Fine cell suspension cultures with plant regeneration ability were established from leaf-like structures which were produced on friable green calli originated from an immature embryo of rose (R. hybrida cv. Gelb Dagmer Hastrap×R. chinensis var. mutabilis). The leaf-like structures were initially cultured in liquid MS medium containing 2mg/l 2, 4-D for inducing calli, which were then transferred to the medium containing a reduced concentration of 2, 4-D (0.01mg/l) for establishing rapidly proliferating fine cells. The cells thus obtained showed the ability to regenerate embryo-like structures on gellan gum-solidified MS medium containing 1mg/l BA. The embryo-like structures developed into normal plantlets through adventitious shoot formation on MS medium containing 0.1mg/l TDZ. The plantlets were successfully established on the soil.