Abstract
Summary The non-regenerable callus tissues ofZea mays(maize),Fagopyrum esculentumandF. tataricum(buckwheat) were stained more extensively by KMnO4than were regenerable tissues. The component which caused the KMnO4reduction was extractable in 1 mol L-1KOH and could be precipitated from extracts from cell walls of both regenerable and non-regenerable tissues. Electron microscopic examination of tissues fixed with KMnO4showed an electron dense component mainly in the middle lamella in non-regenerable callus and in the cell junctions in regenerable callus. When phenolaldehyde products produced by CUSO4-NaOH oxidation of cell walls from the three species, andFragaria chiloensis(strawberry), were analyzed by gas chromatography, the regenerable tissues contained very high levels of vanillin which may have been produced from ferulic acid. Less extensive oxidation produced less vanillin while some ferulic acid was still present. Ferulic acid could be extracted with KOH from cell walls of regenerable and not from non-regenerable buckwheat and strawberry callus. The results indicate that alterations in phenolic compounds in the cell walls of callus tissues of several species correlate with the developmental capability of the tissues.
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