Phytochemical Research Articles

C(sp3)-H Carbonylative Cyclization of Hydrazones with CO2: Synthesis of Pyrazolone Derivatives.

A novel method is reported to synthesize various pyrazolones through transition-metal-free and redox-neutral 1°, 2°, or 3° C(sp3)-H carbonylative cyclization using 1 atm of CO2 as a green carbonyl source, featuring good functional group tolerance, a broad substrate scope, facile scalability, and easy product transformation. The utility of this method could be demonstrated by the applications in preparing useful synthetic intermediates and bioactive compounds.

Phyto-nutraceutical promise of Brassica vegetables in post-genomic era: a comprehensive review.

Brassica vegetables are one of the possible solutions to tackle the emerging human diseases and malnutrition due to their rich content of phyto-nutraceutaical compounds. The genomics enabled tools have facilitated the elucidation of molecular regulation, mapping of genes/QTLs governing nutraceutical compounds, and development of nutrient-rich Brassica vegetables. The enriched food products or foods as whole termed as functional foods are intended to provide health benefits. The 2500year old Hippocratic phrase 'let thy food be thy medicine and thy medicine be thy food' remained in anonymity due to lack of sufficient evidence. However, today, we are facing reappraisal of healthy nutritious functional foods in battling diseases. In this context, the Brassica vegetables represent the most extensively investigated class of functional foods. An optimal consumption of Brassica vegetables is associated with lowering the risks of several types of cancer, chronic diseases, cardiovascular disease, and help in autism. In the post-genomic era, the integration of genetic and neoteric omics tools like transcriptomics, metabolomics, and proteomics have illuminated the downstream genetic mechanisms governing functional food value of Brassica vegetables. In this review, we have summarized in brief the phyto-nutraceutical profile and their functionality in Brassica vegetables. This review also highlights the progress made in identification of candidate genes/QTLs for accumulation of bioactive compounds in Brassica vegetables. We summarize the molecular regulation of major phytochemicals and breeding triumphs in delivering multifunctional Brassica vegetables.

The Multifaceted Applications of Seaweed and Its Derived Compounds in Biomedicine and Nutraceuticals: A Promising Resource for Future.

The increasing demand for global food resources and over-dependence on terrestrial agroecosystems pose a significant challenge to the sustainable production of food commodities. Macroalgae are an essential source of food production in the marine environment, and their cultivation is a promising approach to alleviate the impending global food insecurity due to key factors, such as independence from terrestrial agriculture, rapid growth rate, unique biochemical composition, and carbon capture potential. Moreover, in many countries, seaweed has been used as food for decades because of its health and nutritional benefits. Seaweed contains bioactive components that are beneficial against various pathological conditions, including cancer, type 2 diabetes, and neurological disorders. Furthermore, the natural products derived from macroalgae have also been found to have immunostimulatory and antimicrobial properties. Macroalgae are also a significant source of rare sugars such as L-fucose, L-rhamnose, and glucuronic acid. Besides sugars, other bioactive components have been widely reported for their potential in cosmeceuticals. We have outlined the nutrient composition and functional properties of different species of macroalgae, with an emphasis on their potential as value-added products to the functional food market. Beyond being nutritional powerhouses, the variety of biological activities in human health and biomedicine makes them excellent candidates for developing novel drugs. Therefore, this review summarizes the pharmaceutical applications of macroalgae and suggests potential strategies for incorporating macroalgae-derived bioactive compounds into therapeutic products.

Electrochemical Dehydrogenative sp2-Coupling Reaction of Naphthols Accessing a Polycyclic Naphthalenone Motif.

A novel polycyclic naphthalenone motif was obtained by electrochemical synthesis starting from naphthols. The process is solvent controlled, and the highly diastereoselective cyclization is due to a solvent cage. The direct, anodic dehydrogenative sp2-coupling was carried out by flow electrolysis. Ten derivatives containing this motif were synthesized in yields up to 88%, resulting in novel polycycles structurally similar to bioactive compounds like Daldionin, potentially exploring the bioactive profile.

Access to 2,3-Dihydropyridines Involving Cyano Transformation Relay through Gold Catalysis Assisted by a Lewis Acid.

A gold(I)-catalyzed cyclization of enynone-nitriles with amines to 2,3-DHPs assisted by La(OTf)3 has been developed. The compatibility with abundant nucleophiles, high functional group tolerance, rapid assembly of molecular complexity, and late-stage functionalization of bioactive substances make this approach attractive for the construction of 2,3-DHPs, which have rarely been synthesized in the literature. The reaction proceeds via cascade cyclization involving gold/Lewis acid cooperative activation of the alkene and the cyano moiety.

Nutritional and Bioactive Compounds, Antioxidant Properties and Antibacterial Activity of Chinese Chive (Allium odorum L.).

The present study was conducted to generate the comprehensive data on the nutritional composition, minerals, heavy metal, bioactive compounds, antioxidant and antibacterial properties of Chinese chive (Allium odorum L.) grown at two different locations including Manipur and Punjab. The crop grown in Manipur exhibited higher values of nutritional and phytochemical compound values than those grown in Punjab. It exhibited significantly higher amounts of crude protein, crude fiber, potassium, calcium and iron content in the Manipur environment. The allicin content in leaves of Chinese chive on a dry weight (dw) varied from 1.31 to 1.4% and there was non-significant difference in allicin content at both locations. Besides, the heavy metal content of Chinese chive was much below the permissible limits of the World Health Organization. The IC50 value of Chinese chive was 78.908-95.140µg/ml which imparts strong antioxidant activity and higher DPPH (2,2-diphenyl-1-picrylhydrazyl)radical scavenging activity higher in fresh leaves than dried leaves. Total phenol and total flavonoid were found to be significantly high. In addition, high antibacterial activities were observed against toxin producing pathogenic bacterial strains of Staphylococcus aureus and Enterococcus faecalis. The nutritional and bioactive compounds, antioxidant properties and antibacterial activity of Chinese chive make it ideal for its use as a functional food and/or supplement.

Medicinal Plants as A Natural Immunity Booster Against Covid 19: A Review

Medicinal plants have long been valued for their therapeutic properties, especially in boosting immune function. This review explores the role of medicinal plants in enhancing immunity during the COVID-19 pandemic, with an emphasis on plants known for their bioactive compounds and health benefits. A thorough search of online databases resulted in the identification of 115 published articles from 2020 to 2024, which led to the selection of 50 plants commonly used for immune support. Plants include Garlic, Ashwagandha, Neem,Turmeric, Guduchi, Holy Basil, Amla, Aloe Vera, Black Cardamom, Cinnamon, Pomegranate, Guava, Moringa, Black Cumin, Lemon, Lemongrass, Papaya, Green Tea and others. These plants contain bioactive compounds such as flavonoids, alkaloids, and terpenoids, which are believed to possess immune-boosting, anti-inflammatory, and antiviral properties. Garlic, Turmeric, and Ashwagandha are particularly noted for combating oxidative stress and supporting immune function, while Gurjo, Neem, and Amla are valued for their detoxifying and anti-inflammatory effects. These plants, traditionally used during viral infections like the flu and COVID-19, represent a fusion of ancient cultural wisdom and modern scientific research.Future research should focus on clinical validation and pharmacological studies to better understand the efficacy of these plants in immune modulation.

Upcycled cosmetic ingredients: extraction process optimization via green solvents by the means of I-optimal and Box–Behnken designs

Current trends are shifting attention to the sustainable valorization of food bio-waste management and by-product utilization, as they are rich in nutrients and bioactive compounds, such as dietary fiber, polyphenols, and peptides. Upcycled ingredients and green cosmetics are becoming more and more appealing concepts in the nutraceutical and cosmetic industries encouraging them to invest and develop sustainable products aligning with the green concept of circular economy. In this research, we focused on the waste products (peels) of super exotic fruits Litchi chinensis and Selenicereus undatus, which are packed with antioxidants, fibers, vitamins, and minerals. Extraction of the peels with green solvent (propylene glycol) was performed and optimized by the means of an I-optimal and box-behnken designs with different independent variables for each design and total phenolic content (TPC) as a response. The resulted optimized extract of lychee peels propylene glycol extract (LPPG) was evaluated in terms of anti-tyrosinase activity, resulting in an (IC50 = 264.1 ± 16.9 µg/mL) with an average inhibition percentage of (92.33%), DPPH assay was performed, revealing antioxidant activity of (2666.7 ± 68.71 µmol ascorbic acid/mL) and (24,774.68 ± 834.67 µmol ascorbic acid/mL) using ABTS assay. As for dried dragonfruit propylene glycol extract (DDPG), the antioxidant activity was found to be (1299.82 ± 20.80 µmol Ascorbic acid/mL) using DPPH assay and (1510.506 ± 12.93 µmol Ascorbic acid/mL) using ABTS assay. In conclusion, optimization of the extraction process of food by-products using propylene glycol as a solvent may offer a valuable sustainable cosmetic ingredient.

Synthesis of 3-Substituted Indoles by Yonemitsu Three-Component Reactions Accelerated in Microdroplet/Thin Film.

3-Substituted indoles are an important framework of many drugs, agricultural chemicals, functional materials, and bioactive compounds. Malononitrile-based three-component Yonemitsu reactions are attractive choices for the synthesis of 3-substituted indole derivatives but suffered from long reaction time and harsh conditions (e.g., elevated temperature and special catalysts/solvents) in conventional conversions. In this study, we developed a metal-free, efficient, mild, and wide microdroplet/thin-film method to construct 18 3-substituted indoles from various substituted aromatic aldehydes and indoles with good reaction yields (42-69%) and fantastic reaction acceleration (1.32 × 103 rate acceleration factor relative to the bulk reaction). By spraying 0.8 mol L-1 reactants at 300 μL min-1, the rate of the microdroplet/thin film product was scaled up to 1.72 g h-1. Overall, the microdroplet/thin-film method offered several advantages including high efficiency, mild conditions, wide scope, and gram-scale ability, making it attractive for synthesizing 3-substituted indoles under the requirements of sustainable chemistry.

Anthocyanin stability and antioxidant capacity of Clitoria ternatea incorporated jelly

Antioxidants from natural sources from Clitoria ternatea (butterfly blue pea flower) have recently received great attention as an increase in oxidative stress disease is concerning. One of the most useful bioactive compounds from C. ternatea extract is anthocyanin, which is generally unstable due to various factors. Therefore, the extraction process needs to be optimized with a condition that favours the stability of anthocyanin. The effect of sugar as a preservative on the anthocyanin stability and antioxidant capacity of C. ternatea has been studied on anthocyanin-rich jelly formulated in two different conditions using dried butterfly blue pea flowers. The extract (E), jelly with sugar (JWS), and jelly without sugar (JWOS) were analyzed for their anthocyanin stability and antioxidant capacity for 11 weeks at two storage temperatures (25°C and 4°C). Total anthocyanin content was determined by the pH differential method, while antioxidant capacity was determined by a common 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. After two weeks of storage, the total anthocyanin content in the extract and jelly samples decreased over time, with no major difference between the samples in temperature and the presence of sugar. However, the extract alone possessed a higher and better stability of anthocyanin content (56.27 µg/ mL to 7.19 µg/mL) after 14 days of storage at both temperatures (25°C and 4°C) compared to the processed food. It might be due to the processing effect on jelly samples during the analysis that might influence the final anthocyanin content (TAC). On the other hand, jelly incorporated with anthocyanin gives a significantly higher and more stable free radical scavenging activity than the extract, with no remarkable difference between jelly with sugar and without sugar at a different temperature. In addition, anthocyanin pigments from extract and jelly samples steadily degrade during storage at 25°C after 35 days of storage, which dramatically impacts colour quality and may also affect its nutritional properties. In conclusion, their food preparation (in extract or processed form) and storage temperature highly affected anthocyanin stability and its antioxidant capacity. 1. Introduction The current lifestyle of commonly consuming processed foods, lack of exercise, and exposure to a wide range of toxins may lead to a condition defined as oxidative stress. It is marked by an imbalance in the formation and buildup of reactive oxygen species (ROS) in cells and tissues, with the biological system's inability to detoxify these reactive products that worsen the situation (Sharifi-Rad et al., 2020). From another point of view, modernization also brings a new chapter to technological and scientific innovation, which help

A comparative evaluation of physical characteristics, bioactive compounds, and antioxidant capacity of Rhodomyrtus tomentosa berry by different drying methods

The Rhodomyrtus tomentosa berry (RTB) has attracted the attention of researchers for its medicinal and nutritional characteristics. However, there is limited research on the influence of different drying methods on RTB quality. The objective of this work was to evaluate the effect of vacuum microwave drying (VMD), heat pump drying (HPD), and sun drying (SD) on the quality characteristics, microstructure, and antioxidant capacity of RTB. Results indicated that compared with HPD and SD, VMD enhanced the quality of RTB and could complete the drying process in a shorter time (3.6 hrs). In addition, VMD was able to retain a higher level of bioactive compounds and antioxidant capacity with an improved rehydration ratio. The microscopic images showed that the VMD had an obvious porous structure, facilitating the water evaporation. This suggested that VMD is an efficient drying technology for producing high-quality dried products.

The Potential of Flavonoid Antioxidants in Dragon Fruit Skin (Selenicereus undatus) for Cardiovascular Health

This study aims to examine the potential of flavonoid antioxidants found in dragon fruit peel (Selenicereus undatus) in supporting cardiovascular health. Flavonoids, as bioactive compounds with antioxidant properties, have an important role in protecting the body from oxidative stress that can damage cells and tissues, including the cardiovascular system. With a qualitative approach through literature studies and library research, this study analyzes secondary data from various scientific sources to explore the effects of flavonoids on reducing the risk of cardiovascular disease. Studies show that the flavonoid content in dragon fruit peel is able to suppress the lipid oxidation process, lower cholesterol levels, and inhibit inflammation which is a major risk factor for cardiovascular disease. In addition, the high antioxidant activity of these flavonoids has the potential to strengthen the walls of blood vessels and improve blood circulation, so that it can prevent atherosclerosis and hypertension. The results of this study support the use of dragon fruit peel as a source of natural antioxidants that have the potential to be used as a supplement in the prevention of cardiovascular diseases. A further recommendation is to conduct clinical research to test the effectiveness of flavonoids from dragon fruit peel in cardiovascular health applications in more depth.

Seaweed-Derived Bioactive Compounds: Potent Modulators in Breast Cancer Therapy.

Cancer remains a major global health concern, with breast cancer being particularly challenging. To address this, new therapeutic strategies are being explored, including natural alternatives. Seaweeds, rich in bioactive compounds, have gained attention for their therapeutic potential. Traditionally valued for their nutritional content, seaweed-derived compounds such as polysaccharides, polyphenols, sterols, vitamins, minerals, and carotenoids have shown anti-cancer properties. These compounds can modulate key cellular processes like apoptosis, angiogenesis, and inflammation-crucial in cancer progression. Their antioxidant, anti-inflammatory, and immunomodulatory effects make them promising candidates for complementary cancer therapies. Key bioactive components like fucoidans, laminarins, phlorotannins, and carotenoids exhibit anti-proliferative, pro-apoptotic, anti-angiogenic, and anti-metastatic properties. Recent studies focus on the ability of these compounds to induce apoptosis in cancer cells. This review highlights the chemical constituents of various seaweed species with anti-tumor activity, their mechanisms of action, and the potential for integration into cancer treatments. It also addresses challenges in clinical applications and outlines future research directions for leveraging these marine resources in breast cancer therapy.

Immune-Related Gene Expression Responses to In Ovo Stimulation and LPS Challenge in Two Distinct Chicken Genotypes

Background: In ovo stimulation introduces bioactive compounds, such as prebiotics, probiotics, or synbiotics into incubating eggs to enhance gut health and immune system development in chickens. This study aimed to determine the genetic and environmental effects modulating responses to in ovo stimulation in commercial broilers and Green-legged Partridge-like (GP) native chickens. Methods: Eggs were stimulated on day 12 of incubation with prebiotics (GOS—galactooligosaccharides), probiotics (Lactococcus lactis subsp. cremoris), or synbiotics (GOS + L. lactis), with controls being mock-injected. Hatched chicks were reared in group pens and challenged with lipopolysaccharide (LPS) on day 42 post-hatching. Cecal tonsils (CT) and spleens were harvested 2 h post-challenge. RT-qPCR was used to analyze the relative gene expression of cytokine genes: IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17. Results: The results show that genotype influenced the expression of all immune-related genes, with broiler chickens exhibiting stronger innate inflammatory responses than native chickens. LPS induced both mucosal (CT) and systemic (spleen) immune responses in broilers but only systemic (spleen) responses in native chickens. Conclusions: In ovo stimulation had less of an impact on cytokine gene expression than LPS challenge. Broilers expressed higher inflammatory immune responses than GP native chickens.

Data Mining for the Characterization of a Paper Prototype Obtained with Bacterial Cellulose Derived from Banana and Pineapple By-Products

The primary objective of this research is to evaluate the feasibility of two of the most prevalent agricultural residues in Ecuador, banana peels and pineapple peels, as a carbon source in the culture medium of Komagataeibacter hansenii for the production of bacterial cellulose (BC) and BC-based paper. This analysis includes an assessment of the productivity parameters of the obtained BC and the quality parameters of the BC-based paper, employing multivariate statistical methodologies. The experimental design consisted of fifteen treatments: T1 served as the control using the standard HS medium, while treatments T2–T8 used banana peel extracts (BPE), and treatments T9–T15 used pineapple peel extracts (PPE) at concentrations from 10% to 40% (v/v). Extracts were prepared with tailored pretreatments for each type of peel to optimize bioactive compound recovery. Standardized fermentation and purification conditions were applied, and once the cellulose was obtained, additives and coating agents were incorporated to produce paper samples from each treatment. The results indicated that higher BPE concentrations (T5, T6, T7, and T8) correlated significantly with increased Weight and Yield of BC, as well as improved grammage and water content in the BC-based paper. This highlights that efficient paper production is influenced by the quality of the bacterial cellulose used, with BPE-based media yielding optimal results due to their nutrient composition, which promotes bacterial growth and metabolic activity. This approach suggests a pathway for advancing sustainable and economical paper production.

Exploring the antimicrobial potential of pomegranate peel extracts (PPEs): Extraction techniques and bacterial susceptibility.

Antimicrobial resistance is increasing globally and is one of the major public health concerns. This highlights the need to search for new antimicrobial agents. Natural fruit by-products are a rich source of bioactive compounds. Pomegranate (Punica granatum) fruit is particularly rich in phenolic bioactive phytochemicals. These compounds are known for their antioxidant, anti-inflammatory, and anticancer properties. Furthermore, they exhibit a broad spectrum of antimicrobial effects. Bioactive phytochemicals are found mainly in peel (exocarp and mesocarp), which constitutes about 50% of the whole fresh fruit. This study utilized pomegranate of Jordanian origin to evaluate the antimicrobial activity of different Pomegranate peel extracts (PPEs) alone and/or in combination with antibacterial agents against four bacterial strains. Different solvents and extraction methods were employed to obtain the PPEs. A key focus was to explore the enhancement of antibacterial activity against gentamicin-resistant Pseudomonas aeruginosa (P. aeruginosa) when microwaved aqueous extracts are combined with gentamicin. The antibacterial activity of PPEs varied depending on the extraction method and the solvent used. Notably, the aqueous macerate and microwave-assisted extract showed high potency and similar activity against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and P. aeruginosa (MICs 12.5, 25, and 25 μg/μL, respectively for both aqueous extracts). In contrast, Proteus mirabilis (P. mirabilis) was more susceptible to the inhibitory activity of organic PPEs with a MIC of 25 μg/μL recorded with the use of ethanolic solvents. Bacterial antagonistic activity was observed against gentamicin-resistant P. aeruginosa, particularly when lower concentrations (3.125, 1.562, 0.781, and 0.39 μg/μL) of microwaved aqueous PPEs were evaluated in combination with different concentrations of gentamicin. In conclusion, pomegranate peels, a natural and safe by-product, demonstrate promising antimicrobial potential. Furthermore, combining PPEs with conventional antibiotics shows promise in addressing antibiotic resistance, highlighting their potential role in treating infectious diseases.

Nickel/Photoredox Catalyzed Aryl-Alkyl Cross-Coupling with Alkyl Boronic Esters as Radical Precursors.

Nickel/photoredox dual catalyzed cross-coupling of aryl halides with alkylboron compounds is one of the effective methodologies for the construction of C(sp2)-C(sp3) bonds. Although elegant results have been achieved by using alkyl trifluoroborates as alkyl radical precursors, the generation of alkyl radicals from readily available alkyl boronic esters is still limited due to their high oxidation potential. We disclosed here that activation of alkyl boronic esters by MeOLi is highly efficient for the generation of alkyl radicals under photocatalysis conditions. The reaction featured with a wide substrate scope, high functional group tolerance, and late-stage modification of bioactive substances. In addition, the method was also successfully extended to alkyl boronic acids. Experimental and computational mechanistic studies indicated that the cross-coupling likely proceeds via a Ni(I)-catalyzed pathway.

Non-Targeted Metabolomics Analysis Reveals Metabolite Profiles Change During Whey Fermentation with Kluyveromyces marxianus

Background: Whey fermentation could produce bioactive substances with immunomodulatory effects, metabolic syndrome modulation, and antioxidant properties, thereby imparting functional characteristics to products and facilitating the development of novel foods with health-promoting potential. Methods: A non-targeted metabolomics approach using liquid chromatography–mass spectrometry (LC-MS) was employed to investigate changes in the metabolite profiles of whey fermented by Kluyveromyces marxianus strain KM812 over varying fermentation durations. Results: The findings demonstrated a progressive enrichment of metabolites over time. A total of 151 differential metabolites were identified and categorized primarily into amino acids, peptides, and analogues, fatty acids and conjugates, and carbohydrates and conjugates, as well as benzoic acids and derivatives. The highest relative content of whey metabolites was observed at 48 h of fermentation, with a cumulative increase of 1.45-fold, 1.49-fold, 3.39-fold, and 1.24-fold for peptides and amino acids, peptides, and analogues, fatty acids and conjugates, and carbohydrates and conjugates, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed associations with 23 specific metabolites and delineated 9 metabolic pathways, predominantly involved in amino acid and lipid metabolism. Conclusions: Based on the above, KM812 could effectively degrade macromolecular substances in whey into small molecules such as L-isoleucine, ornithine, betaine, α-linolenic acid, and palmitoleic acid, thereby influencing the nutritional and functional properties of whey. In-depth analysis of the metabolic products in KM812-fermented whey could provide a theoretical basis for the development of functional foods derived from small molecules in the future.

Neurodegenerative Marine Algae Bioactive Compounds: A Viable Cure to Treat Amyotrophic Lateral Sclerosis (ALS): A Review

Introduction: Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease that causes muscle weakness, paralysis, and death, develops when motor neurons begin to die. There are few proven treatments for ALS, and because the disease is incurable, the exact cause is unknown, making it a devastating condition. According to recent research, marine algae may contain bioactive substances that can be used to treat ALS. Methods: The comprehensive review of recent publications focused on bioactive compounds extracted from various species of marine algae, including their mechanisms of action against oxidative stress, neuroinflammation, and apoptosis in ALS. The publications were reviewed in scientific journals (ScienceDirect, Springer, Taylor & Francis, and MDPI) and indexed in several databases (Scopus, Web of Science, PubMed, Google Scholar, and so on). Discussion: Compounds derived from marine algae, including polyunsaturated fatty acids, fucoxanthin, and polysaccharides, exhibit potential neuroprotective effects by modulating neuroinflammation and oxidative stress levels. Fucoxanthin, fucosterol, and alginate demonstrated potential in mitigating oxidative damage and inflammation, which are critical factors in the pathogenesis of ALS. Conclusion: Bioactive compounds obtained from marine algae demonstrate considerable potential as therapeutic agents for ALS, owing to their capacity to influence multiple pathways linked to oxidative stress and neuroinflammation. Further investigation is required to comprehend their mechanisms and medicinal value, as well as develop novel alternative treatments for ALS.

Sustainable Lipase Immobilization: Chokeberry and Apple Waste as Carriers

In the modern world, the principles of the bioeconomy are becoming increasingly important. Recycling and reusability play a crucial role in sustainable development. Green chemistry is based on enzymes, but immobilized biocatalysts are still often designed with synthetic polymers. Insoluble carriers for immobilized biocatalysts, particularly those derived from agro-industrial waste such as mesoporous lignocellulosic materials, offer a promising alternative. By using waste materials as support for enzymes, we can reduce the environmental impact of waste disposal and contribute to the development of efficient bioprocessing technologies. The current study aimed to assess the possibility of using apple and chokeberry pomace as carriers for the immobilization of Palatase 20000L (lipase from Rhizomucor miehei). The analysis of lignocellulosic materials revealed that chokeberry pomace has a higher neutral detergent fiber (NDF) and lignin contents than apple pomace. Moreover, Scanning Electron Microscopy (SEM) observations indicated similar compact structures in both pomaces. The lipase activity assays demonstrated that immobilization of lipase from R. miehei onto apple and chokeberry pomace improves their properties, especially the synthetic activity. The findings highlight the potential of utilizing fruit pomaces not only as a source of bioactive compounds but also in enhancing enzyme stability for industrial applications.