Picture Of Diversity Research Articles

Global meta‐analysis reveals the drivers of gut microbiome variation across vertebrates

AbstractShifts in gut microbial diversity and structure are one route by which vertebrate hosts adapt to local environmental conditions. However, recent studies have mostly been limited to a single species, small sample sizes, or restricted geographic ranges. Therefore, drawing a global picture of vertebrate gut microbiome diversity, community structure, and determinants for their adaptive shifts remains to be elucidated. We here collected 6508 samples from 113 vertebrate species covering diverse classes, feeding behaviors, and host habitats based on 16S rRNA gene sequencing. The results showed that host diet pattern had a significant impact on gut microbiome variation, which might drive taxonomic and functional contents of gut microbiome across vertebrates. Of note, the phylum Fusobacteria were enriched in carnivorous vertebrate gut while herbivorous vertebrate gut selectively increased the abundance of Verrucomicrobia. Also, climate factors were strongly associated with gut microbiome variation across vertebrates. Interestingly, we found that the abundance of microbiota belonging to Bacteroidetes increased gradually while the members from Proteobacteria showed a decreasing trend from high‐ to low‐latitude zones, potentially contributing to vertebrate adaptation to local climate condition. Additionally, we comprehensively deciphered the common antibiotic resistomes and their potential mobility between terrestrial vertebrate gut microbiome (n = 487) and their sympatric soil biological environment samples (n = 203) by integrating metagenomic sequencing datasets. Particularly, potential horizontal antibiotic resistance genes (e.g., bacA) transfers were detected between vertebrates gut microbiome and their sympatric soil biological environment. Together, our findings provide new evidence of how external environmental factors affect vertebrate gut microbiome variation.

Geometrical description and Faddeev-Jackiw quantization of electrical networks

In lumped-element electrical circuit theory, the problem of solving Maxwell's equations in the presence of media is reduced to two sets of equations, the constitutive equations encapsulating local geometry and dynamics of a confined energy density, and the Kirchhoff equations enforcing conservation of charge and energy in a larger, topological, scale. We develop a new geometric and systematic description of the dynamics of general lumped-element electrical circuits as first order differential equations, derivable from a Lagrangian and a Rayleigh dissipation function. Through the Faddeev-Jackiw method we identify and classify the singularities that arise in the search for Hamiltonian descriptions of general networks. The core of our solution relies on the correct identification of the reduced manifold in which the circuit state is expressible, e.g., a mix of flux and charge degrees of freedom, including the presence of compact ones. We apply our fully programmable method to obtain (canonically quantizable) Hamiltonian descriptions of nonlinear and nonreciprocal circuits which would be cumbersome/singular if pure node-flux or loop-charge variables were used as a starting configuration space. We also propose a specific assignment of topology for the branch variables of energetic elements, that when used as input to the procedure gives results consistent with classical descriptions as well as with spectra of more involved quantum circuits. This work unifies diverse existent geometrical pictures of electrical network theory, and will prove useful, for instance, to automatize the computation of exact Hamiltonian descriptions of superconducting quantum chips.

Diversity, functional redundancy, and vulnerability of reef fish communities of the Saudi Arabian coast, in the northern Red Sea

In light of the current development of several giga-projects along the Saudi Red Sea coast, understanding the health status of coral reefs is paramount to developing baselines and management strategies to minimize the human impact on these ecosystems. Here, we present an assessment of the diversity, functional redundancy, and vulnerability for coral reef fish communities in the northern Red Sea. The focus area of our study falls within the marine jurisdiction of the NEOM giga-project. We performed fish surveys with three different methods aiming to cover a wide set of habitats and thus capture a comprehensive picture of the regional diversity: i) standardized belt transects, ii) roving diviersity surveys, and iii) collection of cryptobenthic fish. Our results showed a highly variable community composition along the ten surveyed sites highlighting Sindalah as an essential site for piscivore species, Al Muwaileh as an important site for herbivores, and Sanafir-Tiran as the most diverse site. Functional diversity analysis revealed similar levels of redundancy, over-redundancy, and vulnerability among sites. Functional richness, evenness, and divergence did not vary substantially between sites and, in general, indicated that the fish communities in the selected sites present mid to low levels of functional diversity. We further compare regional-scale and site-specific functional assessments. Notably, some of the species groups that are functionally redundant at the regional scale represented vulnerable functions at the site level. These results call to attention the need to create site-specific conservation strategies and not to solely rely on regional diviersity assessments as the basis for management decisions.

Current Status of Conservation and Exploitation of Unique Values of Typical Folk Art Forms in Tourism Development in Ethnic Minorities in Ha Giang Province, Vietnam

Ha Giang is a province in the northeastern region of Vietnam, adjacent to the border with China. The 21 ethnic groups living alternately created Ha Giang with a vibrant and diverse cultural picture. Implementing the strategy of taking traditional culture as a driving force for tourism development and promoting tourism to preserve culture, over the past time, Ha Giang province has been quite successful in bringing tangible and intangible cultural values to the world, in which there are types of folk art and literature into the model of community-based tourism to serve tourists. This article presents the current status of exploitation, conservation, and unique values of folk art and literature on ethnic minorities in the socio-economic development of Ha Giang province. This article also evaluates the achievements and limitations of several policies that have been implemented in the past. Hence, the solutions proposed to develop policies to exploit and preserve the values of natural resources include folk art and literature of the ethnic minorities in Ha Giang Province appropriately and effectively, contributing to the province's socio-economic development in the current context.

Formation of Vibration Fields for a Mechatronic Platform Driven by Dual Asynchronous Motors

This paper investigates the formation of vibration fields in a mechatronic setup driven by dual induction motors, relying on the controlled synchronization of unbalanced rotors. The proposed algorithm enables precise control over rotor speeds and phase shifts. Experimental results from a multi-resonance vibration laboratory setup demonstrate this approach’s ability to form the vibration fields. The ability to control these fields is crucial for applications such as vibratory transportation and the mixing of bulk materials. The results obtained can ensure a diverse picture of the complex trajectories of motion for various points of the platform, primarily in the screens, making various useful effects for vibration technologies. Additionally, the practical value of this research is that in the case of double synchronous mode the ordinate of the lower point of the trajectory is lower than in the case of single synchronous mode, which improves the efficiency of unloading and prevents congestion formation. The experimental data highlight the practical advantages and potential improvements in efficiency and reliability offered by this method.

Unroughing the cat’s tongue mushrooms: Four new species of Pseudohydnum from Brazil based on morphological and molecular phylogenetic evidence

ABSTRACT Pseudohydnum, commonly known as cat’s tongue mushrooms, is a monophyletic assemblage within Auriculariales, which encompasses species with gelatinous basidiomata, spathulate, flabellate, or shell-shaped pileus, hydnoid hymenophore, globose to ellipsoidal basidiospores, and longitudinally cruciate-septate basidia. According to the available literature, 16 species have been described in Pseudohydnum, mostly represented in temperate-boreal forests of the Northern Hemisphere. However, the limited morphological, molecular, and ecological information, especially from the Southern Hemisphere ecosystems, does not presently allow a reliable assessment of its taxonomic boundaries nor provide a complete picture of the species diversity in the genus. In an ongoing effort to examine specimens collected in dense and mixed ombrophilous forest fragments (Atlantic Rainforest domain) from Southeastern and Southern Brazil, additional taxa assigned to Pseudohydnum were identified. Four new species are recognized based mostly on characters of the pileus surface, stipe, hymenium, and basidiospores. Molecular phylogenetic analyses based on nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode), partial nuc rDNA 28S, and partial RNA polymerase II largest subunit (RPB1) sequences supported the description of these new taxa. Here, we propose Pseudohydnum brasiliense, P. brunneovelutinum, P. cupulisnymphae, and P. viridimontanum as new species. Morphological descriptions, line drawings, habitat photos, and comparisons with closely related taxa are provided. A dichotomous key for identification of currently known Southern Hemisphere Pseudohydnum species is presented.

Diverse Book Finders: Diversifying a Children’s Picture Book Collection by Integrating Acquisitions Into a Classroom Experience

Two recent trends in collection development include finding new ways to bring patron voices into collection development and developing strategies for increasing the diversity and representation within collections. Indiana University Southeast (IU-Southeast) Library recently developed a project that combined these two strategic directions by embedding an acquisition experience that used students enrolled in a sophomore block elementary education course to use the Diverse Book Finder (DBF) to select diverse picture books for the IU-Southeast collection. While diversification strategies and projects around children’s picture book collections in academic libraries are nothing new, IU-Southeast’sproject is unique in its incorporating of students to participate in the selection and diversification of a collection, rather than relying on more traditional strategies driven by librarian or faculty selection. This case study focuses on the design of the project, the integration and embedding of the acquisition project into the education course, how the DBF and its collection were leveraged to enhance the learning experience, and the effect this experience had on the diversity of the IU-Southeast Library collection and on the faculty, librarians, and students that were involved.

A Review of Genetic Diversity Based on the Y Chromosome in the Romanian Population.

Y chromosome analysis is used in a number of practical applications, including investigations of criminal cases, establishment of paternity, searching for missing persons, studies on human migration, evolutionary research, and historical and genealogical investigations. Questions about the origin of individual ethnic groups are addressed not only through archaeological, linguistic, and ethnographic methods but also through molecular genetics methods. The study of genetic diversity in Romania is particularly interesting from several perspectives because Romania, located in Southeast Europe, is distinguished by the fact that the Carpathians and the Danube served as natural barriers against the migrations of peoples for centuries, thus influencing the genetic mixture of the population. This is relevant for understanding the history and formation of ethnic groups in the region. In addition, many ethnic minorities live in Romania, which adds an additional dimension of genetic and cultural diversity. This article aims to provide an updated picture of the genetic diversity in Romania and to highlight the significant studies carried out among the Romanian population. By analyzing the articles published in the Web of Science, Scopus, or PubMed databases, which explore genetic diversity using the Y chromosome, the aim is to better understand the current genetic panorama in Romania.

Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

The plant adaptation response to a changing environment depends on the genetic diversity level it possesses. Genetic diversity and a thorough understanding of population indices are pivotal for decoding plant adaptation to dynamic environmental stressors. The development of polymerase chain reaction (PCR)-based molecular markers enables comprehensive population analyses and the precise detection of individuals and groups with unique genetic variations. Various molecular markers have been employed to assess genetic diversity, examine population structure, and delineate cluster patterns within and among populations. DNA markers revolutionize plant diversity studies by allowing detailed analyses of genetic variations, including economically significant trait-influencing genes. Despite their simplicity, they offer high reproducibility, ensuring accurate estimations of plant variation. Integrating multiple marker systems with advanced high-throughput sequencing techniques is poised to enhance the understanding and management of depleting plant genetic resources by providing a comprehensive picture of diversity at the genome-wide level. This review explores diverse molecular markers, elucidating their advantages and limitations, and highlights their impact on evaluating the genetic diversity and population structure of plants.

Exploring the diverse phenotypes of anti-GAD 65 encephalitis, diagnosis and treatment challenges

Objectives. To present two cases of anti-glutamic acid decarboxylase 65 (anti-GAD65) antibody encephalitis with different clinical phenotypes. Introduction. Anti-glutamic acid decarboxylase 65 antibody encephalitis (GAD-65 AE) is a rare pathology with a high potential for severity, having multiple forms of presentation and multiple differential diagnoses. This presentation aims to exemplify the strong clinical heterogeneity that is associated with this clinical entity by putting forth two cases that were treated in our clinic, each representing a separate clinical phenotype of this pathology. Material and methods. We analyzed two cases from our clinic of autoimmune encephalitis with positive anti-GAD65 antibodies and compared the cases with the data from the literature regarding clinical presentation, differential diagnosis, treatment, and evolution. Content. The first case presented is a woman aged 71 (66 at onset) presenting with progressive cerebellar ataxia. She was diagnosed with GAD-65 AE based on clinical presentation and positive anti-GAD65 antibodies titer. She was initially treated with methylprednisolone pulse therapy and received four such courses in the first year after diagnosis, resulting in a slowing of the progression of the neurological deficit. During the COVID19 pandemic, treatment was paused and her neurological status resumed its decline, and she also developed insulin-dependent diabetes mellitus. Starting in 2021, treatment was continued, having received one course of IVIg and one course of plasma exchange in our clinic. Eventually, the treatment was switched to Mycophenolate mofetil but her status continues to decline. The second case is about a 32 years old woman, with a history of recurrent seizures and progressive memory decline that started at 28 years. She was diagnosed 11 months after the onset with limbic encephalitis with anti-GAD65 antibodies, left temporal focal epilepsy with nocturnal seizures and mild cognitive decline. She was treated with corticotherapy and IgG. She was discharged home with antiepileptic treatment with no recurring seizures, but a slow cognitive decline. She had two relapses at distance from the onset, the first one with status epilepticus and the second one with focal seizures and a generalized seizure. She developed type I diabetes with positive anti-pancreatic islet antibodies. After the first relapse, immunosuppressive therapy with Mycophenolate mofetil was initiated. The evolution was good and under immunosuppression she had only one episode of seizures in the context of a COVID-19 infection. Conclusions. Autoimmune encephalitis with positive GAD antibodies presents a very diverse clinical picture, as we can see in the presented cases.

Embracing Complexity: What Novel Sequencing Methods Are Teaching Us About Herpesvirus Genomic Diversity.

The arrival of novel sequencing technologies throughout the past two decades has led to a paradigm shift in our understanding of herpesvirus genomic diversity. Previously, herpesviruses were seen as a family of DNA viruses with low genomic diversity. However, a growing body of evidence now suggests that herpesviruses exist as dynamic populations that possess standing variation and evolve at much faster rates than previously assumed. In this review, we explore how strategies such as deep sequencing, long-read sequencing, and haplotype reconstruction are allowing scientists to dissect the genomic composition of herpesvirus populations. We also discuss the challenges that need to be addressed before a detailed picture of herpesvirus diversity can emerge.

‘The uses of biography’: Life writing and geography

Geographers have regularly employed biography as a means to an end, that is, as a useful method for the investigation of some other phenomenon. But writing, reading, and collecting biographical memoirs has an inherent and not just an instrumental value. Linked to memory, biography becomes a way of recalling lives, including those not personally known to the reader, and remembering how one's own life and work is connected to those of predecessors and, by implication, those of successors. This article examines how biography can be used to foster an inclusive and diverse picture of the discipline which more fully appreciates the difficulties many geographers have overcome to pursue their geographical work, and the unnamed collaborators – colleagues, friends, and family – who supported them in their work. Portraiture is introduced as a complement to written memoir, as a subject which would bear further scrutiny.

The standardization of a new Explicit Pornographic Picture Set (EPPS)

Pictures with affective content have been extensively used in scientific studies of emotion and sexuality. However, only a few standardized picture sets have been developed that offer explicit images, with most lacking pornographic pictures depicting diverse sexual practices. This study aimed to fill this gap through developing a standardized affective set of diverse pornographic pictures (masturbation, oral sex, vaginal sex, anal sex, group sex, paraphilia) of same-sex and opposite-sex content, offering dimensional affective ratings of valence, arousal, and dominance, as well as co-elicited discrete emotions (disgust, moral and ethical acceptance). In total, 192 pornographic pictures acquired from online pornography platforms and 24 control IAPS images have been rated by 319 participants (Mage = 22.66, SDage = 4.66) with self-reported same- and opposite-sex sexual attraction. Stimuli were representative of the entire affective space, including positively and negatively perceived pictures. Participants showed differential affective perception of pornographic pictures according to gender and sexual attraction. Differences in affective ratings related to participants’ gender and sexual attraction, as well as stimuli content (depicted sexual practices and sexes). From the stimuli set, researchers can select explicit pornographic pictures based on the obtained affective ratings and technical parameters (i.e., pixel size, luminosity, color space, contrast, chromatic complexity, spatial frequency, entropy). The stimuli set may be considered a valid tool of diverse explicit pornographic pictures covering the affective space, in particular, for women and men with same- and opposite-sex sexual attraction. This new explicit pornographic picture set (EPPS) is available to the scientific community for non-commercial use.

A comprehensive study of prophage islands in Burkholderia pseudomallei complex

IntroductionBacteriophages are known as predators of bacteria and key biological factors influencing genetic recombination through phage transduction in bacteria. Phage transduction is known as one of the most common genetic recombination events found in Burkholderia pseudomallei, a diverse bacterial species and the causative agent of a deadly tropical disease melioidosis. The main objective of this study was to catalog prophages or prophage islands that are common in B. pseudomallei genomes.MethodsVarious bioinformatic tools were used to identify prophages in 106 complete B. pseudomallei genomes, and complete and incomplete genomes in other species within the B. pseudomallei Complex (BPC). Temperate phages were spontaneously induced from selected B. pseudomallei and B. thailandensis strains, and further characterized by transmission electron microscopy and whole genome sequencing.ResultsNine phage integration hotspots were identified in B. pseudomallei pan-genomes, eight of which were associated with tRNA gene-mediated site-specific recombination (tRNA-SSR) events. These genetic events occurred at various tRNA-genes including tRNA- Phenylalanine (anticodon GAA), - Methionine (CAU), - Proline (UGG), - Arginine (UCU), - Cysteine (GCA), - Arginine (CCG), - Serine (GGA), and – Selenocysteine (UCA) genes. Some of these events were also found in other related species within the B. pseudomallei Complex (BPC). We have demonstrated that lysogenic phages from select BPC strains could use B. pseudomallei strain Bp82 or 576mn as a host. These phages were classified into one of the two major groups, myoviruses or siphoviruses, based on their morphology and genomic composition.DiscussionWe have demonstrated that most B. pseudomallei strains are lysogenic, many containing at least one functional prophage in their genomes. Further investigation of the interactions between B. pseudomallei, bacteriophages, and other environmental and biological factors would provide a bigger picture of genomic diversity, potentially influence on survival of B. pseudomallei in the environment and its pathogenic specialization in hosts.

Mpox outbreak in Rio de Janeiro, Brazil: A translational approach.

Mpox is a zoonotic disease historically reported in Africa. Since 2003, limited outbreaks have occurred outside Africa. In 2022, the global spread of cases with sustained interhuman transmission and unusual disease features raised public health concerns. We explore the mpox outbreak in Rio de Janeiro (RJ) state, Brazil, in an observational study of mpox-suspected cases from June to December 2022. Data collection relied on a public healthcare notification form. Diagnosis was determined by MPXV-PCR. In 46 confirmed cases, anti-OPXV IgG was determined by ELISA, and seven MPXV genomes were sequenced. A total of 3095 cases were included, 816 (26.3%) with positive MPXV-PCR results. Most positive cases were men in their 30 s and MSM. A total of 285 (34.9%) MPXV-PCR+ patients live with HIV. Eight were coinfected with varicella-zoster virus. Anogenital lesions and adenomegaly were associated with the diagnosis of mpox. Females and individuals under 18 represented 9.4% and 5.4% of all confirmed cases, respectively, showing higher PCR cycle threshold (Ct) values and fewer anogenital lesions compared to adult men. Anti-OPXV IgG was detected in 29/46 (63.0%) patients. All analyzed sequences belonged to clade IIb. In RJ state, mpox presented a diverse clinical picture, represented mainly by mild cases with low complication rates and prominent genital involvement. The incidence in females and children was higher than usually reported. The observation of a bimodal distribution of Ct values, with few positive results, may suggest the need to review the diagnostic criteria in these groups.

Different trajectories for becoming bilingual lead to comparable outcomes in cognitive flexibility

Aims and Objective: Bilingualism has been proposed to affect cognitive flexibility, but findings in the literature are mixed. One reason for this might be the different trajectories of how participants acquired the second language, either through immersion or formal education. The present study investigates differences and commonalities of becoming bilingual depending on trajectory. Methodology: To assess cognitive flexibility, participants completed a task-switching and a language-switching paradigm, which were well-matched to each other. Language capabilities were assessed by self-report, objective fluency measures, and balance quotients. We assessed participants of five bilingual populations who together spoke five different languages. Data and Analysis: We analyzed global mixing costs, reflecting proactive language and task control, as well as local switch costs, reflecting reactive language and task control processes. The impact of language capabilities on mixing costs was assessed using linear mixed models. We also report correlations among language capability measures, sociodemographic variables, and working memory. Findings: We observed reliable mixing costs but no switch costs. Task and language mixing costs were smaller in participants who had a later age of L2 onset and higher L2 phonemic fluency, namely those who followed the formal education path to bilingualism. L2 phonemic fluency was the most consistent predictor for overall performance speed and reduced mixing costs. Originality: Using three different study sites and participants differing in their trajectory of becoming bilingual, this study provides a more diverse picture than many previous studies. Furthermore, using small language populations, the study shows the benefits of language fluency measures that are widely available. Significance and Implications: The present study bolsters the conceptualization of bilingualism as a multifaceted experience. Nonetheless, in the current study, bilingualism led to comparable outcomes in cognitive flexibility. Finally, measuring language capabilities should carefully consider cognitive processes not related to language that could explain variability in performance.

The potential of iNaturalist for bee conservation research—A study case in a southern Brazilian metropolis

Abstract Citizen science can expand our knowledge of biodiversity and enhance conservation programs by adding species records and associated data. This is the case for bees—the main group of pollinators—with millions of observations on iNaturalist. Here we ask if spontaneous observations made by citizen scientists on this platform provide a good picture of bee diversity in terms of taxonomic coverage (i.e., proportion of different subfamilies, tribes, genera and species), identification acuity (i.e., correct identification and at what taxonomic level) and taxonomic diversity. For this purpose, we compare iNaturalist observations with a dataset from a structured local survey for Curitiba, a city of 2 million inhabitants in southern Brazil. The search on iNaturalist resulted in 496 bee observations of 52 species, a richness similar to the most urbanised sampling site from the local survey. Highly eusocial and large bees are, proportionally, more frequently observed by citizens than sampled by professionals. Three citizen observers were responsible for more than 50% of the observations in iNaturalist. About 45% of species were correctly identified and this number increased up to 85% after our validation. We perceive that the spontaneous, non‐structured, observations on iNaturalist bring a biased representation of the bee fauna when compared to the structured local survey. However, the platform shows a great potential to monitor large bodied and eusocial bees, including rare and non‐native species; thus, it may be valuable for bee surveying, monitoring and conservation.

Refining the genomic profiles of North African sheep breeds through meta-analysis of worldwide genomic SNP data.

The development of reproducible tools for the rapid genotyping of thousands of genetic markers (SNPs) has promoted cross border collaboration in the study of sheep genetic diversity on a global scale. In this study, we collected a comprehensive dataset of 239 African and Eurasian sheep breeds genotyped at 37,638 filtered SNP markers, with the aim of understanding the genetic structure of 22 North African (NA) sheep breeds within a global context. We revealed asubstantial enrichment of the gene pool between the north and south shores of the Mediterranean Sea, which corroborates the importance of the maritime route in the history of livestock. The genetic structure of North African breeds mirrors the differential composition of genetic backgrounds following the breed history. Indeed, Maghrebin sheep stocks constitute a geographically and historically coherent unit with any breed-level genetic distinctness among them due to considerable gene flow. We detected a broad east-west pattern describing the most important trend in NA fat-tailed populations, exhibited by the genetic closeness of Egyptian and Libyan fat-tailed sheep to Middle Eastern breeds rather than Maghrebin ones. A Bayesian FST scan analysis revealed a set of genes with potentially key adaptive roles in lipid metabolism (BMP2, PDGFD VEGFA, TBX15, and WARS2), coat pigmentation (SOX10, PICK1, PDGFRA, MC1R, and MTIF) and horn morphology RXFP2) in Tunisian sheep. The local ancestry method detected a Merino signature in Tunisian Noire de Thibar sheep near the SULF1gene introgressed by Merino's European breeds. This study will contribute to the general picture of worldwide sheep genetic diversity.

Kelch 13 gene polymorphisms of Plasmodium falciparum among human population in Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Kelch-13 is a gene produced by Plasmodium falciparum, one of the species of Malaria parasite. Malaria remains a major public health challenge especially in Sub-Saharan Africa, a major cause of mortality and morbidity, especially in children and pregnant women. WHO recommends Artemisinin-based combination therapies as the first-line drugs for the treatment of uncomplicated malaria but the emergence and spread of Artemisinin-resistance associated with mutations in K13 gene poses a threat to ACT efficacy. Detection of mutant K13 gene may provide the information on changes in parasite susceptibility to Artemisinin. This study was aimed at detecting K13 gene polymorphisms among human population in Nasarawa-West Senatorial District, Nigeria. A total of 385 blood samples were collected from selected hospitals and screened for malaria by microscopy (the gold standard). Species specific screening of the P. falciparum was done using RDT. Dried blood spots made from RDT positive samples were investigated for the presence of K13 genes by nested PCR. Sequencing detected mutant K13 gene. Results: 103 samples were positive for Plasmodium falciparum by RDT, PCR confirmed 48 K13 genes with a band size of 848 bp. Nucleotide sequence alignment revealed six Single Nucleotides Polymorphisms. The nucleotide sequences were converted to protein sequences and results showed a point mutation in 1(5.0%) of the 20 Pfkelch 13 gene sequenced. Conclusively, the need for continuous surveillance following the detection of mutant gene in the study population is recommended in order to have a wider picture of the parasite diversity for effective malaria control.

Condyloma acuminata - the review of the treatment methods

Introduction and purpose:
 Condyloma acuminata, a benign manifestation of HPV infection, is a common sexually transmitted disease. Condyloma acuminata most commonly affects the anogenital area, but can also occur in the oral cavity. Macroscopically the lesions present as raised, fleshy papules, sometimes flat and broad, pedicled or cauliflower-like. Generally, the lesions are multiple, and as they grow, tend to coalesce into larger ones. Due to the lack of symptoms, condyloma acuminata are often found incidentally during routine medical appointments, e.g. in gynecologist’s offices. Additional symptoms and signs are pain, minor bleeding and pruritus. Patients suffering from condyloma acuminata are often in psychological and psychosexual distress. 
 This work aims to provide a summary of the currently available treatment options based on scientific reports to date.
 A brief description of the state of knowledge:
 Among the factors that increase the risk of the HPV infection are age, lifestyle, promiscuity, smoking, HIV infection, a history of chlamydia or gonorrhea infections. To date, no treatment algorithm has been developed for condyloma acuminata. Two vaccines are on offer as primary prevention, Gardasil and Cervarix. The available treatment options are divided into two main groups: patient-administered and physician administered. Surgical methods have the highest therapeutic efficacy. Condyloma acuminata has a high recurrence rate. Usually, a multi-session combination treatment is needed to reach total remission.
 Summary (conclusions):
 The treatment of condyloma acuminata includes personalized targets due to their diverse clinical picture and the limitations of certain patient groups. The available treatment options do not exhaust the needs of patients suffering from condyloma acuminata. Further research into the effectiveness of specific treatments is advisable.