The diet, reproduction and activity time of bat species found in Ponta Grossa county, Campos Gerais region, were studied. Collections were conducted in four forest fragments, during 272 hours, on 48 m 2 of nets and roosting sites; the total capture effort was 1.52.10 3 h.m 2 . Eight species (247 individuals) were registered: Artibeus lituratus (Olfers, 1818), Sturnira lilium (E. Geoffroy, 1810), Desmodus rotundus (E. Geoffroy, 1810) (Phyllostomidae); Tadarida brasiliensis (Desmarest, 1819), Eumops auripendulus (Shaw, 1800) (Molossidae); Eptesicus brasiliensis (Shaw, 1800), Myotis nigricans (Schinz, 1821), and Histiotus velatus (I. Geoffroy, 1824) (Vespertilionidae). The Phyllostomidae family was the most frequently captured. Solanaceae, Moraceae, Piperaceae, and Rosaceae were found in the diet of frugivores; six orders of insects and the class Arachnida were found in the diets of insectivores. Pregnant females were found in September and October and lactating ones in November and December. The collection peak was reached in the second hour-and-a-half. Preservation of the regional forested and altered areas is required for survival of the local chiropterofauna.