Abundance Of Plant-parasitic Nematodes Research Articles

Non‐target effects of herbicides on soil nematode assemblages

Herbicides are used extensively to control weeds. However, little is known about the non-target effects of herbicides on soil nematode assemblages. The objective of this study was to determine whether herbicides affect the abundance of nematodes in specific trophic groups. Meta-analysis was performed, and the calculated effect size, lr, quantified the impact of herbicides on the abundance of total nematodes and five trophic groups (bacterivores, fungivores, plant parasites, omnivores and predators). Measurements of lr indicated that herbicides decreased abundance of both fungivores and predators; however, abundance of bacterivores, plant parasites and omnivores increased. Overall, total nematode abundance tended to increase in response to herbicide application. The decrease in predator abundance suggests that herbicide application disturbs soil food webs. The increase in bacterivore and decrease in fungivore abundance suggest that bacterivores are more tolerant and both fungivores and predators more sensitive to herbicide applications. Herbicides also have non-target effects on omnivores, which may be due to the increased amount of food resources for omnivores after weed control. Additionally, the use of herbicides may result in a risk of an increase in plant-parasitic nematode abundance.

SRI-A Method for Sustainable Intensification of Rice Production with Enhanced Water Productivity

Climate change induced higher temperatures will increase crops’ water requirements. Every 10°C increase in mean temperature, results in 7% decline in the yield of rice crop. Hence, there is a need to develop water saving technologies in rice which consumes more than 50% of the total irrigation water in agriculture. System of Rice Intensification (SRI) is one such water saving rice production technology. Experiments were conducted at different locations in India including research farm of Directorate of Rice Research (DRR), Hyderabad, during 2005-10 to assess the potential of SRI in comparison to normal transplanting/Standard Planting (NTP/SP) under flooded condition. SRI recorded higher grain yield (6 to 65% over NTP) at majority of locations. Long term studies clearly indicated that grain yield was significantly higher (12-23% and 4-35% over NTP in Kharif and Rabi seasons, respectively) in SRI (with organic+ inorganic fertilizers) while the SRI (with100% organic manures), recorded higher yield (4-34%) over NTP only in the Rabi seasons. Even though, SRI resulted in higher productivity, the available nutrient status in soil was marginally higher (10, 42 and 13% over NTP for N, P and K, respectively) at the end of four seasons. There was a reduction in the incidence of pests in SRI and the relative abundance of plant parasitic nematodes was low in SRI as compared to the NTP. About 31% and 37% saving in irrigation water was observed during Kharif and Rabi seasons, respectively in both methods of SRI cultivation over NTP. SRI performed well and consistently reduced requirement of inputs such as seed and water in different soil conditions. SRI method, using less water for rice production can help in overcoming water shortage

Nematodes for Soil Quality Monitoring: Results from the RMQS BioDiv Programme

A French programme, “Reseau de mesure de la qualite des sols: biodiversite des organismes” (RMQS BioDiv) was developed in Brittany (27,000 km2 in the western part of France) as an initial assessment of soil biodiversity on a regional scale in relation to land use and pedoclimatic parameters. The nematode community assemblages were compared among the land use categories. Crops were characterised by a high abundance of bacterial-feeders, particularly opportunistic bacterial-feeders belonging to Rhabditidae. Meadows presented a higher total abundance of nematodes than did crops (20.6 ind·g-1 dry soil vs. 13.1 ind·g-1 dry soil), and they were mainly linked to the great abundance of plant-parasitic nematodes, particularly Meloidogyne, but with a very high heterogeneity between sampled plots. The nematodes were most abundant in forests (23.7 ind·g-1 dry soil) and presented the most structured community (SI = 82.2 in forests vs. 58.6 and 55.5 in crops and meadows, respectively). Forests had also the higher fungal component (fungal-feeders and facultative plant-feeders belonging to the Tylenchidae) leading to a significant higher part of the fungal decomposition pathway in forests than in crops. The ability of different taxonomic levels of nematode identifica- tion to discriminate among different cropping systems (i.e., continuous cropping system, crop with meadow in the rota- tion, meadow with crop in the rotation and permanent meadow) was also tested. The family level (48 families identified in these samples) was more efficient than the other taxonomic levels (86 taxa, 17 functional guilds and 6 trophic groups): best statistical significant discrimination for time spent in identification. The relation between the nematode ecological indices, the abundance of nematode trophic groups and the crop management practices were studied. The effects of fertilization, ploughing frequency, use of pesticides and management systems on ecological indices, particu- larly on the Maturity Indices, were observed.

Effects of yellow mustard amendments on the soil nematode community in a potato field with focus on Globodera rostochiensis

Green manures from the Brassicaceae family are used to control different soil-borne pests, including plant-parasitic nematodes due to their biofumigant compounds released during the breakdown of the plants. We studied the biofumigant and biological soil disinfestation effects of a yellow mustard green manure on the potato cyst nematode Globodera rostochiensis, a quarantine species with high economic importance for potato growers worldwide, in the field. In addition, we recorded the changes in the composition of the free-living nematode community for a better understanding of the overall ecological effect of this biocontrol method. We followed agricultural practices of sowing the green manure in late summer and incorporation in fall (with or without plastic cover) before planting of potato. Hatching and infectivity of G. rostochiensis juveniles were not consistently affected by growing yellow mustard or its amendments, but the population densities of plant-parasitic nematodes in general were reduced. Changes in the abundance of nematode trophic groups were observed for treatments with yellow mustard amendment. In addition, a beneficial effect was observed when covering the plots with plastic, as the abundance of plant-parasitic nematodes decreased. The amended plots exhibited an increase of the enrichment index while the channel index decreased significantly, indicating a soil food web with a decomposition pathway dominated by bacterial-feeder nematodes.

Mulch type affects soil biological functioning and crop yield of conservation agriculture systems in a long-term experiment in Madagascar

Conservation agriculture (CA) is rapidly developing in Madagascar but little is known about its effects on local soil functioning. To assess some of those effects, we investigated the effects of three CA systems and two levels of fertilization on soil functioning using nematofauna as indicator. The systems consisted in (i) soybean ( Glycine max L.)-maize ( Zea mays L.) rotation with mulch of residues, CA-R; (ii) bean ( Phaseolus vulgaris L.)-soybean rotation with living mulch of Pennisetum clandestinum, CA-K; (iii) continuous maize with living mulch of Desmodium uncinatum, CA-D and were compared with soybean–maize under conventional tillage (CT) and natural fallow (NF). The fertilization levels consisted in (i) farmyard manure, FYM; and (ii) farmyard manure + mineral fertilizers, FYM + NPK. Located in the Highlands of Madagascar, the experiment was setup in 1991 and andic Dystrustept soil had been sampled in 2005–2007. We measured nematode abundances and ecological indices as well as the abundance and biomass of soil macrofauna, soil water and organic C and N contents and plant yields. We hypothesized that (1) CA including maize in monoculture would lead to higher abundance of plant-parasitic nematodes; (2) both dead-residue mulch and inorganic fertilization would lead to a more basal nematode community structure; and (3) that the combination of system effects on soil nematode community would be able to forecast differential crop yields for the CA systems. Our results show that CA systems tested were able to support better/comparable maize and soybean yields compared with CT, provided that crop rotation is correctly managed. Supporting our first hypothesis, abundance of plant parasitic nematodes was (40–150 times) higher under maize monoculture. Abundance of soil nematofauna and trophic groups (excepted carnivores and omnivores) was stable during the three years. Inorganic fertilization increases carnivorous and omnivorous nematodes to 122% and 140%, respectively. Ecological indices showed that soil functioning of CA systems was intermediate between that NF and CT. CA systems were characterized by a highly structured soil food-web compared with CT. Yet, soil processes intensity revealed to be lower in CA with dead mulch compared with CA with living mulch, contrasting with our second hypothesis. The characterization of nematofauna discriminated well the different systems and supports our third hypothesis. Nematode structure and enrichment indices were significantly correlated to soil organic C and N content as well as grain yields. They proved to be powerful bio-indicators of soil functioning in the CA systems studied.

Effects of organic amendments on plant-parasitic nematode populations, root damage, and banana plant growth

Nematodes are major pests for crops, including banana. Environmentally friendly methods for managing plant-parasitic nematodes have to be developed, such as organic material application. Our study focuses on the impacts of several organic amendments on banana plants, considering mainly their effect on soil nitrogen supply and soil microbial biomass, and the consequences on plant-parasitic nematode impacts on the plants. A microcosm experiment for 13 weeks was conducted to evaluate four organic materials: sugarcane bagasse, sugarcane sludge, plant residues, and sewage sludge, compared to a control without organic amendment. Input of organic materials led to an important change on nitrogen resource, and plants grew better when the N availability was the highest, but better growth conditions did not necessarily reduce parasitic nematodes impacts on the roots. Damage on the roots depended on plant-parasitic nematode abundance. Three of four tested amendments exhibited a regulator effect on plant-parasitic nematode populations (bagasse, sugarcane sludge, and plant residues). Root growth was not the explanatory factor for this regulation. Only sugarcane sludge led to an overall positive effect on the plant, increasing its growth and reducing its parasitism pressure. The other organic materials exhibited an antagonism between the promoted plant growth and the reduced nematode populations.

Effects of organic amendment and tillage on soil microorganisms and microfauna

Soil microorganisms (bacteria, fungi) and microfauna (nematodes, protozoa) have been shown to be sensitive to organic amendments, but few experiments have investigated the responses of all these organisms simultaneously and across the soil profile. We investigated the impact of organic amendment and tillage on the soil food web at two depths in a field experiment. Over three growing seasons, field plots received seasonal organic amendment that was either incorporated into the soil (tilled) or not (no-till) as part of a tomato/soybean/corn cropping system. Un-amended, control plots that were either tilled or no-till were also included. We hypothesized that the addition of amendments would have a bottom–up effect on the soil food web, positively influencing the abundance of microorganisms, protozoa, and nematodes, primarily in the surface layers of the soil, but that this effect could be extended into deeper layers via tillage. Organic amendment had positive effects on most measured variables, including organic matter, respiration, protozoan and nematode density, and the abundance of PLFA biomarkers for bacteria and fungi. These effects were more pronounced in the 0–5 cm depth, but most variables increased with amendment in the deeper layer as well, especially with tillage. Denaturing Gradient Gel Electrophoresis (DGGE) of bacterial rDNA fragments indicated that distinct bacterial communities were selected for among tillage and amendment treatments and depths. Nematode faunal indices were not influenced by amendment, however. Increased nematode density in amended soils encompassed all trophic groups of free-living nematodes, with the greatest response among fungal-feeders, particularly with tillage. Increased biomass of microorganisms and decomposer microfauna in amended, tilled soils (0–5 cm depth) corresponded with a decline in the abundance of plant-parasitic nematodes. In control soils (0–5 cm depth), tillage reduced the relative abundance of fungal-feeding nematodes and increased the density of bacterial-feeding nematodes, in particular nematode species contributing to the Enrichment Index. When combined with organic amendment however, tillage was associated with increases in fungal-feeding nematodes and fungal biomarker PLFA. The results of this study suggest that when combined with amendment, tillage enhances the soil food web beyond the effect of amendment alone and is associated with declines in plant-parasitic nematodes.

MANIPULASI CAHAYA UNTUK MENURUNKAN KELIMPAHAN NEMATODA PARASIT TUMBUHAN PADA Pembibitan KOPI

Light manipulation to reduce the plant parasitic nematodes abundance on coffee nursery. Plant parasitic nematode problem on monoculture coffee can be caused not only by the suitable hosts but also by the environmental changes, such as soil water content and soil temperature. A semi-field experiment was conducted in Sumberjaya, West Lampung on May-September 2007 with the aim to study the effect of shading levels and fertilizer levels of coffee nursery on plant parasitic nematodes abundance. The results showed that the shading level treatment on coffee nursery significantly affected the abundance of plant parasitic nematodes but did not affect the free-living nematode abundance. The relationship between the plant parasitic nematode abundance with the shading level is polynomial with the highest nematode abundance (500 individual/300 ml of soil) was under 40% shading level. The increase in shading level indirectly affected the increase in plant parasitic nematode abundance through the increas in of soil water content. Inorganic N, P and K fertilizer at the recommended level for coffee nursery increased the abundance of plant parasitic nematodes from 216 to 282 individual/300 ml of soil. The interaction between the shading level and fertilizer did not significantly affect the plant parasitic nematode abundance.

Short-term nematode population dynamics as influenced by the quality of exogenous organic matter

AbstractThe food specificity of nematodes, their high number of species and high abundance in every habitat where decomposition takes place, indicate that the structure of the nematode assemblage has a high information content. Since nematodes respond quickly to changes in soil management and since the nematode fauna can be efficiently analysed, the structure of the nematode assemblage offers an interesting instrument to assess changes in soil conditions. We studied the effect of five organic amendments on the short-term nematode population dynamics and compared them to minerally fertilised and unfertilised plots. The experiment was started in 2005, and samples were taken in spring and autumn 2006 and spring 2007. In spring 2006, no clear differences among treatments in the diversity of free-living nematodes were observed, probably since the organic amendments were applied only twice, of which the last application was carried out 7 months before the sampling. At the second and third sampling, the enrichment index in the organically amended plots was higher than in the unamended plots, owing to the organic matter application. However, in plots amended with farmyard manure and cattle slurry the number of bacterivores increased significantly, while in the compost plots the fungivorous nematodes tended to be higher. This resulted in a low channel index for the manure and slurry plots, indicating a predominant bacterial decomposition pathway, and a higher channel index in the compost plots, suggesting a greater proportion of fungal decomposition. These assumptions on the decomposition of the applied organic matter were strongly supported by the composition of the soil microbial community, determined through PLFA analysis: in the compost plots the bacteria to fungi ratio was lower than in the manure and slurry plots. At all sampling occasions there was a remarkably lower abundance of plant-parasitic nematodes in plots amended with slurry and manure, indicating a negative impact of both amendments on plant-parasitic nematodes. We can conclude from this study that the fertiliser regimes affected the nematode assemblage, but that more samplings in the future are certainly necessary to assess adequately the impact of the different organic amendments.

Cover crops and organic mulches for nematode, weed and plant health management

AbstractTraditional cover cropping systems for nematode management seldom consider weed and soil nutrient management concurrently. Integrating cover crops suppressive to plant-parasitic nematodes with a cover crop mulching system could improve traditional approaches. Two field experiments were conducted in 2003 and 2004 to evaluate 'Tropic Sun' sunn hemp (Crotalaria juncea) and 'Iron Clay' cowpea (Vigna unguiculata) as summer cover crops and as organic mulches. Both experiments were in a 3 × 3 split-plot design in which the main plots were summer planting of sunn hemp, cowpea or fallow, and the subplots were organic mulch of sunn hemp, cowpea or no mulch. The summer cover crop was followed by turnip (Brassica rapa) and lima bean (Phaseolus lunatus) in the autumn. Using sunn hemp as organic mulch suppressed root-knot nematodes more effectively than using it as a cover crop, but only on a less susceptible host such as turnip, and not on a very susceptible host such as lima bean. While sunn hemp as a cover crop failed to enhance beneficial free-living nematodes, sunn hemp as an organic mulch enhanced bacterial-feeding nematode population densities. Sunn hemp mulch also suppressed broadleaf weeds but not grasses or nutsedges. Although sunn hemp and cowpea cover crops did not increase lima bean N and K content, their mulches increased N and K content. Similar results were observed for turnip and lima bean yields. Population density of root-knot nematodes was positively related to abundance of omnivorous nematode in 2003. The abundance of plant-parasitic nematodes was negatively related to the infestation levels of Pasteuria penetrans, and the abundance of predatory nematodes in 2004. Factors that might have affected the performance of sunn hemp on nematode communities are discussed.

Effects of Kodiak® (Bacillus subtilis Strain GBO3) on Soil-Inhabiting Nematodes Near the Rhizosphere of Treated versus Untreated Snap Bean Seeds In Situ

ABSTRACT A field study was conducted at the West Virginia University Organic Research Farm to determine the effect of the bacterial biological control agent Kodiak® on beneficial soil nematodes. Kodiak® was applied to bean seeds prior to planting, and predatory, bacterial-feeding, and plant parasitic nematode abundance near the bean roots was monitored during two growing seasons. The abundance of non-target, beneficial soil nematodes within and around bean rhizospheres did not differ overall among plants treated with Kodiak® versus untreated plants. The data presented here do not indicate any detrimental or non-target impacts of Kodiak® on soil nematodes. Additionally, bean yield was not affected when the product was used in a manner consistent with organic farming practices.

Plant parasitic and vector nematodes associated with Asiatic and Oriental hybrid lilies

A survey for distribution and abundance of plant parasitic nematodes in fields grown to Lilium in Himachal Pradesh, India at four study sites viz. Nagrota (at 810 m a.s.l.), Palampur (at 1270 m a.s.l.), Sunder Nagar (at 1400 m a.s.l.) and Chail (at 2250 m a.s.l.) was carried out. Moderate (101–500/200 ml soil) to high (501–1000/200 ml soil) populations of phytonematodes including the vectors for plant viruses ( Aphelenchoides avenae, Criconemoides spp., Hoplolaimus spp., Longidorus spp., Paratylenchus spp., Pratylenchus spp., Rhabditis spp., Trichodorus spp., Tylenchoryhnchus spp., Tylenchulus spp. and Xiphinema diversicaudatum) were recorded. Mean population of nematodes was positively correlated with pH in all the study sites, negatively correlated with electrical conductivity (EC), percent organic matter (OM%), available potassium (K) and positively correlated with percent carbon (C%), available nitrogen (N) and available phosphorus (P) in all but one study site. The highest incidence of virus-vector nematodes viz. X. diversicaudatum, Longidorus spp. and Trichodorus spp. was recorded at Palampur. Only Strawberry latent ringspot nepovirus (SLRSV) was detected in Lilium cvs. Star Gazer Max and Galeili by Enzyme Linked Immunosorbent Assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) and in X. diversicaudatum associated with the cultivars by RT-PCR. Cucumis sativus used as bait plants showed SLRSV symptoms after 15 days of nematode inoculation and further SLRSV was again detected by ELISA and RT-PCR in C. sativus plants confirming the transmission of SLRSV by X. diversicaudatum in Lilium.

Influence of some agroforestry practices on the temporal structures of nematodes in western Kenya

The influence of agroforestry practices on the temporal fluctuation of nematodes was studied in western Kenya. The experiment comprised a fallow phase, which had the following treatments: (1) maize/beans intercropping; (2) maize/beans intercropping with rock phosphate; (3) Crotalaria fallow; (4) Crotalaria fallow with rock phosphate; (5) Crotalaria fallow with rock phosphate (+ Calliandra and napier hedges); and a cultivation phase when all plots were planted to maize and beans. There were strong seasonal fluctuations in the abundance of both free-living and plant-parasitic nematodes. The abundance of plant-parasitic nematodes did not vary among cropping systems during the fallow phase, but varied significantly during the cultivation phase of the experiment. Pratylenchus spp. appeared to be stimulated by the application of phosphorus while Scutellonema spp. had higher populations in the maize crops, which were planted after a Crotalaria fallow. In both the fallow and the cultivation phases, the sampling date had a significant impact on nematode abundance. There were more plant-parasitic nematode species during the fallow phase of the study, but the evenness of the different nematode communities was significantly greater in the cultivation phase of the experiment.

Succession of soil nematodes in pine forests on coal-mining sands near Cottbus, Germany

The succession of soil nematodes from initial planting with Pinus sylvestris seedling to about 30-year-old pine plantations on coal mining sands in the Lusatian lignite-mining district near Cottbus (Germany) was studied and compared with the nematode fauna of a 40-year-old semi-natural pine forest on naturally formed sandy soil. The initial stage was primarily characterised by a very low abundance (20×10 3 individuals/m 2), which increased over a period of two years to values common in older pine plantations (500–600×10 3 individuals/m 2). In the semi-natural forest the mean abundance of nematodes was about 1300×10 3 individuals/m 2. Populations of Tardigrada, Rotifera and Enchytraeidae also increased with stand age. Nematode biomass increased from 49 to 543 mg m −2 in pine plantations and slightly decreased in the semi-natural forest to 301 mg m −2 over the period of investigation. The early colonisation of the initial stage was by bacterivorous ( Acrobeloides) and fungal feeding ( Aphelenchoides) nematodes, but the communities diversified as succession progressed with bacterivorous nematodes of the genera Plectus, Wilsonema and Metateratocephalus, root-fungal feeding Filenchus, omnivorous Aporcelaimellus and Eudorylaimus, and predacious Prionchulus becoming abundant. The abundance of plant-parasitic nematodes was very low. The greatest number of nematode genera was found in the semi-natural forest.

Establishment and yield of three ryegrasses following aldicarb use, and changes in abundance of plant parasitic nematodes

SUMMARYPure swards of Lolium multiflorum, L. multiflorum var. westerwoldicum and L. perenne sown in August had greater yields than autumn on areas treated with either 5 or 10 kg a.i./ha aldicarb than on untreated areas. Total annual dry matter yields increased by larger amounts where the greater rate of aldicarb was applied; throughout the trial, L. perenne was usually less affected by treatment than the other two grasses.Plant‐parasitic nematodes were more numerous on untreated than treated plots and were fewest in areas receiving the larger application (except in the second harvest year). Numbers of Tylenchorhynchus and Helicotylenchus were affected most by treatments; abundance of Paratylenchus and Criconemella were little altered.Stem‐boring Diptera invaded tillers of all three grasses but infection was least in L. perenne. Invasion occurred only on untreated plots in the establishment year and some yield benefit probably resulted from their control. In subsequent years invasion was similar on treated and untreated areas. Effects on other, non‐target organisms were not assessed.Ectoparasitic nematodes multiplied more in L. multiflorum plots than in others during the first full‐harvest year. The next year numbers of Paratylenchus only were greater in plots of L. perenne than in other plots.Sward persistence of L. multiflorum var. westerwoldicum was better on treated than untreated areas.It was concluded a) that aldicarb use greatly decreased nematode numbers and yields from treated areas were larger than from those untreated; b) that sward persistence was similarly improved following aldicarb use and c) that ryegrasses differed in their suitability as hosts to ectoparasitic nematodes.