Plant Oils Research Articles

Study on the interfacial properties of rice bran oil bodies affecting their physicochemical properties, stability and adsorption behavior at the oil-water interface for the design of precisely emulsified systems

Plant oil bodies (OBs) consist of a continuous monolayer of phospholipids-interfacial proteins covering the central triglycerides, which confers them extreme physicochemical stability. In this paper, rice bran OBs (RBOBs) extracted with NaHCO3 as a medium at pH 7.5–11.0 were naturally pre-emulsified spherical droplets with integral interfacial structures. The extraction pH determined the physicochemical properties, stability and interfacial behavior of RBOBs by affecting their interfacial properties. RBOBs extracted at pH 7.5 had more protein chains inserted into the acyl chains of the lipids, causing the RBOBs to be more stable, whereas at pH 11.0, the contents of PL and protein supported on the surface of TAGs core decreased significantly, the interfacial proteins were more flexible and looser, and part of the hydrophobic amino acids originally inserted in the TAGs core were exposed, destabilizing the OBs. RBOBs could spontaneously adsorb at the oil-water interface and form an interfacial film with elasticity as the primary feature. The structural rearrangement of the surface-active components was crucial to the formation of the interfacial membrane. More small-sized OBs like Pickering stabilizers adsorbed intact at the oil-water interface, creating ordered dense film (monolayer) particles. In contrast, large-sized RBOBs readily ruptured with a more pronounced degree of structural rearrangement of phospholipid-protein membrane fragments and disordered interfacial adsorption behavior. The results provide a reference for the development of precisely emulsified systems based on the special structure of plant OBs.

168 Bentley Lecture: Feeding Co-Products to Pigs to Reach Sustainable Food Production and Reduce Feed Cost

Abstract Changes occurred in the last two pandemic years that resulted in tremendous pressures on feedstuff supply. In Canada, causes for reduced cereal, pulse, and oilseed grain supply included a failed crop harvest in Western Canada in 2021 and increased demand for plant protein for food ingredients and for plant oils for cooking and renewable diesel added further pressure. Since January 2022, COVID-associated inflationary pressures, strained supply chains, the Russian invasion of Ukraine, and speculation have increased feedstuff prices further. Grains and tubers may serve as feedstuffs but are also processed into human food, fuel, and bio-industrial products. Together with these products, feed co-products such as distillers dried grains with solubles, canola meal and expeller, wheat millrun and bran, and sugar beet pulp are produced. As omnivores, pigs are ideally suited to convert these non-human edible co-products into high quality animal protein for human consumption. Thereby, co-products can reduce reliance on human edible grains to raise pigs and partially offset increases in feed cost provided their price is less per unit of net energy or digestible lysine, but also present risks, feeding challenges, and opportunities. First, processing of co-products adds variability in macronutrient profile beyond the intrinsic variability of crops. Thus, feed quality evaluation to regularly update digestibility profiles of energy, amino acid, and phosphorus is important. Second, fermentation and heat processing impact amino acid and phosphorus availability. Overheating reduces lysine availability due to Maillard reactions, reduces heat-labile anti-nutritional factors, but combined with fermentation, may increase mineral availability. Third, co-products may possess starch and fiber characteristics that benefit gut health. Fourth, co-products may contain chemical residues and mycotoxins such as deoxynivalenol that survive or are augmented by processing that reduce voluntary feed intake. Fifth, due to their decreased digestibility, dietary inclusion of co-products generally increases excretion of organic matter, P, and N by pigs. Technologies such as tail-end processing and feed enzymes are thus required to increase nutrient digestibility. Finally, dietary inclusion of co-product may impact carcass characteristics and pork quality. For example, inclusion of high fiber co-products reduces dressing percentage and inclusion of high unsaturated fatty acid co-products softens pork fat. In conclusion, the feeding of co-products may reduce feed costs per unit of pork produced, but also provides challenges to achieve cost-effective, predictable growth performance, carcass characteristics, and pork quality. While feeding co-products remains a solution to reduce feed cost, feeding co-products in substitution of grains is now also seen as a piece of the puzzle to reach sustainable food production systems.

LC-Orbitrap-HRMS Determination of Two Novel Plastochromanol Homologues.

The antioxidant plastochromanol-8 (PC-8) is a tocochromanol which differs from γ-tocotrienol in having an unsaturated side chain of eight instead of three isoprene units. The recent isolation of PC-8 from flaxseed oil indicates the additional presence of lower shares of two previously unknown homologues, plastochromanol-7 (PC-7) and plastochromanol-9 (PC-9), which feature seven and nine isoprenoid units respectively on the γ-chromanol backbone. Here, a fast LC-Orbitrap-HRMS method is applied for the determination of PC-7 and PC-9 in seven plant oils and a plant extract. The presence of PC-7, PC-8, and PC-9 is confirmed in all eight investigated samples by LC-Orbitrap-HRMS analysis after saponification. PC-8 amounts of ≈315-350 mgkg-1 in two flaxseed oils, ≈75 mgkg-1 in rapeseed oil, ≈38mgkg-1 in camelina oil, ≈80-120mgkg-1 in two mustard oils, ≈90 mgkg-1 in candle nut oil, and ≈900 mgkg-1 dry weight in Cecropia leaves are determined by quantification. Semi-quantification of PC-7 and PC-9 indicated the presence of ≈0.1-1% of PC-7 and PC-9 in varied relative ratios. The novel plastochromanol homologues are of particular interest to researchers with focus on vitamin E and other tocochromanols because of their unexplored bioactivity.

Alternative locust control with a dilutable linseed oil emulsion

AbstractLocust swarm control is still a main topic in agriculture and is often based on the extensive use of chemical agents that can be problematic for the environment and human health. Currently, only a few alternative control agents are available on the market, such as neem oil, the fungus Metarhizium sp., and the microsporidian Paranosema locustae. In this study, we improved the formulation of a botanical insecticide that is based on linseed oil and was found to be effective against the desert locust, Schistocerca gregaria. To make sure that linseed oil is most effective, we first tested several natural plant oils in a comparative laboratory study. Linseed oil performed best and resulted in 100% mortality within only 2 days after a single spray treatment. However, this linseed oil formulation contains three expensive essential oils, thus we further improved it to avoid costly essential oils. A much lower concentration of linseed oil was also found to be highly effective and killed adult S. gregaria within 3 days in an experiment performed under semifield conditions. Furthermore, a concentrated stock solution of this botanical insecticide was developed to increase stability and reduce shipping costs in areas where water for dilution is available. Mechanical treatment, applied during the preparation of this linseed oil emulsion, resulted in the formation of micro‐colloids, which prevent the separation of phases and enable 1:16 dilution with water containing bicarbonate. Interestingly, only 24 h after single spray treatment, locusts became immobile and most of them were unable to jump, which would make them easy prey for natural enemies. Later, insects died due to the hardening of the linseed oil emulsion. This novel linseed oil emulsion is harmless for humans, rather easy to produce, and remains stable over weeks in its concentrated form, which suggests that it is promising alternative agent for locust control.

PROSPECTS OF GROWING MEDICINAL, ESSENTIAL OIL AND SPICY AROMATIC CULTURES

This work is devoted to the analysis of the most common issues faced by manufacturers of medicinal and essential oil raw materials — the market, the availability and availability of wild raw materials, agro-ecological conditions, the amount of manual labor, its cost, investments in quality seeds and equipment, post-harvest processing and the overall profitability of production. Cultivation of medicinal, essential oil and aromatic plants today is not only a promising alternative to the collection of wild raw materials, which allows preserving the natural biodiversity of plants with medicinal properties, including those species that have a protective status, but is also a powerful branch of the economy that provides high quality raw materials for the pharmaceutical, cosmetic and food industries. As a rule, medicinal and essential oil plants are considered by agricultural producers as secondary crops, as their cultivation is associated with certain problems. These problems are common to both small and powerful agricultural enterprises, both in Ukraine and abroad. They are related to both technological aspects of growing and plant identification, as well as market fluctuations and product prices. Consumers of medicinal and essential oil raw materials increasingly prefer cultivated products, because under such conditions it is easier to predict yield and ensure quality compared to wild-grown raw materials. During cultivation, false identification and unintentional falsification of raw materials are almost excluded, and the use of improved methods of cultivation and the use of modern varieties allows planning the harvest and costs. The profitability of growing medicinal and essential oil crops competes with traditional field crops, which have highly specialized equipment, a significant list of zoned varieties and hybrids, standard procedures for applying fertilizers and protective means, etc. In contrast to the cultivation of traditional crops, in order to ensure the profitability of the production of medicinal and essential oil raw materials, it is necessary to use methods with low production costs in order to be competitive on the international market and with the production of raw materials from natural groups. The work uses generalized material, both from our own many years of scientific and practical experience in the cultivation of medicinal and essential oil raw materials, as well as the experience of scientists and practitioners of foreign countries. On the example of the experience of foreign countries and domestic researchers and producers, the authors sought to prove that the cultivation of agricultural crops for obtaining essential oil and medicinal raw materials is a profitable business and can be considered as a variant of niche crops, be an attractive option for small farms and family farms. Within the scope of this review, it is impossible to consider all the problematic issues of the widespread introduction of medicinal and essential oil crops into the practice of agricultural production, since many more problems need to be solved, which can be the subject of future experiments and research.

Integrating Fermentation Engineering and Organopalladium Chemocatalysis for the Production of Squalene from Biomass-Derived Carbohydrates as the Starting Material

The transition from fossil resources to renewable biomass for the production of valuable chemicals and biobased fuels is a crucial step towards carbon neutrality. Squalene, a valuable chemical extensively used in the energy, healthcare, and pharmaceutical fields, has traditionally been isolated from the liver oils of deep-sea sharks and plant seed oils. In this study, a biochemical synergistic conversion strategy was designed and realized to convert glucose to squalene by combining fermentation technology in yeast with reductive coupling treatment of dienes. First, glucose derived from hydrolysis of cellulose was used as a renewable resource, using genetically engineered Saccharomyces cerevisiae as the initial biocatalyst to produce β-farnesene with a titer of 27.6 g/L in a 2.5 L bioreactor. Subsequently, intermediate β-farnesene was successfully converted to squalene through the organopalladium-catalyzed reductive coupling reaction involving the formation of Pd(0)L2 species. Under mild reaction conditions, impressive β-farnesene conversion (99%) and squalene selectivity (100%) were achieved over the Pd(acac)2 catalyst at a temperature of 75 °C in an ethanol solvent after 5 h. This advancement may provide insights into broadening squalene production channels and accessing the complex skeletons of natural terpenoids from biorenewable carbon sources, offering practical significance and economic benefits.

Comparative Study of Chemical Composition and Cholinesterase Inhibition Potential of Essential Oils Isolated from Artemisia Plants from Croatia

The essential oil (EO) of Artemisia plants contains a large number of bioactive compounds that are widely used. The aim of this study was to analyse the chemical composition of EOs of six Artemisia plants collected in Croatia and to test their cholinesterase inhibitory potential. GC–MS analysis of the EO of A. absinthium showed that the dominant compounds are cis-sabinyl acetate and cis-epoxy-ocimene; in EO of A. abrotanum, it is borneol; in the EO of A. annua, they are artemisia ketone, camphor and 1,8-cineole; in the EO of A. arborescens, they are camphor and chamazulene; in the EO of A. verlotiorum, they are cis-thujone, 1,8-cineole and trans-thujone; and in the EO of A. vulgaris, they are trans-thujone and trans-epoxy-ocimene. The EO of the five studied Artemisia species from Croatia is rich in monoterpenoid compounds (1,8-cineole, artemisia ketone, cis-thujone, trans-thujone, cis-epoxy-ocimene, camphor, borneol and cis-sabinyl acetate). The EO of A. arborescens is also rich in chamazulene. The results also showed that the tested EOs have moderate cholinesterase inhibition potential, especially the EOs of A. annua, A. vulgaris and A. abrotanum. This is the first analysis of the chemical composition of the EOs of four Artemisia plants and the first analysis of cholinesterase potential for plants collected in Croatia.

Which Moisturizer to Use in Scar Therapy after Burn Injuries? A Subjective and Objective Skin and Scar Evaluation after Topical Treatment with Dexpanthenol, Aloe Vera, and Plant Oil.

Background and Objectives: Good scar management in burn care is essential. Nevertheless, there are no consistent recommendations regarding moisturizers for scar management. Our aim was to investigate and compare the effects of commonly used products on normal skin and burn scars. Materials and Methods: A total of 30 skin-healthy (control group) and 12 patients with burn scars were included in this study. For an intraindividual comparison, each participant received creams consisting of dexpanthenol (P), aloe vera (A), and a natural plant oil (O) with instructions to apply them daily to a previously defined area for at least 28 days. Objective scar evaluation was performed with Visioscan®; Tewameter®; Cutometer®, and the Oxygen To See® device. Subjective evaluation was performed with an "application" questionnaire, the Patient and Observer Scar Assessment Scale (POSAS), and with the "best of three" questionnaire. Results: After (A) a high trend of amelioration of +30%, TEWL was detected on the scar area. Blood flow increased slightly on healthy skin areas after (A) application to +104%. The application of (A) on healthy skin demonstrated a positive effect on the parameters of scaliness (+22%, p < 0.001), softness (+14%, p = 0.046), roughness R1 (+16%, p < 0.001) and R2 (+17%, p = 0.000), volume (+22%, p < 0.001), and surface area (+7%, p < 0.001) within the control group. After (P), a significant improvement of the baseline firmness parameter of +14.7% was detected (p = 0.007). (P) also showed a beneficial effect on the parameters of R1 (+7%, p = 0.003), R2 (+6%, p = 0.001), and volume (+17%, p = 0.001). (O) lead to a statistically significant improvement of volume (+15%, p = 0.009). Overall, most study participants stated (A) to be the "best of three". Conclusions: (A) performed statistically best, and is a well-tolerated moisturizing product. However, further quantitative studies are needed to provide statistically significant clarification for uniform recommendations for scar therapy.

Production of capsaicinoid nonivamide from plant oil and vanillylamine via whole-cell biotransformation

Capsaicinoids are mostly derived from chili peppers and have widespread applications in food, feed, and pharmacology. Compared with plant extraction, the use of microbial cell factories for capsaicinoids production is considered as a more efficient approach. Here, the biotransformation of renewable plant oil and vanillylamine into capsaicinoid nonivamide was investigated. Nonivamide biosynthesis using nonanoic acid and vanillylamine as substrates was achieved in Escherichia coli by heterologous expression of genes encoding amide-forming N-acyltransferase and CoA-ligase. Through increasing nonanoic acid tolerance of chassis cell, screening key enzymes involved in nonivamide biosynthesis and optimizing biotransformation conditions, the nonivamide titer reached 0.5 g/L. By further integrating a route for conversion of oleic acid to nonanoic acid, nonivamide biosynthesis was finally achieved using olive oil and vanillylamine as substrates, yielding a titer of approximately 10.7 mg/L. Results from this study provide valuable information for constructing highly efficient cell factories for the production of capsaicinoid compounds.

Woodfordia fruticosa (L. Kurz): An Evidence-based Study of Its Phytochemical and Pharmacological Activity

Abstract: Woodfordia fruticosa (L.) Kurz, a member of the 'Lythraceae' family, has been used in traditional medicine to cure common diseases since ancient times. It is traditionally used to treat a variety of maladies such as the common cold, toothache, blood infection, leprosy, dysentery, wounds, rheumatic pain, fever, urinary issues, inflammation, antifertility, and menstrual problems. The huge potential and impacts of this plant have been thoroughly confirmed in in vitro and in vivo studies. The most current research on the plant Woodfordia fruticosa is critical for identifying the bioactive components responsible for its medicinal efficacy. Phytochemical studies reveal the existence of several chemicals extracted from various components of the plant, including glycosides, terpenes, flavonoids, tannins, sterols, phenolics, and essential oils. According to pharmacological studies, the plant has many medicinal properties, such as hypoglycemic, antioxidant, analgesic, hepatoprotective, antibacterial, gastric protective, and wound healing. Most of the pharmacological effects of raw extracts of this plant have been described. Only a few researchers have reported the activity of chemicals isolated from this plant. Therefore, this research will help to discover the many different medicinal properties of the plant as well as its pharmacological effects on various diseases and will show that further research is needed to explore its practical applications.

Tea Tree Oil Improves Energy Metabolism, Non-Specific Immunity, and Microbiota Diversity via the Intestine-Hepatopancreas Axis in Macrobrachium rosenbergii under Low Fish Meal Diet Administration.

Tea tree oil (TTO) is an essential plant oil with diverse antibacterial and antioxidant properties; however, whether the role played by TTO in low fish meal (LF) diets induced the observed effects in the farmed crustaceans remains unclear. Therefore, this study used Macrobrachium rosenbergii as the model crustacean, and an 8-week feeding experiment with NF (normal fish meal), LF (soybean meal replacing 40% fish meal), and LFT (LF with 200 mg/kg TTO) diets was conducted to evaluate the positive effects of TTO under the LF diet. Compared to the NF diet, the LF diet reduced hemolymph antioxidant capacity and non-specific immunity, and induced hepatopancreas apoptosis and damage. However, in comparison with LF, LTF significantly ameliorated morphological impairment in the hepatopancreas, improved hepatopancreas energy metabolism by upregulating the Bcl-2/Bax and Akt/mTOR pathways, and enhanced antioxidant and non-specific immune capacity by activating the NF-κB/NO pathway. In addition, LFT repaired intestinal barrier injury and the imbalance of intestinal microbiota induced by the LF diet. Moreover, the Pearson correlation revealed the variations of the above indicators, which were related to the abundance changes of Klebsiella, Clostridium sensu stricto 12, Thermobifida, Bifidobacterium, and Alistipes, indicating that these microbes might serve as prospective targets for the intestine-hepatopancreas axis to affect hepatopancreas apoptosis, metabolism, and non-specific immunity. In summary, 200 mg/kg TTO supplementation mediated gut microbiota and positively improved energy metabolism and non-specific immunity, thereby alleviating hepatopancreas dysplasia and damage induced by the LF diet in M. rosenbergii.

Improvement of the Maintenance Management system in a Petrochemical Complex and Reducing the Costs

This research is a survey and analysis which has been carried on and a variety of data on the maintenance programs such as the comprehensive overhaul of productive units (turnarounds), periodic maintenance and emergency corrective maintenance at an oil and gas processing plant. The information in this project combined results and data to improve the maintenance management system in an oil and gas processing plant and to achieve the goal of reducing expenses and therefore increasing profits. The data collected, and the results of plant survey were, then, thoroughly analysed to reach important recommendations applicable to most petrochemical facilities.

Screening of the action mechanisms involved in the antinociceptive effect of isopulegol and its complex in cyclodextrin using acute nociception models in mice

Isopulegol, a terpene compound commonly present in the essential oils of several aromatic plants, may present a number of important pharmacological activities. Besides the screening of the antinociceptive effect of the monoterpene isopulegol (ISO) and its inclusion complex in β-cyclodextrins (ISO/CD), this study also aimed at investigating the signaling pathways involved in the antinociceptive response. The effects of ISO and ISO/CD on the central nervous system (CNS) were evaluated through open-field and rota-rod assays and the antinociceptive effects in formalin models, abdominal contortions by acetic acid, hot-plate and plantar mechanical hypernociception. Male and female mice (Mus musculus), weighing between 20–30 g, were divided into 6-animal groups. The oral administration of isopulegol and its complex showed significant antinociceptive effects in the 10, 5 and 1 mg/kg doses for both the formalin and abdominal contortion assays, with highlights on the 10 mg/kg dose of ISO and ISO/CD in both assays. Likewise, the 10 mg/kg dose was selected for the evaluation of the effects on the CNS, central and peripheral antinociceptive effect and screening of the signaling pathways involved in the analgesic potential. ISO and ISO/CD, both in the 10 mg/kg dose v.o., did not show any alteration to the parameters evaluated of the CNS and the results pointed to a possible acute antinociceptive action in the tests of formalin, abdominal contortion, hot-plate and plantar mechanical hypernociception, suggesting the involvement of these signaling pathways: opioid, potassium channel, cholinergic, nitric oxide, cyclic guanosin monophosphate, vaniloid and glutamatergic. The results suggest that ISO and ISO/CD carry a promising antinociceptive potential as a possible alternative to the pharmacological treatment of pain, showing that the inclusion of ISO in cyclodextrins enhance its pharmacological properties, once that the complexation process involves much lower amounts of the compound – what contributes to a better bioavailability and weaker probability for the development of undesired effects.

Essential oils of Pinus sylvestris, Citrus limon and Origanum vulgare exhibit high bactericidal and anti-biofilm activities against Neisseria gonorrhoeae and Streptococcus suis

Antimicrobial resistance is a worldwide problem that urges novel alternatives to treat infections. In attempts to find novel molecules, we assess the antimicrobial potential of seven essential oils (EO) of different plants (Pinus sylvestris, Citrus limon, Origanum vulgare, Cymbopogon martini, Cinnamomum cassia, Melaleuca alternifolia and Eucalyptus globulus) against two multidrug-resistant bacteria species, i.e. Neisseria gonorrhoeae and Streptococcus suis. EOs of P. sylvestris and C. limon revealed higher bactericidal activity (MIC ≤ 0.5 mg/mL) and capacity to rapidly disperse biofilms of several N. gonorrhoeae clinical isolates than other EOs. Examination of biofilms exposed to both EO by electron microscopy revealed a reduction of bacterial aggregates, high production of extracellular vesicles, and alteration of cell integrity. This activity was dose-dependent and was enhanced in DNase I-treated biofilms. Antibiotic susceptibility studies confirmed that both EOs affected the outer membrane permeability, and analysis of EO- susceptibility of an LPS-deficient mutant suggested that both EO target the LPS bilayer. Further analysis revealed that α- and β-pinene and d-limonene, components of both EO, contribute to such activity. EO of C. martini, C. cassia, and O. vulgare exhibited promising antimicrobial activity (MIC ≤ 0.5 mg/mL) against S. suis, but only EO of O. vulgare exhibited a high biofilm dispersal activity, which was also confirmed by electron microscopy studies. To conclude, the EO of P. sylvestris, C. limon and O. vulgare studied in this work exhibit bactericidal and anti-biofilm activities against gonococcus and streptococcus, respectively.

Innovations in applications and prospects of non-isocyanate polyurethane bioplastics.

Currently, conventional plastics are necessary for a variety of aspects of modern daily life, including applications in the fields of healthcare, technology, and construction. However, they could also contain potentially hazardous compounds like isocyanates, whose degradation has a negative impact on both the environment and human health. Therefore, researchers are exploring alternatives to plastic which is sustainable and environmentally friendly without compromising its mechanical and physical features. This review study highlights the production of highly eco-friendly bioplastic as an efficient alternative to non-biodegradable conventional plastic. Bioplastics are produced from various renewable biomass sources such as plant debris, fatty acids, and oils. Poly-addition of di-isocyanates and polyols is a technique employed over decades to produce polyurethanes (PUs) bioplastics from renewable biomass feedstock. The toxicity of isocyanates is a major concern with the above-mentioned approach. Novel green synthetic approaches for polyurethanes without using isocyanates have been attracting greater interest in recent years to overcome the toxicity of isocyanate-containing raw materials. The polyaddition of cyclic carbonates (CCs) and polyfunctional amines appears to be the most promising method to obtain non-isocyanate polyurethanes (NIPUs). This method results in the creation of polymeric materials with distinctive and adaptable features with the elimination of harmful compounds. Consequently, non-isocyanate polyurethanes represent a new class of green polymeric materials. In this review study, we have discussed the possibility of creating novel NIPUs from renewable feedstocks in the context of the growing demand for efficient and ecologically friendly plastic products.

Community structure and niche differentiation of endosphere bacterial microbiome in Camellia oleifera.

Understanding the changes in bacterial community structure in different microenvironments of Camellia oleifera is essential to better explore the benign interaction between beneficial microorganisms and plants. Using Camellia oleifera trees, a Chinese wooden oil plant as a model ecosystem, we characterized the archaeal and bacterial microbiome across five different tissue-level niches using 16S rRNA gene analyses. Our research indicates that the diversity of Camellia oleifera endophytic bacterial communities is highly dependent on the plant compartment. The species replacement process (69.90%) is the dominant factor in the differences in bacterial community structure. The dominant bacteria phyla (Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Chloroflexi, and Verrucomicrobia) of Camellia oleifera show a significant plant compartment (roots, stems, leaves, fruits) enrichment effects. A variety of bacteria (Hymenobacter, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Mesorhizobium, Bradyrhizobium, Bacillus, Ochrobactrum, Pantoea, Pseudomonas, etc.) with nitrogen-fixed potentials are enriched in Camellia oleifera tissue. In addition, the hub bacterial groups of Camellia oleifera are Nitrospira, Haemophilus, Staphylococcus, Ruminiclostridium, and Ochrobactrum. They are widespread colonization in various tissues with a low relative abundance and may play an important role in the nitrogen cycle, host life promotion, and plant defense. This study provides a holistic understanding of the endosphere bacterial community structure, which is one of the most complete ecological niche-level analyses of Camellia oleifera. These results provide a scientific theoretical basis for an in-depth discussion of plant-endosphere microbial interaction and better exploration of benign interaction of beneficial microorganisms and plants.IMPORTANCEMicroorganisms inhabited various tissues of plants and play a key role in promoting plant growth, nutritional absorption, and resistance. Our research indicates that the diversity of Camellia oleifera endophytic bacterial communities is highly dependent on the plant compartment. Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Chloroflexi, and Verrucomicrobia are dominant bacteria phyla. The tissues of Camellia oleifera contain various bacteria with nitrogen fixation potential, host life promotion, and plant defense. This study provides a scientific theoretical basis for an in-depth discussion of plant-endosphere microbial interaction and better exploration of benign interaction of beneficial microorganisms and plants.

Evaluation of the efficiency of some vegetable oils and bio-fungi in controlling Aphis fabae Scopoli of black bean insect.

The experiment was conducted to evaluate the efficiency of some vegetable oils such as watercress oil, aloe vera oil and eucalyptus oil in the percentage of nymphs of black broad bean Aphis fabae Scopoli on the chard plant Beta vulgaris subbsp. cicla. The mortality rate was 83.88, 90.56, and 93.89 % for oils used in the laboratory, while the percentage of loss in the field was 82.91. 88.19 and 89.86%, respectively, the results showed that vegetable oils had an effect on the destruction of whole black broad bean insects, and the increase of this effect with the increase of the time and concentration factor. Keywords: Aphis fabae; Plant oils; Biological fungi

Chemical, Thermal, and Mechanical Properties of Sulfur Polymer Composites Comprising Low-Value Fats and Pozzolan Additives

High sulfur-content materials (HSMs) formed via inverse vulcanization of elemental sulfur with animal fats and/or plant oils can exhibit remarkable mechanical strength and chemical resistance, sometimes superior to commercial building products. Adding pozzolan fine materials—fly ash (FA), silica fume (SF), ground granulated blast furnace slag (GGBFS), or metakaolin (MK)—can further improve HSM mechanical properties and stability. Herein, we detail nine materials comprised of rancidified chicken fat, elemental sulfur, and canola or sunflower oil (to yield CFS or GFS, respectively) and, with or without FA, SF, GGBFS, or MK. The base HSMs, CFS90 or GFS90, contained 90 wt% sulfur, 5 wt% chicken fat, and 5 wt% canola or sunflower oil, respectively. For each HSM/fine combination, the resulting material was prepared using a 95:5 mass input ratio of HSM/fine. No material exhibited water uptake &gt;0.2 wt% after immersion in water for 24 h, significantly lower than the 28 wt% observed with ordinary Portland cement (OPC). Impressively, CFS90, GFS90, and all HSM/fine combinations exhibited compressive strength values 15% to 55% greater than OPC. After immersion in 0.5 M H2SO4, CFS90, GFS90, and its derivatives retained 90% to 171% of the initial strength of OPC, whereas OPC disintegrated under these conditions. CFS90, GFS90, and its derivatives collectively show promise as sustainable materials and materials with superior performance versus concrete.

Unraveling the evolutionary dynamics of the TPS gene family in land plants.

Terpenes and terpenoids are key natural compounds for plant defense, development, and composition of plant oil. The synthesis and accumulation of a myriad of volatile terpenoid compounds in these plants may dramatically alter the quality and flavor of the oils, which provide great commercial utilization value for oil-producing plants. Terpene synthases (TPSs) are important enzymes responsible for terpenic diversity. Investigating the differentiation of the TPS gene family could provide valuable theoretical support for the genetic improvement of oil-producing plants. While the origin and function of TPS genes have been extensively studied, the exact origin of the initial gene fusion event - it occurred in plants or microbes - remains uncertain. Furthermore, a comprehensive exploration of the TPS gene differentiation is still pending. Here, phylogenetic analysis revealed that the fusion of the TPS gene likely occurred in the ancestor of land plants, following the acquisition of individual C- and N- terminal domains. Potential mutual transfer of TPS genes was observed among microbes and plants. Gene synteny analysis disclosed a differential divergence pattern between TPS-c and TPS-e/f subfamilies involved in primary metabolism and those (TPS-a/b/d/g/h subfamilies) crucial for secondary metabolites. Biosynthetic gene clusters (BGCs) analysis suggested a correlation between lineage divergence and potential natural selection in structuring terpene diversities. This study provides fresh perspectives on the origin and evolution of the TPS gene family.

Effectiveness of Using Electric Knapsack Sprayer in Weed Control on Oil Palm Plants (Elaeis guineensis Jacq)

Absract This study aims to determine the effectiveness of the use of an electric knapsack sprayer in controlling weeds in oil palm plants. This study was carried out in Sirapit District, Langkat Regency from April to June 2022. The data collection methods used were questionnaires/questionnaires and interviews. The analytical method used is a Likert scale to determine the level of effectiveness and multiple linear regression to determine the factors that affect the effectiveness of using an electric knapsack sprayer in controlling weeds on palm oil plants in Sirapit District, Langkat Regency. For the F test, Fcount is 8.463 &gt; Ftable 2.18, which means that simultaneously the variables of age, education, the role of extension agents, experience, land conditions, expertise, and operational costs have a significant effect on the effectiveness of using electric knapsack sprayers in weed control. on oil palm plantations. For the partial t-Test, the factors that significantly affect the effectiveness of using electric knapsack sprayers in controlling weeds in oil palm consist of the role of extension workers (t=3.424), land conditions (t=4.734), expertise (t=2.550), and operational costs (t=3.556), but it is not significantly for three factors, such as age (t=-1.302), education (t=0.854), and experience (t=-0.574). The results of this study showed that the effectiveness of using an electric knapsack sprayer in controlling weeds in oil palm was high (80.27%). Factors that influence the effectiveness of using electric knapsack sprayers in controlling weeds in oil palm plantations are the role of extension workers, land conditions, expertise, and operational costs. Keywords: Effectiveness, electric knapsack sprayer, palm oil, herbicide, weed