Chemists at Northeastern University have adapted polyacrylamide gel electrophoresis (PAGE) to a capillary format. Gel electrophoresis allows analytical and preparative separations of peptides and nucleotides by molecular weight. And electrophoresis in capillaries narrower than about 80 μm gives rapid, reproducible, quantitatively reliable separations with potentially millions of theoretical plates (C&EN, March 7, page 28). Analytical chemistry professor Barry L. Karger of Northeastern described possible applications of the technique at the Pittsburgh Conference on Analytical Chemistry & Applied Spectroscopy, held last month in New Orleans. Working with staff scientist Aharon S. Cohen, visiting scholar Carlos Diez-Masa, and postdoctoral fellows Andras Guttman and Aran Paulus and supported by the National Science Foundation and Dow Chemical Co., Karger filled capillary tubes with aqueous solutions of acrylamide monomer, N,N' -methylenebis-(acrylamide) crosslinking agent, ammonium persulfate initiator, and t...
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