Abstract
BackgroundIn the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine, as three common plant growth regulators, on the plant biomass and efficiency of transient expression during the process of transient expression in Nicotiana benthamiana L. leaves.ResultsWith the increase of the concentration of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine (from 0.1 to 1.6 mg/L), the fresh weight, dry weight, and leaf area of the seedlings increased first and then returned to the levels similar to the controls (without chemical treatment). The treatment with α-naphthalene acetic acid at 0.2 and 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.4 mg/L α-naphthalene acetic acid and was increased about by 19%, compared to the controls. Gibberellins3 at 0.1–0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.2 mg/L gibberellins3 and was increased by 25%. However, the application of 6-benzyladenine led to decrease in the level of transient expression of green fluorescent protein.ConclusionsThe appropriate plant growth regulators at moderate concentration could be beneficial to the expression of foreign genes from the Agrobacterium-mediated transient expression system in plants. Thus, appropriate plant growth regulators could be considered as exogenous components that are applied for the production of recombinant protein by plant-based transient expression systems.
Highlights
Transient expression technology refers to the technology of introducing the target gene into recipient cells to establish a temporary high-efficiency expression system so that the target gene can be expressed in a relatively short time
In this study, by using the bean yellow dwarf virus (BeYDV)-derived replicating expression vector and green fluorescent protein (GFP) as a reporter, we investigated the effects of naphthalene acetic acid (NAA), GA3, and 6-BA, as three common Plant growth regulators (PGRs), on the plant biomass and efficiency of transient expression during the process of transformation in Nicotiana benthamiana L. leaves
Effect of NAA on growth and transient expression of Nicotiana benthamiana L. seedlings In this study, strong GFP expression was observed in the leaves that were infected with LBA4404 carrying
Summary
Transient expression technology refers to the technology of introducing the target gene into recipient cells to establish a temporary high-efficiency expression system so that the target gene can be expressed in a relatively short time. The transformed target gene by the transient expression technology is not integrated into the genome, Production of the desired protein via transient expression has obvious advantages, including less time with more protein expression, lower cost, and easy manipulation without any biosafety concerns, compared to develop stable transgenic lines with time-consuming procedures (Xia et al 2020). In the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins, and 6-benzyladenine, as three common plant growth regulators, on the plant biomass and efficiency of transient expression during the process of transient expression in Nicotiana benthamiana L. leaves
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
