Spice Plant Research Articles

Piper chaba, an Indian spice plant extract, inhibits cell cycle G1/S phase transition and induces intrinsic apoptotic pathway in luminal breast cancer cells.

Piper chaba (Piperaceae) is a medicinal spice plant that possesses several pharmacological activities. In the present study, we for the first time studied the effect of P. chaba extract on breast cancer cells. P. chaba stem methanolic (PCSM) extract produced time and dose dependent cytotoxicity in luminal breast cancer cells (MCF-7 and T47D) with a minimal toxicity in breast normal cells (MCF-10A) at 10-100 µg/mL concentration. PCSM extract exerts 16.79 and 31.21 µg/mL IC50 for T47D and MCF-7 cells, respectively, in 48 h treatment. PCSM significantly arrests the T47D cells at the G0/G1 phase by reducing the CCND1 and CDK4 expression at mRNA and protein levels. PCSM extract treatment significantly altered nuclear morphology, mitochondria membrane potential, and production of reactive oxygen species in T47D cells at IC50 concentration. Extract treatment significantly altered the Bax/Bcl-2 ratio and altered caspase 8 and 3 mRNA/protein levels in T47D cells. Confocal microscopy showed an increase in late apoptosis in PCSM extract-treated breast cancer cells at IC50 . Further, an increased caspase 9 and caspase 3/7 enzymatic activity was observed in test cells compared with nontreated cells. In conclusion, P. chaba phytocompound possesses the potential to induce cell cycle arrest and induce apoptosis in luminal breast cancer cells.

Unveiling Neuroprotective Potential of Spice Plant-Derived Compounds against Alzheimer's Disease: Insights from Computational Studies.

Alzheimer's disease (AD) is a serious threat to the global health care system and is brought on by a series of factors that cause neuronal dysfunction and impairment in memory and cognitive decline. This study investigated the therapeutic potential of phytochemicals that belong to the ten regularly used spice plants, based on their binding affinity with AD-associated proteins. Comprehensive docking studies were performed using AutoDock Vina in PyRx followed by molecular dynamic (MD) simulations using AMBER 14. The docking study of the chosen molecules revealed the binding energies of their interactions with the target proteins, while MD simulations were carried out to verify the steadiness of bound complexes. Through the Lipinski filter and admetSAR analysis, the chosen compounds' pharmacokinetic characteristics and drug likeness were also examined. The pharmacophore mapping study was also done and analyzed for best selected molecules. Additionally, principal component analysis (PCA) was used to examine how the general motion of the protein changed. The results showed quercetin and myricetin to be potential inhibitors of AChE and alpha-amyrin and beta-chlorogenin to be potential inhibitors of BuChE, exhibiting best binding energies comparable to those of donepezil, used as a positive control. The multiple descriptors from the simulation study, root mean square deviation (RMSD), root mean square fluctuation (RMSF), hydrogen bond, radius of gyration (Rg), and solvent-accessible surface areas (SASA), confirm the stable nature of the protein-ligand complexes. Molecular mechanic Poisson-Boltzmann surface area (MM-PBSA) binding free energy calculations indicated the energetically favorable binding of the ligands to the protein. Finally, according to pharmacokinetic properties and drug likeness, characteristics showed that quercetin and myricetin for AChE and alpha-amyrin and beta-chlorogenin for BuChE were found to be the most effective agents for treating the AD.

Effect of NP and Blended NPSB Mineral Fertilizer on Black Cumin Yield in Central Highlands of Ethiopia

Black cumin (Nigella sativa L) is an important spice and medicinal plant cultivated in Ethiopia. However, the seed yield of the crop is low due to low soil fertility and poor soil fertility management. A field experiment was conducted to determine the effect of nitrogen and phosphorus (NP) and blended nitrogen, phosphorus, sulfur, and boron (NPSB) fertilizers on seed yield and yield components of black cumin. The experiment consists of three levels of N/P2O5 (20/15, 40/30, and 60/45 kg N/P2O5 ha–1 in the form of UREA and tri-super phosphate); and four levels of blended NPSB (0, 50, 100 and 150 kg ha–1) laid in RCBD design arranged in a factorial and replicated three time. The research was conducted during the 2017/18 primary season in the central highlands of Ethiopia. The interaction of NP and blended NPSB significantly influenced yield and yield components (P ≤0.01). The application of 40/30 kg N/P2O5 and 150 kg blended NPSB ha–1 resulted in the highest seed yield (1.85 t ha–1) and the highest net benefit of (78,137 ETB ha–1). Therefore, the application of 40/30 kg N/P2O5 and 150 kg NPSB ha–1 resulted in more than double the current national average yield and benefits black cumin-producing farmers in the central highlands of Ethiopia.

Alleviation of Seed Dormancy in Kala Zeera (Bunium Persicum) Through Chilling and Phytohormones

Kala zeera (Bunium persicum) is a high-value medicinal spice plant. The seeds and extracts are used for culinary, perfumery, flavouring food and medicinal purposes. The commercial cultivation of kala zeera is hampered due to poor seed germination and deep physiological dormancy. To break the dormancy of kala zeera, three phytohormones, gibberellic acid (100 μmol L-1), 6-benzylaminopurine (10 μmol L-1) and thidiazuron (6.3 μmol L-1) were tested individually and in combination, under two temperature levels: continuous chilling at 4°C and one week chilling at 4°C followed by incubation at room temperature. The combined application of the three phytohormones under continuous chilling (4°C) improved the germination from 11.25 to 93.25% and reduced the mean germination time 60 to 50 days. The lowest mean germination time (34.78 days) and high mean daily germination (1.57 seeds day-1) with a high coefficient of germination velocity (2.88), were found under combined application of phytohormones as compared to their individual application. The findings have implications in successful propagation and systematic cultivation of this high-value medicinal spice plant.

Usage of spicy aromatic plants of the flora of Armenia in the national cuisine

Since ancient times, Armenians have used wild plants in their diet in both fresh and processed forms. Their gastronomic traditions, knowledge about useful plants, and methods of their application have been passed from generation to generation. In this review we analyse the species composition of plants that are used as spices in the national cuisine, how many there are, and what prospects there are for their use. Field surveys were conducted to collect ethnobotanical data of the use of aromatic plants in all of regions in Armenia during the years 2016–2022. 58 informants (42 women and 16 men) were interviewed after obtaining their prior informed oral consent. The informants included farmers, housewives and cooks engaged in preparing the national cuisine. All the informants participated voluntarily and shared their life experience, knowledge received from grandmothers and their professional skills. For the first time, a list of spicy plants of the flora of Armenia which were used in the preparation of national dishes is presented. Some species are no longer used, because classic spices are already available for sale, but some species of plants are still used today as a seasoning in national dishes. In the flora of Armenia there are 142 species of spicy aromatic plants belonging to 115 genera and 46 families. Studies have shown that the largest number of spicy-aromatic plants grow in Central and Southern Armenia (Armeno-Iranian floral province). On the basis of this rich plant resource, it is possible to organize the cultivation of organic spicy plants and spices in order to use them in various branches of the food industry. The recollected knowledge of our ancestors in the area of cheesemaking, winemaking, beermaking, combining centuries of experience with today's new technologies and use of spice plants can be used to develop the country's economy.

Agronomic assessment of three spice plants as understorey crops in oil palm (Elaeis guineensis Jacq.) plantation

Utilization of land with intercrop plants can support the productivity of the land. The wide spacing of oil palm plants can be used to plant intercrops. Spice plants such as red ginger, turmeric, and cardamom are examples of intercropping plants under the shade of oil palm. This study aimed to determine the adaptability of red ginger, turmeric, and cardamom as understorey plants of different oil palm ages. The research was carried out from February to November 2021 at Sekernan, Muaro Jambi, Jambi. The study consisted of three experiments to investigate three spice plants. Each experiment used a randomized complete block design (RCBD) with oil palm ages as treatments, namely 0, 5, 10, 15, and 20 years. Variables observed included light intensity, plant height, tiller number, leaf number, and rhizome weight. The red ginger and turmeric were considered as adaptive plants to understorey conditions due to the yield of > 60% in 15-year-old oil palm trees than those grown in non-shade conditions. Production levels of red ginger and turmeric were determined by the number of tillers and leaves. The cardamom plant under oil palm shade produced a better yield than that in control. This implies that all three spices are suitable as intercrop plants under oil palm plantations.
 Keywords: cardamom; low light intensity; red ginger; shade stands; turmeric

Ethnobotany of robusta coffee (coffea canephora pierre ex a. froehner) as a spiced coffee drink of the indigenous people of bale village on halmahera island

A study on inherited local knowledge of spiced coffee ethnobotany in processing coffee by the people of Bale Village is necessary to depict the sub-system coffee processing. Bale Village is situated on Halmahera Island. It is one of the villages in the Oba Sub-district, Tidore Kepulauan Regency. The village is known as a producer of coffee bean and coffee powder. This study on local knowledge is an initiative to acquire information on spiced coffee. The research design is a survey design to investigate and document the knowledge of the utilization of spice plants in coffee drinks as a traditional beverage of people in Bale Village. Data are analyzed in a qualitative-thematic manner to describe respondents’ ethnobotanical knowledge and are independent of the researcher’s assessment. The research results indicate that spiced coffee made by the villagers uses six spice plants, namely clove (Syzygium aromaticum (L.), red ginger (Zingiber officinale Roscoe.), cinnamon (Cinnamomum verum J. Presl.), kara benguk (Mucuna pruriens (L.) DC.), nutmeg (Myristica fragrans Houtt.), and pandan (Pandanus amaryllifoliusRoxb.). Stages in the making of spiced coffee with the 5M method include: 1) determine the composition of spiced coffee, 2) grind spiced coffee beans into powder, 3) prepare water, 4) stir the spiced coffee and 5) enjoy the spiced coffee

The THE EFFECT OF DIFFERENT GROWING MEDIA ON THE GROWTH AND PRODUCTION OF RED GINGER (Zingiber officinale Rosc)

· Introduction: Red Ginger (Zingiber officinale Rosc) is a spice plant that is used as a drink and a mixture of food ingredients. The spicy taste of ginger when drunk gives a sensation as a reliever. Ginger rhizome is also efficacious as a medicine for several diseases such as: arthritis, rheumatism, sprains, muscle aches, coughs, sinusitis, sore throat, diarrhea, colic, cramps, indigestion, loss of appetite, motion sickness, fever, flu, elimination, and infectious diseases.
 
 Materials and Methods: This research was carried out in Selayang Village, Finish District, Langkat Regency, North Sumatra, from September 2022 to completion in February 2023.
 Results: The results showed that the M1 treatment had significant results from almost all observation parameters, namely plant height, number of leaves and fresh weight of rhizomes had a significant effect, except for the parameter of the number of tillers. showed that the highest vegetative growth of red ginger was in treatment M1 (top soil and cow manure) resulting in the highest plant height compared to other treatments.
 
 Keywords: Red Ginger, Manure for Cows, Goats, Chickens, NPK.

Profil Kandungan Total Fenol, Flavonoid serta Antioksidan pada Buah Golden Berry (Physalis peruviana) Kamojang Jawa Barat

The adequate nutrition helps reduce the rate of immune suppression in HIV-infected individuals and promotes adherence to treatment. In contrast, inadequate nutrition and food insecurity tend to accelerate disease progression and are associated with poorer treatment outcomes for PLHIV. These conditions have occurred, associated with lower CD4 counts and decreased immunity, increased risk of transmission and metabolic syndrome, increased risk of infection, increased risk of death, poor adherence to HAART and reduced quality of life. Golden berry is scientifically known as Physalis peruviana. This fruit is the size of a marble and is yellowish in color in Indonesia, this fruit is known as Ciplukan. This study used an extract trial using complete randomized design. The test was carried out at the Spice and Medicinal Plants Laboratory, Bogor, West Java. The results showed that the Kamojang Golden Berries (GBK) grown at Daniel's Farm, Mount Kamojang, West Java, were proven to contain an antioxidant content of 376,17 ppm and antioxidant compounds: Flavonoids of 1.30 mg/100 g; Carotenoids of 8.48 ppm, means that GBK fruit has antiviral, anti-inflammatory and anticancer effects; and Phenol of 44.46 mg GAE/100 g, meaning that GBK fruit acts as an anti-aging and free radical scavenger. GBK fruit extract can be used as an alternative therapy in overcoming the problem of decreased immune system, especially CD4 count which often occurs in HIV/AIDS patients.

Elucidating the effects of cumin (Cuminum cyminum) fruit and stem as feed additives on growth, antioxidant capacity, liver and intestinal health, and gut microbiome of Nile tilapia (Oreochromis niloticus)

Cuminum cyminum L., commonly known as cumin, which belongs to the family Apiaceae and the order Apiales, is a widely grown spice and medicinal plant in Xinjiang province, China. The fruit of cumin is primarily edible and is also consumed for its medicinal properties. However, after the cumin fruits harvest, a large amount of plant stem waste is often produced, resulting in resource waste. In this study, fruits and stems of cumin were used at 1% and 3% concentrations (F1 and F3; S1 and S3, respectively) as feed additives to investigate the effects of their dietary supplementation on the growth, antioxidant capacity, liver, and intestinal health, and gut microbiota of Oreochromis niloticus. A total of 375 fish were fed, and their mean initial weight was 9.22 ± 0.17 g. After 8 weeks of feeding, the F1, S1, and S3 treatments significantly increased the weight gain rate and specific growth rate of O. niloticus. At the same time, S1 and S3 increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and decreased the malondialdehyde (MDA) levels. S1 and S3 also reduced the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum, reducing lipid droplet formation. Moreover, S1 and S3 significantly increased the length of intestinal villus and the abundance of Proteobacteria, reduced the abundance of Fusobacteriota and Bacteroidota in the gut, and increased the expression levels of metabolic pathways of the gut microbiome. The cumin stems and the fruits, at the lower 1% concentration, improved the growth performance of O. niloticus, accelerated growth rate, enhanced antioxidant capacity, improved intestinal morphology, and alleviated fatty liver disease. In particular, the stems of cumin have the potential to be used as feed additives for O. niloticus, providing a novel solution for the full utilization of agricultural waste.

Pengolahan Jahe Merah (Zingiber Officinale Var Rubrum Rhizoma) Menjadi Serbuk Jahe Merah (SJM) Sebagai Produk Potensial Desa Kelindang Atas

Red ginger (Zingiber Officinale Var Rubrum Rhizoma ) is a spice plant that has high potential to be developed as a value-added product. In the village of Kelindang Atas, red ginger plants are a source of income for the local community, but this potential has not been maximized due to a lack of knowledge and skills in red ginger processing. In Student Community Service (PMM) by the Faculty of Agriculture team, socialization was given to villagers regarding processing, packaging, and calculation of HPP from red ginger powder (SJM) as a red ginger processed product to increase the selling value of red ginger as a potential product from Kelindang Atas Village. Community service in the processing of red ginger in Kelindang Atas Village provides benefits in increasing the knowledge, skills and income of the local community through the sale of red ginger powder products produced

Chemical markers of a rare honey from the traditional spice plant Amomum tsao–ko Crevost et Lemarié, via integrated GC–MS and LC-MS approaches

The precious medicinal plant, Amomum tsao-ko Crevost et Lemarié, is the nectariferous plant from which the rare Amomum tsao-ko Crevost et Lemarié honey (ATH) is produced. Presently, chemical markers for authentication of this honey are not available due to the lack of data on its chemical composition. Here, we analyzed the volatile components and their odor activity values (OAVs), which revealed that the unique aroma was mildly flowery and fruity, accompanied by subtle sweet and fresh undertones. Since non-volatile chemicals are more reliable markers for routine authentication, we used a metabolomic approach combined with NMR-based identification to find and confirm a suitable compound to unambiguously distinguish ATH from other honeys. Isorhamnetin 3-O-neohesperidoside ranged from 3.62 to 9.38 mg/kg in ATH and was absent in the other tested honeys. In sum, the study uncovered unique chemical characteristics of ATH that will be helpful to control its quality.

Analisis gaya dan daya pada alat pengaduk mesin kristalisasi jahe dengan kapasitas 5 kg/jam

Red ginger is a plant that is easy to grow in Indonesia and is well known as a spice plant and can be found in many plantation farmers, such as in Kediri Regency, Kepung District, Besowo Village. One of the preparations of red ginger that local residents are involved in is making instant herbal medicine. The processing of instant herbal medicine with red ginger as raw material includes the process of washing, grating, squeezing, and cooking so that the final result is crystallized red ginger. The focus of discussion in this article is the mixing process. The design of this mixing machine uses a power of 0.5 HP and a rotation of 1400 rpm to mix 5 kg/hour of red ginger dough. This stirring process takes 60 to 80 minutes until red ginger crystals form.

EVALUASI KESESUAIAN LAHAN UNTUK PENGEMBANGAN TANAMAN REMPAH UTAMA DI DESA CUKILAN, KECAMATAN SURUH, KABUPATEN SEMARANG

The availability of information regarding the cultivation of spice plants is still limited, so studies are needed to support the development of spice plants. This study aimed to identify land characteristics, land suitability classes and distribution maps, limiting factors and recommendations for the development of spice cultivation. The analytical method used was Location Quotient (LQ), and Shift Share Analysis (SSA) to determine the main spice commodities. Overlay and matching between land characteristic parameters and land suitability criteria through ArcGis software version 10.4. The results of the study obtained two main commodities, namely cardamom, with an LQ of 1.98 and ginger, with an LQ of 1.05. Land suitability classes for cardamon are quite suitable (S2), covering an area of 583.89 ha (94.1%) and marginally suitable (S3), covering an area of 36.87 ha (5.9%) with drainage limiting factors, rainfall, base saturation, slope, CEC, pH, and organic C. Land suitability classes for ginger are moderately suitable (S2) with a land area of 541.71 ha (87.3%) and marginally suitable marginal (S3) covering an area of 79.06 ha (12.7%) with the limiting factors of rainfall, temperature, drainage, slope, base saturation, salinity, CEC, pH, and organic C.

The Development of Germination Tests and Breaking Dormancy Techniques of Bitter Seeds (Andrographis paniculata (Burm. f.) Wall. ex Nees)

Bitter seed (Andrographis paniculata (Burm. f.) Wall. ex Nees) is a medicinal species used in phyto pharmacy and known as ‘the king of bitterness’. Bitter seeds contain andrographolide,
 a diterpenoid lactone which is widely used in Indian medicine for snake bites and for the treatment of hepatitis. The research was conducted at the Seed Management Unit experimental field of the Indonesian Spice and Medicinal Plants Research Institute (IMACRI) and at the Seed Quality Testing Laboratory of IPB University, Indonesia, from January until May 2021. The objectives of the study is to determine the best germination test and effective breaking dormancy techniques to increase the germination percentage of bitter seeds. The study was arranged in a completely randomized design with two-factors. The first factor was seed treatments, i.e., control/untreated, chemical scarification with 5% H2SO4 for 10 minutes, and 5% H2SO4 followed by a 100 ppm GA3 soaking treatment. The second factor conducted was germination test methods according to International Seed Testing Association, i.e., germinating the seeds on the top of paper and in pleated paper. Our study demonstrated that seed treatment with H2SO4 can break seed dormancy and speed up seed germination. Bitter seed germination on the pleated paper was higher than with the top-of-paper method. Bitter seed germination with the top-of-paper method was at 65 DAS and the final germination was at 80 days after sowing (DAS), and the final germination was 55.2%. With the pleated paper method, the seeds germinated at 30 DAS and the final germination was at 70 DAS, and the final the germination of 59.2%.

Phytochemicals and Proximate Analysis of Litsea Cubeba Lour. Barks

Indonesia is a country with a lot of spice plant resources. Attarasa (Litsea cubeba L.), which is frequently found in the North Tapanuli area, is one form of spice that can be developed. Attarasa (Litsea cubeba L.) is a Lauraceae plant with varous biological activity such as antidepressant, anti-inflammatory, antioxidant, pesticide, antibacterial, anticancer, and neuropharmacology. An estimation of the nutrient composition, phytochemicals and proximate values of plants gives the idea of their nutritional significance. This study aimed to determine phytochemicals and proximate analysis in Litsea cubeba Lour. barks simplex powder. Analysis of phytochemicals were analyzed with thin layer chromatography (TLC) method and proximate (water and ash content, carbohydrate, protein, crude fiber and total fat) were analyzed based on SNI-01-2891-1992. Alkaloids, steroids/triterpenoids, tannins, saponins, flavonoids, glycosides were found in simplex powder. The water content, ash content, carbohydrate, protein, crude fiber and total fat of simple powder were 8.70%; 1.79%; 41.50%; 8.43%; 35.80% and 0.78% respectively. Simplex powder of Litsea cubeba Lour. barks has contained various of phytochemicals and proximate value.

A novel perspective on the preventive treatment of hydrazine compound-induced liver injury: Isoniazid liver injury as an example

Ethnopharmacological relevanceAnethum graveolens L. (dill), which has been used as a medicine, spice and aromatic plant since ancient times, is not only a traditional Chinese medicines but also an important medicinal and functional food in Europe and Central and South Asia. In ethnomedicine, dill reportedly exerts a protective effect on the liver and has been widely used as a traditional medicine for the treatment of jaundice in the liver and spleen and inflammatory gout diseases in Saudi Arabia. Furthermore, studies have found that dill can regulate the NAT2 enzyme, and this plant was thus selected to study its alleviating effect on isoniazid liver injury. Aim of the studyThe purpose of this study was to explore the effect of dill on alleviating liver injury induced by hydrazine compounds represented by isoniazid through the use of network pharmacology combined with in vivo and in vitro experimental verifications. Materials and methodsFirst, we screened the key targets of dill in the treatment of liver injury through the use of network pharmacology; we then performed GO and KEGG pathway enrichment analyses using the DAVID database. We also verified the alleviative and anti-inflammatory effects of dill on isoniazid liver injury in rats by animal experiments. We further investigated the modulating effect of dill on the enzymatic activity of NAT2, a common metabolizing enzyme of hydrazine compounds. ResultsA total of 111 key targets were screened through network pharmacology. In vivo experiments showed that dill reduced the amount of inflammatory factors produced by isoniazid, such as IL-10, IL-1β, TNF-α and IL-6, restored the levels of ALT, AST, r-GT, AKP and TBA in vivo, and attenuated isoniazid liver injury. Both in vivo and vitro results indicated that dill could regulate the expression of NAT2 enzymes. ConclusionsThe results tentatively demonstrate that dill can alleviate isoniazid liver injury through multiple components, targets and pathways and exerts a regulatory effect on the NAT2 enzyme, and these findings thus provide new ideas for subsequent studies on hydrazide liver injury——reducing the risk of hydrazide-induced liver injury through anti-inflammation and regulation of NAT2 enzymes.

Alteration of The Content of Piperine and Essential Oil from Black Pepper and White Pepper After a Year Storage

Pepper is a type of spice plant in Indonesia that used for cooking spices. One of the advantages of this spice is that it has a spicy taste and distinctive scent caused by piperine and essential oils contained in pepper seeds. This research was carried out from June 2018 to October 2019, using the main raw material for the Natar 1 variety of pepper. The pepper seed was processed into black pepper and white pepper, and the yield was calculated. Then observed levels of piperine and essential oils of black pepper and white pepper prior to and a year after storage. The data obtained were processed in a qualitative descriptive manner. The results showed that after 12 months of storage, there was a decrease in piperine content of 9.91% and a decrease in essential oil content of 21.77%; while white pepper decreased piperine content by 19.45% and essential oil content decreased by 56.91%. Based on the results of this study, good storage technology is needed to support the sustainability of the availability of black pepper and white pepper in particular, as well as pepper farming in general.

Dried nutmeg (Myristica fragrans Houtt) derivatives products processing in Tapaktuan District, South Aceh Regency

Nutmeg is one of the spice plants that can be found in the province of Aceh, nutmeg is processed into snacks managed by cooperatives small and medium enterprises. Due to a large number of nutmeg, processing into snacks is one of the regional economic empowerment. In this study, an analysis of the supply chain distribution of candied nutmeg and the level of consumer preference for processed nutmeg fruit into snacks was carried out. Processing of snacks in the form of dry nutmeg. The sample used is 14 units of cooperatives small and medium enterprises that are active in production in South Aceh. Acceptance and value of selling prices from upstream to downstream so that it affects the results of production carried out by producers to consumers. The sample generated from 14 small business units was tested on the level of consumer preference by using 25 panellists who were grouped into teenagers, adults and old consumers. The results of this study indicate that one small and medium-sized business has the highest score (UD Cahaya) in achieving the level of consumer preference. The selling price of these snacks at the production site is Rp. 4.000,- and the selling price reaches Rp. 5.000,- per pack.

Inventory of potential spice plants as care cosmetics

This research is motivated by the absence of previous research that examines the use of spice plants as care cosmetics by the people of Belonsat Village. This result can make vanished of knowledge about the efficacy of spice plants as care cosmetics, so it is necessary to have a instrument used to document in writing about spice plants used as care cosmetics by the people of Belonsat Village. This study aims to 1) identify the types of spice plants that are used as care cosmetics, 2) identify parts of spice plants that are used as care cosmetics, 3) identify how to use them as care cosmetics. The research uses a qualitative descriptive approach, the research method used is a survey. The source of the data from this research was obtained from the main informant who also served as a key informant. Data obtained through in-depth interviews and participatory observation. The results of an interview with a key informant as well as a key informant showed that there were 11 species of spice plants that have the potential as care cosmetics. These spice plants include: basil, cardamom, kencur, temu giring, ginger emprit (ginger rice), elephant ginger, red ginger, temulawak, lime, orange chili sauce, and garlic. There are uses that are singular, there are also those that must be combined.