Phytochemical Analysis Of Ethanolic Extract Research Articles

Nutritional Innovation Using Green Seaweed (Ulva sp.) and Garlic Powder Extracts for White-Leg Shrimp (Litopenaeus vannamei) Challenged by Vibrio harveyi.

This study aimed to determine the antimicrobial effects of ethanolic extracts of Ulva sp. and garlic (Allium sativum) powder ethanolic extracts against Vibrio harveyi in vitro. The stimulatory effects of Ulva sp. extract (UE) and garlic powder extract (GPE) on the growth performance and innate immune responses of white-leg shrimp, Litopenaeus vannamei, and their challenge against V. harveyi infection were also investigated. A commercial shrimp diet (36.1% protein) was enriched with 0.5, 1.0 and 2.0gUE/kg diet and 2, 4 and 6gGPE/kg diet, whereas the control group was free of any supplement. Health juveniles of L. vannamei (average weight 2-3g) were distributed in 21 fiberglass reinforced plastic (FRP) tanks (500-L capacity) at a stocking density of 300 animals/tank to represent each treatment in triplicate. The animals were fed ad libitum on the experimental diets up to satiety four times daily for 60 days. The phytochemical analysis of ethanolic extracts of Ulva sp. and garlic powder evoked their richness of several bioactive compounds showing significant antibacterial activity against V. harveyi. The GPE exhibited a higher inhibition zone than that of the UE. The supplemented diets did not significantly affect weight gain %, final weight, feed conversion ratio, specific growth rate and survival rates of white shrimp compared to those fed on the control diet. Significant increases were observed in total haemocyte count, phagocytosis and phagocytic index of all treatments compared with the control group. There were significant increases in serum total protein, acid phosphatase activity, alkaline phosphatase, lysosomal enzyme activity, phenoloxidase activity and superoxide dismutase activity with offered diets with increasing the levels of ethanolic extracts of Ulva sp. and garlic powder up to 2.0gUE/kg diet and 6gGPE/kg diet, respectively. The ethanolic extraction of Ulva sp. and garlic powder-supplemented diet groups, particularly at treatments of 2.0 and 6gGPE/kg diet, respectively, significantly reduced the shrimp mortality induced by V. harveyi infection when compared with the control group. The net results evoked that ethanolic extraction of Ulva sp. (2.0gUE/kg) and garlic powder (6gGPE/kg diet) enhanced the immune response and disease resistance of the white-leg shrimp, L. vannamei. It is also noted that the GPE is more efficient than the UE in vitro and in vivo investigations.

Analisis Fitokimia Daun Gaharu (Aquilaria microcarpa) Sebagai Produk Teh Celup Asal Keerom, Papua

This study explores the phytochemical composition and antioxidant potential of Agarwood (Aquilaria microcarpa) leaves from Keerom, utilized in tea bags. Phytochemical analysis of ethanol extracts revealed flavonoids, alkaloids, steroids/triterpenoids, tannin gallate, and saponins. Total phenol content measured at 1.31 g GAE/100 grams, with total flavonoids at 210 mg QE/100 grams. The extract demonstrated robust antioxidant activity with an AAI value of 1.23, surpassing ascorbic acid (AAI value of 13.37). Mangiferin and quercetin were identified among the compounds in the leaves, highlighting their potential health benefits.

Characterization and Extraction of Bioactive Compounds in Ethanolic Extract of Moringa concanensis, Nimmo Leaf, Using Microwave-assisted and Soxhlet Techniques

Background: The extraction of Moringa concanensis (Nimmo leaf) was done in ethanol using Soxhlet and microwave-assisted extraction (MAE) techniques to obtain bioactive compounds. Methods: The standard techniques were used to carry out phytochemical analysis of ethanolic extract. The analysis confirmed the presence of flavonoids, phenols, steroids, alkaloids, terpenoids, glycosides, tannins, and saponins, and also the absence of anthraquinones. Moreover, the extract was evaluated for antioxidant activities using DPPH and ABTS assays. The IC50 value of the extract was also determined. Results: A clear correlation between the total phenolic content, total flavonoid content, and antioxidant activity of the leaf extracts was obtained. Conclusion: This study suggests that a high amount of phenolic compounds could be obtained from Moringa concanensis (Nimmo leaf) by using the microwave-assisted extraction technique, which may be responsible for its antioxidant activity.

Qualitative Phytochemical Analysis of Ethanolic Extracts of Hibiscus laevis Flowers and Euryale ferox Seeds

Qualitative Phytochemical Analysis of Ethanolic Extracts of Hibiscus laevis Flowers and Euryale ferox Seeds

Quantitative Phytochemical Analysis of Ethanol Extract Kelulut Bee Propolis (Trigona laeviceps)

Kelulut bees are widely bred in Kalimantan. These bees do not have a stinger and easily adapt to new environments. Another characteristic of the kelulut bee is that it produces more propolis than honey. Kelulut Bee propolis contains tannins, alkaloids, flavonoids, and phenolics bioactive compounds with the potential to heal drugs. Following the concept of 'Back to Nature', namely increasing public interest in using natural ingredients to be used as medicine. Therefore, a test is needed to strengthen the utilization of this potential and quantitative phytochemical analysis is needed to determine the levels of each compound in kelulut bee propolis. The purpose of this research was to analyze the results of the quantitative phytochemical of ethanol extract of kelulut bee propolis (Trigona laeviceps). The research used non- experimental research with quantitative laboratory examination to analyze sample content. The results showed he quantitative phytochemical analysis of ethanol extract of kelulut bee propolis (Trigona laeviceps) showed a content of tannin compound 1.85 mg/ml, alkaloid 154.31 mg/ml, flavonoid 56.83 mg/ml and phenolic 120.37 mg/ml. Ethanol extract of bee propolis (Trigona laeviceps) showed a tannin compound content of 1.85 mg/ml, alkaloids 154.31 mg/ml, flavonoids 56.83 mg/ml and phenolic 120.37 mg/ml. Alkaloid had the highest concentration of 154.31 mg/ml, while tannin had the lowest concentration of 1.85 mg/ml.

DIURETIC AND LAXATIVE ACTIVITIES OF MORINGA OLEIFERA SEEDS AND PODS IN MICE

Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thousands of years having diverse nutritional bioactive constituents. Phytochemical analysis of ethanolic extract of this plant indicated the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins (absent in seeds), alkaloids, flavonoids, saponins and steroids. In diuretic activity test, the extract showed a significant (p<0.001) effect at the dose of 200 & 400 mg/kg body weight in production of total urine volume in comparison with standard drug furosemide (5 mg/kg). Upon electrolyte analysis of excreted urine, the extract was found to increase Na+, K+, Clexcretion and may act as loop diuretics. The pods and seeds extract showed significant (p<0.01) laxative effects in mice in terms of stool production with soft consistency at the dose of 200 & 400 mg/kg in comparison with standard drug bisacodyl (10 mg/kg). The most edible part of this plant is pods which showed better diuretic and laxative effects than seeds. So, the pods may be a good source of bioactive constituents which may be used as parent compound(s) for discovering better diuretic or laxative drugs.

Phytochemical Analysis of Ethanolic Extracts of Three Sudanese Tribulus species

Phytochemical Analysis of Ethanolic Extracts of Three Sudanese Tribulus species

Qualitative and Quantitative Study of Phyto-Constituents and Antioxidant Potential of Rhizomes of Kaempferia galanga, Kaempferia parviflora and Kaempferia pulchra

Medicinal plants are the potent source of biologically active compounds and have always been of field of interest for the effective chemotherapeutic agents and offering a broad spectrum of activity with greater emphasis on preventive action. The objective of this study was to screen the phytochemicals, estimate the content of phenolic, flavonoids and alkaloids compounds and determines the antioxidant capacity of the rhizomes of Kaempferia galanga Linn, Kaempferia parviflora and Kaempferia pulchra (K. galanga, K. parviflora and K, pulchra, Zingiberaceae). Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol, flavonoids and alkaloids were determined by the well-known test protocol available in the literature. The ethanolic extract of rhizomes of K. galanga, K. parviflora and K, pulchra was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay and Nitric oxide (NO) radical scavenging method. Phytochemical analysis of ethanolic extract of all three plants revealed the presence of flavonoids, alkaloids, saponins, phenolics, carbohydrate, and tannin. The total phenolic, flavonoids and alkaloids content of ethanolic extract of K. galanga, K. parviflora and K, pulchra rhizomes were 0.813, 1.146; 1.047, 1.237, 0.285; 0.755, 0.822, 0.975/100mg respectively. All extracts showed dose dependent free radical scavenging property in the tested models, which was comparable to that of ascorbic acid. The present study concluded that the crude extract of K. galanga, K. parviflora and K, pulchra rhizomes is a rich source of secondary phytoconstituents which impart significant antioxidant potential. It is expected that the important phytochemical properties recognized by our study in the indigenous medicinal plants will be very useful in the curing of various diseases when taken along with our food.

Phytochemical Analysis, In Vitro Antioxidant, and Wound Healing Activities of Turbinaria ornata (Turner) J. Agardh from Gulf of Mannar, India.

Turbinaria ornata (Turner) J., tropical brown algae, was found in the South Pacific and Indian Ocean ecosystems. In accordance with recent studies, Turbinaria ornata J. has potent anti-inflammatory effects. Therefore, this study is aimed to explore the biological activities of ethanolic extract of T. ornata J. by analyzing the presence of phytochemical components, antioxidant property, antimicrobial activity, and the wound healing activity. From the results, phytochemical analysis of ethanolic extract of T. ornata J. showed the presence of alkaloids, saponins, oils, total phenolic, and total flavonoid content which were estimated to be 0.683±0.001 Abs and 0.433±0.001 Abs, respectively. Antioxidant activity of the ethanolic extract of T. ornata J. extract showed remarkable DPPH radical scavenging activity of about 58.8% at 200μg/mL and total antioxidant activity of 0.257 Abs at 100μg/mL concentration, as compared to that of their respective controls. The ethanolic extract of T. ornata J. exhibited the maximum zone of inhibition against the clinical pathogens like Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, and Methicillin-resistant Staphylococcus aureus with their potent antimicrobial activity. Wound healing effects of the ethanolic extract of T. ornata J were analyzed by using zebrafish model. The results showed the rapid and significant regeneration of the wounded caudal fin on day 14. Therefore, the preliminary results of this study strongly support that the ethanolic extract of T. ornata J. may be effective in wound healing and regeneration of the wounded tissues.

Phytochemical analysis of ethanolic extract of leaves of Caesalpinia bonducella

Bioactive chemicals of plant source are phytochemicals (secondary metabolites). They are synthesized naturally in all segments of the plant. Caesalpinia bonducella used for psoriasis care by traditional Siddha physicians in the Malabar regions and is also used in Pakistan's traditional medicine scheme. Tree bark has been identified as a purgative and is recommended for the treatment of disorders of the kapha and vata, gynaecological disorders, skin infections, bowel problems, abdominal distension, piles, ulcers. This study is concerned with the phytoconstituents assessment of the ethanolic extract of Caesalpinia bonducella (EECB) leaves. Method: Using Soxhlet apparatus, coarse powder of Caesalpinia bonducella leaves was extracted with ethanol by hot percolation. For 24 hours, the extraction has been worked out. The solvent was extracted out for acquiring a concentrated extract following extraction. The condensed extract was then vacuum dried and the dry extract was processed for specific bioactive compounds investigation in an air tight container. The existence of alkaloids, carbohydrates, proteins & amino acids, hormones, phenols, tannins, flavonoids, glycosides and saponins was revealed in EECB.

Phytochemical Screening and Analgesic activity study of different solvent fractions of Aerial parts of Operculina turpethum (L.)

Preliminary phytochemical analysis of ethanol extract of the aerial parts of Operculina turpethum L. (Family: Convolvulaceae) exhibited the presence of reducing sugar, phenolic compounds, tannins, flavonoids, carbohydrate, glycosides, alkaloids, acidic compounds, steroids, saponin, and terpenoids. The ethanolic crude extract showed significant (p<0.0001) peripheral analgesic activity at the doses of 250 mg/kg (26.47% writhing inhibition) and 500 mg/kg (50.74% writhing inhibition) determined by acetic acid induced writhing reflex in mice as compared to control Diclofenac sodium (77.94%). After solvent fractionation, n-Hexane, ethyl acetate, butanol and water fraction of Operculina turpethum showed 58.82%, 39.70%, 30.14%, and 20.58% writhing inhibition respectively at the dose of 500 mg/kg body weight. Different phytochemicals present in the extract might be responsible for biological activities found with this study. This research could form the basis of further investigation including pure compounds isolation.

PHYTOCHEMICAL AND GC-MS ANALYSIS OF BIOACTIVE COMPONENTS IN ETHANOLIC EXTRACT OF Rauvolfia vomitoria LEAVES

Herbal medicine has demonstrated great potential of therapeutic benefits in its contribution to modern medicine. Africa is endowed with many plants that can be used for medicinal purposes to which they have taken full advantage. Rauvolfia vomitoria is one of the medicinal plants that is widely distributed across the forests of Africa and Asia. Phytochemical analysis of ethanolic extract of Rauvolfia vomitoria leaves was carried out. The result indicated the presence of Tannins, Saponins, Steroids, Glycosides, Alkaloids, and Flavonoids. Further analysis of the Ethanolic extract of Rauvolfia vomitoria leaves was carried out using GC-MS. Among many compounds determined in the GC-MS analysis of the leaves extract were Methenamine, Benzofuran and Methyl urinate which have been reported to be anti-inflammatory, anti-oxidant, anti-fungal and anti-bacterial. The Ethanolic leave extract of Rauvolfia vomitoria therefore could contribute immensely to effective treatment of some infectious diseases.

Antimicrobial activity, antiproliferative activity, amylase inhibitory activity and phytochemical analysis of ethanol extract of corn (Zea mays L.) silk

In this study, ethanolic extract of Zea mays L was utilized against urinary tract infection (UTI) causing bacteria, vegetable spoilage causing fungi and anticancer activity. The antimicrobial activity of ethanol extracts of corn silk was determined by the agar well diffusion method against UTI pathogens. The most effective antibacterial activity of ethanol corn silk extract was found at 900 ​μg and antifungal activity was found against Aspergillus niger and Aspergillus brasiliensis at 2mg/20 ​ml. It showed 75% nitric oxide inhibition activity and 67%. amylase inhibiting activity. GC-MS analysis showed several bio active compounds with antimicrobial, antioxidant, anticancer activities etc.

Antioxidant Properties and Effect of Ethanolic Extract of Pulcria crispa on Biochemical and Hematological Parameters of Albino Rats

Background: Pulcria crispa (P. crispa) is an herbal plant traditionally used to treat common ailments.
 Objective: In this study, we investigated P. crispa for its phytochemical constituents, antioxidant properties and effects on biochemical and hematological parameters as well as safety in albino rats.
 Methods: Phytochemical analysis of ethanolic extract of P. crispa was conducted using standard procedures. In vitro 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay were used for the investigation of antioxidant activity of extract. Acute effects on physical and behavioral changes and mortality were monitored up to 72 h after administration of different doses of C. crispa Chronic effects on body to organ ratio, biochemical and hematological parameters were measured after administration of rats with different doses of P. crispa extract for 30 days.
 Results: Alkaloids, flavonoids, phenols and tannins were the most abundant constituents found in P. crispa extract, which displayed a significant antioxidant activity measured by reducing power and DPPH assays. No physical, behavioral changes and mortality were noted following the acute treatment of rats with the extract. Similarly, no significant change in body to organ weight ratio was observed after chronic treatment. Hematological parameters including RBCs, Hb, PCV, MCV, MCH and MCHC values were unaltered while W.B.C count was elevated in P.crispa administered rats compared to control.
 
 crispa extract significantly reduced glucose, urea, creatinine, cholesterol, bilirubin, AST and ALT levels, whereas, triglycerides and total protein levels were increased in response to P.crispa treatment.
 
 Conclusions: This study demonstrates that P. crispa extract is rich in bioactive compounds and possesses significant antioxidant properties. Extract was also found to be safe and had no significant adverse effects on hematological parameters and exerted beneficial effects on biochemical parameters.

Quantitative Phytochemical Composition and Bioactive Constituents of Ethanolic Extract of Phyllanthus amarus (schum. et thonn) Leaves

Herbs are used in the treatment of several human ailment and diseases. The quantitative phytochemical composition, bioactive constituents of ethanol extract of Phyllanthus amarus leaves were determined. The quantitative phytochemical analysis of ethanol extract of Phyllanthus amarus found to be present in this study includes phenol, flavonoid, saponin, tannin and alkaloid. The total content of phenol is 73.163µg/ml, flavonoid is 137.27µg/ml, tannin is 3.581µg/ml, alkaloid is 30.1 % and saponin is 34.822 ppm. The GC-MS analysis of bioactive constituents of the ethanol extract of Phyllanthus amarus leaves resulted in 44 compounds which have diverse use. The various bioactive compounds present in P. amarus confirm its uses for the treatment of various ailments and diseases by traditional practitioners.

IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ZIZIPHUS RUGOSA LAM. LEAVES

Ziziphus rugosa Lam. belongs to the family Rhamnaceae and is found chiefly in deciduous and semi evergreen forest of Western Ghats. The present research was undertaken to establish in vitro antioxidant and in vivo hepatoprotective activity of ethanolic extract of Z.rugosa Lam. leaves. The powdered leaves of Z. rugosa were extracted with ethanol and preliminary phytochemical screening was performed for the presence of various phytoconstituents. DPPH assay and β-glucuronidase inhibition assay were selected for the free radical scavenging activity. For the assessment of hepatoprotective activity, alcohol and CCl4 induced hepatotoxicity model were used. The phytochemical analysis of ethanolic extract showed the presence of alkaloids, saponins and flavonoids. The extract exhibited concentration dependent radical scavenging activity with an IC50 value of 61.88 μg/ml and β –glucoronidase inhibition activity with an IC50 value of 70.61 μg/ml. It was speculated that the Z. rugosa Lam. ethanolic extract shows dosedependent hepatoprotective activity which is equivalent with the standard drug Silymarin. The inhibition of free radicals or free radical scavenging activity is significant in the protection against CCl4 and alcohol induced hepatopathy. Hence, it is likely that the antioxidant activity of ethanolic extract of Z. rugosa Lam. might contribute to the hepatoprotective action.

Antioxidant, antimicrobial activity and phytochemical analysis of the seeds extract of Cucumis sativus Linn

Abstract The prehistoric interaction of plants and human is being strengthened by the remarkable use of plants as remedy against diseases. Cucumis sativus L. is extensively cultivated globally including Pakistan. The present study was carried out to find the antioxidant, antimicrobial and phytochemical analysis of Cucumis sativus seed extract. Phytochemical analysis of ethanolic extract revealed the presence of flavonoids, terpenoids, tannins, cardiac glycoside, phenols and carbohydrates. 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used for antioxidant activity. Crude ethanolic extract showed maximum DPPH scavenging activity of 46.05±1.23 at 500µg/ml, while ascorbic acid showed 92.5%. For Antimicrobial activity against selected bacteria and fungi by agar well diffusion method was used. In the antibacterial activity, crude ethanolic extract was most active against Staphylococcus aureus (21.5mm) and less against Shigella flexneri (17.0mm). The n-haxane fraction was highly active against Salmonella typhi (26mm), DCM against E. coli (16.25mm) and Salmonella typhi showed 16.0mm inhibition with ethyl acetate. Crude extract of ethanol was tested against Alternaria, Acremonium, Verticellium, Pythium and Tricoderma sps. Alternaria showed low zone of inhibition (08mm) while the rest of fungi were highly susceptible with zone of inhibition 15mm, 14mm, 17mm and 15mm respectively. The Pythium spp. was highly susceptible to n-haxane fraction (20.00mm), Acremonium to DCM fraction (20.00mm) and to ethyl acetate (16.00 mm). The results showed significant antimicrobial and antioxidant activity which might be because of flavonoids, terpenoids, tannins and phenols in there, suggesting that C. sativus should be used as an active nutrition to control microbial infections leading to chronic diseases. Keywords: Antimicrobial activity; Antioxidant activity; Cucumis sativus L.; Phytochemical analysis http://dx.doi.org/10.19045/bspab.2018.700202

ESTIMATION OF SOME SECONDARY METABOLITES FROM THE IN VITRO CULTURES OF CHLOROPHYTUM BORIVILIANUM SANT. Et. FERN

Objective: To perform phytochemical screening, estimate total saponins and to study the secondary metabolite profiling of Chlorophytum borivilianum by ESI-MS (Electron spray ionisation-mass spectroscopy) and GC-MS (Gas chromatography-mass spectroscopy) techniques.Methods: The powdered samples of leaves and roots (nature grown and freshly harvested and hardened in vitro regenerated) and callus of Chlorophytum borivilianum (2 g) were extracted exhaustively by soxhlet by refluxing with petroleum ether (60-80 °C) and then with ethanol (85%). Alternatively, the methanol extract was prepared for the samples by the cold maceration method and the filtered extract was used. This extract was further analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude extracts. Vanillin sulphuric acid assay was done to estimate the total saponin content of extracts.Results: Phytochemical analysis of ethanolic extract showed the presence of major classes of phytochemicals. The spectrophotometric analysis as well as the GC-MS results revealed the remarkably high percentage of saponin content in the in vitro roots. Besides this, many important secondary metabolites viz. β-Sitosterol and Taraxerone were also revealed by GC-MS.Conclusion: Results of this study showed enhanced production of many important secondary metabolites, especially saponins in tubers of in vitro regenerated plantlets vis a vis that of the nature grown plants establishing the fact that micropropagation can pave new vistas for the research in phytopharmaceuticals and bioprospecting of rare and important medicinal plants.

Phytochemical profile of cansanção nettle extracts and their bioactivities on cabbage caterpillar

Abstract: The objective of this work was to evaluate the phytochemical profile and bioactivity of extracts of Brazilian stinging (“cansanção”) nettle (Cnidoscolus urens) on cabbage caterpillar (Ascia monuste orseis). Mortality, larval stage duration, pupal viability and mass, percentage of deformed pupae, and feeding preference were evaluated. The constituent classes of the ethanolic extracts were also identified by analytical thin-layer chromatography (TLC). Ethanolic extracts of leaves and roots at 2% (m/v) and aqueous extract of roots at 20% (m/v) prolonged the larval phase of the insect. The ethanolic extract of leaves at 2% reduced pupal viability, and pupae from all treatments showed reduced masses and generated deformed adults. Except for the ethanolic extract of stem at 2% (m/v), all other extracts showed a deterrent effect on A. monuste orseis larvae. The phytochemical analysis of ethanolic extracts showed the major presence of phenolic compounds, naphthoquinones, anthraquinones, coumarins, anthracene derivatives, terpenes, and steroids, as well as tannins. Aqueous extracts at 20% concentration and ethanolic extracts at 2% of leaves, stems, and roots of cansanção nettle cause larval mortality and also reduce leaf consumption, pupal viability, and pupal mass of A. monuste orseis.

Botanical and Chemical Fingerprinting of Medicinal Roots of Justicia gendarussa Burm f.

Background:Justicia gendarussa Burm f. of family Acanthaceae is medicinally important herb used in the treatment of inflammatory disorders, asthma, hepatic injuries, pathogenic infection and also shows antiproliferative activity against various cancer cell lines.Materials and Methods:Pharmacognostical evaluation (macro-microscopy, physicochemical analysis and preliminary phytochemical analysis), high-performance thin layer chromatography (HPTLC) fingerprinting and chemical profiling by gas chromatography-mass spectrometry (GCMS) of dried roots of J. gendarussa were done according to quality standard procedures.Results:Microscopic analysis revealed the compact arrangement of cells in cork region and thin-walled cortex beneath epidermis. Parenchymatous cells with xylem vessel were observed in the roots of J. gendarussa. Physicochemical studies revealed loss on drying (10.474%), total ash (2.990%), acid-insoluble ash (0.099%), water-soluble ash (1.528%), alcohol-soluble extractive value (0.564%) and water-soluble extractive value (4.11%) of the raw drug. Preliminary phytochemical analysis of ethanolic extract of J. gendarussa showed the presence of alkaloid, steroid, flavonoid, phenol, carbohydrate, saponin and quinone. Rf, color of the spots and densitometric scan were recorded by HPTLC fingerprinting using toluene: ethyl acetate: formic acid (5.0:4.0:1.0). On photodocumentation, six spots were visualized under 254 nm, nine spots under 360 nm and six spots at 620 nm. Identification of components in ethanolic extract of J. gendarussa was done by GC-MS. GC-MS results in the presence of oleic acid, 9,12-octadecadienoic acid, 6,9,12-octadecatrienoic acid and estra-1, 3,5 (10)-trein-17-β-ol in ethanolic extract of J. gendarussa.Conclusion:These specific identities will be useful in identification and authentication of the raw drug in dried form.SUMMARY Transverse section and powder of dried roots of Justicia gendarussa were examined microscopically. Microscopic observations showed the presence of well-developed cork and cortex. Presence of xylem vessels and parenchymatic rays were observed in transverse section. Parenchymatous cell and sclereids with vessel elements were found in powder microscopyPhysicochemical studies revealed loss on drying (10.474%), total ash (2.990%), acid-insoluble ash (0.099%), water-soluble ash (1.528%), alcohol-soluble extract (0.564%) and water-soluble extract (4.11%)Preliminary phytochemical analysis of ethanolic extract of J. gendarussa showed the presence of alkaloid, steroid, flavonoid, phenol, carbohydrate, saponin and quinoneHigh-performance thin layer chromatography fingerprinting showed different peaks at different wavelengthChemical profiling of medicinal roots of J. gendarussa by gas chromatography. mass spectrometry revealed the presence of oleic acid, 9,12.octadecadienoic acid, 6,9,12-octadecatrienoic acid and estra-1,3,5 (10)-trein-17-β-ol as bioactive compound. Abbreviations Used: TLC: Thin layer chromatography; HPTLC: High performance thin layer chromatography; GCMS: Gas chromatography-mass specrtometry; QSIMP: Quality standard of indian medicinal plant; LOD: Loss on drying; TA: Total ash; AIA: Acid insoluble ash; WSA: Water soluble ash; ASE: Alcohol soluble extractive; WSE: Water soluble extractive.