In Vitro Antioxidant and Antimicrobial Activity of Isolated Flavonoid from the Methanolic Extract of Spermacoce hispida Leaves

Abstract

The purpose of this research was to separate the flavonoid component of the Spermacoce hispida plant that is responsible for its medicinal effects. A methanolic extract of Spermacoce hispida leaves was used to study the separation of flavonoids from a plant component. Phytochemical analysis of methanol extract confirmed the presence of flavonoid compounds. The extracted flavonoid component was refined using thin-layer chromatography and column chromatography. The structures of the isolated compounds were characterised using UV-Visible, FT-IR, 13C, 1H NMR, and Mass spectroscopy. Therefore, 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[-Lrhamnopyranosyl-(16)—D-glucopyranosyloxy] was given as the name for the isolated molecule. There has to be further biological investigation of this plant's isolated flavonoid. Flavonoids are a distinct class of plant secondary metabolites having pharmacological benefits that have been used for centuries in traditional medicine. They support a diverse array of medical procedures that may provide novel and therapeutic medication discoveries. Flavonoids' potential as antioxidants in the face of oxidative stress has been the subject of a lot of recent research. To what extent flavonoids may serve as antioxidants and as antimicrobials is explored in this study.