This paper studies the fixed-time anti-synchronization (FTAS) of discontinuous reaction-diffusion neural networks (DRDNNs) with both time-varying coefficients and time delay. First, differential inclusion theory is used to deal with the influence caused by discontinuous activations. In addition, a new fixed-time convergence theorem is used to handle the time-varying coefficients. Second, a novel state-feedback control algorithm and integral state-feedback control algorithm are proposed to realize FTAS of DRDNNs. During the generalized (adaptive) pinning control strategy, a guideline is proposed to select neurons to pin the designed controller. Furthermore, we present several criteria on FTAS by using the generalized Lyapunov function method. Different from the traditional Lyapunov function with negative definite derivative, the derivative of the Lyapunov function can be positive in this paper. Finally, we give two numerical simulations to substantiate the merits of the obtained results.