Plant-based Systems Research Articles

Morphological and Chemical Evaluations of Leaf Surface on Particulate Matter2.5 (PM2.5) Removal in a Botanical Plant-Based Biofilter System.

Particulate matter has been increasing worldwide causing air pollution and serious health hazards. Owing to increased time spent indoors and lifestyle changes, assessing indoor air quality has become crucial. This study investigated the effect of watering and drought and illumination conditions (constant light, light/dark cycle, and constant dark) on particulate matter2.5 (PM2.5) removal and surface characterization of leaf in a botanical plant-based biofilter system. Using Ardisia japonica and Hedera helix as experimental plants in the plant-based biofilter system, PM2.5, volatile organic carbon, and CO2, as the evaluators of indoor air quality, were estimated using a sensor. Morphological and chemical changes of the leaf surface (i.e., roughness and wax) associated with PM2.5 removal were characterized via scanning electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The highest PM2.5 removal efficiency, stomata closure, high leaf roughness, and wax layer were observed under drought with constant light condition. Consequently, PM2.5 removal was attributed to the combined effect of leaf roughness and wax by adsorption rather than stomatal uptake. These results suggest that operating conditions of indoor plant-based biofilter system such as watering (or drought) and illumination may be applied as a potential strategy for enhancing PM2.5 removal.

Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves

BackgroundIn the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine, as three common plant growth regulators, on the plant biomass and efficiency of transient expression during the process of transient expression in Nicotiana benthamiana L. leaves.ResultsWith the increase of the concentration of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine (from 0.1 to 1.6 mg/L), the fresh weight, dry weight, and leaf area of the seedlings increased first and then returned to the levels similar to the controls (without chemical treatment). The treatment with α-naphthalene acetic acid at 0.2 and 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.4 mg/L α-naphthalene acetic acid and was increased about by 19%, compared to the controls. Gibberellins3 at 0.1–0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.2 mg/L gibberellins3 and was increased by 25%. However, the application of 6-benzyladenine led to decrease in the level of transient expression of green fluorescent protein.ConclusionsThe appropriate plant growth regulators at moderate concentration could be beneficial to the expression of foreign genes from the Agrobacterium-mediated transient expression system in plants. Thus, appropriate plant growth regulators could be considered as exogenous components that are applied for the production of recombinant protein by plant-based transient expression systems.

It's time for healthcare professionals to demand a plant-based food system.

Diet-related diseases are at an all-time high and the latest UN Intergovernmental Panel on Climate Change (IPCC) report on climate change has been called a ‘code red for humanity’. These two issues are intimately related. Our food system is at the centre of three global crises: health, climate, and ecology. We also have a crisis of ethics whereby more than a billion people remain hungry despite the production of enough food to feed two planets. If everyone ate a diet typical of the wealthiest nations, we would need seven planets to feed the world. We have a simple solution in plain sight. A healthy plant-based diet (PBD) would not only improve global health, preventing an estimated 1 in 5 deaths, but would also keep the food system within the planetary boundaries necessary to avert climate disaster, while also promoting biodiversity and feeding more people. The Planetary Health Plate created by the Eat-Lancet Commission is predominantly plant based, with more than 85% of energy derived from healthy plant foods, and acknowledges that a 100% PBD is not only nutritionally …

Integrated Pest Management Protocols for Space-Based Bioregenerative Life Support Systems

Human missions to the Moon and Mars will necessarily increase in both duration and complexity over the coming decades. In the past, short-term missions to low-Earth orbit (LEO) or the Moon (e.g., Apollo) utilized physiochemical life support systems for the crews. However, as the spatial and temporal durations of crewed missions to other planetary bodies increase, physiochemical life support systems become burdened with the requirement of frequent resupply missions. Bioregenerative life support systems (BLSS) have been proposed to replace much of the resupply required of physiochemical systems with modules that can regenerate water, oxygen, and food stocks with plant-based biological production systems. In order to protect the stability and productivity of BLSS modules (i.e., small scale units) or habitats (i.e., large scale systems), an integrated pest management (IPM) program is required to prevent, mitigate, and eliminate both insect pests and disease outbreaks in space-based plant-growing systems. A first-order BLSS IPM program is outlined herein that summarizes a collection of protocols that are similar to those used in field, greenhouse, and vertical-farming agricultural systems. However, the space environment offers numerous unusual stresses to plants, and thus, unique space-based IPM protocols will have to be developed. In general, successful operation of space-based BLSS units will be guided by IPM protocols that (1) should be established early in the mission design phase to be effective, (2) will be dynamic in nature changing both spatially and temporally depending on the successional processes afoot within the crewed spacecraft, plant-growing systems, and through time; and (3) can prevent insect/phytopathology outbreaks at very high levels that can approach 100% if properly implemented.

Plant-Based Colloidal Delivery Systems for Bioactives.

The supplementation of plant-based foods and beverages with bioactive agents may be an important strategy for increasing human healthiness. Numerous kinds of colloidal delivery systems have been developed to encapsulate bioactives with the goal of improving their water dispersibility, chemical stability, and bioavailability. In this review, we focus on colloidal delivery systems assembled entirely from plant-based ingredients, such as lipids, proteins, polysaccharides, phospholipids, and surfactants isolated from botanical sources. In particular, the utilization of these ingredients to create plant-based nanoemulsions, nanoliposomes, nanoparticles, and microgels is covered. The utilization of these delivery systems to encapsulate, protect, and release various kinds of bioactives is highlighted, including oil-soluble vitamins (like vitamin D), ω-3 oils, carotenoids (vitamin A precursors), curcuminoids, and polyphenols. The functionality of these delivery systems can be tailored to specific applications by careful selection of ingredients and processing operations, as this enables the composition, size, shape, internal structure, surface chemistry, and electrical characteristics of the colloidal particles to be controlled. The plant-based delivery systems discussed in this article may be useful for introducing active ingredients into the next generation of plant-based foods, meat, seafood, milk, and egg analogs. Nevertheless, there is still a need to systematically compare the functional performance of different delivery systems for specific applications to establish the most appropriate one. In addition, there is a need to test their efficacy at delivering bioavailable forms of bioactives using in vivo studies.

Robust Agrobacterium-Mediated Transient Expression in Two Duckweed Species (Lemnaceae) Directed by Non-replicating, Replicating, and Cell-to-Cell Spreading Vectors.

Plant-based transient expression systems have recognized potential for use as rapid and cost-effective alternatives to expression systems based on bacteria, yeast, insect, or mammalian cells. The free-floating aquatic plants of the Lemnaceae family (duckweed) have compact architecture and can be vegetatively propagated on low-cost nutrient solutions in aseptic conditions. These features provide an economically feasible opportunity for duckweed-based production of high-value products via transient expression of recombinant products in fully contained, controlled, aseptic and bio-safe conditions in accordance with the requirements for pharmaceutical manufacturing and environmental biosafety. Here, we demonstrated Agrobacterium-mediated high-yield transient expression of a reporter green fluorescent protein using deconstructed vectors based on potato virus X and sweet potato leaf curl virus, as well as conventional binary vectors, in two representatives of the Lemnaceae (Spirodela polyrhiza and Landoltia punctata). Aseptically cultivated duckweed populations yielded reporter protein accumulation of >1 mg/g fresh biomass, when the protein was expressed from a deconstructed potato virus X-based vector, which is capable of replication and cell-to-cell movement of the replicons in duckweed. The expression efficiency demonstrated here places duckweed among the most efficient host organisms for plant-based transient expression systems, with the additional benefits of easy scale-up and full containment.

Nature Based Solution for indoor air quality treatment

Plants have the ability to absorb and degrade VOCs (volatile organic compounds). Foliage can intercept particulate matter (PM) and thus, help to reduce its concentration in the air. Plants can be used as filters in indoor conditions adding an ecosystem service to the decorative purpose. A plant-based air filtration system that actively improves indoor air quality has been developed and installed at a students’ residence at Brunel University, London. This unit replaces an existing window with a mini-greenhouse containing upwards of 30 plants and is connected to an air circuit to treat the indoor air. A monitoring plan is collecting data on the performance of the solution until at least the end of 2021. Preliminary results are presented, which indicate good effectiveness at reducing tVOCs and lower efficiency at reducing PM.

Recent advances on the removal of phosphorus in aquatic plant-based systems

Phosphorus (P) is a vital nutrient for the ecosystems and its excess in wastewater streams leads to some environmental issues such as extensive algae growth (eutrophication). Phytoremediation is a green technology that is based on the combined actions of plants and their associated microbial groups to remove and transfer the toxic compounds in surface water, groundwater and soil. Aquatic plants are widely used for the remediation of contaminated rivers, eutrophic lakes, and other water bodies. In the past three decades, free-floating, submerged, emergent macrophytes and microalgae species have been used for P removal in aquatic plant-based systems such as constructed wetlands (CWs). This paper reviews the recent studies on the potential of aquatic plants such as free-floating, submerged, and emergent plants, and also microalgae for removal of P in different types of wastewaters. Several parameters such as plant species, hydraulic retention time, temperature, type of CWs, effluent concentration, and seasonal changes have effects on P removal. Based on the findings, some of the species such as Azolla and water hyacinth had the highest uptake ability up to 90% while algae species such as Chlorella showed about 70% of P removal. In addition, the mixed culture of aquatic plants can increase P removal if the interaction of the species is considered before cultivation in CWs. • The potential of different aquatic plants on phosphorous (P) removal is reviewed. • Azolla and water hyacinth had the highest P removal ability. • The algae are sensitive to the low P effluent concentration and the removal is quite low. • Submerged and emergent plants showed different removal patterns in P removal. • The recovered P can be used in beneficial applications such as biofertilizers.

The methods behind transgenic plant production: a review

The ability to insert a gene into a plant’s nuclear or chloroplast genome enables the transformation of higher plants (e.g. tobacco, Arabidopsis thaliana, potato, tomato, and banana) into Bioreactors for the production of plant-derived pharmaceuticals. Biopharmaceuticals are generally produced on a commercial basis by scale fermentation in bacteria, yeast, or animal cells. Several plant-derived pharmaceuticals have undergone clinical trials and are close to market authorization, with antibodies and vaccines being the front runners. Plant-derived vaccines have been produced using recombinant plant viruses as transgenic expression vectors and Agrobacterium tumefaciens transformation systems. During the last decade, several efficient plant-based expression systems have been examined, and more than 100 recombinant proteins, including plant-derived vaccine antigens. Besides, regulatory protocols are slowing down production. Industry requirements and public acceptance of the technology are important aspects in establishing successful products. This paper reviews the current status of development in the area of biopharmaceuticals and vaccines produced from transgenic plants.

Physiological responses of orchids to prolonged clinorotation

Creation of plant-based bioregenerative life support systems is crucial for future long-duration space exploring missions. Microgravity is one of the major stresses affecting plant growth and development under space flight conditions. Search for higher plant genotypes resilient to microgravity as well as revealing of biological features which could be used as markers of such resilience is rather urgently needed. The objective of this study was to analyze physiological and biochemical responses of three orchid species representing different life forms (terrestrial and epiphytic), growth types (monopodial and sympodial) and pathways of CO2 fixation to long-term (24 months) clinorotation which modeled the combined effect of two stress factors: hermetic conditions and microgravity. Three years old meristematic orchids Cypripedium flavum, Angraecum eburneum, Epidendrum radicans, representing different life forms, types of branching shoot system and pathways of CO2 fixation, were used as test-plants. The microgravity was simulated using three-dimensional (3-D) clinostat equipped with two rotation axes placed at right angles (rotation frequency was 3 rpm) in controlled conditions of air temperature, illumination, air humidity and substrate moisture. The control plants were grown in the similar plastic vessels but not hermetically sealed and without clinorotating in the same environmental conditions. The vital state of the test plants was assessed using characteristics of mineral nutrition, content of photosynthetic pigments, free amino acids, soluble proteins, DNA and RNA, enzymatic and non-enzymatic antioxidants. The results of this study confirmed that orchids grown under simulated microgravity and kept in hermetically-sealed vessels were subjected to oxidative stress, which could be responsible for the observed inhibition of basic physiological processes such as mineral nutrition, metabolism of aminoacids, protein biosynthesis and photosynthesis. Monopodial orchids C. flavum and A. eburneum demonstrated better adaptation to prolonged clinorotation as compared to sympodial E. radicans. In particular, the latter demonstrated some stimulation of mineral nutrition processes (i.e. K, N, Fe, Mn, Zn accumulation), content of photosynthetic pigments, proline and superoxide dismutase activity. Long-lasting clinorotation induced adaptive changes of antioxidant systems in the studied orchids (e.i. increase in carotenoids and proline content and stimulation of superoxide dismutase activity), which helped to maintain the main physiological functions at stable level in the above-mentioned stressful conditions. The following biochemical characteristics in the studied orchids could be considered as markers of resilience to simulated microgravity and hermetic conditions: 1) an increase in the accumulation of non-enzymatic (proline, carotenoids) and enzymatic antioxidants (superoxide dismutase); 2) ability to maintain stable balance of mineral nutrients; 3) increase in the content of photosynthetic pigments; 4) increase in the content of proteinogenic amino acids and soluble proteins; 5) increase in the DNA content or RNA/DNA ratio. Our studies have also demonstrated a correlation between orchid ecomorphological characteristics such as type of branching with their adaptive responses to prolonged clinorotation. We observed no correlation between the studied life form of orchids, ecotype or the pathway of CO2 fixation and their resilience to prolonged clinorotation. This research can be a starting point for studying the relationships between ecomorphological features of various orchids and their resilience to microgravity conditions in the search for biological markers of microgravity tolerance in species of higher plants.

Physiological responses of orchids to prolonged clinorotation

Creation of plant-based bioregenerative life support systems is crucial for future long-duration space exploring missions. Microgravity is one of the major stresses affecting plant growth and development under space flight conditions. Search for higher plant genotypes resilient to microgravity as well as revealing of biological features which could be used as markers of such resilience is rather urgently needed. The objective of this study was to analyze physiological and biochemical responses of three orchid species representing different life forms (terrestrial and epiphytic), growth types (monopodial and sympodial) and pathways of CO2 fixation to long-term (24 months) clinorotation which modeled the combined effect of two stress factors: hermetic conditions and microgravity. Three years old meristematic orchids Cypripedium flavum, Angraecum eburneum, Epidendrum radicans, representing different life forms, types of branching shoot system and pathways of CO2 fixation, were used as test-plants. The microgravity was simulated using three-dimensional (3-D) clinostat equipped with two rotation axes placed at right angles (rotation frequency was 3 rpm) in controlled conditions of air temperature, illumination, air humidity and substrate moisture. The control plants were grown in the similar plastic vessels but not hermetically sealed and without clinorotating in the same environmental conditions. The vital state of the test plants was assessed using characteristics of mineral nutrition, content of photosynthetic pigments, free amino acids, soluble proteins, DNA and RNA, enzymatic and non-enzymatic antioxidants. The results of this study confirmed that orchids grown under simulated microgravity and kept in hermetically-sealed vessels were subjected to oxidative stress, which could be responsible for the observed inhibition of basic physiological processes such as mineral nutrition, metabolism of aminoacids, protein biosynthesis and photosynthesis. Monopodial orchids C. flavum and A. eburneum demonstrated better adaptation to prolonged clinorotation as compared to sympodial E. radicans. In particular, the latter demonstrated some stimulation of mineral nutrition processes (i.e. K, N, Fe, Mn, Zn accumulation), content of photosynthetic pigments, proline and superoxide dismutase activity. Long-lasting clinorotation induced adaptive changes of antioxidant systems in the studied orchids (e.i. increase in carotenoids and proline content and stimulation of superoxide dismutase activity), which helped to maintain the main physiological functions at stable level in the above-mentioned stressful conditions. The following biochemical characteristics in the studied orchids could be considered as markers of resilience to simulated microgravity and hermetic conditions: 1) an increase in the accumulation of non-enzymatic (proline, carotenoids) and enzymatic antioxidants (superoxide dismutase); 2) ability to maintain stable balance of mineral nutrients; 3) increase in the content of photosynthetic pigments; 4) increase in the content of proteinogenic amino acids and soluble proteins; 5) increase in the DNA content or RNA/DNA ratio. Our studies have also demonstrated a correlation between orchid ecomorphological characteristics such as type of branching with their adaptive responses to prolonged clinorotation. We observed no correlation between the studied life form of orchids, ecotype or the pathway of CO2 fixation and their resilience to prolonged clinorotation. This research can be a starting point for studying the relationships between ecomorphological features of various orchids and their resilience to microgravity conditions in the search for biological markers of microgravity tolerance in species of higher plants.

Capillary absorption spectroscopy for high temporal resolution measurements of stable carbon isotopes in soil and plant-based systems

Capillary Absorption Spectroscopy (CAS) is a relatively new analytical technique for performing stable isotope analysis. Here, we demonstrate the utility of CAS by recording and quantifying variation in 13C in controlled and biologically relevant applications. We calibrated CAS system response to increased 13CO2, with an observed ∼4‰ increase in measured Δ13C for each 0.03 ppm shift in 13CO2 concentration. We leveraged this calibration to quantify rates of biogeochemical processes using a 13C tracer. For example, we monitored microbial respiration of 13C-glucose within an agricultural soil at 10 s quantification intervals and results demonstrated 8.6% ± 0.4 of added glucose was converted to 13CO2 within 1.5 h of incubation. We expanded the demonstration by adapting a rhizobox to permit continuous monitoring of 13CO2 in a soil (as distinct from plant) headspace to track the timing and quantify respiration rates of fresh plant photosynthate and observed a 3.5 h delay between plant exposure to a13CO2 tracer and the first signs of respiration by soil biota. These experiments highlight CAS is effective in producing high temporal resolution quantification of 13CO2 and demonstrate potential applications.

Use of the DELTA Model to Understand the Food System and Global Nutrition

BackgroundIncreasing attention is being directed at the environmental, social, and economic sustainability of the global food system. However, a key aspect of a sustainable food system should be its ability to deliver nutrition to the global population. Quantifying nutrient adequacy with current tools is challenging. ObjectiveTo produce a computational model illustrating the nutrient adequacy of current and proposed global food systems. MethodsThe DELTA Model was constructed using global food commodity balance sheet data, alongside demographic and nutrient requirement data from UN and European Food Safety Authority sources. It also includes nutrient bioavailability considerations for protein, the indispensable amino acids, iron, and zinc, sourced from scientific literature. ResultsThe DELTA Model calculates global per capita nutrient availability under conditions of equal distribution and identifies areas of nutrient deficiency for various food system scenarios. Modeling the 2018 global food system showed that it supplied insufficient calcium (64% of demographically weighted target intake) and vitamin E (69%), despite supplying sufficient macronutrients. Several future scenarios were modeled, including variations in waste; scaling up current food production for the 2030 global population; plant-based food production systems; and removing sugar crops from the global food system. Each of these scenarios fell short of meeting requirements for multiple nutrients. These results emphasize the need for a balanced approach in the design of future food systems. ConclusionsNutrient adequacy must be at the forefront of the sustainable food system debate. The DELTA Model was designed for both experts and nonexperts to inform this debate as to what may be possible, practical, and optimal for our food system. The model results strongly suggest that both plant and animal foods are necessary to achieve global nutrition. The model is freely available for public use so that anyone can explore current and simulated global food systems.

Human Insulin: History, Recent Advances, and Expression Systems for Mass Production

The significant rise in the number of diabetic patients worldwide, as well as the development of new insulin delivery techniques such as inhalation or oral administration which require higher dosages, are expected to increase the demand for recombinant insulin. Current manufacturing technologies will be unable to fulfill the rising demand for inexpensive insulin due to their production capacity limitations and high production costs. Production of therapeutic recombinant insulin requires a suitable host organism with adequate post-translational modification and refolding machinery. E. coli and S. cerevisiae have been used extensively to make recombinant human insulin for medicinal applications. However, transgenic plants are particularly appealing expression systems as they can be used to synthesize huge amounts of insulin for human medicinal purposes. Plant-based expression systems have the potential for high-capacity insulin synthesis at a minimal cost. The significant production of biologically active proinsulin in seeds or leaves with long-term stability provides a low-cost technique to develop proinsulin for both injectable and oral administration. Recently, stem cell therapy is being utilized for the treatment of diabetes, as these cells are capable of differentiating into insulin producing cells. With the advancement of regenerative medicine research for different chronic diseases, treatment for type 1 diabetes mellitus has been reported. The current review concentrates on several biotechnological attributes applied to the rapid and mass synthesis of biologically active insulin and its analogs in microbes, various types of stem cells and transgenic crops.

Production of a Bacteria-like Particle Vaccine Targeting Rock Bream (Oplegnathus fasciatus) Iridovirus Using Nicotiana benthamiana.

Viral diseases are extremely widespread infections that change constantly through mutations. To produce vaccines against viral diseases, transient expression systems are employed, and Nicotiana benthamiana (tobacco) plants are a rapidly expanding platform. In this study, we developed a recombinant protein vaccine targeting the major capsid protein (MCP) of iridovirus fused with the lysine motif (LysM) and coiled-coil domain of coronin 1 (ccCor1) for surface display using Lactococcus lactis. The protein was abundantly produced in N. benthamiana in its N-glycosylated form. Total soluble proteins isolated from infiltrated N. benthamiana leaves were treated sequentially with increasing ammonium sulfate solution, and recombinant MCP mainly precipitated at 40–60%. Additionally, affinity chromatography using Ni–NTA resin was applied for further purification. Native structure analysis using size exclusion chromatography showed that recombinant MCP existed in a large oligomeric form. A minimum OD600 value of 0.4 trichloroacetic acid (TCA)-treated L. lactis was required for efficient recombinant MCP display. Immunogenicity of recombinant MCP was assessed in a mouse model through enzyme-linked immunosorbent assay (ELISA) with serum-injected recombinant MCP-displaying L. lactis. In summary, we developed a plant-based recombinant vaccine production system combined with surface display on L. lactis.

Transient protein expression systems in plants and their applications.

The production of recombinant proteins is important in academic research to identify protein functions. Moreover, recombinant enzymes are used in the food and chemical industries, and high-quality proteins are required for diagnostic, therapeutic, and pharmaceutical applications. Though many recombinant proteins are produced by microbial or mammalian cell-based expression systems, plants have been promoted as alternative, cost-effective, scalable, safe, and sustainable expression systems. The development and improvement of transient expression systems have significantly reduced the period of protein production and increased the yield of recombinant proteins in plants. In this review, we consider the importance of plant-based expression systems for recombinant protein production and as genetic engineering tools.

A Novel Plant-Produced Asialo-rhuEPO Protects Brain from Ischemic Damage Without Erythropoietic Action.

Mammalian cell-produced recombinant human erythropoietin (rhuEPOM) has been shown to be a multimodal neuroprotectant targeting an array of key pathological mechanisms in experimental stroke models. However, the rhuEPOM clinical trials were terminated due to increased risk of thrombosis, largely ascribed to its erythropoietic function. We recently took advantage of a plant-based expression system lacking sialylation capacity to produce asialo-rhuEPOP, a rhuEPO derivative without sialic acid residues. In the present study, we proved that asialo-rhuEPOP is non-erythropoietic by repeated intravenous injection (44μg/kg bw) in mice showing no increase in hemoglobin levels and red blood cell counts, and confirmed that it is non-immunogenic by measuring humoral response after immunizing the mice. We demonstrate that it is neuroprotective in a cerebral ischemia and reperfusion (I/R) mouse model, exhibiting ~ 50% reduction in cerebral infarct volume and edema, and significant improvement in neurological deficits and histopathological outcome. Our studies further revealed that asialo-rhuEPOP, like rhuEPOM, displays pleiotropic neuroprotective effects, including restoring I/R-interrupted mitochondrial fission and fusion proteins, preventing I/R injury-induced increase in mitophagy and autophagy markers, and inhibiting apoptosis to benefit nerve cell survival. Most importantly, asialo-rhuEPOP lacking erythropoietic activity and immunogenicity holds great translational potential as a multimodal neuroprotectant for stroke treatment.

Functional Characterization of Pembrolizumab Produced in Nicotiana benthamiana Using a Rapid Transient Expression System.

The striking innovation and clinical success of immune checkpoint inhibitors (ICIs) have undoubtedly contributed to a breakthrough in cancer immunotherapy. Generally, ICIs produced in mammalian cells requires high investment, production costs, and involves time consuming procedures. Recently, the plants are considered as an emerging protein production platform due to its cost-effectiveness and rapidity for the production of recombinant biopharmaceuticals. This study explored the potential of plant-based system to produce an anti-human PD-1 monoclonal antibody (mAb), Pembrolizumab, in Nicotiana benthamiana. The transient expression of this mAb in wild-type N. benthamiana accumulated up to 344.12 ± 98.23 μg/g fresh leaf weight after 4 days of agroinfiltration. The physicochemical and functional characteristics of plant-produced Pembrolizumab were compared to mammalian cell-produced commercial Pembrolizumab (Keytruda®). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis results demonstrated that the plant-produced Pembrolizumab has the expected molecular weight and is comparable with the Keytruda®. Structural characterization also confirmed that both antibodies have no protein aggregation and similar secondary and tertiary structures. Furthermore, the plant-produced Pembrolizumab displayed no differences in its binding efficacy to PD-1 protein and inhibitory activity between programmed cell death 1 (PD-1) and programmed cell death ligand 1 (PD-L1) interaction with the Keytruda®. In vitro efficacy for T cell activation demonstrated that the plant-produced Pembrolizumab could induce IL-2 and IFN-γ production. Hence, this proof-of-concept study showed that the plant-production platform can be utilized for the rapid production of functional mAbs for immunotherapy.

Plant-Based COVID-19 Vaccines: Current Status, Design, and Development Strategies of Candidate Vaccines.

The prevalence of the coronavirus disease 2019 (COVID-19) pandemic in its second year has led to massive global human and economic losses. The high transmission rate and the emergence of diverse SARS-CoV-2 variants demand rapid and effective approaches to preventing the spread, diagnosing on time, and treating affected people. Several COVID-19 vaccines are being developed using different production systems, including plants, which promises the production of cheap, safe, stable, and effective vaccines. The potential of a plant-based system for rapid production at a commercial scale and for a quick response to an infectious disease outbreak has been demonstrated by the marketing of carrot-cell-produced taliglucerase alfa (Elelyso) for Gaucher disease and tobacco-produced monoclonal antibodies (ZMapp) for the 2014 Ebola outbreak. Currently, two plant-based COVID-19 vaccine candidates, coronavirus virus-like particle (CoVLP) and Kentucky Bioprocessing (KBP)-201, are in clinical trials, and many more are in the preclinical stage. Interim phase 2 clinical trial results have revealed the high safety and efficacy of the CoVLP vaccine, with 10 times more neutralizing antibody responses compared to those present in a convalescent patient’s plasma. The clinical trial of the CoVLP vaccine could be concluded by the end of 2021, and the vaccine could be available for public immunization thereafter. This review encapsulates the efforts made in plant-based COVID-19 vaccine development, the strategies and technologies implemented, and the progress accomplished in clinical trials and preclinical studies so far.

'Anti-fugue' plant device, an innovative concept in gerontology

Plant features represent amodality of outdoor architecture that can be integrated into a project to take into account the particularities of patients and residents, and in particular those with cognitive disorders and risks of running away. The concept of a "plant-based security system" is an innovative and transposable prevention approach that reconciles the constitutional right to freedom of movement and security. A living concept illustrated by the Seilleraye EHPAD in Carquefou.