Phytochemical Analysis Of Methanolic Extract Research Articles

Attenuation of paracetamol-induced hepatotoxicity in Ajuga bracteosa extract treated mice

Ajuga bracteosa (Ab) has tremendous medicinal value with long-established disease curing potential. The present study aimed to assess the hepatoprotective potential of Ab extracts in paracetamol-induced hepatotoxicity in mice. Group I (normal control) were treated with saline 1 ml/kg BW orally for 7 days while Group II (toxicant control) received saline 1 ml/kg BW for 6 days and Paracetamol (1000 mg/kg BW) on day7of the treatment. Group III received Standard drug silymarin (100 mg/kg BW) for 6 days and Paracetamol (1000 mg/kg BW) on day 7of treatment. Groups IV andV were administered with methanol extract (ME) 200 mg/kg BW and aqueous extract (AE) 1000 mg/kg BW for 6 days and Paracetamol (1000 mg/kg BW) on day 7th of the study. Both extracts showed hepatoprotective potential against the toxic effects of paracetamol, evidenced by serum analysis of biomarkers involved in liver injury and histopathological findings. Hepatotoxic mice pretreated with Ab plant extract or silymarin exhibited significant decrease in ALP, AST, and ALT enzyme level while GSH levels were markedly increased. According to histological observations, groups treated with PCM (toxicant control) showed significant necrosis and lymphocyte infiltration, while groups treated with silymarin and Ajuga bracteosa plant extract showed preservation of the normal liver structural features. The phytochemical analysis of ME and AE of Ab showed the presence of glycosides, phenolic compounds, tannins, fats, saponins, flavonoids, terpenes, oils, and fats. The antioxidant activity of these two extracts was determined by nitric oxide assay, DPPH assay, and ferric reducing power assay. The methanolic extract exhibited the highest antioxidant potential (78.09 ± 0.0806). The antioxidant potential of aqueous extract was 73.08 ± 0.248. The reducing power for methanolic extract and ascorbic acid (standard) 500 μg/ml was 0.933 and 0.987 respectively. The anti-inflammatory activity of both extracts was demonstrated by in vitro methods, namely albumin denaturation, proteinase inhibition, and membrane stabilization assays. The study suggests that Ab extracts have competence for attenuating inflammation, oxidants, and hepatotoxicity.

Bay Laurel of Northern Morocco: A Comprehensive Analysis of Its Phytochemical Profile, Mineralogical Composition, and Antioxidant Potential

Laurus nobilis, sometimes referred to as laurel, has been used for medicinal and culinary purposes for a very long time. The main subjects of this study are the phytochemical composition, mineralogical profile, and potential antioxidant properties of Laurus nobilis in Tangier, Northern Morocco. For phytochemical analysis of methanolic extracts, high-performance liquid chromatography (HPLC-UV-MS) was used, and Fourier transformation infrared spectroscopy (FT-IR) was used to identify each individual component. Minerals were studied by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and wavelength dispersive X-ray fluorescence (WD-XRF). Total tannin, flavonoid, and phenolic amounts were quantified using aqueous and methanolic extracts. The antioxidant properties were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays. Research has revealed a complex array of phytochemicals, including tannins, flavonoids, and phenolic acids. Mineral analysis has revealed the existence of vital components that are beneficial to health. Comparing the methanolic extract to the water extract, it demonstrated higher levels of phenols, flavonoids, and tannins as well as stronger antioxidant activity, indicating greater health benefits. This comprehensive study highlights the importance of Laurus nobilis from Northern Morocco as a reliable botanic resource with potential pharmaceutical, nutritional, and cosmetic uses.

Assessment of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, total antioxidant activity, ferric reducing power and phytochemical analysis of methanolic extract of Malva parviflora

Assessment of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, total antioxidant activity, ferric reducing power and phytochemical analysis of methanolic extract of Malva parviflora

Evaluation of antimicrobial activity and bioactive compound analysis of Verbascum thapsus L. A folklore medicinal plant

BackgroundThe prevalence of microbial infectious diseases is a major contributor to morbidity and mortality on a global scale due to the emergence of antibiotic-resistant microbes, despite the fact that many new antibiotics have been introduced for several decades. In present scenario, the potential utilization of natural plant derived extracts for medicinal and therapeutic purposes has increased remarkably due to adverse effects of synthetic drugs and resistance among pathogenic microbes. Aim of the studyVerbascum thapsus L. (Common mullein) has been traditionally used as a medicine for lung, skin and throat and many other disorders since time immemorial, therefore, the current study was aimed to evaluate the antimicrobial activity of leaf and root extracts of Verbascum thapsus against pathogenic bacterial and fungal strains and its phytochemical composition by spectral studies. MethodsSoxhlet extraction method was employed for extraction process by using varying polarity solvents like hexane, ethyl acetate and methanol. Phytochemical analysis of methanol extract was determined by Fourier Transform-Infrared Spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC–MS). Antimicrobial activity was evaluated by determining the MIC and MBC/MFC of each extract by broth dilution method. ResultsThe antimicrobial competence of each extract sample was evaluated against six pathogenic bacterial strains and three fungal strains. Methanolic extract of leaves and the ethyl acetate extract of roots of Verbascum thapsus exhibited significant zones of inhibition against all the tested microorganisms. The highest inhibition zone was observed against the bacterial strain Bacillus subtilis (24.1±0.18 mm) followed by Streptococcus pyogenes (22.9±0.64 mm) and the fungal strain Aspergillus niger (22.8±0.55 mm) at a concentration of 500 μg/ml. Methanolic leaf extract strongly inhibited the growth of tested microbial strains with MIC ranged from 15.625 to 125 μg/ml. The FTIR and GCMS analysis confirms the presence of bioactive compound, possessing broad range of therapeutic activities. ConclusionThis Presence of various important phytoconstituents and broad-spectrum antimicrobial activity points Verbascum thapsus as a promising source for the isolations of molecules responsible for antimicrobial activities.

Phytochemical Analysis of Methanolic Extracts of Leaves of Some Selected Medicinal Plants of Jorhat District, Assam, India

Phytochemical Analysis of Methanolic Extracts of Leaves of Some Selected Medicinal Plants of Jorhat District, Assam, India

Evaluation of Anti-inflammatory activity of Jacquemontia caerulea Leaf Methanolic Extract in Wistar rats

Objective: To evaluate anti-inflammatory activity of methanolic extract of obtained from the leaves of plant, Jacquemontia caerulea in Wistar rats. Methods: Fresh leaves of Jacquemontia caerulea (Family: Convolvulaceae) were extracted using methanol and subjected to phytochemical analysis using standard procedures. The anti-inflammatory nature of the methanolic leaf extract (JCLME) was assessed on Wistar rats, with Indomethacin serving as the standard reference drug. Two established methods were employed: Paw edema method by using Carrageenan and Cotton pellet granuloma method. Results: Phytochemical analysis of methanolic extract was found to have phytoconstituents such as saponins, flavonoids, glycosides, steroids, proteins, amino acids, phenols, and tannins. The anti-inflammatory evaluation showed that JCLME produced anti-inflammatory effect. In the paw edema model by Carrageenan, JCLME demonstrated remarkable (p<0.05) inhibition of paw edema with reduction of 23% and 30% at dose of 250 mg/kg and 500 mg/kg, respectively. Similar result was observed in the Cotton pellet granuloma method. Conclusion: The leaf methanolic extract of Jacquemontia caerulea contains a high concentration of various phytochemicals and exhibits significant (p<0.05) anti-inflammatory effect in both Carrageenan-induced paw edema and Cotton pellet granuloma model in Wistar rats.

Phytochemical Characterization and Assessment of Crude Extracts from Justicia adhatoda for Phytotoxic and Cytotoxic Activity

Background. The aim of the study was to investigate the cytotoxicity, phytotoxicity, and proximate and phytochemical analysis of methanolic extracts of the leaves of Justicia adhatoda. Methods. Methanolic leaf extract of J. adhatoda was screened for phytotoxic activity by using root length inhibition and seed germination assays. Cytotoxic activity was calculated using brine shrimp lethality bioassay. Plant extracts were also investigated for their proximate composition. The presence of several phytochemicals was tested by employing different methods. Results. Decrease in seed germination and root length, 62.67% and 83.11%, was proportional to the increasing concentration of the methanolic extract of the plant. Cytotoxicity assay results indicated that the methanolic extract possessed significant cytotoxic potential with an LC‐50 of 217 µg/ml. Proximate analysis revealed that the leaves of J. adhatoda contain 9.4% moisture, 90.6% dry matter, 19.25% crude protein, 4.5% crude fat, 8.0% crude fiber, and 11.5% total ash. Conclusion. Methanolic extracts of J. adhatoda leaves showed significant cytotoxic effects and may have potential use in medicine. The J. adhatoda foliar extract shows good inhibitory effects against seed germination and root growth. Therefore, it might be used as soil additive in crops to control weeds. Further research is required to detect and isolate phytotoxins from the plant that might replace synthetic herbicides with eco‐friendly herbicides.

Phytochemical Analysis and Biological Activities of Curcumin

Curcumin is a bright yellow phytochemical derived from the rhizome of Curcuma longa. Curcumin possesses a wide range of biological effects such as antimicrobial, anti-proliferative, antioxidant, anti-inflammatory, anti-diabetic and neuroprotective activities. Such versatility makes curcumin a promising lead compound for the development of new derivatives that may have a role in the management of numerous illnesses like cancer, diabetes and Alzheimer’s disease. These biological effects of turmeric have been attributed to its constituent curcumin that has been widely studied for its anti-diabetic, anti-obesity, anti-oxidant, wound healing and anti-cancer effects and phytochemical analysis of methanolic extract was done. As a result of extensive epidemiological, clinical, and animal studies several molecular mechanisms are emerging that elucidate multiple biological effects of curcumin. The purpose of the study was concentrated on in vitro and in vivo studies on the biological effects of Curcumin, preliminary phytochemical screening of the methanolic extract of the turmeric rhizome and determined the antioxidant activity of curcumin by employing in vitro antioxidant assays such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH)scavenging. The solvent extracts tested positive for the presence of bioactive constituents alkaloids, flavonoids, phenols, saponins, Terpenoids, cardiac glycosides and fixed oils and fatty acid. DPPH demonstrated total antioxidant activity.

Phytochemical Profile, Antioxidant and Antiproliferative Activity from Leaves and Seeds of Bixa orellana L. from the Yucatán Peninsula, Mexico

Background In Mexico, there is an ancestral custom about the knowledge of the medicinal use of plants. Bixa orellana L. is a plant species that represents one of the main crops used in traditional medicine in southeastern Mexico. Background studies on its biological properties have been conducted on accessions harvested in Conkal, Yucatan, Mexico. Objectives In this work, the idea was to perform a phytochemical analysis of methanolic extracts of B. orellana. Materials and Methods Phytochemical screening was performed on methanolic extracts of leaves and seeds from two accessions of B. orellana, as well as on their antioxidant and antiproliferative activities. Results The phytochemical screening of the methanolic extracts of the leaves revealed the presence of tannins, terpenes, and saponins. A higher total content of phenols and flavonoids in leaves was also found compared to seeds, which coincides with a higher antioxidant activity in leaves compared to seeds. Regarding antiproliferative activity, the seeds and leaves of accession 3 showed the best results. Conclusion Accession 3 has better bioactive properties and can be a candidate in the search for antiproliferative and antioxidant compounds; this is the first study that associates this plant with this activity and demonstrates the chemical differences that exist between the accessions of B. orellana.

Bioactive principles, antibacterial and anticancer properties of Artemisia arborescens L.

Artemisia arborescens is a medicinal and aromatic plant used in traditionally by the people of Saudi Arabia. This research attempts to evaluate the bioactive constituents of the plant using organic solvents, as well as the antibacterial and anticancer properties of plant extracts. The Phytochemical analysis of methanol extract revealed eleven bioactive constituents, identified by comparing their retention periods and GC-MS profiles to account for 52.45 percent of the studied extract. In the meantime, the extract of pet ether had demonstrated the presence of sixteen significant constituents, six of which were distinct sesquiterpene derivatives. In lipophilic plant extract, three higher alkanes made up 12.49% of the total. These higher alkanes were tetratriacontane (6.55%), hentriacontane (4.17%), and octacosane (1.77%). Studies on antimicrobial activity have revealed that both methanolic and petroleum ether extracts had a broad spectrum of activity against specific human pathogens. Both extracts, however, failed to exhibit any anti-Candida albicans activity. Methanolic extract not shown inhibition in the cell growth of MCF-7 cell, but petroleum ether extract had shown significant anti-cancer activity against MCF-7 cell with an IC50 of 13.49 µg/mL. the results obtained show that A. arborescens have a lot of potential for further research into variety of biological functions, against cancer and microbes.

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

Plants remain a vibrant source of diverse bioactive phytochemicals that are secondary metabolites protecting them from infections and predations. Magnolia champaca is reported to possess a multitude of phytochemicals. In the present study, the phytochemical constituents of the methanolic extract of Magnolia champaca leaves were analysed qualitatively and with gas chromatography-tandem mass spectrometry (GC-MS/MS). Fourier transform infrared (FTIR) spectroscopic analysis was performed to identify the chemical nature of the extract and to find structurally similar compounds. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, terpenes, glycosides, phenols, steroids, tannins and saponins. FTIR spectroscopic analysis revealed structurally related compounds. GC-MS/MS analysis revealed the presence of 99 diverse compounds with varied biological activities, among which 1,2,4-butanetriol, n-hexadecanoic acid, cis vaccenic acid, phytol, trans longipinocarveol and caryophyllene oxide were found predominantly. Thus, the identification and characterisation of the phytochemicals in the extract favour the development of novel therapeutic agents.

Phytochemical analysis of methanolic extracts of Elymus repens, Typha angustifolia and Caralluma edulis

Introduction: Medicinal plants have been the source of medicine to all civilizations for hundreds of years. In classical medicine, Elymus repens (Poaceae) is consumed as a diuretic, emollient and tonic. It also soothes the pain and spasm in the urinary tract as well as treats the condition of urolithiasis (formation of kidney stones) and urinary tract infections (UTI). In Chinese folk medicine, T. angustifolia is employed to improve the microcirculation, improve body’s defense mechanism, activate contractions of uterus, heal atherosclerosis, treat wounds and promote the differentiation and trigger the division of keratinocytes in humans. The traditional healers recommend C. edulis to cure hypertension, rheumatism, leprosy, diabetes, infections, gastric issues and Alzheimer disease. Objectives: This study was aimed to investigate different phytochemical constituents in methanolic extracts of E. repens (plant body and roots), T. angustifolia (stem and fruiting body) and C. edulis. Methodology: Phytochemical potentials of methanolic extracts of plants were investigated using standard procedures. Results: Phytochemical analysis of E. repens crude methanol extract confirmed the existence of tannins, saponins, flavonoids and anthraquinones. However, phenols were absent in methanol extract of roots. T. angustifolia stem contained anthraquinones. Tannins, saponins, phenols and flavonoids were absent from T. angustifolia stem. C. edulis contains saponins, tannins, flavonoids and anthraquinones except phenols. Conclusion: Results of this study shows that E. repens, T. angustifolia and C. edulis are enriched with different phytochemical constituents hence validating their uses for the treatment of various diseases.

On Review - Brief Cryptostegia Grandiflora Linn Roxb

Medicinal plants have been in use since 5000 years. Oldest known herbal is Pen- t’sao written by emperor Shen-Nung around 3000 years. It contains 365 drugs, one for each day of the year. Indians worked meticulously to examine and classify the herbs. Charaka made 50 groups of 10 herbs, each of which would suffice an ordinary physician's need. Cryptostegia grandiflora Linn Roxb. (Apocynaceae), commonly known as “Vilayti vakundi” is found all in dry area in India. The study includes preparation of different extracts by successive solvent extraction for detail analysis. Fluorescence analysis of different successive extract and powder were noted under UV light and normal ordinary light, which signifies there characteristics. Preliminary qualitative chemical test for different extracts showed presence of glycosides, flavonoids, fixed oil and fats, phenolic compounds, protein and amino acids, tannins, gum and mucilage and carbohydrates. Qualitative phytochemical analysis of methanol extracts of leaves of Cryptostegia grandiflora Linn Roxb showed the presence of cardiac and saponins glycosides, tannins, flavonoids, proteins.

Phytochemical analysis and biological activities of solvent extracts of Ocimum basilicum leaf and Curcuma longa rhizome and its effect on Disonycha xanthomelas

The study was conducted in Coimbatore, Tamil Nadu. In this study an attempt was made to control Disonycha xanthomelas using Ocimum basilicum (leaf) and Curcuma longa (rhizome) solvent extracts. From the phytochemical analysis of methanol extracts of Ocimum basilicum leaf and Curcuma longa rhizome determined the presence of Alkaloids, Flavonoids, Tannins, Saponins, Glycosides, Steroids, Terpenoids, Phenols, Quinone, and Phlobotannins. In antifeedant analysis the mortality rate of Disonycha xanthomelas is increased on the basis of Petroleum ether and methanol extract concentration. After 24 to 72 hours treatment the mortality rate percentage of Disonycha xanthomelas were 3.00% to 6.66% in petroleum ether extract of Ocimum basilicum leaf and 3.60% to 6.00% were observed in Curcuma longa rhizome extract. In methanol extracts of Ocimum basilicum leaf and Curcuma longa rhizome showed 2.33% to 6.00% and 2.30% to 6.00%. Ocimum basilicum leaf extracts in both solvents are slightly more effective than petroleum ether and methanol extracts of Curcuma longa against Disonycha xanthomelas.

In Vitro Antioxidant and Antimicrobial Activity of Isolated Flavonoid from the Methanolic Extract of Spermacoce hispida Leaves

The purpose of this research was to separate the flavonoid component of the Spermacoce hispida plant that is responsible for its medicinal effects. A methanolic extract of Spermacoce hispida leaves was used to study the separation of flavonoids from a plant component. Phytochemical analysis of methanol extract confirmed the presence of flavonoid compounds. The extracted flavonoid component was refined using thin-layer chromatography and column chromatography. The structures of the isolated compounds were characterised using UV-Visible, FT-IR, 13C, 1H NMR, and Mass spectroscopy. Therefore, 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[-Lrhamnopyranosyl-(16)—D-glucopyranosyloxy] was given as the name for the isolated molecule. There has to be further biological investigation of this plant's isolated flavonoid. Flavonoids are a distinct class of plant secondary metabolites having pharmacological benefits that have been used for centuries in traditional medicine. They support a diverse array of medical procedures that may provide novel and therapeutic medication discoveries. Flavonoids' potential as antioxidants in the face of oxidative stress has been the subject of a lot of recent research. To what extent flavonoids may serve as antioxidants and as antimicrobials is explored in this study.

Qualitative and Quantitative Phytochemical Analysis of Methanol Extracts of Phragmanthera incana (schum) Leaves Parasitized on South-West-Nigeria Host Trees

Phragmanthera incana (Schum), a specie of mistletoe, belonging to the family Loranthaceae, is a hemi-parasitic plant growing on trees in South-Western part of Nigeria. The phytochemical constituents of P. incana from four host trees; Psidium guajava (guava), Cola acuminata (kolanut), Anacardium occidentale (cashew) and Mangifera indica (mango) were analysed qualitatively and quantitatively following standard protocols. The Qualitative screening showed the presence of tannins, anthraquinone, flavonoids, phenols, reducing sugar, cardiac glycosides, terpernoids, saponin and steroids in all the four selected host trees. Quantitative evaluation showed a significantly high (p < 0.05) Phenol and Steroid contents in P. incana from cashew tree when compared with P. incana from guava, kolanut and mango trees. A significant increase (p < 0.05) in Saponin, Terpernoids and Cardiac glycosides was recorded in P. incana from mango tree when compared with P. incana from cashew, guava and kolanut trees while a significantly high (p < 0.05) reducing sugar was in P. incana from kolanut tree when compared with P. incana leaves from cashew, guava and mango trees. It can be concluded that the plant has immense potential to be used in the area of pharmacology and as a prospective target for drug formulation. Key Words: Phytochemicals, Phragmanthera incana, Mistletoes, Hemi-parasitic plants

Antioxidant-rich fraction of Amomum subulatum fruits mitigates experimental methotrexate-induced oxidative stress by regulating TNF-α, IL-1β, and IL-6 proinflammatory cytokines.

The culinary spice Amomum subulatum was assessed for its phytochemical composition, in vitro antioxidant potential, and in vivo ameliorating effect against methotrexate (MTX)-induced toxicities. Phytochemical analysis of methanolic extract of A.subulatum dry fruits (MEAS) confirmed the presence of different bioactive secondary metabolites. MEAS scavenged reactive free radicals and inhibited lipid peroxidation in vitro. To confirm the antioxidant efficiency of MEAS, in vivo experiment was carried out in which MTX was administered to induce oxidative stress. Co-administration of MEAS reduced MTX-induced hepatic, renal, and pulmonary toxicities via significantly (p<.01) enhancing antioxidant status and reducing oxidative stress. MTX treatment significantly (p<.01) increased liver and kidney toxicity markers and increased proinflammatory cytokine (TNF-α, IL-1β, and IL-6) levels. However, co-administration of MEAS significantly (p<.01) reduced their levels, and tissue histopathology confirmed the protective effect of MEAS in maintaining normal tissue architecture following MTX treatment. Protective effect of MEAS is accredited to the antioxidant and anti-inflammatory properties exhibited by bioactive compounds in MEAS. PRACTICAL APPLICATIONS: Amomum subulatum (Black cardamom) is a folkloric and culinary spice used for its organoleptic, nutritional, and medicinal properties. This study demonstrated the phytochemical composition and antioxidant potential of methanolic extract of A.subulatum dry fruits (MEAS). Toxicities associated with MTX therapy limit its clinical application. MEAS attenuated methotrexate-induced oxidative stress, inflammation, and associated organ damages, suggesting the possible therapeutic application of A.subulatum in reducing oxidative stress and associated diseases. Our results showed that A.subulatum is a potential functional food, which may be used for the betterment of health due to its richness in antioxidant and anti-inflammatory phytochemicals.

Phytochemical Screening and Mass Spectrometric Analysis to Unveil the Pharmacological Properties of Weed Euryops pectinatus

Euryops pectinatus ‘Viridae’ weed belonging to the family Asteraceae is commonly known as Golden Euryops or African daisy. Present investigation includes phytochemical analysis of methanol extract of Euryops pectinatus weed for the presence of various secondary metabolites. Presence of various secondary metabolites was observed including phenols, flavonoids, alkaloids, steroids, saponins, glycosides, etc. These secondary metabolites are well known for their biological activities like antioxidation, anti-cancerous, anti-microbial, cardiovascular, and anti-aging. After determination of rich secondary metabolites in the methanol extract, mass spectrometric analysis was carried out for the identification of compounds present in Euryops pectinatus. Eight compounds were identified in the methanol extract reported for nematicide, antioxidant, analgesic, antitumor, anticancerous and several other important activities. Thus phytochemical and mass spectrometric analysis would open new doors to natural product research that would be cost effective and safe for the mankind. Keywords: Euryops Pectinatus; Phytoconstituents; Bioactives; Mass-Spectroscopy; Anticancer

Proximate and phytochemical analysis of methanolic extract of Cissus quadrangularis

The present work was conducted to evaluate the proximate contents, phytochemical constituents and to screen the methanolic extract of the stem of Cissus quadrangularis for the presence of active compounds. Methanol was the solvent used for the soxhlet extraction. The dry matter content and the total ash, crude protein, crude fat, crude fibre, acid insoluble ash, carbohydrate content were determined. The chemical composition of methanolic extract of Cissus quadrangularis revealed the presence of alkaloids,, saponins, phytosterols, phenolic compounds, flavanoids, terpenoids, protein, amino acids, fixed oils, fats, tannins and cardiac glycosides. This work could be further useful to the structural elucidation and further quantification of bioactive compounds.

Phytochemical Screening, Antioxidant Profile and Cytotoxic Activity of Methanol Extract of Bergenia ligulate

Bergenia ligulata is the prominent ayurvedic herb for anticancer activity. The leaf of Bergenia ligulata was extracted with methanol and evaluated by phytochemical analysis for the content of innumerable metabolites like primary and secondary. The antioxidant efficacy was assessed through method DPPH free radical scavenging activity. Estimation of total phenolic content, total flavonoid content and tannin content were done to confirm the presence of these photochemical. The cytotoxic effect was determined against the Adrenal gland cancer cell lines (PC12 using MTT assay). It was revealed from the phytochemical analysis of methanol extract of Bergenia ligulata that tannin, saponin, cardio glycosides, protein, quinines, phenols and flavonoid were present. A significant antioxidant activity was revealed by the methanol extract 42.8+3.40%. Quantity of total phenol content, total flavonoid content and tannin content were the highest in the139.8 +9.06mg of GAE/g, 77+6.40 mg of QE/g, 70.4+6.40mg of TAE/g at 500 µg concentration. At lowest concentration of 3.12 µg/ml of methanol extract of Bergenia ligulata cell death was observed with the highest value of 61.1+4.86% respectively. The IC 50 value of methanol extract of Bergenia ligulata was found to be 53.925% µg/ml respectively against PC12 cell line. The study suggests that methanol extract of Bergenia ligulata has significantly antioxidant property. Bergenia ligulata is a good candidate for isolation of antioxidant, total phenol content, total flavonoid content, tannin and anticancer activity that can be a breakthrough for pharmaceutical industry. The antioxidant activity present in Bergenia ligulata have strong cytotoxic activity suggests that it can be considered for anti-cancer treatment.