Cadmium Phytoremediation Research Articles

Physiological response of Vetiveria Zizanioides to cadmium stress revealed by Fourier transform infrared spectroscopy

The present paper deals with the physiological response of the changes in chemical contents in the root, stem and leaf of Vetiveria zizanioides seedlings stressed by excess cadmium using Fourier transform infrared spectroscopy technique. Cadmium accumulation in plant and distribution in subcellular by atomic absorption spectroscopy were tested under different concentrations cadmium stress. The results show that low cadmium concentrations (<3 mg·L−1), the stem tissue of Vetiveria zizanioides can uses osmosis of organic substances (e.g., carbohydrates and amino acids) to improve cadmium tolerance. Organic acids secreted by the root of the plant could chelate cadmium, and the membrane lipid peroxidation of root, stem and leaf arise. It may be associated with cadmium tolerance threshold of Vetiveria zizanioides. In addition, the plant accumulated large amounts of cadmium, up to a maximum of 212.49 mg · kg−1(the shoot) and 290.59 mg · kg−1 dry weight (the root) on 10 days when the plant was treated with 7 mg·L−1 cadmium. The cadmium in the shoot (stem and leaf) usually increased with increasing cadmium concentration. Cadmium accumulated in leaves was mainly distributed in the cytoplasm, followed by the cell wall, and was least distributed in the mitochondria and chloroplasts. These studies demonstrated that the potential of Fourier transform infrared spectroscopy is the non-invasive and rapid technique for monitoring of the plants stressed with heavy metals, and Vetiveria zizanioides is suitable for phytoremediation of cadmium -contaminated soils.

Distribution of cadmium accumulated in the wood cells of poplar

Abstract Poplar is an important tree species in the phytoremediation of Cadmium (Cd) in the soil. However, the research on its transport and accumulation mechanism in wood cells has not been conducted. In this paper, Poplar 69 [Populus deltoides Bartr. cv. ‘Lux’ (I-69/55)] was selected to study the distribution of Cd accumulated in the wood, both macroscopically, by Cd analysis along the trunk heights, and microscopically, by scanning electron microscope with energy dispersion spectrum (SEM-EDS) observation, through a pot experiment, where four treatments with different Cd application in the soil and one control without Cd addition were set. The results demonstrated that the Cd concentration in the wood of all heights increased significantly with the increase of Cd addition in the soil and it also increased, though not significantly, from the basal to the top along the trunk heights at all treatments. Through SEM-EDS Cd was found to be accumulated in the inner wall and pits of the vessels, the wood fibers, and the wood rays, indicating that Cd could enter all three types of wood cells. The distribution of Cd in the three types of wood cells followed the order as wood fiber &gt; vessel &gt; wood ray, based on the calculation of the proportion of Cd enrichment spots in different types of cells. About half of the Cd in the wood was stored permanently in the wood fibers with the rest being in the vessels and wood rays probably temporally.

Arbuscular Mycorrhizal Fungi Are an Influential Factor in Improving the Phytoremediation of Arsenic, Cadmium, Lead, and Chromium.

The increasing expansion of mines, factories, and agricultural lands has caused many changes and pollution in soils and water of several parts of the world. In recent years, metal(loid)s are one of the most dangerous environmental pollutants, which directly and indirectly enters the food cycle of humans and animals, resulting in irreparable damage to their health and even causing their death. One of the most important missions of ecologists and environmental scientists is to find suitable solutions to reduce metal(loid)s pollution and prevent their spread and penetration in soil and groundwater. In recent years, phytoremediation was considered a cheap and effective solution to reducing metal(loid)s pollution in soil and water. Additionally, the effect of soil microorganisms on increasing phytoremediation was given special attention; therefore, this study attempted to investigate the role of arbuscular mycorrhizal fungus in the phytoremediation system and in reducing contamination by some metal(loid)s in order to put a straightforward path in front of other researchers.

Willow can be recommended as a strong candidate for the phytoremediation of cadmium and pyrene co-polluted soil under flooding condition.

Soil cadmium (Cd) and pyrene (PYR) pollutions have gained worldwide attention due to their negative effects on the environment. Intermittent flooding in rain-rich areas may affect phytoremediation of Cd and PYR in soil. Therefore, a pot-culture experiment, with and without flooding, was conducted to study the effects of flooding on soil Cd and PYR phytoremediation. Concentrations of Cd, PYR, and nutrients in soils and plants, as well as plant physiological and biochemical responses, were examined. Under both flooding and non-flooding conditions, willow (Salix × aureo-pendula CL 'J1011') demonstrated a better ability to remove soil Cd and PYR. Flooding led to higher Cd accumulation in roots than that in shoots. Conversely, non-flooding resulted in higher Cd accumulation in shoots than that in roots. The maximum concentrations of Cd in shoots were 11.02 and 14.07mgkg-1 with and without flooding, respectively. The maximum dissipation rates of PYR in soil were 47.35% and 88.61% under flooding and non-flooding conditions, respectively. In addition, flooding significantly increased the photosynthetic pigment, photosynthetic fluorescence, and chlorophyll fluorescence parameters in leaves, compared with non-flooding treatment. Flooding also increased the concentrations of Mg, Mn, P, Fe, and K in roots and shoots. This study outlines an effective insight for the phytoremediation of Cd- and PYR-contaminated soil under flooding condition.

Phytoremediation of cadmium contaminated alkaline soil using the ornamental hyperaccumulator Mirabilis jalapa L. enhanced by double harvesting: a field study.

Ornamental hyperaccumulators are considered ideal plants for phytoremediation of cadmium (Cd)-contaminated soils because of their high accumulation capacity and minimal potential to enter the food chain. Aiming to estimate the phytoremediation efficiency and explore the influence of double harvesting, a filed study was carried out in Cd-contaminated alkaline soil using the ornamental hyperaccumulator Mirabilis jalapa L. Seeds of M. jalapa were sawn with spacing in rows of 15cm and in lines of 20cm on April 20th. Three treatments with different harvesting strategies were arranged: all plants were harvested once on September 10th (marked with H0), and the plants were harvested on July 20th for the first time at a height above ground of 50cm and 30cm (marked with H1 and H2, respectively), and then, they were harvested on September 10th for the second time. The plant samples were digested with a mixture of HNO3 and HClO4 and determined using ICP-MS. The results showed that the total biomass of the shoots increased from 505.81 ± 8.29g/m2 in the H0 treatment to 849.72 ± 59.73 and 933.14 ± 96.12g/m2 in the H1 and H2 treatments, respectively, while the total Cd accumulation in the shoots was 42.32 ± 4.44, 52.99 ± 7.32, and 56.30 ± 6.95g/ha in the H0, H1, and H2 treatments, respectively. Phytoremediation efficiencies increased by at least 20% after the application of double harvesting. However, double harvesting also decreased the translocation and bioconcentration factors with a reduction of 30-55% because the Cd concentration in the shoots was low at the first harvest. Our results suggested that double harvesting is a powerful method to improve phytoremediation efficiency with low costs and minimal risk, and other technologies should be applied together to address the accumulation and translocation of Cd.

Tolerance mechanism and phytoremediation potential of Pistia stratiotes to zinc and cadmium co-contamination

Pistia stratiotes can not only effectively remediate eutrophic water, but also displays strong absorption and bioaccumulation abilities for heavy metals. However, it has not been well-understood how the plant resists the combined stress of heavy metals. In these experiments, the morphophysiological traits, the ascorbate-glutathione (AsA-GSH) cycle, the glyoxalase system, and the contents of zinc (Zn) and cadmium (Cd) were investigated under Zn and Cd co-pollution. The AsA-GSH cycle and glyoxalase system could coordinately alleviate the oxidative and carbonyl stress, which was identified as an important tolerance mechanism. With Zn50Cd1, Zn50Cd10, Zn100Cd1, and Zn100Cd10 treatments for 18 days, 90.75–93.69% of Zn and 88.13–96.96% Cd accumulated in the roots. Treatments with Zn50Cd50, and Zn100Cd50 for 18 days resulted in a decrease of stress tolerance and chlorophyll content in leaves, an increase in plasma membrane permeability, a massive accumulation of methylglyoxal (MG), and visible toxic symptoms. Additionally, the bioaccumulation factor (BCF) for roots and shoots and the translocation factor (TF) were >1, and the content of Cd in shoots was no <100 mg·kg−1. This indicated P. stratiotes was a Cd hyperaccumulator and have great potential for the phytoremediation of heavy metal contaminated water. Novelty statement Pistia stratiotes, a cadmium hyperaccumulator, has great application potential for the phytoremediation of zinc and cadmium co-polluted water.

Phytoremediation of Cadmium Polluted Soils: Current Status and Approaches for Enhancing

Cadmium (Cd) is a heavy metal present in atmosphere, rocks, sediments, and soils without a known role in plants. It is relatively mobile and can easily enter from soil into groundwater and contaminate the food chain. Its presence in food in excess amounts may cause severe conditions in humans, therefore prevention of cadmium entering the food chain and its removal from contaminated soils are important steps in preserving public health. In the last several years, several approaches for Cd remediation have been proposed, such as the use of soil amendments or biological systems for reduction of Cd contamination. One of the approaches is phytoremediation, which involves the use of plants for soil clean-up. In this review we summarized current data on the use of different plants in phytoremediation of Cd as well as information about different approaches which have been used to enhance phytoremediation. This includes data on the increasing metal bioavailability in the soil, plant biomass, and plant accumulation capacity as well as seed priming as a promising novel approach for phytoremediation enhancing.

Mutualistic fungus Piriformospora indica modulates cadmium phytoremediation properties of host plant via concerted action of enzymatic and non-enzymatic biochemicals

Soils and ecosystems contaminated with cadmium (Cd) threaten human health and adversely affect morphological, physiological, and biochemical parameters of plants. The symbiotic association of endophytic fungi with their host plants is the best strategy to improve various plant characteristics and remediate soils polluted with heavy metal(loid)s (HMs). Being a well-known plant growth-promoting fungus, Piriformospora indica confers resistance against a number of abiotic stresses, including HM stress. This pot experiment explored the potential and ameliorative effects of P. indica on Artemisia annua L. plants treated with different concentrations (0, 40, 80, and 120 mg kg -1 ) of Cd. Inoculation with P. indica significantly increased plant performance, especially by enhancing chlorophyll concentration and water potential and by decreasing electrolytic leakage, when compared with un-inoculated plants, despite the high Cd levels. Similarly, P. indica enhanced antioxidant enzyme activities, thereby reducing the drastic effects of Cd in inoculated plants. In addition, P. indica accumulated Cd in the roots of colonized plants, as revealed by atomic absorption spectroscopy, and restricted Cd translocation to aerial parts. Furthermore, P. indica showed in vitro resistance (up to a certain level) to Cd stress; however, fungus growth was inhibited at very high Cd concentrations, proving it an excellent candidate for use as a potential phytoremediator in fields affected by Cd contamination. The transcriptional analysis showed that the signaling genes and artemisinin and flavonoid biosynthetic pathway genes were significantly upregulated in P. indica -co-cultivated plants when compared with un-inoculated plants, suggesting a fine collaboration between primary and secondary metabolisms to modulate resistance capacity and to enhance the phytoremediation capability of A. annua against Cd toxicity.

Cadmium binding during leaf senescence in Festuca arundinacea: Promotion phytoextraction efficiency by harvesting dead leaves

Phytoextraction by harvesting dead leaves is a novel cadmium (Cd) phytoremediation strategy in tall fescue (Festuca arundinacea), which provides feasibility for the phytoremediation of Cd-polluted soils and cleaner food production. The highest Cd in dead leaves is the result of Cd accumulation during the process of leaf senescence. However, it is not known the mechanism of Cd accumulation during the leaf senescence, which limits the phytoextraction efficiency of this technology. In this study, we found that the contents of phytochelatins (PC), glutathione (GSH), and non-protein thiols (NPT) were increased during the process of leaf senescence and Cd stress significantly promoted PC, GSH, and NPT. Transcriptome analysis showed that the pathway of glutathione metabolism was significantly enriched in the senescent leaf under Cd stress. 19 genes encoding GST, enzymes catalyzing GSH-Cd binding, were up-regulated in the senescent leaf. The increases of PC, GSH, and NPT in the senescent leaf for Cd-binding could be from the pathways of the protein degradation rather than their synthesis, because genes encoding cysteine protease (catalyzes protein degradation) were significantly promoted, but both GSH synthetase (GS) and PC synthetase (PCS) did not show the significant changes between the young and senescent leaves. Our results indicated that Cd accumulation during the leaf senescence could be the result of the promotion of Cd-binding by PC, GSH, and NPT, which provide insights into the regulatory mechanism and further genetic engineering to promote the phytoextraction efficiency by harvesting dead leaves in tall fescue.

Phytoremediation of Cadmium Contaminated Soil Using Sesbania sesban L. in Association with Bacillus anthracis PM21: A Biochemical Analysis

Sustainable food production to feed nine to 10 billion people by 2050 is one of the greatest challenges we face in the 21st century. Due to anthropogenic activities, cadmium (Cd) contamination is ubiquitous with deleterious effects on plant and soil microbiota. In the current study, the phytoremediation potential of Sesbania sesban L. was investigated in Cd-spiked soil inoculated with Bacillus anthracis PM21. The Cd-spiked soil drastically reduced important plant attributes; however, inoculation of B. anthracis PM21 significantly (p ≤ 0.05) enhanced root length (17.21%), shoot length (15.35%), fresh weight (37.02%), dry weight (28.37%), chlorophyll a (52.79%), chlorophyll b (48.38%), and total chlorophyll contents (17.65%) at the Cd stress level of 200 mg/kg as compared to the respective control. In addition, bacterial inoculation improved superoxide dismutase (11.98%), peroxidase (12.16%), catalase (25.26%), and relative water content (16.66%) whereas it reduced proline content (16.37%), malondialdehyde content (12.67%), and electrolyte leakage (12.5%). Inoculated plants showed significantly (p ≤ 0.05) higher Cd concentration in the S. sesban root (118.6 mg/kg) and shoot (73.4 mg/kg) with a translocation (0.61) and bioconcentration factor (0.36), at 200 mg/kg Cd. Surface characterization of bacteria through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) predicted the involvement of various functional groups and cell surface morphology in the adsorption of Cd ions. Amplification of the CzcD gene in strain PM21, improved antioxidant activities, and the membrane stability of inoculated S. sesban plants conferred Cd tolerance of strain PM21. In addition, the evaluated bacterial strain B. anthracis PM21 revealed significant plant growth-promoting potential in S. sesban; thus, it can be an effective candidate for phyto-remediation of Cd-polluted soil.

Biochelator Assisted Phytoremediation for Cadmium (Cd) Pollution in Paddy Field

To evaluate the efficacy of biochelators for phytoremediation of cadmium (Cd), effects of biochelators (humic acid, HA; polyaspartic acid, PASP; rhamnolipid, RLs) and synthetic chelator (sodium tripolyphosphate, STPP) on the growth, accumulation, and antioxidant index of Cd were investigated in Iris sibirica L. The results indicated that the addition of HA increased the dry weight of leaves and stems, and the addition of PASP, RLs, and STPP increased the growth of leaves but inhibited the growth of stems. The determination of Cd accumulation indicated that HA decreased the content of Cd in all tissues, while the addition of PASP, RLs, and STPP increased the accumulation of Cd in stems and roots. In addition, the determination of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities indicated that the treatment of PASP and RLs increased the activities of antioxidant enzymes, which might be beneficial to the resistance of Cd for Iris sibirica L. Overall, these results indicated that biochelators may improve the phytoremediation efficacy of Iris sibirica L. on Cd polluted paddy field. Among the tested chelators, PASP exhibited the best comprehensive efficacy and the highest commercial potential.

The Road to Practical Application of Cadmium Phytoremediation Using Rice

Cadmium (Cd) is a toxic heavy metal that causes severe health issues in humans. Cd accumulates in the human body when foods produced in Cd-contaminated fields are eaten. Therefore, soil remediation of contaminated fields is necessary to provide safe foods. Rice is one of the primary candidates for phytoremediation. There is a genotypic variation of Cd concentration in the shoots and grains of rice. Using the world rice core collection, ‘Jarjan’, ‘Anjana Dhan’, and ‘Cho-ko-koku’ were observed with a significantly higher level of Cd accumulation in the shoots and grains. Moreover, OsHMA3, a heavy metal transporter, was identified as a responsive gene of quantitative trait locus (QTL) for high Cd concentration in the shoots of these three varieties likewise. However, it is difficult to apply practical phytoremediation to these varieties because of their unfavorable agricultural traits, such as shatter and easily lodged. New rice varieties and lines were bred for Cd phytoremediation using OsHMA3 as a DNA marker selection. All of them accumulated Cd in the shoots equal to or higher than ‘Cho-ko-koku’ with improved cultivation traits. Therefore, they can be used for practical Cd phytoremediation.

Assessment of the potential for phytoremediation of cadmium polluted soils by various crop rotation patterns based on the annual input and output fluxes

Phytoremediation potential of two oil crop rotations (oilseed sunflower–rape (O+Ra) and peanut–oilseed rape (P+Ra)) was compared with three conventional cropping patterns (rice–rape (R+Ra), rice–rice (R+R), single cropped rice (SR)) in experimental plots with cadmium (Cd)-contaminated soil. A new approach was used to evaluate phytoremediation potential based on the balance between annual input and output fluxes of Cd in farmland soil. In O+Ra and P+Ra rotations, 77.24 and 62.09 g/ha Cd were removed, respectively, whereas in R+Ra, R+R, and SR patterns, 41.79, 46.46, and 23.85 g/ha Cd were removed, respectively. The balance between inputs and outputs of Cd was − 40.72 and − 25.76 g/ha under O+Ra and P+Ra rotations, respectively. Available Cd content in topsoil was reduced by 5.58% and 3.91% under O+Ra and P+Ra rotations, respectively. Based on the balance between Cd inputs and outputs, phytoremediation efficiencies of O+Ra (1.23%) and P+Ra (0.78%) rotations were higher than those of R+R (0.29%), R+Ra (0.13%), and SR (−0.38%) systems. Because crop removal is the main Cd output pathway, selection of a suitable crop is particularly important in remediation of Cd-contaminated farmland.

Effect of the natural establishment of two plant species on microbial activity, on the composition of the fungal community, and on the mitigation of potentially toxic elements in an abandoned mine tailing

In Mexico, millions of tons of mining wastes are deposited in the open pit. Their content in potentially toxic elements (PTE) represents an environmental risk. In the tailings, pioneer plant communities are established, associated with a determined diversity of fungi; plants, and fungi are fundamental in the natural rehabilitation of mining wastes. The objective was to evaluate the impact of the natural establishment of two plant species on the microbial activity, on the composition of the fungal community, and on the mitigation of the effect of PTE in a contaminated mine tailing. In a tailing, we selected three sites: one non-vegetated; one vegetated by Reseda luteola, and one vegetated by Asphodelus fistulosus. In the substrates, we conducted a physical and chemical characterization; we evaluated the enzymatic activity, the mineralization of the carbon, and the concentration of PTE. We also determined the fungal diversity in the substrates and in the interior of the roots, and estimated the accumulation of carbon, nitrogen, phosphorus and PTE in plant tissues. The tailings had a high percentage of sand; the non-vegetated site presented the highest electric conductivity, and the plant cover reduced the concentration of PTE in the substrates. Plants increased the carbon content in tailings. The enzymatic activities of β-glucosidase and dehydrogenase, and the mineralization of carbon were highest at the site vegetated with A. fistulosus. Both plant species accumulated PTE in their tissues and exhibited potential in the phytoremediation of lead (Pb), cadmium (Cd), and copper (Cu). Fungal diversity was more elevated at the vegetated sites than in the bare substrate. Ascomycota prevailed in the substrates; the substrates and the plants shared some fungal taxa, but other taxa were specific. The plant coverage and the rhizosphere promoted the natural attenuation and a rehabilitation of the extreme conditions of the mining wastes, modulated by the plant species.

Integrated ionomic and transcriptomic dissection reveals the core transporter genes responsive to varying cadmium abundances in allotetraploid rapeseed

BackgroundOilseed rape (B. napus L.) has great potential for phytoremediation of cadmium (Cd)-polluted soils due to its large plant biomass production and strong metal accumulation. Soil properties and the presence of other soluble compounds or ions, cause a heterogeneous distribution of Cd.ResultsThe aim of our study was to reveal the differential responses of B. napus to different Cd abundances. Herein, we found that high Cd (50 μM) severely inhibited the growth of B. napus, which was not repressed by low Cd (0.50 μM) under hydroponic culture system. ICP-MS assays showed that the Cd2+ concentrations in both shoots and roots under 50 μM Cd were over 10 times higher than those under 0.50 μM Cd. Under low Cd, the concentrations of only shoot Ca2+/Mn2+ and root Mn2+ were obviously changed (both reduced); under high Cd, the concentrations of most cations assayed were significantly altered in both shoots and roots except root Ca2+ and Mg2+. High-throughput transcriptomic profiling revealed a total of 18,021 and 1408 differentially expressed genes under high Cd and low Cd conditions, respectively. The biological categories related to the biosynthesis of plant cell wall components and response to external stimulus were over-accumulated under low Cd, whereas the terms involving photosynthesis, nitrogen transport and response, and cellular metal ion homeostasis were highly enriched under high Cd. Differential expression of the transporters responsible for Cd uptake (NRAMPs), transport (IRTs and ZIPs), sequestration (HMAs, ABCs, and CAXs), and detoxification (MTPs, PCR, MTs, and PCSs), and some other essential nutrient transporters were investigated, and gene co-expression network analysis revealed the core members of these Cd transporters. Some Cd transporter genes, especially NRAMPs and IRTs, showed opposite responsive patterns between high Cd and low Cd conditions.ConclusionsOur findings would enrich our understanding of the interaction between essential nutrients and Cd, and might also provide suitable gene resources and important implications for the genetic improvement of plant Cd accumulation and resistance through molecular engineering of these core genes under varying Cd abundances in soils.

Cadmium phytoremediation potential of Deenanath grass (Pennisetum pedicellatum) and the assessment of bacterial communities in the rhizospheric soil.

Phytoremediation technology is gaining excessive consideration as a promising method for heavy metal remediation from contaminated soil. In the present research study, a greenhouse trial was performed to assess the proficiency of Pennisetum pedicellatum as a potential plant species for the remediation of cadmium from the soil. Four sets of treatments i.e., (To) control, (T1) 25 ppm, (T2) 50 ppm, and (T3) 100 ppm were studied till 60 days. Soil and plant samples were collected at a regular interval of 15 days after the seed sowing and analysed for different physicochemical properties and Cd concentrations from each treatment. The cadmium uptake was studied in the roots and shoots independently to examine the cadmium accumulation in P. pedicellatum. The present study showed that P. pedicellatum accumulated cadmium mostly in their roots compared to the shoots resulting in the accumulation of Cd from the soil. The finding indicates that P. pedicellatum is a virtuous plant species to restore cadmium-contaminated soil. It effectively banished 83% of Cd from the 100 ppm spiked soil at the end of 60 days. The microbial characterization of rhizospheric soil was also done using serial dilution and spread plate procedures to determine the presence of bacterial species in the rhizospheric soil. Seven bacterial strains were isolated from the soil and were further assessed for their biochemical, molecular, and phylogenic characteristics. The 16S rRNA sequencing analysis confirmed the presence of different bacterial species such as Alcaligenes sp., Bacillus drentensis, Bacillus subtilis, Bacillus foraminis, Bacillus wudalianchiensis, Bacillus amyloliquefaciens, and Planococcus ruber. This study concluded that phytoremediation using P. pedicellatum is a fascinating and compelling green technology for the remediation of cadmium from soil.

Phytoremediation of cadmium-contaminated sediment using Hydrilla verticillata and Elodea canadensis harbor two same keystone rhizobacteria Pedosphaeraceae and Parasegetibacter

Aquatic macrophytes have been widely employed for in-situ phytoremediation of cadmium (Cd) polluted sediments. But, little is known about the responses of rhizosphere bacteria and their interspecific interactions to phytoremediation. In this study, the α-diversity, community composition, co-occurrence network and keystone species of sediment bacteria in rhizosphere zones of two typical macrophytes, Hydrilla verticillata and Elodea canadensis, were investigated using 16S rRNA gene high-throughput sequencing. The results showed that after fifty days of phytoremediation, a group of specialized sediment bacteria were assembled in the rhizosphere zones closely associated with different host macrophytes. Rhizosphere micro-environments, i.e., the increases of redox potential and organic matter and the decreases of pH, nitrogen and phosphorus, reduced bacterial α-diversity through niche-based species-sorting process, which in turn reduced interspecific mutualistic relationships. But meanwhile, benefiting from the nutrients supplied from macrophyte roots, more bacterial species survived in the highly Cd-contaminated sediments (50 mg kg−1). In addition, the co-occurrence network revealed that both macrophytes harbored two same keystone bacteria with the high betweenness centrality values, including the family Pedosphaeraceae (genus_unclassified) and genus Parasegetibacter. Their relative abundances were up to 28-fold and 25-fold higher than other keystone species, respectively. Furthermore, these two keystone bacteria were metabolic generalists with vital ecological functions, which posed significant potentials for promoting plant growth and tolerating Cd bio-toxicity. Therefore, the identified keystone rhizobacteria, Pedosphaeraceae and Parasegetibacter, would be potential microbial modulations applied for the future optimization of phytoremediation in Cd-contaminated sediment.

Metabolic Profiles of Brassica juncea Roots in Response to Cadmium Stress.

Brassica juncea has great application potential in phytoremediation of cadmium (Cd)-contaminated soil because of its excellent Cd accumulating and high biomass. In this study, we compared the effects of Cd under 48 h and 7 d stress in roots of Brassica juncea using metabolite profiling. The results showed that many metabolic pathways and metabolites in Brassica juncea roots were altered significantly in response to Cd stress. We found that significant differences in levels of amino acids, organic acids, carbohydrates, lipids, flavonoids, alkaloids, and indoles were induced by Cd stress at different times, which played a pivotal role in the adaptation of Brassica juncea roots to Cd stress. Meanwhile, Brassica juncea roots could resist 48 h Cd stress by regulating the biosynthesis of amino acids, linoleic acid metabolism, aminoacyl-tRNA biosynthesis, glycerophospholipid metabolism, ABC transporters, arginine biosynthesis, valine, leucine and isoleucine biosynthesis, and alpha-linolenic acid metabolism; however, they regulated alpha-linolenic acid metabolism, glycerophospholipid metabolism, ABC transporters, and linoleic acid metabolism to resist 7 d Cd stress. A metabolomic expedition to the response of Brassica juncea to Cd stress will help to comprehend its tolerance and accumulation mechanisms of Cd.

Phytoremediation of cadmium contaminated soils by Amaranthus Hypochondriacus L.: The effects of soil properties highlighting cation exchange capacity

Phytoremediation is a cost-effective method to remedy Cd-contaminated soils. However, it is difficult to predict the performance of a given (hyper)accumulator at different soils due to the divergent plant-soil mutual fitness. Soil properties could be quite influential in determining plant growth and Cd uptake and therefore affect phytoremediation efficiency. To explore the primary soil factors that regulate the efficiency of phytoremediation, a phytoextraction experiment with grain amaranth (Amaranthus Hypochondriacus L.) was conducted in six long-term Cd-contaminated agricultural soils from southern China. The results showed that besides the soil available Cd, the soil cation exchange capacity (CEC) greatly affected plant growth and the amount of total Cd extraction. The deficiency of available Ca and Mg in low CEC soil caused insufficient uptake of Ca and Mg by grain amaranth, which was adverse to plant growth and Cd detoxification. The impaired plant biomass production sharply influenced plant total Cd accumulation, despite the relatively high Cd concentration in plants. While for the grain amaranth grown in soils with higher CEC, the increases in plant Ca and Mg promoted plant photosynthesis and plant tolerance to Cd stress, as indicated by the increase of leaf chlorophyll content and antioxidant enzyme activities, which contributed to the higher plant biomass and phytoremediation efficiency. These findings highlight that maintaining regular plant biomass production is vital to ensure the efficiency of phytoremediation, and low CEC of soil is a substantial barrier that needs to be concerned and further addressed for efficient phytoremediation of Cd-contaminated soils.

Sargassum, Padina and Turbinaria as bioindicators of cadmium in Bais Bay, Negros Oriental

Marine pollution is becoming one of the global environmental problems around the world. Heavy metal concentrations in the environment is 100-1,000 folds higher than those in the Earth’s crust due to anthropogenic sources. The use of algae as bioindicators of metal pollution and the effective removal of toxic metal ions has received increasing attention. Brown algae is thought to be excellent in the sequestration of metal. In this study, cadmium (Cd) concentration in the thalli of Sargassum sp., Padina sp. and Turbinaria sp. from North Bais Bay, Negros Oriental was determined using Atomic Absorption Spectrophotometer (AAS). The concentration of cadmium in the sediment of North Bais Bay was also determined because environmental pollutants could also accumulate in the sediment. Results of this study showed the cadmium contamination in Bais Bay, wherein Sargassum sp., Padina sp., and Turbinaria sp. absorbed these cadmium ions. Sargassum sp. had the highest concentration of cadmium which ranged from 2.14 to 4.45 mg kg-1 . The concentration of cadmium in Padina sp. and Turbinaria sp. ranged from 2.2 to 3.4 mg kg-1 and 2.36 to 2.76 mg kg-1 , respectively. The concentration of cadmium in the sediment ranged from 3.72 to 5.53 mg kg-1 dry weight. This indicates that these brown algal species could be utilized as bioindicators of cadmium contamination in marine waters and possible phytoremediation of cadmium in wastewater.