Plant Weight Research Articles

Correlation and Path Analysis in Cowpea (Vigna unguiculata (L.) Walp.)

This study, conducted at Horticultural Research Farm of Babasaheb Bhimrao Ambedkar University, Lucknow, India, over 2020-21 and 2021-22, aimed to examine coefficient of correlation and direct and indirect effects of yield contributing traits on economic yield among 30 diverse cowpea genotypes. The research employed a Randomized Block Design (R.B.D.) with three replications, assessing twenty-six quantitative traits. Genotypic correlations exceeded phenotypic correlations, underscoring genetic influence over environmental factors. Key findings included plant height's positive correlation with branches per plant and days to first flowering, while negatively correlating with nodes on main branches and pod diameter. Traits such as number of pods per plant and average pod weight showed strong positive correlations with pod yield, highlighting their importance in breeding programs. Path coefficient analysis revealed significant positive direct effects on pod yield per plant from traits including plant height, branches per plant, and average pod weight. Indirect effects through traits like days to first flowering and non-reducing sugars also contributed to pod yield. These insights into trait correlations and effects are crucial for developing superior cowpea genotypes with enhanced yield and agronomic traits. The findings emphasize the importance of genetic variability in breeding programs, enabling the selection of superior genotypes to improve cowpea productivity.

Zinc Oxide Nanoparticles: An Influential Element in Alleviating Salt Stress in Quinoa (Chenopodium quinoa L. Cv Atlas)

Climate change has intensified abiotic stresses, notably salinity, detrimentally affecting crop yield. To counter these effects, nanomaterials have emerged as a promising tool to mitigate the adverse impacts on plant growth and development. Specifically, zinc oxide nanoparticles (ZnO-NPs) have demonstrated efficacy in facilitating a gradual release of zinc, thus enhancing its bioavailability to plants. With the goal of ensuring sustainable plant production, our aim was to examine how green-synthesized ZnO-NPs influence the seedling growth of quinoa (Chenopodium quinoa L. Cv Atlas) under conditions of salinity stress. To induce salt stress, solutions with three different NaCl concentrations (0, 100, and 200 mM) were prepared. Additionally, Zn and ZnO-NPs were administered at four different concentrations (0, 50, 100, and 200 ppm). In this study, plant height (cm), plant weight (g), plant diameter (mm), chlorophyll content (SPAD), K/Na value, Ca/Na value, antioxidant enzyme activities (SOD: EU g−1 leaf; CAT: EU g−1 leaf; POD: EU g−1 leaf), H2O2 (mmol kg−1), MDA (nmol g−1 DW), proline (µg g−1 FW), and sucrose (g L−1), content parameters were measured. XRD analysis confirmed the crystalline structure of ZnO nanoparticles with identified planes. Salinity stress significantly reduced plant metrics and altered ion ratios, while increasing oxidative stress indicators and osmolytes. Conversely, Zn and ZnO-NPs mitigated these effects, reducing oxidative damage and enhancing enzyme activities. This supports Zn’s role in limiting salinity uptake and improving physiological responses in quinoa seedlings, suggesting a promising strategy for enhancing crop resilience. Overall, this study underscores nanomaterials’ potential in sustainable agriculture and stress management.

Mitigating Combined Boron and Salt Stress in Lettuce (Lactuca Sativa L. Semental) through Salicylic Acid-Modified Rice Husk Biochar

Purpose: This study thoroughly investigates innovative amendment salicylic acid (SA) modified rice husk biochar (SABC) designed to improve boron (B) and salinity tolerance in lettuce, providing a comprehensive exploration of their potential effects in alleviating stress-induced challenges. Methods: Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and Raman spectroscopy were used for the molecular and chemical characterization of the biochar samples. The treatments consisted of control, 40 mM NaCl plus 20 mg B kg-1 (NaCl + B), and 40 mM NaCl plus 20 mg B kg-1 and 5 g kg-1 SA-modified rice husk biochar (NaCl + B + SABC). Results: Under conditions of salt and B toxicity, SABC treatment significantly prevented the decrease in plant weight induced by stress. SABC reduced the concentrations of B, sodium (Na), and chloride (Cl) in plants, while increasing the concentrations of potassium (K) and silicon (Si). The hydrogen peroxide concentration, which increased as a result of B and salt toxicity, was decreased with SABC. The activities of the antioxidant enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APX) showed a significant increase under stress, but due to the positive effect of SABC in reducing B and salt stress, there was a decrease in the activities of these enzymes. Conclusions: The results obtained from this study indicate that SABC is effective in reducing boron and salt stress. Testing the SABC molecule in different plants and under various stress conditions could provide significant contributions to the stress literature.

Biological control of soybean charcoal rot by native Trichoderma koningiopsis in Tucumán, Argentina

Macrophomina phaseolina (Tassi) Goid. (Mp) is the causal agent of charcoal rot in soybean, an economically serious and potentially destructive disease. Chemical control sometimes has limited effects on disease severity and may pose a threat to the environment, so the use of Trichoderma as a biological product could be a feasible alternative for charcoal rot management. The objective of this research was to isolate and characterize native Trichoderma spp. from northwestern Argentina and study their antagonistic effect against Mp in soybean. Isolations were performed from soil samples followed by cultural and microbiological characterization of the Trichoderma spp. isolates collected. Then, the antagonist effect against Mp was studied in vitro, under greenhouse conditions, and finally under field conditions with pathogen artificial inoculations during two soybean crop seasons (2020 and 2021). The results demonstrated that isolate Tr009 had a biocontrol effect against Mp in soybean. This control was evidenced in vitro and under controlled and field conditions with high emergency of plants compared to the Mp inoculated control. Under field conditions, treatments that included Trichoderma presented lower disease severity, colony-forming unit index and disease severity index values than the pathogen inoculated control. Moreover, Tr009 improved weight and length of soybean plants under greenhouse conditions. Isolate Tr009 was identified as Trichoderma koningiopsis by molecular methods. These results indicate that this biological tool can be used against Mp and thus favor a sustainable management of soybean charcoal rot.

Foliar-applied melatonin and titanium nanoparticles modulate cadmium (Cd) toxicity through minimizing Cd accumulation and optimizing physiological and biochemical properties in sage (Salvia officinalis L.).

Notwithstanding the fact that melatonin (MT) and titanium nanoparticles (Ti NPs) alone have been widely used recently to modulate cadmium (Cd) stress in plants, there is a gap in the comparative impacts of these materials on lowering Cd toxicity in sage plants. The objective of this study was to determine how foliar application of MT and Ti NPs affected the growth, Cd accumulation, photosynthesis, water content, lipid peroxidation, and essential oil (EO) quality and quantity of sage plants in Cd-contaminated soils. A factorial experiment was conducted using MT at 100 and 200μM and Ti NPs at 50 and 100mg L-1 topically, together with Cd toxicity at 10 and 20mg Cd kg-1 soil. The results showed that Cd toxicity decreased plant growth and enhanced lipid peroxidation. The Cd stress at 20mgkg-1 soil resulted in increases in Cd root (693%), Cd shoot (429%), electrolyte leakage (EL, 29%), malondialdehyde (MDA, 72%), shoot weight (31%), root weight (27%), chlorophyll (Chl) a + b (26%), relative water content (RWC, 23%), and EO yield (30%). The application of MT and Ti NPs controlled drought stress by reducing MDA and EL, enhancing plant weight, Chl, RWC, and EO production, and minimizing Cd accumulation in plant tissues. The predominant compounds in the EO were α-thujone, 1,8-cineole, β-thujone, camphor, and α-humulene. MT and Ti NPs caused α-thujone to rise, whereas Cd stress caused it to fall. Based on heat map analysis, MDA was the trait that was most sensitive to treatments. In summary, the research emphasizes the possibility of MT and Ti NPs, particularly MT at 200μM, to mitigate Cd toxicity in sage plants and enhance their biochemical reactions.

Optimization of keratin hydrolysis from sheep wool waste for enhanced crop performance: A sustainable approach in agriculture

Protein hydrolysates have emerged as potent enhancers of agricultural productivity, attributed to their nitrogen and amino acid richness. This study focuses on extracting keratin from sheep wool waste via water-based hydrolysis, aiming for eco-friendly alternatives to chemical methods. To refine this process, a novel response surface methodology integrating a Box-Behnken design (RSM-BBD) was devised, centering on temperature and hydrolysis time as pivotal factors affecting yield. Optimization yielded an impressive 99% w/v hydrolysis yield, with a fixed solid-liquid ratio (15:100 w/v) yielding 18.72 g/l of total nitrogen extraction. Analysis revealed a dominant presence of phenylalanine, noted for its role in plant water conservation. Agricultural trials demonstrated the hydrolysate's efficacy in enhancing maize crop physiology, evidenced by increased leaf surface area and fresh and dry plant weights across varied application rates. These results underscore the value of this innovative valorization process in agriculture. By harnessing keratin from sheep wool waste through water-based hydrolysis, the study proposes a sustainable alternative to traditional chemical techniques. The optimization of key parameters and subsequent positive impacts on maize crop physiology illustrate the potential of this approach to foster sustainable agricultural practices.

Impacts of Calcium Cyanamide Application as a Nitrogen Source on Growth, Yield, Quality, and Storage Durability of Short-day Onion

Rapid leaching of soluble nitrogen (N) sources in soil poses a significant challenge in agricultural practices. Therefore, gaining a comprehensive understanding of crop responses to slow-release N application rates has become crucial to contributing valuable insights to optimize N management strategies in agriculture. A field study was conducted to investigate the influence of preplant calcium cyanamide fertilizer on the growth, yield, quality, and shelf life of short-day onion. Six levels of calcium cyanamide (CaCN2, 19.8% N), 0, 90, 120, 200, 400, and 600 kg⋅ha−1 CaCN2, which are equivalent to 0, 17.82, 23.76, 39.6, 79.2, and 118.8 kg⋅ha−1 N, respectively, replicated four times were broadcasted and incorporated into the top 5 to 10 cm of soil. Using 400 kg⋅ha−1 of CaCN2 yielded noteworthy improvements in various parameters of onion growth, such as plant height, leaf count, bulb weight per plant, bulb diameter, bulb length, and overall plant weight, as indicated by the study results. The application of different levels of CaCN2 as an N source exerted a significant influence on these growth factors. Moreover, the study revealed a direct correlation between CaCN2 application levels and the storage life of onions. Specifically, the findings demonstrated that the application of 400 kg⋅ha−1 CaCN2 resulted in enhanced yield and overall onion plant growth. However, the application of 600 kg⋅ha−1 CaCN2 increased the incidences of bulb weight loss, rots, and sprouting during the 8-week storage period at room temperature. These findings provide valuable insights for onion investors and farmers in the region and offer practical recommendations for optimizing fertilizer use and storage practices to improve onion production and minimize postharvest losses.

Growth and yield response of maize to integrated nutrient management of chicken manure and inorganic fertilizer in different agroecological zones

The productivity of maize, an essential staple food crop in Africa, is severely constrained by the declining fertility of the soil. The combined use of organic and inorganic fertilizers could ameliorate this challenge in a sustainable way to boost maize productivity. Two field trials were conducted at Ashanti -Mampong and Damongo, in the transitional and Guinea Savannah agroecologies of Ghana respectively, to assess the influence of sole and integrated application of chicken manure and NPK fertilizer on the growth and yield of maize. The treatments included two maize varieties; Abontem and Obatanpa, and five fertilizer rates; 3 t/ha chicken manure (CM), NPK (65:38:38 kg ha−1 NPK), ½ CM + ½ NPK and ¾ CM + ¼ NPK and control (no fertilizer). A 2 x 5 factorial randomized complete block design with three replications was used. Significant variations (p < 0.05) were revealed between varieties, fertilizer types and their interaction effects for phenological, growth and yield of maize in both locations. The superiority of the integrated application of NPK and chicken manure was also visible in the vegetative parameters such as plant height, number of leaves and shoot dry weight of the maize plants given them the comparative advantage to assimilate more photosynthates for partitioning to the ears and grains. Obatanpa treated with ½ CM + ½ NPK produced the highest yield of 4661.1 kg ha−1, which was 29.6 and 29.9 % higher than the same variety grown on sole NPK and sole chicken manure, respectively at Damongo. Abontem treated with ¾ CM + ¼ NPK recorded a grain yield of 4479.3 kg ha−1, 11.7 % higher than the sole NPK and 10.3 % higher than the sole CM at Damongo. Similarly, Obatanpa grown on the ½ CM + ½ NPK recorded the highest grain yield of 4349.3 kg ha−1at Mampong followed by Abontem treated with the sole NPK (4267.1 kg ha−1). Sole NPK and the combined application of NPK and chicken manure gave comparable responses for vegetative traits while the integrated application proved superior for grain yield of maize across the two agroecologies.

Growth Characteristics and Quality of Cherry Tomatoes Planted with Nasturtiums, as a Companion Plant, in Building-Integrated Urban Agriculture

Background and objective: This study was conducted to promote a companion plant for building-integrated urban agriculture and determine appropriate spatial conditions for planting cherry tomatoes (Solanum lycopersicum) and nasturtiums (Tropaeolum majus), a companinon plant, in response to the increasing demands for eco-friendly agricultural practices in urban.Methods: The growth and quality of cherry tomatoes (CTs) and nasturtiums achieved with different planting ratios were analyzed: CC (when only CTs were planted), C2N1 (ratio of CTs to nasturtiums: 2:1), C1N1 (1:1), C1N2 (1:2), NC (when only nasturtiums were planted) on the rooftop and wall. A one-way ANOVA was used to evaluate the significance of test results based on planting ratios, followed by Duncan's multiple range test at a 95% confidence level. Independent sample t-tests were conducted to examine the growth and quality of CTs between the rooftop and wall.Results: Overall, CTs in CC showed significant growth compared to companion planting with nasturtiums. Among the treatment plots, the CTs in C2N1 had the highest plant weight and sugar concentrations on the rooftop. Similar patterns were observed on the wall. When planted in a 2:1 ratio as a companion plant to CTs (C2N1), nasturtiums had larger leaves and flowers. In terms of leaf size, plant weight, fruit weight, and sugar content CTs appeared to grow better on the wall than on the rooftop when planted with nasturtiums. For optimal CTs growth and quality, it is recommended to plant CTs in a 2:1 ratio with nasturtiums on the wall.Conclusion: Due to the distinctive spatial features of urban agriculture using buildings, specialized management strategies for different crops should be suggested. Further research should focus on a quantitative analysis of fruit and seed quality, as well as on a qualitative analysis to comprehensively understand the potential outcomes.

The Individual and Combined Effects of Cystoseira compressa Extracts and Inoculation of Arbuscular Mycorrhizal on Growth and Yield of Wheat under Salinity Conditions

Combined treatments are a successful way to overcome salinity damage in an environmentally safe and cost-effective method. So this experiment aimed to study the individual and combined effects of a seaweed extract of Cystoseira compressa (SWE) and Arbuscular Mycorrhizal Fungi (VA-M) on the growth and yield of Triticum aestivum L. cultivar (ACSAD 1398), under salinity conditions. In general, the study showed a significant decrease in morphological and biochemical parameters of the wheat under salinity levels. On the contrary, the results showed that all treatments significantly increased shoot and root length, number of leaves /plant, leaf area, seedling length, fresh and dry weight seedlings, spike length, fresh and dry weight spike, chlorophyll (a b), carotenoids, total pigments, Ca, Mg, P, K, Cu, N, crude protein, and total soluble sugars. As caused a decrease in proline content. The findings revealed that the (SWE+VA-M) combined treatment was superior to the foliar individual application of (SWE), and (VA-M) individual inoculation.

Growth response of tissue culture-derived bamboo (Bambusa tulda Roxb.) Plantlets to sources and levels of inorganic nitrogen

Information on nitrogen nutrition of bamboo plants during the nursery stage is very limited. The study was conducted to generate more information regarding the nitrogen nutrition of bamboo (Bambusa tulda Roxb.) specifically to evaluate the effect of forms and levels of N on the early growth stage performance of tissue culture-derived bamboo plantlets and identify the best form and optimum level of nitrogen for bamboo plants during the nursery stage. The acclimatized tissue culture-derived bamboo (Bambusa tulda Roxb.) plantlets were grown in black polyethylene bags measuring 24x15cm containing potting medium composed of 1:1v/v mixture of garden soil and rice hull charcoal. These were applied with two forms of nitrogen (N1-nitrate-N and N2-ammonium-N) which served as the factor A and 3 levels of nitrogen (L1-0g N plant-1, L2-0.25g N plant-1, and L3-0.50g N plant-1 which was the factor B of a 2×3 factorial RCBD experiment. The effects of the treatments on the performance of the bamboo plantlets were evaluated by gathering growth parameters such as height, number of leaves and culms and leaf size, and dry weights of leaf, stem, root, and total biomass weight. The form of N applied did not significantly influenced the size of bamboo plants but significantly affected the plant dry weight. Plants fertilized with ammonium-N produced heavier biomass weight than those applied with nitrate-N. The levels of N-application significantly influenced both the plant size and weight. Application of 0.25g N and 0.5g N plant-1 significantly increased the number of culms and leaves and total plant biomass dry weight. Based on the effect on the size and weight of plants, application of 0.25g N plant-1 was already optimum for bamboo plants during the nursery stage. For bamboo plants at the nursery stage, ammonium-N was better than nitrate-N with 0.25g N plant-1 as the optimum level of application.

Low-Cost Imaging to Quantify Germination Rate and Seedling Vigor across Lettuce Cultivars.

The survival and growth of young plants hinge on various factors, such as seed quality and environmental conditions. Assessing seedling potential/vigor for a robust crop yield is crucial but often resource-intensive. This study explores cost-effective imaging techniques for rapid evaluation of seedling vigor, offering a practical solution to a common problem in agricultural research. In the first phase, nine lettuce (Lactuca sativa) cultivars were sown in trays and monitored using chlorophyll fluorescence imaging thrice weekly for two weeks. The second phase involved integrating embedded computers equipped with cameras for phenotyping. These systems captured and analyzed images four times daily, covering the entire growth cycle from seeding to harvest for four specific cultivars. All resulting data were promptly uploaded to the cloud, allowing for remote access and providing real-time information on plant performance. Results consistently showed the 'Muir' cultivar to have a larger canopy size and better germination, though 'Sparx' and 'Crispino' surpassed it in final dry weight. A non-linear model accurately predicted lettuce plant weight using seedling canopy size in the first study. The second study improved prediction accuracy with a sigmoidal growth curve from multiple harvests (R2 = 0.88, RMSE = 0.27, p < 0.001). Utilizing embedded computers in controlled environments offers efficient plant monitoring, provided there is a uniform canopy structure and minimal plant overlap.

Differential responses of Meloidogyne spp. to Pasteuria isolates over crop cycles

In the present study examining the interaction between Meloidogyne spp. and Pasteuria isolates over three crop cycles, significant differences were observed in eggmass production, root galling, plant root weight, endospore production, and parasitism rates. Higher eggmass production was noted in the Meloidogyne blend treatment, whereas M. incognita showed a marked decline after the third cycle. Root galling varied significantly with nematode populations and Pasteuria isolates, showing notable interaction effects (P0.01). The Meloidogyne blend treatment exhibited the highest gall rating, while M. javanica and M. incognita treatments with Pp3 and PpEcu isolates resulted in lower galling. Fresh root weights of tomato plants differed significantly in the presence of Meloidogyne spp. and Pasteuria isolates, with significant interactions observed (P0.05). Greater root weights were recorded in M. javanica and M. incognita treatments with Pp3 and PpEcu isolates. The production of Pasteuria endospores in Meloidogyne females also showed significant variability across crop cycles (P0.01), with M. incognita and PpEcu treatments yielding the highest endospore numbers. Moreover, parasitism of Meloidogyne females by Pasteuria was significantly influenced by nematode population and isolate presence (P0.01). Higher numbers of infected M. incognita females were found in PpEcu treatments. Final female populations of Meloidogyne spp. varied significantly under Pasteuria influence, with the Meloidogyne blend treatment showing the highest population and M. incognita under PpEcu the lowest. The results highlight the complex interactions between nematodes and the bacteria, emphasizing the potential of Pasteuria isolates to influence various aspects of Meloidogyne spp. development.

Respon Tanaman Sawi Hijau (Brassica juncea L.) dan Status Hara Nitrogen Tanah Terhadap Pemberian Pupuk Kandang dan Phonska Pada Berbagai Dosis

This research aims to determine the response of green mustard plants (Brassica juncea L.) to the application of cattle manure and phonska at various rates, and to determine Nitrogen status of the soils. The experiment was set up using a Completely Randomized Design (CRD) which consisted of 2 factors. The first factor was the application of 15:15:15 phonska with three doses, namely ; P1 = 100 kg/ha, P2 = 200 kg/ha, and P3 = 300 kg/ha. The second factor was cattle manure in 4 doses, namely ; K1 = 5 tons/ha, K2 = 10 tons/ha, K3 = 15 tons/ha, and K4 = 20 tons/ha. So that obtain 12 treatments. Each treatment was repeated 3 times, resulting in 36 experimental polybags. The results showed that the interaction of cattle manure and Phonska had influence on plant parameters, namely plant height at 28 days after planting, fresh and dry weight, and fresh weight of root, while on soil parameters, namely pH and C-Organic. The application of Phonska had a significant effect on plant parameters, namely plant height at 14 and 28 days after planting, number of leaves at 14, 21 and 28 days after planting, leaf area, fresh and dry weight, and fresh weight of root, while soil parameters such as soil pH and C-Organic. And cattle manure had a significant on soil parameters, namely pH and total soil N.

Optimal Nitrogen Fertilizer Rates for Soybean Cultivation

The soybean (Glycine max. L. Merr) can satisfy a large portion of its requirement for nitrogen (N) by living in symbiosis with symbiotic bacteria. However, this source of N may be inadequate in varieties with high yield potential. To fully exploit this potential, soybeans should additionally utilize mineral forms of nitrogen present in the soil. The aim of this study was to determine the effect of varied nitrogen fertilizer application rates on the dry weight of the separated parts of soybean plants and the whole plant, including the number and weight of root nodules, the potential to reduce atmospheric nitrogen (N2), and the content and uptake of nitrogen. Four levels of pre-sowing nitrogen fertilizer supply were tested: 0, 60, 120, and 180 kg N·ha−1. Measurements of the tested parameters were taken during the flowering stage and the fully ripe stage. During the flowering stage, a reduction in the number of root nodules was observed following the application of 120 and 180 kg N·ha−1. In the fully ripe stage, each increase in nitrogen application caused a systematic decrease in the number of nodules on the roots. Increasing the level of nitrogen application therefore reduced the N2 fixation potential of soybeans, regardless of the developmental stage. The use of high doses of nitrogen in soybean cultivation did not increase seed yield or the weight of the entire plant. With high doses of nitrogen, the content and accumulation of nitrogen in soybean seeds and total mass did not increase. Therefore, the content and yield of crude protein did not increase. The main organ of nitrogen accumulation in the soybean flowering stage was the leaves (58.6–64.8% of total N uptake), however, in the fully ripe stage, it was the seeds (66.8–74.2% of total N uptake).

The growth-promoting and disease-suppressing mechanisms of Trichoderma inoculation on peanut seedlings.

Trichoderma spp. is known for its ability to enhance plant growth and suppress disease, but the mechanisms for its interaction with host plants and pathogens remain unclear. This study investigated the transcriptomics and metabolomics of peanut plants (Arachis hypogaea L.) inoculated with Trichoderma harzianum QT20045, in the absence and presence of the stem rot pathogen Sclerotium rolfsii JN3011. Under the condition without pathogen stress, the peanut seedlings inoculated with QT20045 showed improved root length and plant weight, increased indole acetic acid (IAA) production, and reduced ethylene level, with more active 1-aminocyclopropane-1-carboxylate acid (ACC) synthase (ACS) and ACC oxidase (ACO), compared with the non-inoculated control. Under the pathogen stress, the biocontrol efficacy of QT20045 against S. rolfsii was 78.51%, with a similar effect on plant growth, and IAA and ethylene metabolisms to the condition with no biotic stress. Transcriptomic analysis of peanut root revealed that Trichoderma inoculation upregulated the expression of certain genes in the IAA family but downregulated the genes in the ACO family (AhACO1 and AhACO) and ACS family (AhACS3 and AhACS1) consistently in the absence and presence of pathogens. During pathogen stress, QT20045 inoculation leads to the downregulation of the genes in the pectinesterase family to keep the host plant's cell wall stable, along with upregulation of the AhSUMM2 gene to activate plant defense responses. In vitro antagonistic test confirmed that QT20045 suppressed S. rolfsii growth through mechanisms of mycelial entanglement, papillary protrusions, and decomposition. Our findings highlight that Trichoderma inoculation is a promising tool for sustainable agriculture, offering multiple benefits from pathogen control to enhanced plant growth and soil health.

Evaluating Ecological Nano-Calcium from Eggshells: Effects on Calcium Nutrition and Oxidative Stress in Lettuce Under Saline and Boron Toxicity

AbstractNano-fertilizers with higher efficacy compared to conventional fertilizers can provide advantage for plant cultivation in both productive and problematic soils. Therefore, this study aimed to determine the effect of nano-calcium (nano-Ca) on lettuce plants grown in saline-boron toxic soil. Nano-calcium fertilizer was prepared from eggshells. Functional and structural properties of nano-Ca was determined by scanning electron microscopy (SEM), x-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR) before plant experiment. The treatments was; control, 40 mM NaCl and 20 mg kg−1 B (NaCl + B), and 40 mM NaCl and 20 mg kg−1 B with 4 mM Nano-Ca (NaCl + B + nano-Ca). The nano-Ca significantly increased the dry weight and calcium (Ca) concentration of lettuce plants under saline-B toxic conditions. Although there was a decrease in the concentrations of sodium (Na), chloride (Cl), and boron (B) with nano-Ca treatment, it was not statistically significant. Salinity and boron toxicity lead to increased lipid peroxidation. In the present study, the production of malondialdehyde (MDA) as a marker for lipid peroxidation, along with a significant decrease in hydrogen peroxide (H2O2) concentration, was observed with the application of nano-Ca. There was no significant alteration in superoxide dismutase activity (SOD) observed in lettuce grown under saline and boron toxic conditions. However, catalase activity (CAT) increased with nano-Ca application, while the activity of ascorbate peroxidase (APX) decreased. The study results suggest that nano-Ca serves a protective function for lettuce plants cultivated under saline and boron toxic conditions.

Isolation and characterization of native antagonistic rhizobacteria against Fusarium wilt of chilli to promote plant growth.

In the eastern coastal regions of Odisha, wilt caused by Fusarium oxysporum f. sp.capsici is an extremely damaging disease in chilli. This disease is very difficult to manage with chemical fungicides since it is soil-borne in nature. The natural rhizosphere soil of the chilli plant was used to isolate and test bacterial antagonists for their effectiveness and ability to promote plant growth. Out of the fifty-five isolates isolated from the rhizosphere of healthy chilli plants, five isolates, namely Iso 01, Iso 17, Iso 23, Iso 24, and Iso 32, showed their highly antagonistic activity against F. oxysporum f. sp. capsici under in vitro. In a dual culture, Iso 32 (73.3%) and Iso 24 (71.5%) caused the highest level of pathogen inhibition. In greenhouse trials, artificially inoculated chilli plants treated with Iso 32 (8.8%) and Iso 24 (10.2%) had decreased percent disease incidence (PDI), with percent disease reduction over control of 85.6% and 83.3%, respectively. Iso 32 and Iso 24 treated chilli seeds have shown higher seed vigor index of 973.7 and 948.8, respectively, as compared to untreated control 636.5. Furthermore, both the isolates significantly increased plant height as well as the fresh and dry weight of chilli plants under the rolled paper towel method. Morphological, biochemical, and molecular characterization identified Bacillus amyloliquefaciens (MH491049) as the key antagonist. This study demonstrates that rhizobacteria, specifically Iso 32 and Iso 24, can effectively protect chilli plants against Fusarium wilt while promoting overall plant development. These findings hold promise for sustainable and eco-friendly management of Fusarium wilt in chilli cultivation.

Exploring the potential of green synthesized cerium oxide nanoparticles in mitigating chromium toxicity in maize

ProblemChromium (Cr) contamination in agricultural soils poses a significant threat to maize productivity and food security, necessitating the development of innovative strategies to enhance Cr tolerance and mitigate its toxic effects on plant growth and development. ObjectiveThe aims of current study are the green synthesis of cerium oxide nanoparticles (CeO2 NPs) using Jasminum officinale L. plant extract and their application to alleviate chromium stress in maize plants. MethodsFourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and elemental mapping methods were used to characterize the synthesized CeO2 NPs. The cerium oxide NPs (0, 25, 75 and 100 mg/L) treatment levels applied through foliar and seed priming to check growth, physiological responses and metal uptake by maize plants and a fully random design was used to set up a factorial experiment. ResultsThe findings showed that foliar application of 100 mg/L CeO2 NPs resulted in an improvement in the plant height (43 %), shoot fresh plant (41 %) and root fresh weight (84 %), and total chlorophyll concentrations (67 %) in maize plants as compared to seed priming (as 25, 32, 40 and 43 % respectively). It also effectively enhanced antioxidant enzymes including super oxide dismutase (75 %) and peroxidase (79 %). In maize plants, by foliar application over the control, CeO2 NPs reduce electrolyte leakage (53 %), malondialdehyde contents (32 %), and chromium content in roots (70 %) under chromium stress. ConclusionTherefore, these results suggested that increase in the concentration of CeO2 NPs more efficiently 100 mg/L has high ability against chromium stress and act as a promising solution to reduce metal concentration, enhance maize growth and promote sustainable agriculture. SignificanceThis study’s implications for future research on CeO2 NPs include elucidating their mechanisms in plant stress tolerance, exploring interactive effects with heavy metals, and expanding their use in plant stress management. Furthermore, this finding develops a novel strategy for improving maize cultivation in Cr-contaminated soils, contributing to sustainable agriculture and food security.

Defoliation level and timing affect popcorn yield traits in the Midwestern United States

Popcorn [Zea mays (L.) var. Everta] is an important field crop in the Midwestern United States. However, hailstorms are a major threat to ensuring high yield because of their effects on leaf photosynthesis, pollination, and stalk lodging. A multi-state study spanning six site-years (2020–2023) was conducted in two locations (Ohio and Nebraska) to determine how defoliation intensity at different stages affects yield loss in popcorn and compare these losses with current values in the National Crop Insurance Service (NCIS) Corn Loss Instructions. At each location the study was conducted in a randomized complete block design with four replicates. The treatments consisted of the combination of four levels of leaf loss (25, 50, 75 and 100 % leaf blade area removal) imposed at five developmental stages: V8 (10-leaf), V13 (15-leaf), VT, R2, and R4. The evaluated traits were plant population, grain yield, stalk lodging, and 100-kernel weight. All evaluated traits were affected by defoliation, except plant population. The treatments 100 % defoliation at VT and R2 stages were the most damaging in terms of grain yield reduction, number of ears per plant and 100-kernel weight for both locations. In Ohio, the greatest stalk lodging was observed at 100 %-R2 and in Nebraska, at 75 %-VT. For both locations, the actual yield losses in the defoliated treatments were lower than values currently listed in the NCIS Popcorn Leaf Loss Chart. These results indicate leaf loss chart values should be updated to accurately reflect yield losses from popcorn defoliation events. This evaluation did not impose damage to stalks (e.g., bruising) or to plant whorls as may be expected in natural events. Future research should incorporate other forms of plant damage such as those described to provide a better emulation of hail damage.