S-contained fertilizers.

The growth‐promoting effects of Bacillus amyloliquefaciens W82T‐44 on soybean and its biocontrol potential against soybean Phytophthora root rot

AbstractThe most devastating soilborne disease of soybean is Phytophthora root rot (PRR) caused by Phytophthora sojae. Biological control has emerged as an effective method of reducing soilborne diseases. The present study isolated the bacterial strain W82T‐44 from the rhizosphere soil of a resistant soybean variety Williams 82. This strain was identified as Bacillus amyloliquefaciens and exhibited a 92.4% inhibition of mycelial growth of P. sojae. W82T‐44 produced cellulase, siderophore and protease and significantly promoted soybean growth. W82T‐44 treatment, compared to the negative control, was found to significantly (p < 0.05) increase soybean plant height (22.34 vs. 18.47 cm), fresh weight of the whole plant (2.57 vs. 2.05 g), dry weight (0.43 vs. 0.32 g), total root length (266.80 vs. 182.53 cm), root area (27.10 vs. 17.84 cm2) and root volume (1.88 vs. 1.16 cm3). The fermentation filtrate of W82T‐44 had significant inhibitory effects on mycelial growth, oospore formation and cyst germination in P. sojae and additionally promoted zoospore encystment. W82T‐44 possessed genes involved in lipopeptide synthesis, including bacilysin, surfactin, fengycin and bacillomycin. Moreover, it significantly upregulated pathogenesis‐related genes (β‐1,3‐glucanase, nonexpressor of pathogenesis‐related gene 1, chitinase) and genes encoding defence enzymes (polyphenol oxidase, phenylalanine ammonia‐lyase, peroxidase) in soybean roots (p < 0.05). The PRR disease index of soybean treated with the W82T‐44 strain was significantly lower (11.67) than the negative control (40.00; p < 0.05). The present study indicates the potential of B. amyloliquefaciens W82T‐44 as a biological control agent against PRR from various perspectives.

β values obtained by linear regression models of morpho-physiological and biochemical variables as novel drought stress estimators in Capsicum annuum varieties

Capsicum annuum varieties are highly sensitive to drought. Under water stress conditions, these can show yield losses of up to 70 %. Due to the above, this work proposes a novel approach to obtain estimators of drought stress based on linear regression models for morpho-physiological and biochemical variables in jalapeño pepper (C. annuum cv. jalapeno M), bell pepper (C. annuum cv. california wonder), and serrano pepper (C. annnuum cv. serrano tampiqueno). Jalapeno pepper plants were grown for 69 days under permanent water deficit conditions at 40, 60, 80 % and 100 % of field capacity (FC) (100 % FC as control). Throughout the crop cycle, we monitored the plant's height and weight, basal stem diameter, transpiration, photosynthesis, stomatal conductance, NDVI, and proline. This monitoring allowed us to obtain linear regression models from the accumulated values for these variables, from which the slope values (β) were used as estimators of drought stress using the interval estimation method, in the same way, this method was used to estimate water status in bell pepper and serrano pepper. For bell pepper, drought levels of 40, 60, 80 and 100 % FC were imposed for 12 days and serrano pepper 60 and 100 % FC for 63 days. The results showed that this method can be used to estimate drought stress in jalapeno pepper for all the irrigation levels through photosynthesis and NDVI and can be applied for bell pepper and serrano pepper using stem diameter and plant height, and in the case of serrano pepper, NDVI showed adequate results. Also, this work establishes the relationship between the jalapeno pepper responses (morpho-physiological and biochemical) to drought stress during vegetative, flowering, and fruiting stages through a Principal Component Analysis (PCA). The PCA found that interaction among morphological, physiological, and biochemical responses change concerning the phenological stage of the plant. The results suggested several direct and inverse relationships between the variables and showed that drought can be described by stomatal conductance during any phenological stage of the crop. In parallel, the proline content, NDVI and plant height can also describe drought stress during the vegetative and flowering stages. This research is the first to apply this methodology to drought stress estimation in jalapeno, bell pepper, and serrano pepper cultivation. The results could significantly contribute to precision agriculture, sensor development, and water management.

Genome-wide transcriptome and gene family analysis reveal candidate genes associated with potassium uptake of maize colonized by arbuscular mycorrhizal fungi

BackgroundPotassium (K) is an essential nutrient for plant growth and development. Maize (Zea mays) is a widely planted crops in the world and requires a huge amount of K fertilizer. Arbuscular mycorrhizal fungi (AMF) are closely related to the K uptake of maize. Genetic improvement of maize K utilization efficiency will require elucidating the molecular mechanisms of maize K uptake through the mycorrhizal pathway. Here, we employed transcriptome and gene family analysis to elucidate the mechanism influencing the K uptake and utilization efficiency of mycorrhizal maize.Methods and resultsThe transcriptomes of maize were studied with and without AMF inoculation and under different K conditions. AM symbiosis increased the K concentration and dry weight of maize plants. RNA sequencing revealed that genes associated with the activity of the apoplast and nutrient reservoir were significantly enriched in mycorrhizal roots under low-K conditions but not under high-K conditions. Weighted gene correlation network analysis revealed that three modules were strongly correlated with K content. Twenty-one hub genes enriched in pathways associated with glycerophospholipid metabolism, glycerolipid metabolism, starch and sucrose metabolism, and anthocyanin biosynthesis were further identified. In general, these hub genes were upregulated in AMF-colonized roots under low-K conditions. Additionally, the members of 14 gene families associated with K obtain were identified (ARF: 38, ILK: 4, RBOH: 12, RUPO: 20, MAPKK: 89, CBL: 14, CIPK: 44, CPK: 40, PIN: 10, MYB: 174, NPF: 79, KT: 19, HAK/HKT/KUP: 38, and CPA: 8) from maize. The transcript levels of these genes showed that 92 genes (ARF:6, CBL:5, CIPK:13, CPK:2, HAK/HKT/KUP:7, PIN:2, MYB:26, NPF:16, RBOH:1, MAPKK:12 and RUPO:2) were upregulated with AM symbiosis under low-K conditions.ConclusionsThis study indicated that AMF increase the resistance of maize to low-K stress by regulating K uptake at the gene transcription level. Our findings provide a genome-level resource for the functional assignment of genes regulated by K treatment and AM symbiosis in K uptake-related gene families in maize. This may contribute to elucidate the molecular mechanisms of maize response to low K stress with AMF inoculation, and provided a theoretical basis for AMF application in the crop field.

Assessing the Efficacy of Cyanobacterial Strains as Oryza sativa Growth Biostimulants in Saline Environments.

Soil salinity, which affects plant photosynthesis mechanisms, significantly limits plant productivity. Soil microorganisms, including cyanobacteria, can synthesize various exometabolites that contribute to plant growth and development in several ways. These microorganisms can increase plant tolerance to salt stress by secreting various phytoprotectants; therefore, it is highly relevant to study soil microorganisms adapted to high salinity and investigate their potential to increase plant resistance to salt stress. This study evaluated the antioxidant activity of four cyanobacterial strains: Spirulina platensis Calu-532, Nostoc sp. J-14, Trichormus variabilis K-31, and Oscillatoria brevis SH-12. Among these, Nostoc sp. J-14 presented the highest antioxidant activity. Their growth-stimulating effects under saline conditions were also assessed under laboratory conditions. These results indicate that Nostoc sp. J-14 and T. variabilis K-31 show significant promise in mitigating the harmful effects of salinity on plant size and weight. Both strains notably enhanced the growth of Oryza sativa plants under saline conditions, suggesting their potential as biostimulants to improve crop productivity in saline environments. This research underscores the importance of understanding the mechanisms by which cyanobacteria increase plant tolerance to salt stress, paving the way for sustainable agricultural practices in saline areas.

Characterization of Yield, Physiological and Biochemical Responses in Chickpea Genotypes under Contrasting Water Availability

Chickpeas are a rich source of protein and essential nutrients, making them a staple in many diets worldwide. They are also drought-tolerant, which makes them a valuable crop for improving food security in arid regions. Thirty chickpea genotypes were evaluated under irrigated and rainfed condition separately Randomized Complete Block Design (RCBD) with three replications at Post Graduate Institute Farm, MPKV Rahuri to study yield, physiological and biochemical response for drought tolerance in Rabi 2022-2023. Analysis of variance observed significant difference among studied genotypes in irrigated and rainfed condition for all the characters studied. Genetic variation exists in diverse chickpea genotypes for bio-physiological, morphological and yield attributing characters which can be explored for genetic improvement. As expected, water stress adversely affected the physiological, biochemical and yield responses of all the chickpea genotypes evaluated. The performance of genotypes Phule G-16318 and Phule G-1420-13-6 was observed to be stable for most of the characters such as Chlorophyll index, membrane stability index, relative water content, photosynthesis rate, proline content under moisture stress condition. Similarly, both the genotypes with minimal reductions in seed yield, 100 seed weight, pod number and biomass plant-1 under rainfed condition, can serve as donors for drought tolerance in chickpea improvement programs.

Effects of Native Rhizobia on Soybean [Glycine max (L.) Merrill] Production and Soil Properties in Daloa, Center-West of Côte d’Ivoire

The field experiment study was conducted at the experimental station of Jean Lorougnon Guede University in Daloa to evaluate the potential effects of native rhizobia strains on soybean production and soils properties. The experiment was layout in a randomized complete block design with three replications. The treatments include seven native strains, one exotic strain (Bradyrhizobium japonicum IRAT FA3), one synthetic fertilizer NPK 12-22-22 formulation and one negative control (no fertilizer, no inoculation). Agronomic parameters (plant height, plant dry biomass, nodule number plant-1, pod number plant-1, pod weight plant-1, seed yield ha-1) and soil parameters (pH, total nitrogen, orgnanic carbon, organic matter and available phosphorus) were measured. Results showed that all tested native rhizobia enhanced nodules number, plant growth and soybean yield as compared to negative control. Among these native rhizobia, RSC119 and RSC504 induced more nodules than the introduced strain B. japonicum IRAT FA3. RSC119, RSC309 and RSC508 produced more pods and seed yield than the introduced strain B. japonicum IRAT FA3 and the synthetic fertilizer NPK 12-22-22. RSC508 induced the highest pods number plant-1 (102 pods) and seed yield (4.2 t.ha-1). Moreover, these local bacteria had positive effects on soil properties following the soybean’s harvest. Among these bacteria, RSC119 enhanced mostly total nitrogen, organic matter and available phosphorus, then RSC508 significantly reduced soil acidity. This study therefore suggests that local rhizobia are effectiveness and could be use as inoculum to improve soybean productivity and soil properties restoration.

Interactive Effects of Boron and Molybdenum on Nodulation, Growth and Yield of Cowpea under South Gujarat Condition

Background: Cowpea seeds are nutritious component of human diet and being a legume crop, it also improves the soil fertility by increasing the amount of nitrogen in the soil through symbiotic nitrogen fixation. To study the effect of boron and molybdenum on nodulation, growth and yield of cowpea the field experiment was conducted at the Regional Horticultural Research Station, Navsari Agricultural University, Navsari, Gujarat, India, during summer season of 2019 and 2021. Methods: The experiment was carried out in a factorial randomized block design with 3 replications. Factor-1 consisted of four levels of seed treatments with molybdenum at 2 mg/l (M1), 4 mg/l (M2), 6 mg/l (M3) for 24 hours prior to sowing including control (M0), where as the second factor was consisted of four levels of boron (B) spray at 2 mg/l (B1), 4 mg/l (B2), 6 mg/l (B3) and control at 30 DAS, 45 DAS and 60 DAS with control. Boron was applied in the form of Boric acid and Molybdenum as ammonium molybdate. Result: The treatment combination of seed treatment of molybdenum @ 2 mg/ l with foliar spray of boron @ 4 mg/ l at 30, 45 and 60 DAS (M1B2) has significantly influenced yield parameters and registered maximum number of clusters per plant (6.50), green pod yield per plant (0.153 kg), green pod yield per net plot (3.98 kg), green pod yield per hectare (12.27 tonne). Seed treatment with different levels of molybdenum has significantly influenced nodulation in cowpea and recorded maximum number of nodules per plant (31.69 at final harvest) and fresh nodule weight (0.230 g/plant) in seed treatment of molybdenum @ 2 mg/ l (M2) among all the treatments at final harvest during all the years of study, including pooled analysis.

Genes of photoperiod sensitivity and early maturity E1-E4: dynamics of soybean growth in different daylength conditions

Background. Morphometric indicators are crucial for evaluating the development and productivity of soybeans. They are influenced by genetic and environmental factors. The use of nearly isogenic soybean lines is a convenient model for determining the impact of early maturity genes and daylength on growth indicators. The aim of this study was to determine the influence of different daylengths and early maturity genes on soybean morphometric indicators under conditions of the temperate zone (at the latitude of Kharkiv – 50° N). Materials and Methods. The study involved nearly isogenic soybean lines of the “Clark” cultivar with varying sensitivity to daylength. The research covers the results of field experiments over three seasons using different soybean lines. After reaching the V3 stage, some plants were subjected to short-day conditions for 14 days. Morphological indicators of ten plants per experimental variant were determined: plant height, dry weight, leaf number, and leaf surface area of soybean plants. Morphometric measurements were taken on the day of the beginning of different daylength treatments and on days 7, 14 and 21. The study results are presented as the mean values of the investigated parameters (plant height, dry weight, number of leaves and leaf surface area of soybean plants). Results. It was shown that under long-day conditions, dominant alleles of the E1 and E3 genes increased the dry weight of plants, while the dominant allele E2 increased plant height. The dominant allele E4 had no significant effect on plant height and weight indicators of soybean plants. Plants with dominant alleles of genes E1-E4 under long-day conditions had smaller leaf area compared to lines with recessive alleles of these genes. Conclusions. The obtained results on the relationships between genetic and environmental factors in influencing soybean plant height, weight, and leaf area can be useful in improving soybean yield and selecting cultivars that will be productive in high latitude conditions.

PENGEMBANGAN CABAI KATOKKON (Capsicum annuum L. var. sinensis) DI LUAR HABITAT ASLINYA

This research aims to develop Katokkon chilies outside their natural habitat with different environmental conditions. Increase soil fertility levels in areas with low fertility levels by adding manure and NPK fertilizer. The study used a two-factor randomized block design (RBD): NPK fertilizer and cow manure. NPK fertilizer consisted of 2 grams, 4 grams, 6 grams per 10 kg of soil, and control (without NPK fertilizer). Cow manure treatment consisted of 0.5 kg, 1 kg, and 1.5 kg per 10 kg of soil and without cow manure (control). Each treatment was repeated 3 times and consisted of 3 plants for each observation unit. The research results show that developing katokkon chili plants outside their natural habitat is still possible. Providing 0.5 kg of cow manure had the best effect on the parameters of plant height, fruit weight, and production of Katokkon chili plants. Adding 2 g NPK fertilizer provided the best growth in terms of plant height, fruit weight, and production parameters. However, the addition of 6 g NPK fertilizer affected the productive branch parameters of Katokkon chili plants. There was a real interaction between 0.5 kg cow manure and the addition of 2 g NPK fertilizer on the parameters of plant height, fruit weight per plant, and production of Katokkon chili plants

Phosphate solubilization potential of PSB: an advance approach to enhance phosphorous availability for phytostimulation.

Rhizosphere engineering approach is considered a quantum leap in plant sciences. The current study focused on investigating rhizobacterial efficiency to mobilize bioavailable phosphate from insoluble-phosphate source. Four efficient phosphate-solubilizing bacterial isolates, i.e., Pseudomonas songnenensis (GR3), Stutzerimonas stutzeri (HH2), Bacillus bingmayongensis (KH3), and Achromobacter aegrifaciens (MH1) were selected for the current study. Interactions between various physiological parameters and phosphate solubilization efficiency of isolates revealed that glucose significantly facilitated phosphorus solubilization at 37 ℃, with media having pH 7 and 0.5% phosphorous. Additionally, positive correlation among P-solubilization potential, acids produced, and pH was observed. Plant microbe-interaction analysis was performed to evaluate the efficiency of these bacterial isolates on various morpho-physiological responses of Zea mays L. For this purpose, various concentrations of tricalcium phosphate (TCP) (0, 10, 20, 30, 40, and 50mM) were applied to plants in the presence and absence of bacterial isolates. The results showed that lower phosphate levels (10 and 20mM) trigger shoot development and improve plant weight and leaf formation whereas higher phosphate concentrations (30mM and above) stimulated the development of longer root system. The bacterial isolates, KH3 and HH2, were observed as efficient phosphate-solubilizing bacteria (PSB) that positively stimulated various plant growth and biochemical attributes over untreated plants. At lower phosphate levels, substantial increase of 92, 65, and 200% in shoot length, fresh weight, and number of leaves was recorded with bacterial isolate HH2, whereas, at 30mM TCP, increase of 165% was observed in root length of plants treated with bacterial isolate KH3 compared to control. Similarly, at lower phosphate levels, increment of 57.3, 76.7, and 217% in phosphate, protein, and auxin content was recorded in plants treated with bacterial isolate HH2, and increase of 188.8% in total soluble carbohydrates was observed in plants treated with bacterial isolate KH3 as compared to control. Contrarily, increment in total chlorophyll content was most substantial (207%) by the bacterial isolate KH3 when provided with 30mM TCP. Hence, the current study reviled that the use of these phosphates (KH3 and HH2)-solubilizing PGPR, as an efficient phytostimulator used for crop production in the replacement of chemical fertilizers, is carcinogenic and deteriorating our eco-system.