Plant Growth Test Research Articles

Hydroxamate siderophores produced by Streptomyces acidiscabies E13 bind nickel and promote growth in cowpea (Vigna unguiculata L.) under nickel stress

The siderophore-producing ability of nickel-resistant Streptomyces acidiscabies E13 and the role of the elicited siderophores in promoting plant growth under iron and nickel stress are described. Siderophore assays indicated that S. acidiscabies E13 can produce siderophores. Electrospray ionization mass spectrometry (ESI-MS) revealed that the bacterium simultaneously produces 3 different hydroxamate siderophores. ESI-MS showed that in addition to iron, all 3 siderophores can bind nickel. In vitro plant growth tests were conducted with cowpea (Vigna unguiculata) in the presence and absence of the elicited siderophores. Culture filtrates containing hydroxamate siderophores significantly increased cowpea height and biomass, irrespective of the iron status of the plants, under nickel stress. The presence of reduced iron was found to be high in siderophore-containing treatments in the presence of nickel. Measurements of iron and nickel contents of cowpea roots and shoots indicated that the siderophore-mediated plant growth promotion reported here involves the simultaneous inhibition of nickel uptake and solubilization and supply of iron to plants. We conclude that hydroxamate siderophores contained in culture filtrates of S. acidiscabies E13 promoted cowpea growth under nickel contamination by binding iron and nickel, thus playing a dual role of sourcing iron for plant use and protecting against nickel toxicity.

Evaluation of tests to assess the quality of mine-contaminated soils

An acid metal-contaminated soil from the Aljustrel mining area (a pyrite mine located in SW Portugal in the Iberian Pyrite Belt) was subjected to chemical characterisation and total metal quantification (Cd, Cr, Cu, Ni, Pb and Zn). Water-soluble metals were determined and a sequential extraction procedure was used to investigate metal speciation. Two bioavailable metal fractions were determined: a mobile fraction and a mobilisable fraction. Soil ecotoxicity was studied using a battery of bioassays: plant growth test and seed germination with cress (Lepidium sativum L.), earthworm (Eisenia fetida) mortality, E. fetida avoidance behaviour, luminescent inhibition of Vibrio fischeri and Daphnia magna immobilisation. Although the total content of Cu, Zn and Pb in the soil was large (362, 245 and 1,250 mg/kg dry matter, respectively), these metals were mostly structurally bound (87% for Cu, 81% for Zn and 89% for Pb) and, therefore, scarcely bioavailable. Nonetheless, the D. magna immobilization test using soil leachate showed an EC50 (48 h) of 36.3% (v/v), and the luminescent inhibition of V. fischeri presented an EC20 (15 min) of 45.2% and an EC20 (30 min) of 10.7% (v/v), suggesting a considerable toxic effect. In the direct exposure bioassays, E. fetida avoided the mine soil at the highest concentrations (50%, 75% and 100% v/v). At the same soil concentrations, cress showed negligible growth. The results suggest the need to use a battery of toxicity tests, in conjunction with chemical methods, in order to assess the quality of mine-contaminated soils correctly.

Pilot experiments with electrodialysis and ozonation for the production of a fertiliser from urine

Pilot tests were performed with a process combination of electrodialysis and ozonation for the removal of micropollutants and the concentration of nutrients in urine. In continuous and batch experiments, maximum concentration factors up to 3.5 and 4.1 were obtained, respectively. The desalination capacity did not decrease significantly during continuous operation periods of several weeks. Membrane cleaning after 195 days resulted in approximately 35% increase in desalination rate. The Yeast Estrogen Screen (YES), a bioassay that selectively detects oestrogenic compounds, confirmed that about 90% of the oestrogenic activity was removed by electrodialysis. HPLC analysis showed that ibuprofen was removed to a high extent, while other micropollutants were below the detection limit. In view of the fact that ibuprofen is among the most rapidly transported micropollutants in electrodialysis processes, this result indicates that electrodialysis provides an effective barrier for micropollutants. Standardised plant growth tests were performed in the field with the salt solution resulting from the treatment by electrodialysis and subsequent ozonation. The results show that the plant height is comparable to synthetic fertilisers, but the crop yield is slightly lower. The latter is probably caused by volatilisation losses during field application, which can be prevented by improved application technologies.

The effect of organic materials on the mobility and toxicity of metals in contaminated soils

Organic materials such as compost are often proposed as suitable materials for the remediation of contaminated brownfield sites intended for soft end-use. In addition to vitalising the soil, they are also believed to immobilise metals thereby breaking contaminant-receptor pathways and reducing the ecotoxicity of the contaminants. However, some research has demonstrated contradictory effects between composts on metal immobilisation. In the present study, four different composts and a liming product containing organic matter (LimeX70) were tested to examine both their metal retention and toxicity reduction capabilities on three different metal contaminated soils. Leaching tests, a plant growth test with Greek cress ( Lepidium sativum), an earthworm ( Eisenia fetida) survival and condition test and a bacterial toxicity test using Vibrio fischeri were carried out. The leaching test results showed that spent mushroom compost caused an increase in metal concentration in the leachates, while LimeX70 caused a decrease. The variation in behaviour between different amendments for each soil was high, so a generic conclusion could not be drawn. Toxicity tests showed significant reduction of metal bioavailability and toxicity for Greek cress, earthworms and bacteria. The results also suggest that more research should be undertaken to understand the mechanisms involved in metal complexation using different types of organic matter, in order to optimise the use of organic materials like compost for soil remediation.

Ecotoxicological tests assessment of soils polluted by chromium (VI) or pentachlorophenol.

Carbon mineralisation and plant germination and growth (Lactuca sativa seeds) tests have been performed in two soils of different properties, experimentally spiked with pentachlorophenol (PCP) or Cr (VI), in concentrations between 0.001 and 1000 mg kg(-1). The evaluation has been done considering the following parameters of carbon transformation test: soil cumulative basal respiration after 14 days incubation, substrate induced respiration after 12 h of glucose addition, and, in the plant germination and growth test: number of germinated seeds, root elongation and total biomass produced. The most sensitive assay found in our work has been carbon mineralisation test, from which the lowest toxic concentrations were obtained (especially substrate induced respiration test). In the plant germination and growth test, the measurement of root elongation has shown the best sensitiveness, followed by plant biomass and seed germination numbers. Regarding the contaminants, the highest toxicity, considering the minimum concentration with toxic effect, has been found in PCP (0.01 mg kg(-1)) in C mineralisation test in the granitic soil. For Cr, the minimum concentration with toxic effect has been 0.1 mg kg(-1), also in the C mineralisation test and the granitic soil. The granitic soil has shown more vulnerability to the pollutants assayed in the respiration test, whereas the calcareous soil has shown more vulnerability in the plant germination and growth test.

Aluminum status of synthetic Al–humic substance complexes and their influence on plant root growth

Aluminum (Al)–humus complexes are abundant in the A horizons of non-allophanic Andosols and contribute to the unique properties of volcanic ash soils, such as high reactivity with phosphate ions and a low bulk density. Natural non-allophanic Andosols commonly show Al toxicity to plant roots. There have been very few studies examining the contribution of Al–humus complexes to the Al toxicity of plant roots, although the complexes are the probable source of the toxic Al. We extracted humic substances from the A horizon of a non-allophanic Andosol using NaOH solution and reacted the humic substances and partially neutralized the AlCl3 solution at three pH conditions (pH 4.0, 4.5 and 5.5) to prepare pure Al–humic substance complexes. The Al solubility study (equilibrium study in 10−2 mol L−1 CaCl2) and the Al release study (a stirred-flow method using 10−3 mol L−1 acetate buffer solution adjusted to pH 3.5) indicated that all the synthetic complexes easily and rapidly release monomeric Al into the liquid phase with slight changes in pH and ion strength, although the Al contents and their extent of polymerization are considerably different among the complexes. A plant growth test was conducted using a medium containing the Al–humic substance complexes and perlite mixture. Root growth in burdock (Arctium lappa) and barley (Hordeum vulgare L.) was reduced equally by all three complex media, and the roots showed the typical injury symptoms of Al toxicity. These results indicate that in soils dominated by Al–humus complexes the Al released from the Al–humus complexes, as well as the exchangeable Al adsorbed by soil minerals, is definitely toxic to plant roots.

Evaluation of chemical and ecotoxicological characteristics of biodegradable organic residues for application to agricultural land

The use of organic waste and compost as a source of organic matter and nutrients is a common practice to improve soil physico-chemical properties, meanwhile reducing the need for inorganic fertilisers. Official guidelines to assess sewage sludge and compost quality are mostly based on total metal content of these residues. Measurement of the total concentration of metals may be useful as a general index of contamination, but provides inadequate or little information about their bioavailability, mobility or toxicity when the organic residue is applied to the soil. However, ecotoxicity tests provide an integrated measure of bioavailability and detrimental effects of contaminants in the ecosystem. In the present study, three different types of biodegradable organic residues (BORs) have been considered: sewage sludge from municipal wastewater treatment (SS), compost from the organic fraction of unsorted municipal solid waste (MSWC), and garden waste compost (GWC). The BORs were subjected to chemical characterisation and total metal quantification (Cd, Cr, Cu, Ni, Pb and Zn), in order to verify their suitability for land application. Water leachability was determined through the DIN 38414-S4 method, while the modified BCR sequential extraction procedure was used for metal speciation. Ecotoxicity of the BORs was studied by direct and indirect bioassays. Direct toxicity bioassays were: plant growth tests with cress ( Lepidium sativum L.) and barley ( Hordeum vulgare L.), and earthworm ( Eisenia fetida) mortality. On the other hand, indirect exposure bioassays, with leachate from the residues, took into account: luminescent bacteria ( Vibrio fischeri), seed germination ( L. sativum and H. vulgare) and Daphnia magna immobilization. As far as total metal concentration is concerned, with particular reference to Zn, SS resulted neither suitable for the use in agriculture nor compatible to be disposed of as an inert material into landfill, according to the Directive 1999/31/EC. Zinc in SS was mainly present in exchangeable form (28.5%), appearing as highly bioavailable. As a consequence, SS exhibited either high ecotoxicity effects with the indirect exposure bioassays or significant mortality with the earthworm bioassay. Total content of metals in MSWC allowed its classification as “stabilised biowaste”, according to 2nd draft [DG Env.A.2. Working document of Biological treatment of biowaste — 2nd draft. Directorate-General Environment, Brussels, 12th February; 2001. accessed in: http://europa.eu.int/comm/environment/waste/facts_en.htm, at 10/09/2002] while leachate, on the basis of the concentration of these contaminants, could be classified as “inert waste”. This residue showed significant ecotoxicity effects with direct exposure bioassays as well as with the luminescent bacteria bioassay. However, it resulted less toxic than SS. Finally, GWC could be classified as a Class 2 compost, with no detectable toxic effects on the organisms used in the bioassays, except for the luminescent bacteria. In this case, an EC 50 of 73.0% was observed. Considering the results, the use of a battery of toxicity test in conjunction with chemical analysis should be suggested, in order to correctly assess possible environmental risks deriving from disposal or land application of biodegradable organic residues.

Environmental Degradation of Polyester Blends Containing Atactic Poly(3-hydroxybutyrate). Biodegradation in Soil and Ecotoxicological Impact

The degradation of poly[(R,S)-3-hydroxybutyrate], a-PHB, binary blends with natural PHB (n-PHB) and poly(L-lactic acid), PLLA, respectively, has been investigated in soil. In such a natural environment, a-PHB blend component was found to biodegrade. The degradation of a-PHB-containing blends proceeded faster than that of respective plain n-PHB and PLLA. The molecular weight decrease of the n-PHB component was higher, while the same rate of bioerosion of both components was observed for the a-PHB/n-PHB binary blend. For the a-PHB blend with PLLA, the weight loss was accompanied by blend composition changes and the decrease of a-PHB content. However, the PLLA molecular weight decrease was lower in the blend in comparison with the plain PLLA sample. The increase of the number of microorganisms particularly observed for the soil where binary blends were incubated indicates that microbial degradation of a-PHB takes place. The terrestrial plant growth test (cress and barley) demonstrates no environmental toxicity of the materials studied.

Ecological Risk Assessment of Alfalfa Medicago Varia L.) Genetically Engineered to Express a Human Metallothionein (hMT) Gene

The objectives of these studies were two-fold: (1) to determine efficacy of low and high expression hMT gene constructs by assessing accumulation of Cu in shoots of parental and transgenic plants of alfalfa (Medicago varia L.) exposed to different concentrations of CuSO4 by addition of CuSO4 solutions to soil and (2) to identify potential unintended effects of the genetic engineering on root and shoot biomass, shoot nutrient content, arbuscular mycorrhizal infection and on the metabolic functions of microbial communities in the rhizosphere. In the absence of exogenous CuSO4 additions to soil shoot biomass and the macronutrient (C, P, K, Ca, Mg and N) content of plants expressing hMT were not significantly different from the parental control. In the 0.5 mM and 1.0 mM CuSO4 treatments transgenic plants expressing the commonly used transgenic β-glucuronidase (GUS) marker had significantly higher Fe content than the parental genotype. Significant differences were observed in the carbon substrate utilization patterns of rhizosphere microbial communities among the transgenic plants; no significant differences were observed in the percent mycorrhizal infection of parental and transgenic plants. Shoot biomass increased significantly in all genotypes treated with 0.5 mM CuSO4 and decreased in all genotypes at CuSO4 concentrations of 1.5 mM and 2.0 mM. Root dry weights decreased significantly in all genotypes at concentrations of 1.0 mM, 1.5 mM and 2.0 mM CuSO4. The largest decreases in root dry weight were observed in hMT genotypes grown in soil treated with 1.5 and 2.0 mM CuSO4. In plants treated with 1.5 mM CuSO4, shoots of transgenic plants expressing the hMT gene accumulated nominally, but not statistically significantly higher levels of Cu in shoot tissue. Our results were surprising with regard to lack of sufficient efficacy of the current hMT constructs for significant accumulation of Cu from soil treated with CuSO4. However, our results suggest the utility of applying adverse levels of CuSO4 or other environmental stressors to identify potential unintended effects of genetic engineering that may not be apparent under typically more optimal plant growth test conditions.

Cadmium phytotoxicity: Quantitative sensitivity relationships between classical endpoints and antioxidative enzyme biomarkers

In this work, cadmium phytotoxicity and quantitative sensitivity relationships between different hierarchical endpoints in plants cultivated in a contaminated soil were studied. Thus, germination rate, biomass growth and antioxidative enzyme activity (i.e. superoxide dismutase, peroxidase, catalase and glutathione reductase) in three terrestrial plants ( Avena sativa L., Brassica campestris L. cv. Chinensis, Lactuca sativa L. cv. hanson) were analyzed. Plant growth tests were carried out according to an International Standard Organization method and the results were analyzed by ANOVA followed by Williams' test. The concentration of Cd 2+ that had the smallest observed significant negative effect (LOEC) on plant biomass was 6.25, 12.5 and 50 mg Cd/kg dry soil for lettuce, oat and Chinese cabbage, respectively. Activity of all enzymes studied increased significantly compared to enzyme activity in plant controls. For lettuce, LOEC values (mg Cd/kg dry soil) for enzymic activity ranged from 0.05 (glutathione reductase) to 0.39 (catalase). For oat, LOEC values (mg Cd/kg dry soil) ranged from 0.19 (for superoxide dismutase and glutathione reductase) to 0.39 (for catalase and peroxidase). For Chinese cabbage, LOEC values (mg Cd/kg dry soil) ranged from 0.19 (peroxidase, catalase and glutathione reductase) to 0.39 (superoxide dismutase). Classical (i.e. germination and biomass) and biochemical (i.e. enzyme activity) endpoints were compared to establish a sensitivity ranking, which was: enzyme activity > biomass > germination rate. For cadmium-soil contamination, the determination of quantitative sensitivity relationships (QSR) between classical and antioxidative enzyme biomarkers showed that the most sensitive plant species have, generally, the lowest QSR values.

Toxicity of heavy metals in soil assessed with various soil microbial and plant growth assays: A comparative study

Abstract-Elevated metal concentrations in soils can disturb the soil ecosystem; thus, researchers strive to identify the most sensitive assay for detection of the early signs of toxicity. The purpose of the present study was to compare eight different ecotoxicological endpoints on the same set of metal-contaminated soils that were collected from seven series of soils sampled during field trials. The endpoints are based on three microbial assays (potential nitrification rate [PNR], substrate-induced respiration [SIR], and basal respiration [BR]) and two plant growth tests, one of which included symbiotic N fixation. The overall sensitivity of the endpoints to detect statistically significant adverse effects ranked as follows: PNR > SIR (lag time) > plant yield and N fixation > SIR (respiration after 24 and 48 h) > BR. The lowest adverse effect concentrations were found with the PNR at 7 mg kg(-1) of Cd and 107 mg kg(-1) of Zn. The variability of these endpoints among different uncontaminated soils was additionally assessed on 14 soil samples. That variability showed a strong correlation with sensitivity scores, illustrating that metal-sensitive endpoints have a large natural variability. We question the ecological relevance of highly sensitive microbial assays, because they tend to have a large natural variability. The identification of toxicity in the field requires endpoints that are highly sensitive and that do not vary greatly among soils (i.e., robust); however, no such endpoint was found in the present study. The endpoints that combined average sensitivity and robustness were SIR (lag time), clover yield, and N fixation in clover.

Assessing the pathogenic effect ofFusarium, Geosmithia andOphiostoma fungi from broad-leaved trees

Phytopathogenic effect of Geosmithia pallida, G. langdonii, Ophiostoma grandicarpum, O. querci, two isolates of O. piceae, and two isolates of Fusarium solani was compared using plant growth test (stem and root length of garden cress plants seeded on mycelium-covered potato carrot agar); Ophiostoma spp. and F. solani were isolated from oak, Geosmithia spp. from galleries of Scolytus intricatus on beech. All fungi inhibited more the root elongation than that of stems. F. solani led to plant collapse after briefly stimulating the growth of stem and in one case also root. G. langdonii inhibited stem and root growth to 20% and led to plant collapse. G. pallida inhibited root growth to 25% whereas stem growth was almost unimpaired. Ophiostoma spp. reduced stem growth to approximately 60-80% and root growth to 25-60%. O. piceae and O. querci caused plant collapse after 15-20 d.

Development and Application of a Two-Tier Diagnostic Test for High School Students’ Understanding of Flowering Plant Growth and Development

This study involved the development and application of a two-tier diagnostic test measuring students’ understanding of flowering plant growth and development. The instrument development procedure had three general steps: defining the content boundaries of the test, collecting information on students’ misconceptions, and instrument development. Misconception data were collected from interviews and multiple-choice questions with open response answers. The data were used to develop 13 two-tier multiple-choice items. The conceptual knowledge examined was flowering plant life cycles, reproduction, precondition of germination, plant nutrition, and mechanism for growth and development. The diagnostic instrument was administered to 477 high school students. The correlation coefficient of test-retest was 0.75. Difficulty indices ranged from 0.24 to 0.82, and discrimination indices ranged from 0.32 to 0.65. Results of the Flowering Plant Growth and Development Diagnostic Test suggested that students did not acquire a satisfactory understanding of plant growth and development concepts. Nineteen misconceptions were identified through analysis of the items that could inform biology instruction and resource.

Aerobic composting of chips from clear-cut trees with various co-materials

Swollen chips made from trees felled during clear-cutting were composted with various organic and inorganic materials in an aerobic composting reactor for 5 months and then piled for 5 months. The organic materials included chicken feces, urea, nitrogenous lime (calcium cyanamide, manure), and material rapidly composted from food garbage in 24-h bacterial fermentation, while the inorganic materials were coal ash and volcanic ash. In this paper, we first attempt to estimate the quality and degree of maturity of each compost from its chemical properties. Furthermore, we try to calculate the maturity of the fermented wood chip composts from their mixture ratio of the initial materials by multiple linear regression analysis. We measured changes in the C/N ratio, nitrate nitrogen (NO 3-N) content, percentage of humic acid in the alkali soluble fraction (PQ), cation exchange capacity, pH, and EC during the composting period. The degrees of maturity of the composts were estimated via a plant growth test using Chinese cabbage. We found that the CN ratio, NO 3-N concentration, and PQ were suitable for estimating the degree of maturity of wood chip composts. For maturity, the CN ratio should be less than 14, the PQ more than 66.2, and the NO 3-N concentration more than 853 mg kg −1. We devised an equation to estimate the degree of maturity after 10 months by a multiple linear regression analysis from the mixing ratio of wood chips and the co-composted materials. From the multiple linear regression analysis, the above three indices of compost maturity could be estimated from the mixing ratio of the initial materials. This equation should enable us to determine the degree of compost maturity after 10 months based on the initial mixing ratio.

Amelioration of Composting Process by Fertilizers

Six different composts, four of them produced from oil palm residues and two commercial composts, were studied for their stability and quality. The composts were analyzed for their total C, total N, C/N ratio, mineral N, nitrogen mineralization index, CO2 production, biomass C, specific respiration rate, germination rate, pH values and plant growth index. Composts of oil palm (Elaeis guineesis) trunk and oil palm empty fruit bunch treated with N and P showed good stability and higher quality as compared to other composts. Their C/N ratios were the lowest (14-18) but their pH values were comparatively low (4.6-6.4). They had a good amount of total N (0.95-1.84%) with higher nitrate than ammonium nitrogen. The specific respiration rates were very low (0.25-0.63) which is an indication that these two composts are stable. Plant growth test supported conclusions based on the microbiological tests. Their properties were considered as suitable for planting media application. Compost quality can be further improved by adding bunch ash, which has high values of pH and is also a by-product from the oil palm industry. The applied nitrogen mineralization index could not be used with all kinds of composts as stability index. Both of the commercial composts showed high C/N ratios (34-68) and very low values of total and mineral nitrogen compared to oil palm residues composts. They were considered having low nutritional value and not recommended for planting media application.

Preparation and Isolation of Oligogalacturonic Acids and Their Biological Effects in Cockscomb (Celosia argentea L.) Seedlings

A mixture of oligogalacturonic acids, the partial degradation substances of polygalacturonic acid, promoted shoot growth in cockscomb (Celosia argentea L.) seedlings, which generally had a high sensitivity for growth-promoting substances. The effect of the mixture of oligogalacturonic acids on shoot growth of cockscomb was higher than that of the polygalacturonic acid at concentrations above 30 ppm. These oligomers were loaded onto an anion exchange column, DEAE Sephadex A-25, and separated into individual oligomer sizes using the NH4HCO3 eluent system. This separation method has the advantage of using NH4HCO3 as the eluent solution; NH4HCO3 in the sample solution is effectively removed by lyophilization. Each of the isolated oligogalacturonic acids gave a single band on a fluorophore-assisted carbohydrate electrophoresis (FACE), and they showed the m/z value which corresponded to their molecular ion peaks [M-H]- on a fast atom bombardment mass spectrometry (FAB-MS) analysis. These results showed that the successive chromatography method used in this study is well suited for the preparation of oligogalacturonic acid for the plant growth test. Furthermore, we showed that the effective degree of polymerization (DP) of oligogalacturonic acid was around 8 on shoot growth of cockscomb seedlings, and the effects of both smaller and larger oligogalacturonic acids were slightly lower than that of octa-galacturonide. Octa-galacturonide promoted shoot growth of cockscomb at concentrations above 10 mM, and showed a 66% promotion at the most effective concentration of 300 mM. Root growth was slightly inhibited at concentrations above 300 mM. These results suggest that DP around 8 of oligogalacturonic acids has the function to control shoot growth in cockscomb as a growth-promoting substance.

Relevance of soil storage to biomass development, N-mineralisation and microbial activity using the higher plant growth test, ISO 11269-2, for testing of contaminated soils

Higher plant growth tests are recommended to be used for testing soils and soil materials for their ecotoxicological properties. Soil samples from two different sites (LUFA Speyer Standard soil and BBA Berlin, Dahlem) were taken fresh from the field or from a storage site and used in the higher plant growth test, ISO 11269-2. Biomass response after a 3 week growth period of oat ( Avena sativa L.) and wild turnip ( Brassica rapa ssp. rapa (DC.) Metzg.) was taken as test parameter to determine the influence of long term storage and various pre-treatments selected to prepare soil for being used as a control or reference soil. Tests were designed to allow for additional testing of microbial activity (dehydrogenase activity (DHA)), potential nitrification and supply of plant-available nitrogen (NO 3-N content) in the test substrates. Soil stored for a long time period under air-dry conditions (BBA-soil) or at conditions maintaining a low moisture content (LUFA-soil) had low microbial activity but a sufficiently high level of plant-available nitrogen (NO 3-N) and yielded good biomass increase after the growth period. Field soil used subsequently after sampling in the test had high microbial activity during the whole test period but low levels of NO 3-N providing only low amounts of nitrogen available for the early growth stages of the test species, which in addition decreased rapidly over the test period due to uptake by the test plants and to the microbial activity. No significant differences were observed between fresh soil pretreated differently over a period of time and soil used immediately after sampling in the test if pre-treatment conditions were appropriate to maintain microbial activity. Spreading stored soil on a field plot and keeping it for at least 4 weeks under field conditions resulted in similar biomass response as found with soil sampled fresh from the field. The effect of storage on the result of a soil quality characterization essay is finally demonstrated.

Biodegradation of lactic acid based polymers under controlled composting conditions and evaluation of the ecotoxicological impact.

The biodegradability of lactic acid based polymers was studied under controlled composting conditions (CEN prEN 14046), and the quality of the compost was evaluated. Poly(lactic acids), poly(ester-urethanes), and poly(ester-amide) were synthesized and the effects of different structure units were investigated. The ecotoxicological impact of compost samples was evaluated by biotests, i.e., by the Flash test, measuring the inhibition of light production of Vibrio fischeri, and by plant growth tests with cress, radish, and barley. All the polymers biodegraded to over 90% of the positive control in 6 months, which is the limit set by the CEN standard. Toxicity was detected in poly(ester-urethane) samples where chain linking of lactic acid oligomers had been carried out with 1,6-hexamethylene diisocyanate (HMDI). Both the Flash test and the plant growth tests indicated equal response to initial HMDI concentration in the polymer. All other polymers, including poly(ester-urethane) chain linked with 1,4-butane diisocyanate, showed no toxicological effect.

A Novel Method For Determining Phytotoxicity In Composts

Phytotoxicity of compost in various degrees of maturity has been extensively reported. It is usually determined by a seed germination index assay or by plant growth tests. The main deficiency of these tests is the length of time required for their performance. Therefore, we tested new biosensor techniques in this study based on oxygen consumption and fluorescence measurements of isolated freshly suspended or lyophilized chloroplast thylakoids (Vicia faba L.). These methods were compared with traditional phytotoxicity tests. The new systems proved to be suitable for evaluating the function of the photosynthetic electron transport (PET) between photosystem II and photosystem I. When PET was inhibited by phytotoxic substances, fluorescence increased whereas oxygen consumption decreased. Germination index data as well as plant growth bioassay data exhibited high correlations with data obtained from the novel biosensor techniques. Thus, we propose application of these methodologies based on freshly isolated or lyophilized chloroplast membranes, as rapid and sensitive tests for compost phytotoxicity.

Evaluating Quality and Suppressiveness Of Austrian Biowaste Composts

Twenty-one composts from source separated household biowaste plus other organic materials were evaluated for their biomaturity. The major part of the samples proved to be mature and enhancing plant development in a plant growth assay with cress. In addition to the standard physical and chemical parameters, water soluble C/N, humic/fulvic acid ratio, ratio of optical absorbances at 400 nm (E4) and 600 nm (E6) and cation exchange capacity were determined. None of these parameters was correlated with plant growth. Thus neither was suitable as an indicator to predict biomaturity in terms of growth enhancement and lack of phytotoxicity. The results of a germination test with compost extract were well correlated with the results of the plant growth test. Composts were suppressive to Pythium damping-off. The extent of suppressiveness was connected with the composts' content of organic matter.