Plant Substances Research Articles

Material Affective Engagements: Examples from Ancient Mesopotamia

This article applies approaches from current emotion research on material affective scaffolds—objects made and used to enhance, and more generally transform, affective states—to the emerging field of study focusing on emotions in ancient Near Eastern societies. Its main goal is to extend the framework of 4E cognition—with its central notion that human cognition is embodied, embedded, enactive, and extended—to the realm of affective states, emphasizing that through our bodily interactions with material objects we transform not just our cognitive processes but also our emotions, moods, and so forth. Thereby, the present study seeks to contribute to the exploration of the relationships between sensory experiences, emotions, moods, and the material world by investigating the affective meanings that material things acquire through people’s entanglements with them. The study focuses on one particular class of objects—Mesopotamian amulets from the first millennium BC, which served as bodily adornments but were also understood to have the power to evoke affective responses through their activation in ritual performances. Referring to scholarly compendia in Mesopotamian cuneiform texts, this study demonstrates that these objects were recommended by healing experts to influence different affective states, both in oneself and others. It examines the connection between affective states and specific material features of the amulet components (consisting of minerals, metals, and plant and animal substances). Finally, Mesopotamian views of affective states and their management are compared with those of contemporary cognitive-affective science. This comparison shows that although there are some analogies, there are also important differences that depend mainly on different understandings of the human mind and agency. byUlrike SteinertJohannes Gutenberg University Mainzusteiner@unimainz.deandGiovanna ColombettiUniversity of ExeterG.Colombetti@exeter.ac.uk

Establishment of quality standards for syrup from viburnum fruit meal

An adjusted dosage form has been developed in the form of a syrup using low-waste technology — from the meal of the fruits of Viburnum viburnum. Viburnum fruit meal contains higher fatty acids, triterpenoids, phytosterols, and carotenoids. Medicines of plant origin have a number of undeniable advantages compared to synthetic ones, since their composition ensures safety and better tolerability due to the variety of biologically active substances (BAS) of plants, they can have a complex effect on the body, and they can be used for a long time. However, the main issue is that, despite their high bioavailability and cost-effectiveness, herbal medicines are complex to design and require the development of a number of technological solutions. Equally important are the issues of establishing quality standards — standardization of the developed dosage form. The syrup was prepared from simple sugar syrup with the addition of dry extract from viburnum fruit meal. Purpose of work. Development and establishment of quality standards for the developed dosage form — syrup from viburnum fruit meal. Materials and methods. High performance liquid chromatography (HPLC) method. Let us use it to establish standards in quantitative terms for chlorogenic acid. Permanganatometry. This technique was used to determine the quantitative content of the amount of tannins in terms of gallic acid. Results. It was established that the content of chlorogenic acid in the developed syrup is 0.214 ± 0.006 %, the content of tannins in terms of gallic acid is 0.494 ± 0.014 %. Indicators acidity (pH) — 3–5, density — 1,360 to 1,420 g/cm3, microbiological purity — category 3 B. Conclusion. The result of the research was the establishment of the main indicators of syrup quality, namely the quantitative content of the main biologically active substances — chlorogenic acid and tannins in terms of gallic acid. In addition, such indicators as acidity (pH), density, and microbiological purity were determined.

Thymol as a Component of Chitosan Systems—Several New Applications in Medicine: A Comprehensive Review

Thymol, a plant-derived monoterpene phenol known for its broad biological activity, has often been incorporated into chitosan-based biomaterials to enhance therapeutic efficacy. Using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, we conducted a systematic literature review from 2018 to 2023, focusing on the biomedical implications of thymol-loaded chitosan systems. A review of databases, including PubMed, Scopus, and Web of Science was conducted using specific keywords and search criteria. Of the 90 articles, 12 were selected for the review. Thymol-loaded chitosan-based nanogels (TLCBS) showed improved antimicrobial properties, especially against multidrug-resistant bacterial antagonists. Innovations such as bipolymer nanocarriers and thymol impregnated with photosensitive chitosan micelles offer advanced bactericidal strategies and show potential for bone tissue regeneration and wound healing. The incorporation of thymol also improved drug delivery efficiency and biomechanical strength, especially when combined with poly(dimethylsiloxane) in chitosan–gelatin films. Thymol–chitosan combinations have also shown promising applications in oral delivery and periodontal treatment. This review highlights the synergy between thymol and chitosan in these products, which greatly enhances their therapeutic efficacy and highlights the novel use of essential oil components. It also highlights the novelty of the studies conducted, as well as their limitations and possible directions for the development of integrated substances of plant and animal origin in modern and advanced medical applications.

Effects of the synergistic treatments of arbuscular mycorrhizal fungi and trehalose on adaptability to salt stress in tomato seedlings.

AMF improve the plant adaptability to salt resistance by increasing mineral absorption and reducing the damage of saline soil. Trehalose plays an important role in plant response to salt damage by regulating osmotic pressure. Together, the use of AMF and trehalose in tomato seedlings proved efficient in regulating host substance synthesis, osmosis, and antioxidant enzymes. These synergistic effects significantly improved seedling adaptability to salt stress by enhancing cell osmotic protection and cell antioxidant capacity, ultimately reducing losses to crops grown on land where salinization has occurred.

Biochar reduces the cadmium content of Panax quinquefolium L. by improving rhizosphere microecology

Cadmium (Cd) accumulation in American ginseng (Panax quinquefolium L.) can negatively impact its yield and safety. Our previous study found that biochar could reduce cadmium content of P. quinquefolius, however, the mechanism was yet to be elucidated. In the present study, we tested four treatments in order to reveal the mechanism by which this phenomenon occurs: control, Cd, Cd + biochar and biochar. The results showed that the following responses were induced by the addition of biochar under Cd stress. Firstly, the soil physicochemical properties were improved, this is especially true for the soil pH value and soil organic matter content, which were increased by 20.42 % and 15.57 %, respectively. Secondly, the relative abundances of several beneficial microorganism phyla; such as Proteobacteria, Bacteroidota and Actinobacteria; were increased by 10.69 %, 20.11 % and 60.86 %, respectively. Thirdly, treatment with biochar reduced the Cd content by increasing cadmium-chelated metabolites within the soil (e.g., naringenin, caffeic acid, and valine) and increasing detoxification substances in plants (e.g., malic acid, flavonoids, and fumaric acid). Changes in these metabolites were significantly correlated with rhizosphere microecology. In summary, biochar treatment reduced the Cd content in seedlings by improving the soil properties, rhizosphere community, soil metabolites, and plant metabolites.

Encapsulation Methods and Releasing Mechanisms of Encapsulated Active Drug

Substances of plant origin are chemically unstable and easily oxidized by external conditions. However, they have to remain in their bioactive form when they are used in food, pharmaceutical, nutraceutical and cosmetic industries. Encapsulation method is an effective technique that should protect them preventing the degradation of these active substances and thus prevent them from losing their activities.
 In this review, the strategies for encapsulating plant substances and their preparation methods including emulsification, atomization, coaxial electrospray system, lyophilization, coacervation, in situ polymerization, melt extrusion, supercritical fluid technology, fluidized bed coating etc. were discussed. The release mechanisms of plant active substances were also presented.
 The choice of an appropriate encapsulation technique and wall material depends on the final use of the product and the processing conditions involved. The use of liposomes, ethosomes, phytosomes, emulsions, microspheres, microcapsules, solid lipid nanoparticles including formulations based on plant substances has enhanced their therapeutic effects. With the use of all these encapsulation systems, the formulation is delivered in a targeted manner, giving it an on-site effect, and the bioavailability of the formulation is also improved. With these new drug delivery systems, actives and extracts used in herbal formulations exhibit improved stability, sustained release from the formulation, protection against toxicity, and improved therapeutic efficacy.
 Keywords : nanoparticles, bioactive substance, formulation

Evaluation of the antimicrobial ac- tivity of the extracts of oregano and marsh cinquefoil against gram-positive and gram-negative bacteria

One of the most critical problems in animal husbandry and public health is antibiotic resistance, associated with the widespread use of antibiotic drugs. As a result, pathogenic microorganisms develop antibiotic resistance, which creates the need to either increase their dosage or replace them with other medications. Phytobiotics are a promising alternative to antibiotic drugs. Phytobiotics are active substances of medicinal plants that exhibit antimicrobial properties against pathogenic microorganisms. The primary sources of antimicrobial activity of phytobiotics are phenolic and polyphenolic compounds. The research aimed to study the antimicrobial activity of oregano (O) extracts and marsh cinquefoil (MC). Alcohol extracts (AE) were prepared from dry raw materials by water-alcohol extraction, and then alcohol-free extracts (AFE) were obtained using vacuum distillation. The content of polyphenolic compounds was studied in the obtained extracts, and the hole method against pathogens such as Staphylococcus aureus, Escherichia coli and Salmonella enterica evaluated their antimicrobial properties. The results showed that in O AFE, the content of polyphenolic compounds was 1.487 mg/ml, in O AE 1.166 mg/ml, in MC AFE 1.349 mg/ml, and MC AE 1.074 mg/ml. All studied extracts showed antimicrobial activity against Staphylococcus aureus. The most significant zones of suppression (1.86 cm) were formed around the wells with O AFE, which were significantly (P<0.01) larger than in the samples with O AE. About gram-negative cultures, zones of suppression were found only in alcoholic extracts, which is most likely a demonstration of the antibacterial activity of ethanol.

Influence of Forest Strips on Changes in Soil Properties in the Agrolandscape

Background: In Russia 5.2 million hectares of protective forest plantations were created on agricultural land. Forest strips optimize moisture turnover, heat and gas exchange of the territory and transform agrarian landscapes into more sustainable forest-agrarian ecosystems (agroforest landscapes). Studying the impact of shelterbelt forests on the transformation of soil properties in the context of climate change (organic carbon storage) is an important problem. The aim of our research was to study the effect of shelterbelt forests on soil indicators and to digitally map the contours of forest-meliorated soils on the test site using GIS-technology and field studies. Methods: In the present study, the methodology of soil studies in agroforested soils (1978) and the methodology of assessing the accumulation of substances in plants and soils in agroforested landscapes were used to study forest-meliorated soils. We proceeded from the position that the reclamation effect of forest belts occurs at a distance of 30 heights. The research was conducted from 2016 to 2022. Result: Research has established, that the soil profile under the forest belt has an organogenic horizon A0 (forest floor); the thickness of the humus horizon (A+B1) under the forest belt is 8-10 cm higher compared to other zones. Spatial distribution of humus content in the A+B1 horizon from the forest belt to the centers of inter-belt squares (zone 20H) is not characterized by great variability and clear patterns and on average makes 1.15-1.2%. The increase in calcium carbonate (CaCO3) content in the soil occurs in the direction from the forest belt to the 20H zone. The indicator of dense residue in terms of the content of readily soluble salts is not relevant in estimating the forest belt’s influence on transformation of soil properties. A preliminary digital map of the contours of forest-reclaimed soils has been compiled. The obtained materials will serve as a basis for modeling the spatial and temporal dynamics of soils under forest belts in the zonal section and improving soil fertility in the interstrip space.

GmABCG5, an ATP-binding cassette G transporter gene, is involved in the iron deficiency response in soybean.

Iron deficiency is a major nutritional problem causing iron deficiency chlorosis (IDC) and yield reduction in soybean, one of the most important crops. The ATP-binding cassette G subfamily plays a crucial role in substance transportation in plants. In this study, we cloned the GmABCG5 gene from soybean and verified its role in Fe homeostasis. Analysis showed that GmABCG5 belongs to the ABCG subfamily and is subcellularly localized at the cell membrane. From high to low, GmABCG5 expression was found in the stem, root, and leaf of young soybean seedlings, and the order of expression was flower, pod, seed stem, root, and leaf in mature soybean plants. The GUS assay and qRT-PCR results showed that the GmABCG5 expression was significantly induced by iron deficiency in the leaf. We obtained the GmABCG5 overexpressed and inhibitory expressed soybean hairyroot complexes. Overexpression of GmABCG5 promoted, and inhibition of GmABCG5 retarded the growth of soybean hairy roots, independent of nutrient iron conditions, confirming the growth-promotion function of GmABCG5. Iron deficiency has a negative effect on the growth of soybean complexes, which was more obvious in the GmABCG5 inhibition complexes. The chlorophyll content was increased in the GmABCG5 overexpression complexes and decreased in the GmABCG5 inhibition complexes. Iron deficiency treatment widened the gap in the chlorophyll contents. FCR activity was induced by iron deficiency and showed an extraordinary increase in the GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation. Antioxidant capacity was enhanced when GmABCG5 was overexpressed and reduced when GmABCG5 was inhibited under iron deficiency. These results showed that the response mechanism to iron deficiency is more actively mobilized in GmABCG5 overexpression seedlings. Our results indicated that GmABCG5 could improve the plant's tolerance to iron deficiency, suggesting that GmABCG5 might have the function of Fe mobilization, redistribution, and/or secretion of Fe substances in plants. The findings provide new insights into the ABCG subfamily genes in the regulation of iron homeostasis in plants.

Biological effects and mechanisms of dietary chalcones: latest research progress, future research strategies, and challenges.

Dietary plants are an indispensable part of the human diet, and the various natural active compounds they contain, especially polyphenols, polysaccharides, and amino acids, have always been a hot topic of research among nutritionists. As precursors to polyphenolic substances in dietary plants, chalcones are not only widely distributed but also possess a variety of biological activities due to their unique structure. However, there has not yet been a comprehensive article summarizing the biological activities and mechanisms of dietary chalcones. This review began by discussing the dietary sources and bioavailability of chalcones, providing a comprehensive description of their biological activities and mechanisms of action in antioxidation, anti-inflammation, anti-tumor, and resistance to pathogenic microbes. Additionally, based on the latest research findings, some future research strategies and challenges for dietary chalcones have been proposed, including computer-aided design and molecular docking, targeted biosynthesis and derivative design, interactions between the gut microbiota and chalcones, as well as clinical research. It is expected that this review will contribute to supplementing the scientific understanding of dietary chalcones and promoting their practical application and the development of new food products.

Constructing graphene oxide/Au nanoparticle cellulose membranes for SERS detection of mixed pesticide residues in edible chrysanthemum.

Edible chrysanthemum is widely cultivated and used as an important ingredient of medicine, tea and multifunctional food. During the planting of chrysanthemum, pesticides are extensively used for preventing plant diseases and insect pests. To ensure the food safety of edible chrysanthemum, rapid detection methods are urgently needed for on-site inspection. In this study, a graphene oxide/Au nanoparticle (GO/Au NP) cellulose substrate was prepared through layer-by-layer assembly of GO and Au NPs on a mixed cellulose ester membrane. Surface-enhanced Raman spectroscopy (SERS) detection of four types of organophosphorus and organosulfur pesticides was achieved by filtering the extracting solution through the substrate and analysing SERS spectra. Qualitative and semi-quantitative detection of fenthion, phoxim, isocarbophos and thiram was accomplished with the detection limits of 38.01, 8.13, 48.97 and 8.74 ng mL-1, respectively. A spiking experiment further demonstrated the feasibility of this method for rapid and on-site detection of mixed pesticides in chrysanthemum. This study provides a new approach for rapid detection of multiple hazardous substances in flowering and herbal plants.

Biologically active substances of plants of the Cupressaceae family of the genus Thuya and the genus Juniperus for phyto- and aromatherapy

Biologically active substances of plants of the Cupressaceae family, especially the genera Thuja and Juniperus, are used in various sectors of the national economy. The quality of essential oils is determined by a set of chemical compounds and their concentrations. In this case, the quality is determined by the ratio between the macro- and micro-components of essential oils. More than 40 biologically active compounds in essential oils have been determined by gas chromatography, which may also depend on the growing region of these plants.

Identification and prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi from the dog population in Wrocław, Poland

In recent years, there has been growing interest in exploring the potential health benefits of incorporating natural plant substances into the diets of farm animals. Basket willow (Salix viminalis), rich in flavonoids, tannins, and salicylates, has emerged as a candidate with analgesic, anti-inflammatory, and anthelmintic properties, making it appealing for integration into ruminant farming practices. This study aimed to analyze the effects of dietary supplementation with basket willow on the neuroanatomical properties of the small intestine in farmed fallow deer. Immunohistochemical methods and morphometric measurements were employed to assess changes in noradrenergic, galaninergic and nitrergic innervation patterns. Twenty-four fallow deer were divided randomly, with 12 individuals placed in both a control group and an experimental group, with the latter receiving willow supplementation. The results revealed significant alterations in the morphology and innervation patterns of the enteric nervous system (ENS) in the experimental group, including a decrease in ganglion size and nitric oxide synthase (NOS) immunoreactive nerve fibers, and an increase in galanin immunoreactive neurons. These findings suggest a potential modulation of gastrointestinal function by willow supplementation. However, caution is warranted due to the high salicylate content in basket willow, which may pose risks to gastrointestinal integrity. Overall, while basket willow supplementation shows promise in enhancing animal farming methods, further research is needed to elucidate its mechanisms and optimize its use in ruminant farming practices.

Modern possibilities of phytotherapy for male infertility from the standpoint of evidence-based medicine

Introduction: Male infertility is an topical medical and social problem of modern reproductive medicine. Its pharmacotherapy is often empirical in nature, and the most popular method remains the use of various herbal substances (phytotherapy), the effectiveness of which remains still understudied in the framework of evidence-based medicine.
 Materials and Methods: The results of research, thematic, systematic and Cochrane reviews and meta-analyses were searched in Medline/PubMed medical databases over the past 5 years using the search queries "plants male infertility", "plants sperm", "phytotherapy male infertility", "phytomedicinal therapeutics male infertility", "systematic review", "meta-analysis", and "review".
 Results: The vast majority of herbal substances offered for the treatment of male infertility demonstrate insufficient or contradictory evidence base for their clinical effectiveness, although some of them can be very useful pharmacotherapeutic options in the combined therapy of male infertility.
 Conclusion: Not all plant substances with a "reproductive effect" positioned in them actually have proven reproductive effects in studies in humans, therefore, the choice of phytotherapeutic agents in the treatment of idiopathic male infertility should be currently approached extremely carefully, especially in cases when we choose phytotherapy as an option for empirical monotherapy of male reproductive disorders.

Epidemiology and control of strawberry powdery mildew: a review

Strawberry powdery mildew, caused by Podosphaera aphanis, is an economically important disease for strawberry production. Typical symptoms are white mycelium on all aerial parts of affected plants, with young host tissues being the most susceptible. The pathogen overwinters on infected leaves, either as mycelium or chasmothecia, although the quantitative role of chasmothecia in epidemics are not fully understood. In spring, under favourable conditions, the fungus sporulates, disseminating conidia and causing polycyclic infections. The disease is mainly controlled using synthetic fungicides, but there is increasing interest in sustainable alternatives, including microbial biocontrol agents (e.g., Ampelomyces quisqualis, Bacillus spp., Trichoderma spp.) and substances of plant or animal origin (e.g., Equisetum arvense, orange oil, chitosan, whey). Physical methods, (e.g. UV-C, ozone) are also promising alternatives to fungicides. All of these strategies should be combined with appropriate agronomic practices (e.g., overhead irrigation, canopy management) to create unfavourable environments for the pathogen. However, agronomic practices have never been assessed for P. aphanis. Disease forecasting models and DSSs, though available, are underutilized due to their complexity and lack of validation across locations. This review presents the current state of knowledge on P. aphanis the available methods for control of strawberry powdery mildew, and highlights knowledge gaps relating to this host/pathogen relationship.

Comprehensive Study of Chalcone Derivatives

Chalcones are flavonoid molecules that exist naturally and are essentially plant substances. These are the chemical compounds that have demonstrated a variety of intriguing biological actions with therapeutic potential against a variety of ailments. Anthranilic acid and orthophenylene diamine combine to produce benzimidazole. Additionally, the benzimidazole acetylated product goes via Claisen-Schmidt condensation with aryl aldehyde to create the respective chalcones. Due to phenolic groups and the existence of α, β unsaturated carbonyl groups, naturally occurring chalcones are polyhydroxylated in the aryl ring and exhibit a variety of biological activities, including antioxidant, antibacterial, antiinflammatory, and anticancer properties. Being natural precursors, chalcones are intermediates that are crucial for the synthesis of flavones. This review article emphasizes on the informative aspects, methods of synthesis, biological activities and applications of Chalcones.

Development of industrial technology of plant substance of viburnum opulus fruit liquid extract

Nowadays, the development of domestic herbal medicines for the treatment of diseases of the cardiovascular system remains one of the current problems in the health care system of Ukraine. Some types of plant raw materials that can be successfully used in the complex therapy of cardiovascular diseases include viburnum fruits (Viburnum opulus L.). Pharmacological activity, the antimicrobial, antioxidant and cardioprotective properties of the extract developed on their basis from the fruits of the viburnum opulus fruit liquid extract (VOFLE) have studied by preclinical in vitro researches.
 The aim of the work was the development of industrial technology of the domestic plant substance Viburnum opulus fruit liquid extract (VOFLE) for the purpose of it further introduction into the tablet dosage form.
 Experimental samples of viburnum opulus fruits of domestic origin were used as object of research, harvested in the experimental fields of the Institute of Vegetable and Melon growing of the National Academy of Agrarian Sciences of Ukraine (Kharkiv region) to obtain the plant substance VOFLE. Physicochemical and pharmacotechnological methods of research have been used in our work.
 As a result of physical, chemical and pharmacotechnological researches, a domestic plant substance – Viburnum opulus fruit liquid extract (VOFLE) has been developed and standardized by extracting viburnum fruits (Viburni opuli fructus) by water-ethanol mixture 50% at the general ratio of raw material: extractant – 1:10, the analysis of each production stage of the technological process was carried out in order to establish the main technological parameters that are subjected to monitoring during the introduction of plant substances in industrial production and during the validation of the technological process.
 The technology of the domestic herbal substance viburnum opulus fruit liquid extract (VOFLE) obtaining was theoretically justified and experimentally developed with the aim of its further introduction into a tablet medicinal form of cardioprotective and membrane-stabilizing activity. A technological block diagram was developed with the definition of its main critical stages of the production process of the herbal substance, as well as a production equipment scheme, which was tested in industrial conditions of the LLC «Pharmaceutical Company "Zdorovya"», Kharkiv.

Ratiometric luminescent sensor based on BSA-coated gold/silver nanoclusters for the selective determination and spatiotemporal imaging of gallic acid in plants.

Anewfluorescence sensing strategyhas been developed. Four bimetallic nanoclusters, gold/silver, gold/copper, gold/molybdenum and gold/cobalt, were prepared using bovine serum albumin (BSA) as a reducing and stabilizing agent. The fluorescence properties of four nanoclusters were explored by solid-state UV and XPS. The gold/silver nanoclusters (BSA-Au/Ag NCs) with the best ratiometric fluorescence properties for gallic acid (GA) in plants were selected to realize the sensitive detection of GA. GA affected the conformation of BSA, thereby disrupting the luminescent environment of the nanoclusters, resulting in a pronounced fluorescence quenching at 566nm. The ratiometric fluorescence signal (I566/I453) was used for trace detection of GAin plants. It has a wide response range of 1.25-40.0μM and a low detection limit of 45.27nM. GA was detected at 19.49μM in the plant extract, and the spiked recoveries ranged from 96.09 to 104.6%. In addition, due to the non-toxic and biocompatible properties of BSA, BSA-Au/Ag NCs have also been validated for fluorescence imaging of plant tissues. It realized the comparison of GA content in different parts of plants and the difference of GA content in plants after abiotic stress. Therefore, the developed strategy offers potential application for the analytical study of active substances in plants.

FORMATION OF THE NUMBER OF PHYTOPATHOGENIC MICROMYCETE POPULATIONS IN AGROCENOSES OF OAT

The formation of the number of the phytopathogenic micromycete populations as a factor in biological pollution of oat agrocenoses is an important research field, the study of which will make it possible to create some sustainable agroecosystems. Therefore, the influence of the environmental factors (abiotic, biotic, anthropogenic, etc.) on the formation of micromycete populations on the vegetative organs of oat plants using different plant cultivation technologies was studied. This article presents the results of the ecological assessment of oat plant varieties based on the indicators of its their influence on the population number, the frequency of its occurrence, and the intensity of micromycete sporulation. Vegetative organs of oat plants of Parliamentsky and Tembre varieties were selected by BBCH scale for cereals in the phases: 5 tillers detectable (25), node 5 at least 2 cm above node 4 (35), еnd of heading: inflorescence fully emerged (39). It was determined that certain climatic conditions like an abiotic factor (namely an increase in air temperature), frequent droughts, or rare but abundant rains, significantly influenced the formation of micromycete populations in the oat leaf microbiome. Plant growing technologies, as an anthropogenic factor, influenced the spectrum of species and their frequency of the occurrence on the vegetative organs of oats of different kinds of the varieties. Using the organic technology of plant cultivation led to diversification of the spectrum of micromycete populations, but with a lower frequency of occurrence of species compared to the traditional technology of plant cultivation. Also, the varieties of oat plants, as a biotic factor, in terms of some physiological substances of plants, are able to restrain the spread of micromycete populations on the vegetative organs of plants or stimulate them. It was found out that using the traditional and organic technologies of plant cultivation on the vegetative organs of Tembre oat variety, the population density, the frequency of micromycete species occurrence, and the intensity of sporulation were significantly lower compared to the indexes of Parliamentsky oat variety plants. This points to the fact that the cultivation of oat varieties capable of resisting the formation of micromycete populations on an ecologically safe level will ensure a decrease in the level of biological pollution of agrocenoses and at the same time increase the biosafety of plant raw materials.

Identification of Ergastic Subtances of The Order Poales

Ergastic objects are one of the non-protoplasmic components besides vacuoles. Ergastic objects in protoplasts can be solid and liquid.The purpose of the study was to determine how the structure and type of ergastic substances in plants of order poales. This research method includes descriptive research that describes the type and structure of ergastic substances in plants of order poales, the parts observed are bulbs, grains, stems to fruits that contain ergasticsubstances.Based on the results of research on the order of poales found that the order of poales has a diverse type of solid ergastics, such as amylum (starch) found in teapot grass plants (Cyperus rotundus), rice (Oryza sativa) and corn (Zea mays) with various shapes and types, there are also types of ergastic substances in the form of ca-oxalate crystals found in pineapple plants (Ananas comusus) and types of sugar-shaped egastic substances in sugar cane plants (Saccharum officinarum Linn).