Plant Size Research Articles

Allometric relationships and trade-offs in 11 common Mediterranean-climate grasses.

Biomass allocation in plants is the foundation for understanding dynamics in ecosystem carbon balance, species competition, and plant-environment interactions. However, existing work on plant allometry has mainly focused on trees, with fewer studies having developed allometric equations for grasses. Grasses with different life histories can vary in their carbon investment by prioritizing the growth of specific organs to survive, outcompete co-occurring plants, and ensure population persistence. Further, because grasses are important fuels for wildfire, the lack of grass allocation data adds uncertainty to process-based models that relate plant physiology to wildfire dynamics. To fill this gap, we conducted a greenhouse experiment with 11 common California grasses varying in photosynthetic pathway and growth form. We measured plant sizes and harvested above- and belowground biomass throughout the life cycle of annual species, while for the establishment stage of perennial grasses to quantify allometric relationships for leaf, stem, and root biomass, as well as plant height and canopy area. We used basal diameter as a reference measure of plant size. Overall, basal diameter is the best predictor for leaf and stem biomass, height, and canopy area. Including height as another predictor can improve model accuracy in predicting leaf and stem biomass and canopy area. Fine root biomass is a function of leaf biomass alone. Species vary in their allometric relationships, with most variation occurring for plant height, canopy area, and stem biomass. We further explored potential trade-offs in biomass allocation across species between leaf and fine root, leaf and stem, and allocation to reproduction. Consistent with our expectation, we found that fast-growing plants allocated a greater fraction to reproduction. Additionally, plant height and specific leaf area negatively influenced the leaf-to-stem ratio. However, contrary to our hypothesis, there were no differences in root-to-leaf ratio between perennial and annual or C4 and C3 plants. Our study provides species-specific and functional-type-specific allometry equations for both above- and belowground organs of 11 common California grass species, enabling nondestructive biomass assessment in California grasslands. These allometric relationships and trade-offs in carbon allocation across species can improve ecosystem model predictions of grassland species interactions and environmental responses through differences in morphology.

Local adaptation of a generalist hemiparasitic plant to one of its potential host plants

Coevolution is often found in parasite–host interactions, but has not yet been described for hemiparasitic plants and their hosts. Root hemiparasites like Rhinanthus alectorolophus perform photosynthesis but also parasitize other plant species, some of which (e.g. Plantago lanceolata) may defend themselves against parasite attack by blocking the haustoria of the parasites. We grew seedlings of the hemiparasite R. alectorolophus and the potential host P. lanceolata from seven grassland sites in a factorial design. To detect differences in host defence, we also included hosts from two ‘naïve' populations from regions where the parasite does not occur. R. alectorolophus grew consistently larger and had higher fitness with sympatric than with allopatric hosts, suggesting parasite adaptation to local host populations. Moreover, R. alectorolophus remained smallest with allopatric hosts from the same region and reached intermediate sizes with allopatric hosts from other regions or naïve hosts, suggesting host adaptation to parasites at the regional scale. Parasite presence reduced the size of the host plants already after four weeks, but only that of hosts with ‘experience' of the parasite, suggesting an early host response. Follow‐up experiments confirmed that parasites attach to hosts already after four weeks and hosts respond by changing belowground allocation patterns. However, parasite roots did not preferentially grow towards sympatric hosts. Our results suggest that local adaptation to hosts can occur even in generalist parasites and does not require specialization on individual hosts. We discuss the role of potential mechanisms, including variation in chemical signalling (early) and in host defence (late effects).

The investigation of thermo-economic performance for dry-cooled closed Brayton cycle coupled to nuclear microreactors using different coolants

Dry-cooled closed Brayton cycle (CBC) coupled to gas-cooled nuclear microreactors offers a compact structure and can be deployed in water-deficient areas. The gas-cooled reactor utilizes coolants including supercritical carbon dioxide (S–CO2), helium, and He–Xe mixture. The choice of working fluids for CBC strongly affects its size and performance, where smaller plant sizes, higher thermal efficiency, and lower investments are preferred. This study investigates the thermo-economic performances of dry-cooled CBC coupled to nuclear microreactors using different coolants. A design method for dry-cooled CBC is developed based on the integration of thermodynamic modeling and component preliminary design. Baseline designs are first established, followed by an analysis of the effects of key design parameters. Furthermore, a multi-object optimization is conducted to compare the Pareto frontiers of CBC using different working fluids. The results demonstrate that the helium simple cycle exhibits superior efficiency and precooler size due to its higher reactor outlet temperature, though with a larger capital cost. The Pareto frontier of the He–Xe cycle is worse than that of the helium cycle. The S–CO2 recompression cycle has a comparable efficiency with a smaller capital cost at mediate reactor outlet temperatures but suffers from large precooler sizes. The findings revealed that the S–CO2 recompression cycle is a more promising choice if the reactor outlet temperature can be further elevated.

Seabird guano inputs increase impacts from introduced mammals on the native plants and animals of an oceanic island.

Seabirds create fluxes of nutrients from marine to terrestrial ecosystems that influence the food webs of small islands. We investigated how guano inputs shape terrestrial food webs by comparing species of selected plant and animal species in a red-footed booby colony in Mona Island (Puerto Rico, Caribbean Sea), to sites of the island lacking guano inputs. We quantified guano deposition and its relationship to plant biomass production, fecundity and density, as well as the activity of native and introduced animal species. In general, guano inputs increased the gross primary plant productivity, size, and fecundity by twofold. Guano inputs were also associated with twofold increases in density of Anole lizards, but also to increases in the activity of introduced pigs (> 500%), goats (> 30%), and cats (> 500%), which negatively impact native species. In particular, elevated pig and cat activity within the booby colony was correlated with lower activity of endemic ground lizards and of introduced rats. Our results also suggest that severe droughts associated with climate change exacerbate the negative effects that introduced species have on vegetation and reduce the positive effects of seabird guano inputs. Our findings underscore the importance of allochthonous guano inputs in subsidizing plant productivity and native and endemic species in small oceanic islands, but also in increasing the negative impacts of introduced mammals. Management and conservation efforts should focus on the exclusion (or eradication) of introduced mammals, particularly pigs and goats, from remnant seabird colonies in Mona Island.

Root and shoot phenology, architecture, and organ properties: an integrated trait network among 44 herbaceous wetland species.

Integrating traits across above- and belowground organs offers comprehensive insights into plant ecology, but their various functions also increase model complexity. This study aimed to illuminate the interspecific pattern of whole-plant trait correlations through a network lens, including a detailed analysis of the root system. Using a network algorithm that allows individual traits to belong to multiple modules, we characterize interrelations among 19 traits, spanning both shoot and root phenology, architecture, morphology, and tissue properties of 44 species, mostly herbaceous monocots from Northern Ontario wetlands, grown in a common garden. The resulting trait network shows three distinct yet partially overlapping modules. Two major trait modules indicate constraints of plant size and form, and resource economics, respectively. These modules highlight the interdependence between shoot size, root architecture and porosity, and a shoot-root coordination in phenology and dry-matter content. A third module depicts leaf biomechanical adaptations specific to wetland graminoids. All three modules overlap on shoot height, suggesting multifaceted constraints of plant stature. In the network, individual-level traits showed significantly higher centrality than tissue-level traits do, demonstrating a hierarchical trait integration. The presented whole-plant, integrated network suggests that trait covariation is essentially function-driven rather than organ-specific.

Techno-Economic Analysis of Using Reversible Turbomachinery for Pumped Thermal Energy Storage Systems

Abstract The objective of this paper is to assess the techno-economic performance of different cycle configurations for pumped thermal energy storage (PTES), including the effects of charging electricity costs. Reversible turbomachinery was employed to reduce the capital cost of the system. Brayton cycles with different working fluids and a subcritical Rankine cycle operating with ammonia were compared. Both liquid and packed bed thermal storages were investigated. A new cost correlation for turbomachines, initially established for the turbines of organic Rankine cycles, was developed for compressors and reversible machines. This correlation is based on the number of stages and physical size of the machine, which were estimated considering thermodynamic as well as mechanical limitations. The results indicate that for a plant size of 50 MW and a discharge duration of 8 h, the Brayton system with liquid storage and helium as a working fluid has the lowest levelized cost of storage at 0.138 $/kWh, mainly due to the high thermal conductivity of the fluid. Packed bed thermal energy storage systems were found to be more expensive than liquid storage systems due to the large cost of the pressure vessels, with cost parity reached at a discharge duration of 4 h. However, at this duration, lithium-ion batteries are likely to be cheaper. The results suggest that the levelized cost of storage for the Rankine cycle-based system is slightly higher at 0.151 $/kWh.

HyDesign: a tool for sizing optimization of grid-connected hybrid power plants including wind, solar photovoltaic, and lithium-ion batteries

Abstract. Hybrid renewable power plants consisting of collocated wind, solar photovoltaic (PV), and lithium-ion battery storage connected behind a single grid connection can provide additional value to the owners and society in comparison to individual technology plants, such as those that are only wind or only PV. The hybrid power plants considered in this article are connected to the grid and share electrical infrastructure costs across different generation and storing technologies. In this article, we propose a methodology for sizing hybrid power plants as a nested-optimization problem: with an outer sizing optimization and an internal operation optimization. The outer sizing optimization maximizes the net present values over capital expenditures and compares it with standard designs that minimize the levelized cost of energy. The sizing problem formulation includes turbine selection (in terms of rated power, specific power, and hub height), a wind plant wake loss surrogate, simplified wind and PV degradation models, battery degradation, and operation optimization of an internal energy management system. The problem of outer sizing optimization is solved using a new parallel “efficient global optimization” algorithm. This new algorithm is a surrogate-based optimization method that ensures a minimal number of model evaluations but ensures a global scope in the optimization. The methodology presented in this article is available in an open-source tool called HyDesign. The hybrid sizing algorithm is applied for a peak power plant use case at different locations in India where renewable energy auctions impose a monetary penalty when energy is not supplied at peak hours. We compare the hybrid power plant sizing results when using two different objective functions: the levelized cost of energy (LCoE) or the relative net present value with respect to the total capital expenditure costs (NPV/CH). Battery storage is installed only on NPV/CH-based designs, while the hybrid design, including wind, solar, and battery, only occurs on the site with good wind resources. Wind turbine selection on this site prioritizes cheaper turbines with a lower hub height and lower rated power. The number of batteries replaced changes at the different sites, ranging between two or three units over the lifetime. A significant oversizing of the generation in comparison to the grid connection occurs on all NPV/CH-based designs. As expected LCoE-based designs are a single technology with no batteries.

Flow similarity model predicts allometric size dependence, curvature, and covariation of 285 North American tree species

AbstractScaling patterns in plants have long interested biologists, particularly whether different species share similar patterns of growth, and whether differences in growth trajectories depend on plant size. Using 8,794,737 measurements for 285 species from the U.S. Forest Inventory and Analysis database, we test several predictions emerging from a recently published “flow similarity” model for plant growth and allometry. We show that the model's predicted curvature for intraspecific relationships between height, dbh, and biomass is found in 88.1% of examined cases, and empirical slopes fall as predicted between the elastic similarity and flow similarity predictions in 71.1% of cases. We also find a strong size dependence in observed intraspecific allometric exponents, with large species, particularly gymnosperms, converging near the expectation for elastic similarity and the central tendency among small species approaching the expectations for flow similarity in most cases. Our results support the idea that differences in growth patterns across plant species depend on plant size and their attendant hydraulic and/or biomechanical demands and helps to delineate the bounds of the theoretical morphospace in which they occur.

Enhancing Freeze Tolerance of Field-grown Lettuce with Salicylic Acid, Ascorbic Acid, and Calcium Chloride

With the climate continuing to change, specialty crop growers are particularly at risk for economic loss caused by erratic weather conditions, excess heat and rain, and frost damage. Although tolerant to cold, lettuce (Lactuca sativa) is a major horticultural crop at risk for exposure to freezing temperatures in late fall or early spring in the Midwest. Exogenous applications of salicylic acid, ascorbic acid, and calcium chloride have been shown to improve abiotic stress in plants, particularly with freezing tolerance. This research investigated the effects of the exogenous application of salicylic acid, ascorbic acid, and calcium chloride, in varying concentrations, on field-grown lettuce. The study was conducted at the Iowa State University Horticulture Research Station, Ames, IA, USA. Weekly applications of salicylic acid (0.5 and 1.0 mM), ascorbic acid (0.5 and 1.0 mM), and calcium chloride (10 and 20 mM) were applied until plants were a marketable size. A control treatment (no spray) was also included. Data regarding plant size, yield, leaf area, leaf number, plant dry weight, plant nutrient analysis, and freeze tolerance were collected. Freeze tolerance assessments were conducted through laboratory-simulated freeze events and the evaluation of natural in-field freeze events. Freeze injury was quantified through the electrolyte leakage method. Marketable weights in 2020 and 2021 were statistically similar, except for 1.0 mM salicylic acid in 2020, which showed a significantly lower marketable weight and head diameter. Trends during both years showed that stress protectant applications had the most effective freeze protection at −12 °C in laboratory-simulated freeze events. Calcium chloride at 20 mM had the highest protection at −12 °C, with 13.3% and 24.0% less injury compared with the control in 2020 and 2021, respectively. Salicylic acid 1.0 mM at −12 °C had 4.7% and 23.7% less injury compared with control during these two years, respectively. Ascorbic acid 1.0 mM at −12 °C also showed 6.8% and 9.5% less injury than the control in 2020 and 2021, respectively. Similar trends were observed after in-field freezing events, with 20 mM calcium chloride providing the highest protection against frost in 2020 and 2021. These findings advance the understanding of the capabilities of stress protectants on lettuce as chemical primers after freezing events. This research highlights the benefits of ascorbic acid, salicylic acid, and calcium chloride as stress protectants and encourages future research and further exploration of concentrations, methods of application, and timing of application of products.

Feasibility analysis and modeling of a solar hybrid system for residential electric vehicle charging

The process of transforming sunshine energy into electrical power is known as solar power generation. Photovoltaic (PV) technology has recently proved its cost-effectiveness and low environmental impact in generating power. The key goals of this study are to develop a solar PV system for charging electric vehicles (EVs) while utilizing the residential apartment's current domestic power supply. This study focuses on modeling grid-interactive solar PV systems for charging EVs inside a 40-unit residential apartment complex. The Solar Pro tool is used to do the techno-economic analysis of the modeled PV system. The research investigates the installation of a rooftop solar plant devoted to delivering electricity to EV charging devices on a real-time five-story residential building. The performance of the PV plant is tested under a variety of scenarios, including EV loading, shadow mapping, and local meteorological conditions. The PV plant's size is optimized at 150 kW, taking into consideration economic aspects as well as the actual proportions of the structure. In addition, the MiPower tool is used to do a load flow study of the modeled system, which includes both the grid and the PV system. This research evaluates line losses, line loading, and voltage levels at each bus at maximum loading circumstances.

A New Gigantic Vicia (Perennial Wild Vetch) (Fabaceae) Taxon From Eastern Anatolia, Türkiye

vetch), which has been collected from the Karakoçan district (Elazığ-Türkiye), has been defined as new subspecies for the scientific world. New subspecies; it differs from the typical subspecies primarily in terms of indumentum characteristics, large plant size, leaf and leaflet sizes, inflorescence features, inflorescence/leaf length ratio, flower parts and seed sizes.Vicia freyniana subsp. gigantea grows mostly in oak forest shades and sometimes in oak forest openings. This newly defined subspecies; description, ecological preferences, distribution map and photographs were given.

Natural selection by pollinators on floral attractive and defensive traits did not translate into selection via fruits in common milkweed

Considering both pollinator and herbivore pressures on plant reproductive and defensive traits is key to understanding patterns of selection for plants. However, phenotypic selection studies connecting floral traits and plant defenses with pollinator activity and herbivore damage remain rare. We used the common milkweed, Asclepias syriaca (Apocynaceae), to study phenotypic selection on attractive and defensive traits, and nectar rewards. We measured herbivore (leaf damage) and pollinator activity (pollinia movement) and quantified selection via female (pollinia insertions and fruit number) and male fitness (pollinia removals). We found selection to increase plant and inflorescence size and to decrease floral size (i.e. petal width) via female fitness. We also detected selection to increase floral but not leaf latex. The lack of selection on leaf latex was congruent with the low herbivory observed, however we also did not observe florivory in the population that would explain the advantage of more floral latex. Interestingly, we found selection on attractive traits differed via pollinia insertions and fruits initiated, suggesting that something other than pollinators was driving selection via fruit production. In contrast to female fitness, we did not find selection on any trait through male fitness, suggesting no sexual conflicting selection, at least through these proxies. Our findings reinforce the importance of the direct assessment of pollinator pressures in phenotypic selection studies before assuming pollinators as drivers of floral evolution by natural selection. Further work in southern populations closer to the centre of the species range, where herbivory and plant defense investment are higher, may help elucidate selection on attractive and defensive traits.

Fire effects on the reproductive potential of two dominant woody species along an elevation gradient in central Argentina

Fire and elevation affect reproduction of woody species and, therefore, their distribution in the landscape; however, the effect of both factors are often confounded. In addition, plant size may also influence reproduction. Our objective was to investigate the combined effects of fire, elevation and pre-fire size on woody species reproductive potential in a seasonally dry mountain ecosystem. We tested the predictions that fire and elevation impair reproductive potential and that the negative effects of fire are enhanced at high elevation. Our study was carried out in the mountains of central Argentina, from 800 to 1700 m a.s.l. We selected individuals of two dominant resprouting woody species (Vachellia caven and Lithraea molleoides) in burned and comparable unburned areas. We estimated pre-fire size and fire damage level in each tree. We monitored parameters related to reproductive potential during four post-fire fruiting seasons in 185 V. caven and 78 L. molleoides trees. Our main results for V. caven show negative fire effects on most reproductive parameters, and on the expected number of germinable seeds per tree, which was estimated as an integrated parameter. The negative fire effects decreased over time until the fourth post-fire fruiting season, when we detected almost no fire effects. Moreover, the negative fire effects were greatly enhanced with elevation, while pre-fire size showed consistently positive effects on most reproductive parameters. For large trees located at low elevation, fire reduced the number of germinable seeds per tree by a factor of nearly six in the third post-fire fruiting season (from 2020 to 358 seeds), while at high elevation fire reduced germinable seeds by a factor of 15 (from 106 to 7 seeds) for similarly large trees. Small trees had a lower reproductive potential than large trees and the negative effects of fire were smaller but noticeable, and were enhanced by elevation, as in the case of large trees. For L. molleoides we found similar patterns to those of V. caven; however, since few trees produced seeds during the study, we did not compute formal statistics. We conclude that fire reduction should gradually increase reproductive potential, tree density and forest cover at high elevation, even when, according to previous studies, survival is not affected by fire or elevation in the study species.

Techno-economic analysis of electron beam irradiation pretreatment of corn straw for anaerobic digestion

The complexity of anaerobic digestion process and the risks of investing in new technologies are the main obstacles to improving anaerobic digestion technology. There is still a gap between research results and commercial applications. This study investigated the techno-economic feasibility and bottlenecks of introducing electron beam irradiation pretreatment technology into the commercial-scale anaerobic digestion plant. Model construction, parameter initialization and economic analysis were performed in SuperPro Designer software. The parameters used in the model were derived from experimental results, software built-in model and recent literature. The results show that the gross margin, return on investment, payback time, internal rate of return, and net present value of the system with electron beam irradiation pretreatment are 4.4 %, 9.8 %, 10.1 years, 8.0 % and $0.6 million, respectively. The corresponding values of the system without electron beam irradiation pretreatment are 9.9 %, 11.1 %, 8.9 years, 9.7 % and $1.5 million, respectively. The main factor affecting gross profit is tipping fee of liquid anaerobic digestion effluent, followed by plant size of anaerobic digestion, degradation efficiency of corn straw, and compost price. This study has guiding significance for the application of electron beam irradiation pretreatment technology in the production of renewable energy from lignocellulosic biomass.

Hemp cultivation opportunities for marginal lands development.

Agricultural diversification and high-quality products deriving from sustainable crops such as hemp can represent a solution to revitalize marginal areas and reverse land abandonment. This study aimed at comparing four different hemp cultivars (Carmagnola Selezionata, "CS"; Futura 75, "FUT"; Felina 32, "FEL"; Secuieni Jubileu, "JUB") to provide information to select the best suited cultivar for cultivation in mountain marginal areas and for specific end-use applications. Hemp cultivars were cultivated in a single experimental field to compare their ecological and agronomic behavior (duration of life cycle phases, plant size and biomass allocation, and plant resource-use strategies). Through metabolomic analysis of both vegetative and reproductive parts of the plants we tested the presence of substances of nutraceutical interest and traced seed nutritional profile. The four cultivars had different ecological and agronomic behavior, and nutritional profile. We found several compounds with potential pharmaceutical and nutraceutical values in all parts of the plant (leaves, inflorescences, and stems). JUB resulted the most suitable for seed production while CS showed the highest content of bioactive compounds in flowers and leaves. FUT, showed the best suitability for multi-purpose cultivation, while FEL seemed to be not appropriate for the cultivation in mountain area. The multi-disciplinary approach we adopted was effective in distinguish across hemp cultivars and provided information to farmers for the selection of the best hemp cultivar to select. Hemp had a high potential for cultivation in marginal lands, demonstrating to be an economic resource due to its multi-purpose use and to the possibility to generate high-added values products. Our results could serve as a stimulus for the reintroduction of this culture in the study area and in other similar environments.

Vegetation profile and ecological traits in irrigated plains of semi-arid regions: A case study of Okara, Punjab Pakistan

Comprehensive botanical assessments are pivotal for understanding the intricate world of plant biodiversity. These assessments serve multifaceted purposes, from cataloging significant plant species within a region to aiding in conservation, monitoring protected areas, and providing valuable data for various scientific disciplines. The primary objective was to assess the vegetation profile and ecological traits in this region, conduct a comprehensive floristic inventory, categorize species, and investigate adaptability to semi-arid conditions within the irrigated plains of Okara, Punjab, Pakistan. Comprehensive botanical assessments were conducted to document the floristic composition and ecological characteristics of the region. A total of 135 plant species, belonging to 118 genera and 46 families, were identified. Notably, Poaceae and Fabaceae were the most prominent families. The study revealed diverse growth habits, with herbaceous plants being the most abundant. Life form analysis indicated Therophytes as the dominant category, and Microphylls were the most prevalent leaf type. Flowering phenology analysis highlighted two major flowering periods, primarily influenced by plant habit and size. Habitat-wise distribution demonstrated variations in species preferences, with unique associations observed in forest, vegetable, maize, paddy, and mustard fields. Invasive species accounted for 16.93% of the flora, with several herbs, shrubs, and trees identified as invasive. These findings contribute valuable insights into the botanical diversity and ecological traits of the Okara region, with implications for biodiversity conservation and land management strategies.

Apomixis and the paradox of sex in plants.

The predominance of sex in eukaryotes, despite the high costs of meiosis and mating, is still an evolutionary enigma. Many theories have been proposed, none of them being conclusive on its own, and they are partly not well applicable to land plants. Sexual reproduction is obligate in embryophytes for the great majority of species. This review will compare the main forms of sexual and asexual reproduction in ferns and angiosperms, based on the generation cycling of sporophyte and gametophyte (leaving vegetative propagation aside). The benefits of sexual reproduction for maintenance of genomic integrity compared to asexuality will be discussed in the light of developmental, evolutionary, genetic and phylogenetic studies. Asexual reproduction represents modifications of the sexual pathway, with various forms of facultative sexuality. For sexual land plants, meiosis provides direct DNA repair mechanisms of oxidative damage in reproductive tissues. The ploidy alternations of meiosis-syngamy cycles, and prolonged, multicellular stages in the haploid phase in the gametophytes provide a high efficiency of purifying selection against recessive deleterious mutations. Asexual lineages might buffer effects of such mutations via polyploidy, and can purge the mutational load via facultative sexuality. The role of organelle-nuclear genome compatibility for maintenance of genome integrity is still not well understood. The costs of mating are in plants in general low because of predominant hermaphroditism. Phylogenetic patterns in the Archaeplastid clade suggest that high frequencies of sexuality in land plants are concomitant with a stepwise increase of intrinsic and extrinsic stress factors. Furthermore, expansion of genome size in land plants would increase the potential mutational load. Sexual reproduction appears to be essential for keeping long term genomic integrity, and only rare combinations of extrinsic and intrinsic factors allow for shifts to asexuality.

Influence of façade orientation, floor height, substrate pH, and microbial inoculation on woody plants’ performance in vegetated façades in Southern Finland

Urban densification has led to the adoption of vegetated façades as a nature-based strategy to increase urban green spaces and enhance urban living conditions. However, limited knowledge regarding suitable plants for vegetated façades can impede the process. In this field experiment conducted in Southern Finland, we investigated the performance of 12 potential plant taxa for a vegetated façade by assessing their visual appearance and sizes in relation to façade orientation, floor height, substrate pH, and mycorrhizal inoculation. The 12 plant taxa were categorized into 4 growth forms according to their morphologies: spreading conifers, dwarf conifers, creeping conifers, and climbers. We aimed to evaluate plant performance during the initial two growing seasons, which is critical for the successful establishment of these plants in vegetated façades. Eight of the twelve plant taxa exhibited relatively high performance in terms of visual appearance and size. Notably, creeping conifers outperformed other growth forms in visual appearance, which was most likely due to their close-to-ground morphology. Façade orientation and substrate pH were the most influential predictor variables. Façade orientation affected both plant visual appearance and size, while substrate pH primarily affected plant visual appearance. The east-facing façade (less exposure to sun and wind) and mildly acid substrate (pH 6–6.5) were more conducive to most of the plant taxa. Certain species and cultivars, such as Juniperus communis ‘Lalli’, maintained constant plant visual appearance and size regardless of façade orientation and substrate pH, suggesting their adaptability and stability across various conditions. Floor height and mycorrhizal inoculation only displayed marginal and taxon-specific impacts. Given that some plants in our vegetated façade exhibited optimal performance different from those in their reported natural habitats, we encourage conducting long-term, on-site experiments to identify suitable plants for vegetated façades to ensure successful vegetated façade implementation.

Up-regulation of non-photochemical quenching improves water use efficiency and reduces whole-plant water consumption under drought in Nicotiana tabacum.

Water supply limitations will likely impose increasing restrictions on future crop production, underlining a need for crops that use less water per mass of yield. Water use efficiency (WUE) therefore becomes a key consideration in developing resilient and productive crops. In this study, we hypothesized that it is possible to improve WUE under drought conditions via modulation of chloroplast signals for stomatal opening by up-regulation of non-photochemical quenching (NPQ). Nicotiana tabacum plants with strong overexpression of the PsbS gene encoding PHOTOSYSTEM II SUBUNIT S, a key protein in NPQ, were grown under differing levels of drought. The PsbS-overexpressing lines lost 11% less water per unit CO2 fixed under drought and this did not have a significant effect on plant size. Depending on growth conditions, the PsbS-overexpressing lines consumed from 4-30% less water at the whole-plant level than the corresponding wild type. Leaf water and chlorophyll contents showed a positive relation with the level of NPQ. This study therefore provides proof of concept that up-regulation of NPQ can increase WUE, and as such is an important step towards future engineering of crops with improved performance under drought.

Begonia tripurensis (Begoniaceae, sect. Platycentrum), a new species from Tripura, Northeast India

A new species of Begonia sect. Platycentrum is described here from the moist deciduous forest of Tirthamukh near Dumboor Lake, Tripura, India. It is similar to B. scintillans, B. chindwinensis and B. thomsonii but differs in plant size, hair characters, petiole length, flowers, stamens and fruits. A description with coloured photographs and information on the phenology and habitat are provided.