Background: The productivity of blackgram is not adequate to meet the domestic demand of the growing Indian population. Consequently, there is an urgent need for enhancement of productivity through proper management practices. With this background, a research experiment was conducted to investigate the effect of liquid rhizobium with organic bio-stimulants on physiological and biochemical characters, antioxidant enzymes and yield of blackgram. Methods: A field experiment was laid out in randomized block design with three replications during 2021 (Kharif and Rabi) season. The treatments include 100% recommended dose of NPK along with foliar application of dasagavya, liquid rhizobium, fish amino acid, panchagavya, pink pigmented facultative methylotrophs (PPFM) and Sea weed extract with different concentration (1% and 3%, respectively) in addition to control. Organic bio stimulants were sprayed at 30 and 45 days after the sowing of blackgram. Result: The experiment results revealed that the application of 100% RDF+Liquid Rhizobium @ 1% registered maximum physiological and biochemical characters viz., CGR, total chlorophyll content, soluble protein content, nitrate reductase activity, catalase activity, peroxidase activity and number of root nodules plant-1, yield attributes viz., number of pods plant-1, number of seeds pod-1, grain yield (kg ha-1), haulm yield (kg ha-1). Correlation and Regression analysis also indicated that the yield attributes had a positive impact on the grain yield.