Extracts Of Plant Parts Research Articles

Antibacterial Activity and Anti-inflammatory Activity VitexNegundo (Nirgudi) Plant Extract

Vitexnegundo is commonly known as Nirgundi. It belongs to family Verbenaceae. Its plant is branched, deciduous shrub found in all parts of India. Nirgundi possess various medicinal properties. It is also well known to possess antibacterial properties. The aim of present work is to evaluate the antibacterial property of Nirgundi plant parts. Themethanol extract of Nirgundi stem, leaves and flowers were investigated against Escherichia coli and Staphylococcus aureus. It was reported in the study that the methanol extract of Nirgundi plant parts possess potent antibacterial property compared with the Streptomycin as a standard.Therefore, it is suggested to develop alternative antimicrobial drugs for the treatment of infectious diseases and Nirgundi plant parts can be used more and more for commercial purpose. The present study was undertaken to assess the anti-inflammatory effect of ethanolic extract of Vitexnegundo roots in rats. The anti-inflammatory action was studied by Plethysmometer method. The ethanolic extract of Vitexnegundo roots was screened for phytochemical analysis and revealed the presence of all components. The adult rats were divided into four groups of six each and maintained under ideal laboratory conditions. Group I was taken as control and group II treated with the standard drug Indomethacin (10 mg/kg), the ethanolic extract of Vitexnegundo root 200 mg/kg and 400 mg/kg were fed to group III and IV. It is observed that the ethanolic extract of Vitexnegundo roots shows considerable anti-inflammatory effect by using carrageenan induced rat paw edema method. The higher dose groups of Vitexnegundo root extract (400 mg/kg) were revealed more activity than their corresponding lower dose

Waste upcycling of Sapota peels as a green route for the synthesis of silver nanoparticles and their application as catalytic and colorimetric detection of Co2+ and Hg2+

Biochemical synthesis of nanoparticles (NPs) using plant part extracts as capping and reducing agents has drawn considerable attention in research with a growing focus on green chemistry. The present study utilized Sapota (Manilkara zapota L.) peel extract to synthesize silver nanoparticles (SP-AgNPs) using ultrasonic vibration. Different characterization techniques such as UV-vis spectroscopy, dynamic light scattering, Fourier Transform Infrared Spectroscopy, Field emission scanning electron microscope, High resolution transmission electron microscopy, and X-ray diffraction were employed to check the production of SP-AgNPs. The AgNPs were crystalline in nature and had an average particle size of 27.906 nm. The research primarily focused on two aspects: the catalytic activity of SP-AgNPs in degrading environmental pollutants and their ability to act as colorimetric sensors for toxic metal ions. SP-AgNPs exhibited significant catalytic activity in the decomposition of various pollutants such as Methyl Orange (0.035 ± 0.090 min−1, 92.89 ± 1.79%), Crystal Violet (0.1097 ± 0.1016 min−1, 85.56 ± 2.21%) and Cosmic Brilliant Blue G-250 (0.0697 ± 0.0275 min−1, 79.56 ± 1.80%). The high degradation percentages and reaction rate constants indicate the efficiency of SP-AgNPs in pollutant degradation. Additionally, the study demonstrated the effectiveness of SP-AgNPs as sensors for detecting toxic metal ions, particularly Co2+ and Hg2+ with limits of detection 54.40 ± 1.43 µM and 10.70 ± 0.16 µM. With impressive sensitivity and low detection limits, SP-AgNPs showed promise in detecting these ions, which are often found in environmental contaminants. Moreover, their plant-based synthesis, low toxicity, and cost-effectiveness make them attractive options for environmental remediation efforts.Graphical abstract

Investigation of Flower, Leaf, and Stem Parts of Three Gundelia Species Growth in the Eastern Anatolia Region of Türkiye from a Biochemical Perspective

13 species of Gundelia species plants are endemic in Türkiye and are frequently used by the public for both nutrition and medicinal objectives. In this study, three parts (flower, leaf, and stem) of three species of Gundelia (G.dersim, G.glabra, and G.munzurensis) were biochemically investigated. DPPH and ABTS analyses for antioxidant activity were performed on methanol extracts of plant parts. As a result of both analysis in parallel, the flower part of G.munzurensis had the highest %Inhibition value (DPPH:91,85±0,78; ABTS:97,38±0) (P

Efficacy of Neem Oil against Termite [Odontotermes obesus (Rambur)] (Blattodea: Termitidae)

Botanical pesticides are of great importance in Integrated Pest Management (IPM). Extracts of different plant parts are used for deterring and killing the various life stages of insects. Most of the oils produced by plants contain bioactive compounds with insecticidal properties. Neem oil containing azadirachtin is an established insecticide and insect repellent. Use of synthetic pesticides for wood preservation leads to environmental pollution and health hazards to human beings and other living organisms. To avoid these problems neem, Azadiracta indica (A.Juss.) plant oil was used in this study and gave better results. The weight loss of card boards treated with neem was less like chlorpyrifos treated cardboards. Gravimetric measurements of cardboards shown 18.34gm and 16.85 gm weight which after 30 and 60 days of exposure to A. indica oil indicated that plant oil has potential effects like conventional pesticides like chlorpyrifos which showed the18.72 and 18.63 gm of weight in cardboards after 30 and 60 days of exposure. The P values was 0.036457 and 0.016527 for cardboards exposed to two treatments after 30 and 60 days respectively which is highly significant.

Exploring the wound healing activity of phytosomal gel of Annona squamosa and Cinnamomum tamala leaves ethanolic extracts with antioxidant and antimicrobial activities in S aureus infected excision wound model

Wound healing is a natural process but it is impaired in certain conditions like age, stress, health, immunity status and microbial infection. Particularly in cases of chronic wounds, infection is nearly often the main and unavoidable obstacle to wound healing. For this purpose, leaves of Annona squamosa and Cinnamomum tamala were selected based on their ethnopharmacological uses and reported pharmacological activities. The ethanolic extracts of both plant parts i.e. ethanolic extracts of Annona squamosa (ASEE) and Cinnamomum tamala (CTEE) were evaluated for their antioxidant and antimicrobial activities individually as well as in 1:1 combination as Polyherbal Ethanolic extract (PHEE). In our previous work both these ethanolic extracts were combined and phytosomes were prepared by thin layer hydration method and optimized for vesicle size and entrapment efficiency. The phytosomes were then incorporated into Carbopol gel matrix. In this present study the selected phytosomal gel was tested in two different concentrations (2% and 5%) for in vivo wound healing activity using S. aureus infected excision wound model. The various parameters examined were percentage wound contraction, epithelization period, bacteriological quantification, biochemical parameters like Superoxide dismutase (SOD), Catalase and hydroxyproline. The PHEE exhibited synergistic antioxidant activity. The PHEE also showed enhanced antimicrobial activity against bacteria namely gram-positive S. aureus, gram-negative E. Coli. The phytosomal gel showed increased wound contraction, reduced time of epithelization, increased hydroxyproline content, increased levels of SOD and Catalase enzymes and reduced bacterial load when compared with Povidone iodine ointment as standard in S. aureus infected excision wound model.

Phytochemical analysis and the first report of decyl gallate and 4-ethoxycoumarin from marine seagrass Posidonia oceanica collected from Benghazi beach Libya

The goal of this research is to examine the chemical composition of the plant and identify the phytochemical constituents of the endemic seagrass species in the Mediterranean Sea, Posidonia oceanica, which has historically been used for a variety of purposes. After doing chemical screening, the existence of triterpenoid and phenolic proteins was discovered. Using preparative thin-layer chromatography and flash column chromatography techniques, seven phenolic compounds were isolated from the methanolic extracts of several plant parts. Namely decyl gallate, sinapinic acid, alpha-methyl cinnamic acid, acetosyringone, and chlorogenic acid from leaves, 4-ethoxycoumarin from rhizomes, and syringic acid from roots. The structures of these compounds were elucidated using spectroscopic methods such as ‘H and 13C-NMR. The spectra were analyzed and visualized using Mnova software and compared with standard spectra from the Willy spectra database, biological magnetic resonance data bank, and chemical book. This is the first report of decyl gallate and 4-ethoxycoumarin from the plant. This study contributes to the understanding of the phytochemical profile of Posidonia oceanica and provides valuable insights into its potential applications in various fields, including medicine, agriculture, and industry.

Attractant and repellent properties of Senna didymobotrya plant extracts to Amblyomma variegatum and Rhipicephalus appendiculatus

The growing challenge of acaricide resistance and geographical range expansion of invasive tick species demands other interventions, like plant-based alternatives, for sustainable tick control. Leaves, flowers, seedpods, and twig branch extracts of Senna didymobotrya were analyzed using coupled gas chromatography mass spectrometry (GC-MS). Response of adult Amblyomma variegatum and Rhipicephalus appendiculatus to extracts was evaluated. The most attractive plant extract was fractionated and ticks’ responses to its fractions assessed. Potential tick attractants in the attractive plant part extract and its fractions were identified by GC-MS analysis. Non- significant qualitative and quantitative differences were observed in the plant parts’ extract composition (R = 0.6178). Flower extracts attracted both species, with a 0.1-fold higher attraction in A. variegatum compared to the standard attraction aggregation attachment pheromone (AAAP). Leaf and seedpod extracts repelled ticks at various concentrations. Bioassays after fractionating flower extracts identified hexane and ethyl acetate fractions as most attractive to A. variegatum (P < 0.001) and R. appendiculatus (P < 0.001), respectively. Chemical analysis of the most attractive extracts and fractions identified compounds, including documented acarine attractants, squalene and linoleic acid. A squalene and linoleic acid blend (1:1) at 1 mg/mL significantly attracted adult A. variegatum (P < 0.01) and R. appendiculatus (P < 0.001). The results of this study broaden comprehension of how ticks respond to plants in nature, and showcase the promising potential for integrating these insights into effective tick management programs.

Antimycobacterial, hepatoprotective and cytotoxicity effects of selected plant species from the Menispermaceae family

Tuberculosis, caused by Mycobacterium tuberculosis, is a worldwide disease affecting millions of people. The rise of resistant strains, coupled with toxicity of current chemotherapy, requires effective alternatives against mycobacterial infections. Some plants from the Menispermaceae family have been used to treat tuberculosis and cough related symptoms. In this study, acetone, methanol:water (4:1), dichloromethane:methanol (1:1) and hot water extracts of different plant parts of Cissampelos owariensis, Cissampelos mucronata and Tinospora fragosa were tested against M. aurum, M. bovis, M. fortuitum, M. smegmatis and M. tuberculosis using a two-fold serial microdilution assay. Cytotoxicity of the active extracts was determined against Vero and HepG2 cells. The hepatoprotective effect of the active extracts was evaluated using rifampicin and acetaminophen as toxic drugs against HepG2 cells. The hot water leaf extracts were most active with promising minimum inhibitory concentration (MIC) values of 20 and 40 µg/mL against M. smegmatis and M. fortuitum respectively. The acetone extracts of C. owariensis, C. mucronata and T. fragosa had the lowest MIC values (0.03 – 1.67 mg/mL). The root extract of C. owariensis was not toxic to Vero cells while the leaf extract was more toxic. The acetone extract of C. mucronata leaves was toxic to Vero cells but the other extracts had low toxicity. The active leaf and root extracts had protective effects on rifampicin-induced toxicity on HepG2 cells. The root extract also had a protective effect on acetaminophen-induced toxicity on HepG2 cells but the leaf extract had no protective effect. The hot water extracts of C. owariensis, C. mucronata and T. fragosa had a more protective effect on the toxin-induced cells than the acetone extracts. These results support further investigation on the bioactive compounds in these plant extracts.

Assessment of antibacterial activity and cytotoxic effects of in vitro and in vivo plant parts of a medicinal plant Gynura procumbens (Lour.) Merr.

The goal of this study was to evaluate the antibacterial and cytotoxic effects of both the in vitro and in vivo plant part extracts of the medicinal plant Gynura procumbens. An effective protocol for regeneration and callus formation was developed using nodal segments and regenerated leaf explants, respectively. The highest fresh and dry weight calli were produced after four weeks of culture on Murashige and Skoog (MS) medium containing 2.0 mg/L BAP and 2.0 mg/L NAA, while the most shoots were produced on MS medium containing 1.0 mg/L BAP and 0.5 mg/L IAA. The in vitro shoots developed roots on MS media with 0.1 mg/L IBA. The antibacterial activity of extracts against various bacteria was examined to determine their significance (p < 0.05). The least significant difference (LSD) test results showed that the regenerated leaf extract had the highest antibacterial activity while the callus extract had the lowest. The minimum bactericidal concentration (MBC) and the minimum inhibitory concentration (MIC) were also established. Regenerated leaf extract had the highest toxicity and the lowest lethal concentration (LC50) value (1.21 ± 0.03 μg/mL) in a brine shrimp lethality bioassay. In contrast, callus extract had the lowest toxicity and the highest LC50 (11.09 ± 0.4 μg/mL). In addition, the in vitro cytotoxicity test results revealed that the callus and field leaf extracts had anti-cell-proliferative properties. The regenerated leaf and stem extract, however, could induce cell growth.

Assessment of toxicidal and repellency activity of extracts from aerial parts of Prunus laurocerasus for controlling broad mite, Polyphagotarsonemus latus

ABSTRACTDifferent plant part extracts of cherry laurel [Prunus laurocerasus (Rosaceae)] were investigated for the acaricidal and repellent activity against Polyphagotarsonemus latus (Prostigmata: Tarsonemidae). The leaf, flower, and seed aqueous extracts of cherry laurel were prepared for efficacy bioassays. The tested concentrations of each extract were 1% (volume/volume, v/v), 5% (v/v), 10% (v/v), 20% (v/v) and 30% (weight/volume, w/v). Distilled water and a mixed formulation of 228.6 g/L spiromesifen + 11.4 g/L abamectin were used as negative and positive controls, respectively. It can be concluded that the flower and leaf extract of P. laurocerasus at 30% concentration and the seed extract at 10% or greater concentrations have the potential to control P. latus in the egg stage. Although spiromesifen+abamectin caused extremely low egg mortality (9.5%), all larvae died shortly after hatching (indirect ovicidal effect). In contrast, all adult mites died within 20 hours after spraying. Moreover, the adult mortalities (92%-98%) associated with seed extract at 30% during the experimental period, and the leaf (93.78%) and flower (91.11%) extract at 30% 72-h after exposure were statistically similar to that of spiromesifen+abamectin (100%) application. All extracts at 20% and seed extract at 10% or greater concentrations may also have a great potential for repelling P. latus.

Cytological Effects of Two Varieties of Lathyrus Sativus L. Extracts on Onion Root Tip Cells

Lathyrus sativus L. causes lathyrism both in animals and human due to the presence of the neurotoxin β-ODAP (β -N-Oxalyl-L-α, β -diaminopropionic acid). This study was performed to find out the toxic effect of grass pea on onion root tip cells. The root lengths of onion were reduced as the concentrations of different plant parts extracts of grass pea increased. The percentages of chromosomal abnormalities, NV and ICV of onion root tip cells showed an increasing trend with the increasing of different extracts concentrations. The mitotic index and chromosomal abnormalities in treated onion root tip cells were significantly different than that of control. The mitotic index and chromosomal abnormalities had a reverse relationship. Almost all the treated root tip cells of onion showed this mito-depressive effect. From this findings, it may concluded that β -N-Oxayl-L- α, β-diaminopropionic acid (Lathyrogens) in Lathyrus sativus may be responsible for mitotic depression and chromosomal abnormalities on the root tip cells of onion. J. Bio-Sci. 31(1): 51-60, 2023

Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India.

Antibiotic resistance poses a serious threat to public health, raising the number of diseases in the community. Recent research has shown that plant-derived phenolic compounds have strong antimicrobial, antifungal, and cytotoxic properties against a variety of microorganisms and work as great antioxidants in such treatments. The goal of the current work is to evaluate the anticancerous, antibacterial, antifungal, antioxidant, and cytotoxicity activities in the extracts of the different plant parts (leaves, stems, and roots) of S. carvifolia (L.) L. This is a medicinally important plant and has been used for different kinds of diseases and ailments such as hysteria and seizures. The phenolic compounds from the different plant parts were analyzed using HPLC and the following were found to be present: chlorogenic acid, gallic acid, rutin, syringic acid, vanillic acid, cinnamic acid, caffeic acid, and protocatechuic acid. Gallic acid was found to have the highest concentration (13.93 mg/g), while chlorogenic acid (0.25 mg/g) had the lowest. The maximum TPC value, which ranged from 33.79 to 57.95 mg GAE/g dry extract weight, was found in the stem. Root extract with 9.4 mg RE/g had the greatest TFC level. In the leaf and stem extracts, the RSC ranged from 0.747 mg/mL to 0.734 mg/1 mL GE/g dry extract weight, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measure in vitro antioxidant activity. In a concentration-dependent way, promising antioxidant activity was reported. Moreover, 3,5-dinitrosalicylic acid (DNSA) and the Folin-Ciocalteu phenol reagent technique were used to determine reducing sugar content and total phenolic content, respectively. Antibacterial activity against eight strains (MIC: 250-1,000 μg/mL) was analyzed, and the stem extract exhibited maximum activity. Antifungal activity was also assessed, and potent activity was reported especially in the extract obtained from the stem. Cytotoxicity was evaluated using an MTT assay in the A549 cell line, where different doses (0.0625, 0.125, 0.25, 0.5, and 1 mg/mL) of leaf, root, and stem extracts were used. Treatment with these extracts reduced the cell viability, indicating that S. carvifolia may possess anticancer potential, which can be of great therapeutic value.

Formulation and Evaluation of Poly Herbal Mouth Wash against Different Mouth Disorder

Abstract: Our oral cavity is a sweet able place to grow different types of bacterial species either harmless or harmful for human. Many of us are suffering different types of mouth disease and feeling insecure or hesitate to speak with another due to the unpleasant odor of mouth. To maintain a good oral hygiene mouthwash plays a important role in our daily lifestyle. From ancient age medicinal plants are considered as a store room of different types of biological activity in Ayurveda, Unani and Siddha, and have important role to cleanse tooth and prevent different human pathogens are responsible for unpleasant odor, inflammation of teeth root, dental plaque. Herbal mouthwash contains different medicinal plant part extract have various pharmacological activities and shows significant advantages which helps to cure different types of oral disorder and helps to maintain oral hygiene. Herbal mouthwash is suitable for any age group due to less or no other side effect instead of chemical mouthwash. Aqueous extract of Neem, Turmeric, Clove, Fennel, Guava leaves, Pudina, Ginger, Tulsishows effective antimicrobial, antifungal activity, anti-inflammatory and anti-plaque properties. In this research work herbal mouth wash was evaluated depends on various parameter like color, pH, Visual appearance, Phase separation, Homogeneity and antibacterial properties.

Plant‐based feed additives in Cyprinus carpio aquaculture

AbstractSpecies feeding lower at the food web like cyprinids (carp) are often advocated for sustainable aquaculture. However, their gastrointestinal biology (lack of stomach) has limited their acid‐peptic digestion, acid lysis of plants and invertebrate tissues, and phosphorus uptake. This review presents data from 118 articles on the use of plant‐based feed additives (PFAs) in Cyprinus carpio (common carp). Our review shows that most PFA research in carp is focused on improving feed and nutrient utilization efficiency along various levels (in vivo digestibility, metabolizability to in situ nutrient loading), while other half is focused on ensuring animal welfare standards (biomass, immunity, welfare). Several reviewed plant‐based additives however tend to do both. The PFAs include essential oils (EOs), plant‐part extracts (PPEs), medicinal plants (herbs) and spices. The effective inclusion rate of EOs is lower (0.5%–0.75%). Whereas PPEs, herbs and spices work at a generic inclusion rate of 2%–5% in carp feeds. Even at an optimum inclusion rate, there are factors controlling PFAs efficacy such as methods of preparation (reviewed). PFAs like lemon beebrush, geranium, dill, Rosemary pepper and common mallow with/without certain pre‐conditions are better to avoid in cyprinid diets. Although certain PFAs may potentially alter organoleptic qualities of carp flesh or might assist in training carp immunity, information on these aspects is limited. The review also provides a quick guide to researchers and feed companies interested in exploring the potential of PFAs in C. carpio aquaculture. Potential PFAs that could be valuable for cyprinids, but not yet explored are also listed.

Antimicrobial and Antioxidant Evaluation of Various Parts of Gossypium Herbacium (Linn) Plant

The methanol extracts of the various plant parts were screened for anti-oxidant activity by thin layer chromatography using 2,2-diphenyl-1-dipicrylhydrazyl (DPPH) while in vitro antimicrobial evaluation was determined by agar diffusion method and bioautographic technique using Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa as test organisms; aqueous solvent served as control and ciprofloxacin served as standard drug. The result showed that methanol extracts of the stem, leaf, root and seed of the plant were found to be stronger against the entire test organisms and the stem and leaf of the plant were also found to possess strong antioxidant activity. The antimicrobial property shown by the leaf is an evidence of the ethno medicinal uses of the plant. G. herbaceum leaf, seed, epicarp, stem bark, root bark, may provide novel plant-derived therapeutic agents, effective in treating infectious diseases arising from multiple drug-resistant bacteria and a target in the management of oxidative stress that could easily lead to aging.

Phytochemical Composition, Bioactive Compounds, and Antioxidant Properties of Different Parts of Andrographis macrobotrys Nees.

Andrographis macrobotrys Nees is an ethnomedicinal plant belonging to the family Acanthaceae, distributed in the moist deciduous and semi-evergreen forests of the southern Western Ghats of India. The objective of this research was to determine the phytochemical composition and bioactive chemical components using gas chromatography and mass spectrometry (GC-MS) and to check the antioxidant potential of the plant part extracts. A. macrobotrys roots, stems, and leaves were obtained from the species' natural habitat in the Western Ghats, India. The bioactive compounds were extracted using a Soxhlet extractor at 55-60 °C for 8 h in methanol. Identification analysis of A. macrobotrys bioactive compound was performed using GC-MS. Quantitative estimation of phytochemicals was carried out, and the antioxidant capacity of the plant extracts was determined by 2,2'-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) and ferric reducing assays (FRAP). A. macrobotrys has a higher concentration of phenolics in its stem extract than in its root or leaf extracts (124.28 mg and 73.01 mg, respectively), according to spectrophotometric measurements. GC-MS analysis revealed the presence of phytochemicals such as azulene, 2,4-di-tert-butylphenol, benzoic acid, 4-ethoxy-ethyl ester, eicosane, 3-heptadecanol, isopropyl myristate, hexadecanoic acid methyl ester, hexadecanoic acid, 1-butyl-cyclohexanol, 9,12-octadecadienoic acid, alpha-monostearin, and 5-hydroxy-7,8-dimethoxyflavone belonging to various classes of flavonoids, terpenoids, phenolics, fatty acids, and aromatic compounds. Significant bioactive phytochemicals include 2,4-di-tert-butylphenol, 2-methoxy-4-vinylphenol, 5-hydroxy-7,8-dimethoxyflavone, azulene, salvigenin, squalene, and tetrapentacontane. In addition, the antioxidant capability of each of the three extracts was assessed. The stem extract demonstrated impressive DPPH scavenging and ferric reduction activities, with EC50 values of 79 mg/mL and 0.537 ± 0.02 OD at 0.2 mg/mL, respectively. The results demonstrated the importance of A. macrobotrys as a source of medicine and antioxidants.

Effect of tomato (Solanum lycopersicum) extract on height and number of roots in patchouli (Pogostemon cablin Benth.) subculture planlet

Extracts of plant parts have the potential as growth regulators that can be used in in vitro culture. In order to find the ideal tomato extract concentration for the growth of patchouli explan, this study examine the effect of tomato extract (Solanum lycopersicum) as natural growth regulator on the height and quantity of roots in patchouli (Pogotemon cablin Benth.) subculture. This research was conducted from March to May 2022 at the Laboratory of Plant Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University. This study used a completely randomized design (CRD) with 6 treatments and 5 replications. The treatments consisted of P0 (MS medium 0), P1 (MS medium + 4 ml/L tomato fruit extract), P2 (MS medium + 6 ml/L tomato fruit extract), P3 (MS medium + 8 ml/L tomato fruit extract), P4 (MS medium + 10 ml/L tomato fruit extract) and P5 (MS medium + 12 ml/L tomato extract). Analysis of Variance (ANOVA) and continued by Duncan post-hoc test at a significant level of 95% applied to data by using SPSS version 18. The outcomes demonstrated that the injection of tomato fruit extract had a substantial impact on the roots rather than the height parameter plantlets. The control treatment (MS 0) resulted in the best average number of roots, it was suspected that the tomato fruit extract contained compounds which inhibited root growth.

In vitro nematocidal potential of hydro-ethanolic and aqueous extracts of Calotropis procera (Aiton) W.T. Aiton, 1811 (Apocynaceae) and Faidherbia albida (Delile) A. Chev., 1934 (Fabacae) against Onchocerca ochengi and Caenorhabditiselegans

Onchocerciasis caused by Onchocerca volvulus Leuckart, 1893 is the second-world infection responsible for human blindness. Except Ivermectin which has as targets the microfilariae of that parasite, specific treatment for this disease does not exist and in developing countries, medicinal plants seem to remedy that health problem. For that, aqueous and hydro-ethanolic leaf, bark, and root extracts of Calotropis procera and Faidherbia albida were evaluated in vitro, against the most popular bovine model, Onchocerca ochengi and the free-resistant nematode Caenorhabditis elegans. O. ochengi microfilariae and adults extracted from the bovine nodules and skins as well as the free strains of C. elegans were exposed to the various concentrations of the plant parts extracts and Ivermectin. In results, all the plant parts extracts were rich in tannins, saponins, alkaloids, flavonoids, phenols, coumarins, and glycosides. Phenols (175.45 ± 0.01 mg EGA/g DM), flavonoids (158.98 ± 0.05 mg EC/g DM), and tannins (89.98 ± 2.56 mg ETA/g DM) contents were high in the bark hydro-ethanolic extract of F. albida. The leaf hydro-ethanolic extract of F. albida induced high activity against O. ochengi microfilariae (CL50 = 0.13 mg/mL). The bark hydro-ethanolic extract of F. albida was also the most effective on O. ochengi adults and particularly on female adults (CL50 = 0.18 mg/mL). Against the parasite strain resistant to Ivermectin, F. albida leaf hydro-ethanolic extract appeared more active with CL50 = 0.13 mg/mL. Similarly, the bark hydro-ethanolic extract of F. albida was the most potent on the wild strain of C. elegans. Thus, this study validates the use of these plants by traditional healers in the management of onchocerciasis and suggests a new source of isolation of the potential plant compounds against Onchocerca.

Antimicrobial Activity of Leaf and Stem Extracts of Adansonia digitata

The species of Adansonia Digitata L. (Baobab) is an important multipurpose tree found to possess an enormous range of medicinal properties and can be used for the treatment of various diseases. Powdered leaves and stem of Adansonia digitata were extracted with 100% ethanol and hydro ethanol using the Soxhlet method of extraction. This research was conducted to determine the antimicrobial efficacy of Adansonia digitata stem and leaf extracts. The antimicrobial activity of ethanolic and hydroethanolic leaf, stem extracts of Adansonia digitata has been analyzed. The activity of the extracts of different plant parts against both bacteria and fungi strains were screened through disk diffusion assay along with the crude extract was carried out as control experiments. The antimicrobial activity of the leaf and stem extract of the plants showed that the plant extract used was effective against the different isolates tested. According to our observation, ethanolic extract of leave and stems showed higher antimicrobial activity.

Pharmacological activities of Artemisia absinthium and control of hepatic cancer by expression regulation of TGFβ1 and MYC genes.

Plant derived compounds have always been an important source of medicines and have received significant attention in recent years due to their diverse pharmacological properties. Millions of plant-based herbal or traditional medicines are used to cure various types of cancers especially due to activation of proliferative genes. The aim of the present study was to characterize the altered and attenuated gene expression of the selected growth factor namely Transforming growth factor Beta -1 (TGFβ1) and MYC in human hepatoma-derived (Huh7) liver cancer cell lines in response to extracts of Artemisia absinthium dissolved in selected organic solvents. Ethanolic, methanolic and acetone extract of different plant parts (leaf, stem and flowers) was used to access the antiproliferative activity by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay. Quantitative Real-Time RT-PCR revealed that the transcript levels of TGFβ1 are induced in the samples treated with methanolic extract of Artemisia absinthium. Furthermore, reduced expression levels of MYC gene was noticed in cancerous cells suggesting antiproliferative properties of the plant. This study further highlights the resistance profile of various microbes by antimicrobial susceptibility test with plant extracts. In addition, antidiabetic effect of Artemisia absinthium have also shown positive results. Our study elucidates the potentials of Artemisia absinthium as a medicinal plant, and highlights the differential expression of genes involved in its mitogenic and anti-proliferative activity with a brief account of its pharmacological action.