Statement of problemThe accuracy of a maxillomandibular relationship acquired by intraoral scanners (IOSs) has been previously analyzed; however, the impact of the interocclusal space on the accuracy of the maxillomandibular relationship remains unknown. PurposeThe purpose of this in vitro investigation was to evaluate the influence of the interocclusal space (0, 1, 2, 3, or 4 degrees of incisal opening in the articulator) on the accuracy of the maxillomandibular relationship captured with an IOS. Material and methodsMarkers were attached to the first molars and canines of maxillary and mandibular diagnostic casts, which were mounted on a semi-adjustable articulator, and digital scans were acquired (TRIOS 4). Both digital scans were duplicated 100 times and distributed into 5 groups depending on the incisal pin opening in the articulator (n=20): 0 (Group 0), 1 (Group 1), 2 (Group 2), 3 (Group 3), and 4 degrees (Group 4). In Group 0 (control), the casts were maintained in maximum intercuspation (MIP) with the incisal pin of the articulator set at 0 degrees. Then, a bilateral virtual occlusal record was acquired and automatically processed by using the IOS software program. A laboratory scanner (Medit T500) was used to digitize the mounted casts. The same procedures were completed in Groups 1, 2, 3, and 4 but with the incisal pin set at 1, 2, 3, and 4 degrees respectively. The interlandmark distances were used to calculate the discrepancies between the control and groups tested. One-way analysis of variance (ANOVA) and pairwise comparison Tukey HSD tests were used to inspect the data (α=.05). ResultsThe interocclusal space available when capturing the occlusal records affected the trueness of the maxillomandibular virtual relationship measured (P<.001). Group 0 (135 μm) obtained the highest distortion, while Group 3 (73 μm) and Group 4 (71 μm) showed the lowest distortion. Additionally, the interocclusal space available (P<.001) impacted the precision of the maxillomandibular virtual relationship measured. Group 0 (111 μm) obtained the highest distortion, while Group 4 (precision mean value of 59 μm) had the lowest distortion among the groups tested. ConclusionsThe interocclusal space available when acquiring virtual bilateral occlusal records using the IOS tested impacted the accuracy of the maxillomandibular relationship. The smallest available interocclusal space tested (maximum intercuspation) showed the worst trueness and precision mean values, while the group with the largest interocclusal space available had the highest trueness and precision mean values among the groups studied.