Physiological Map Research Articles

P05-50 Using ontology map and physiological map to improve predictive models for kidney toxicity

P05-50 Using ontology map and physiological map to improve predictive models for kidney toxicity

Enhancing traumatic brain injury emergency care: the impact of grading and zoning nursing management

ABSTRACT Objective This research aimed to evaluate the impact of grading and zoning nursing management on traumatic brain injury (TBI) patients’ emergency treatment outcomes. Methods This randomized controlled trial included 200 TBI patients. They were treated with a conventional care (control group, n = 100) and a novel grading and zoning approach (study group, n = 100), respectively. This innovative model organized care into levels based on urgency and complexity, facilitating targeted medical response and resource allocation. Key metrics compared included demographic profiles, consultation efficiency (time metrics and emergency treatment rates), physiological parameters (HR, RR, MAP, SpO2, RBS), and patient outcomes (hospital and ICU stays, complication rates, and emergency outcomes). Results The study group demonstrated significantly improved consultation efficiency, with reduced times for physician visits, examinations, emergency stays, and specialist referrals (all p < 0.001), alongside a higher emergency treatment rate (93% vs. 79%, p = 0.004), notably better physiological stability, improved HR, RR, MAP, SpO2 and RBS (p < 0.001), shorter hospital and ICU stays, fewer complications, and superior emergency outcomes. Conclusion Grading and zoning nursing management substantially enhances TBI patients’ emergency care efficiency and clinical outcomes, suggesting a viable model for improving emergency treatment protocols.

P06-36: The nephron physiological map as a tool to build kidney disease ontologies

P06-36: The nephron physiological map as a tool to build kidney disease ontologies

Pre‐stenting angiography‐FFR based physiological map provides virtual intervention and predicts physiological and clinical outcomes

Angiography-derived fractional flow reserve (FFR) (angio-FFR) has been validated against FFR and could provide virtual pullback. However, whether a physiological map can be generated by angio-FFR and its clinical value remains unclear. We aimed to investigate the feasibility of physiological map created from angio-FFR pullback and its value in predicting physiological and clinical outcomes after stenting. An angio-FFR physiological map was generated by overlaying the virtual pullback onto coronary angiogram, to calculate physiological stenosis severity, length, and intensity (Δangio-FFR/mm). This map in combination with virtual stenting was used to predict the best-case post-percutaneous coronary intervention (PCI) angio-FFR (angio-FFRpredicted ) according to the stented segments, and this was compared with the actual achieved post-PCI angio-FFR (angio-FFRachieved ). Additionally, prognostic value of predicted angio-FFR was investigated. Three hundred twenty-nine vessels with paired analyzable pre- and post-PCI angio-FFR were included. Physiological map was created successfully in all vessels. After successful PCI, angio-FFRpredicted and angio-FFRachieved were significantly correlated (r = 0.82, p < 0.001) with small difference (mean difference: -0.010 ± 0.035). In the virtual PCI only covering the segment with high angio-FFR intensity, the same physiological outcome can be achieved with shorter stent length (14.1 ± 8.9vs. 34.5 ± 15.8 mm, p < 0.001). Suboptimal angio-FFRpredicted was associated with increased risk of 2-year vessel-oriented composite endpoint (adjusted hazard ratio: 3.71; 95% confidence interval: 1.50-9.17). Angio-FFR pullback could provide a physiological map of the interrogated coronary vessels by integrating angio-FFR pullback and angiography. Before a PCI, the physiological map can predict the physiological and clinical outcomes after stenting.

Structural and functional organization of the lower jaw barrel subfield in rat primary somatosensory cortex.

Barrel subfields in rodent primary somatosensory cortex (SI) are important model systems for studying cortical organization and reorganization. During cortical reorganization that follows limb deafferentation, neurons in deafferented forelimb SI become responsive to previously unexpressed inputs from the lower jaw. Although the lower jaw barrel subfield (LJBSF) is a likely source of the input, this subfield has received little attention. Our aim was to describe the structural and functional organization of the normal LJBSF. To investigate LJBSF organization, a nomenclature for lower jaw skin surface was developed, cytochrome oxidase (CO) was used to label flattened-cut LJBSF sections, microelectrodes were used to map the lower jaw skin surface representation in SI, and electrolytic lesions, recovered from electrode penetrations, were used to align the physiological map to the underlying barrel map. LJBSF is a tear-shaped subfield containing approximately 24 barrels, arranged in eight mediolateral rows and a barrel-free zone capping the anterior border. The representation of the lower jaw skin consisting of chin vibrissae and microvibrissae embedded in common fur is somatotopically organized in a single map in the contralateral SI. This physiological map shows that the activity from the vibrissae aligns with the CO-staining of the underlying LJBSF. LJBSF barrels receive topographically ordered barrel-specific input from individual vibrissa and microvibrissae in the lower jaw but not from trident whiskers. The barrel-free zone receives topographically ordered input from the lower lip. These data demonstrating that the LJBSF is a highly organized subfield are essential for understanding its possible role in cortical reorganization.

Fuzzy Torque Control of the Bionic Flexible Manipulator Actuated by Pneumatic Muscle Actuators

A bionic flexible manipulator driven by pneumatic muscle actuator (PMA) can better reflect the flexibility of the mechanism. Current research on PMA mainly focuses on the modeling and control strategy of the pneumatic manipulator system. Compared with traditional electro-hydraulic actuators, the structure of PMA is simple but possesses strong nonlinearity and flexibility, which leads to the difficulty in improving the control accuracy. In this paper, the configuration design of a bionic flexible manipulator is performed by human physiological map, the kinematic model of the mechanism is established, and the dynamics is analyzed by Lagrange method. A fuzzy torque control algorithm is designed based on the computed torque method, where the fuzzy control theory is applied. The hardware experimental system is established. Through the co-simulation contrast test on MATLAB and ADAMS, it is found that the fuzzy torque control algorithm has better tracking performance and higher tracking accuracy than the computed torque method, and is applied to the entity control test. The experimental results show that the fuzzy torque algorithm can better control the trajectory tracking movement of the bionic flexible manipulator. This research proposes a fuzzy torque control algorithm which can compensate the error more effectively, and possesses the preferred trajectory tracking performance.

Analysis of essential gene dynamics under antibiotic stress in Streptococcus sanguinis.

The paradoxical response of Streptococcus sanguinis to drugs prescribed for dental and clinical practices has complicated treatment guidelines and raised the need for further investigation. We conducted a high throughput study on concomitant transcriptome and proteome dynamics in a time course to assess S. sanguinis behaviour under a sub-inhibitory concentration of ampicillin. Temporal changes at the transcriptome and proteome level were monitored to cover essential genes and proteins over a physiological map of intricate pathways. Our findings revealed that translation was the functional category in S. sanguinis that was most enriched in essential proteins. Moreover, essential proteins in this category demonstrated the greatest conservation across 2774 bacterial proteomes, in comparison to other essential functional categories like cell wall biosynthesis and energy production. In comparison to non-essential proteins, essential proteins were less likely to contain ‘degradation-prone’ amino acids at their N-terminal position, suggesting a longer half-life. Despite the ampicillin-induced stress, the transcriptional up-regulation of amino acid-tRNA synthetases and proteomic elevation of amino acid biosynthesis enzymes favoured the enriched components of essential proteins revealing ‘proteomic signatures’ that can be used to bridge the genotype–phenotype gap of S. sanguinis under ampicillin stress. Furthermore, we identified a significant correlation between the levels of mRNA and protein for essential genes and detected essential protein-enriched pathways differentially regulated through a persistent stress response pattern at late time points. We propose that the current findings will help characterize a bacterial model to study the dynamics of essential genes and proteins under clinically relevant stress conditions.

The Reconstruction and Analysis of Gene Regulatory Networks.

In post-genomic era, an important task is to explore the function of individual biological molecules (i.e., gene, noncoding RNA, protein, metabolite) and their organization in living cells. For this end, gene regulatory networks (GRNs) are constructed to show relationship between biological molecules, in which the vertices of network denote biological molecules and the edges of network present connection between nodes (Strogatz, Nature 410:268-276, 2001; Bray, Science 301:1864-1865, 2003). Biologists can understand not only the function of biological molecules but also the organization of components of living cells through interpreting the GRNs, since a gene regulatory network is a comprehensively physiological map of living cells and reflects influence of genetic and epigenetic factors (Strogatz, Nature 410:268-276, 2001; Bray, Science 301:1864-1865, 2003). In this paper, we will review the inference methods of GRN reconstruction and analysis approaches of network structure. As a powerful tool for studying complex diseases and biological processes, the applications of the network method in pathway analysis and disease gene identification will be introduced.

106 Virtual-PCI With iFR-Pullback can Plan Coronary Intervention in Complex Coronary Disease with a Prediction of Functional Gain

Background Coronary intervention is increasingly performed in complex and tandem coronary disease where physiological optimisation is less practical. Advances in computation allow physiological maps of stenosis-specific coronary pressure loss to be mapped upon angiographic images. Virtual-PCI conceptually allows selective removal of a stenosis and predict the functional gain of a given stenting strategy. We sought to assess the change in resting haemodynamics after intervention and the feasibility of Virtual-PCI to predict post-PCI iFR. Method Pressure and flow velocity measurements were made before and after PCI to 75 stenoses. Data was used to model the change in pressure ratios after PCI. Intracoronary motorised pressure-wire iFR-pullbacks were performed at rest in a separate cohort of 32 coronary arteries with tandem and diffuse disease to iFR changes onto the angiogram. Virtual-PCI predicted a post-PCI iFR (iFR predicted ) assuming the perfect removal of a given stenosis (Figure 1). This was compared to the observed iFR post real-world PCI (iFR observed ). Results Resting flow velocity over the wave-free period changed by only 4.4 cm/s; a demonstrable change was noted when stenoses had pre-PCI FFR expected of 0.94 ± 0.01. The observed iFR after real-world PCI was iFR observed 0.93 ± 0.01 with no significant difference between the predicted and observed increase (ΔiFR expected :0.16 ± 0.03 vs ΔiFR observed 0.13 ± 0.03 p = 0.48). No significant systematic bias between iFR expected and iFR observed was noted (Bland-Altman mean difference 0.016 ± 0.004). Conclusions Virtual-PCI enables selective removal a stenosis from an iFR-pullback physiological map of the vessel and predict the haemodynamic effect of that chosen intervention. The post-PCI physiological result can be predicted without systematic bias permitting virtual assessment of the functional gain of different stenting strategies. Virtual-PCI using iFR-Pullback is feasible in the catheter laboratory and real-time algorithms may enable live co-registration with the angiogram to make an advance in how intervention is performed in the future.

Developing a High-Resolution Physiological Equivalent Temperature Map to Assess the Spatial Variation of Thermal Stress Exposure for an Elderly Cohort of Chinese in Hong Kong

Conference Theme: From Local to Global: Advancing Science for Policy in Environmental Health!

Using Heart Failure with Preserved Ejection Fraction to Understand an ‘Omics Approach to Evaluating Vascular Dysfunction and Cardiovascular Disease

Regulation of vascular tone is complex due to the presence of redundant mechanisms operating over multiple spatial domains (cell, tissue and organ) and influenced by various confounding factors, such as sex, age and time. As a result, the task of identifying the underlying mechanisms of vascular dysfunction associated with complex cardiovascular diseases, like heart failure with preserved ejection fraction (HFpEF), remains under explored. ‘Omics research strategies are proposed to be an effective way to navigate complicated physiology. In this review an ‘omics approach is applied to the spatial domains of HFpEF using a mechanistic “physiological map” of factors known to be associated with vascular (dys) function. The result is intended to generate hypotheses, foster robust study design, and highlight the necessity for collaborative, interdisciplinary approaches to conduct ‘omics research, which in turn is expected to facilitate the development of efficacious therapies for cardiovascular diseases such as HFpEF, where etiologies are unclear and evidence-based therapeutic strategies lacking.

A physiological frequency-position map of the chinchilla cochlea

Accumulating evidence indicates that mammalian cochlear frequency-position maps (location of maximum vibration of the basilar membrane as a function of frequency) depend on the physiological condition of the inner ear. Cochlear damage desensitizes the ear, after the damage the original location of maximum vibration is tuned to a lower sound frequency. This suggests that frequency-position maps, derived from such desensitized ears, are shifted to lower frequencies, corresponding to a shift of the basilar membrane vibration pattern towards the base for a given stimulus frequency. To test this hypothesis, we re-mapped the cochlear frequency-position map in the chinchilla. We collected frequency-position data from chinchillas in normal physiological condition (“physiological map”) and compared these to data previously established from sound overexposed ears (“anatomical map”). The characteristic frequency (CF) of neurons in the cochlear nucleus was determined. Horse-radish peroxidase (HRP) or biocytin (BCT) were injected iontophoretically to trace auditory nerve fibers towards their innervation site in the organ of Corti. The relationship between distance from the base ( d, percent) and frequency ( f, kHz) was described best by a simple exponential function: d = 61.2 − 42.2 × log( f). The slope of the function was 2.55 mm/octave. Compared to the “anatomical map”, the “physiological map” was shifted by about 0.3 octaves to higher frequencies corresponding to a shift of the basilar membrane vibration pattern of 0.8 mm towards the apex for a given stimulus frequency. Our findings affirm that frequency-position maps in the mammalian cochlea depend on the condition of the inner ear. Damage-induced desensitization in mammalian inner ears results in similar shifts of CF (about 0.5 octaves) but different shifts of the maximum of the vibration pattern towards the base at given frequencies, dependent on the mapping constant of the species, longer basilar membranes showing a larger basal shift. Furthermore, the results substantiate the notion that “crowding” at lower frequencies appears to be a specialization rather than a general feature.

Shift in the cochlear place???frequency map after noise damage in the mouse

The cochlear place-frequency map, determined from noise-damaged mice, is shifted to lower frequencies by up to one octave compared with the map determined from normal hearing mice. To test the hypothesis that the shift results from damage to the cochlear amplifier, we measured frequency tuning curves from the same neurons before and after noise exposure. Noise damage resulted in loss of tuning and elevation of thresholds. The neuronal characteristic frequency shifted by 0.6-1.2 octaves, dependent on frequency. The shift in characteristic frequency was used to calculate a shifted place-frequency map. We conclude that desensitization of areas in the inner ear after noise exposure can explain the shift of the map after noise damage relative to the normal physiological map.

Olfactory inputs activate the medial entorhinal cortex via the hippocampus.

The lateral and medial regions of the entorhinal cortex differ substantially in terms of connectivity and pattern of activation. With regard to olfactory input, a detailed and extensive physiological map of the olfactory projection to the entorhinal cortex is missing, even if anatomic studies suggest that the olfactory afferents are confined to the lateral and rostral entorhinal region. We studied the contribution of the medial and lateral entorhinal areas to olfactory processing by analyzing the responses induced by lateral olfactory tract stimulation in different entorhinal subfields of the in vitro isolated guinea pig brain. The pattern of synaptic activation of the medial and lateral entorhinal regions was reconstructed either by performing simultaneous multisite recordings or by applying current source density analysis on field potential laminar profiles obtained with 16-channel silicon probes. Current source density analysis demonstrated the existence of a direct monosynaptic olfactory input into the superficial 300 microm of the most rostral part of the lateral entorhinal cortex exclusively, whereas disynaptic sinks mediated by associative fibers arising from the piriform cortex were observed at 100-350 microm depth in the entire lateral aspect of the cortex. No local field responses were recorded in the medial entorhinal region unless a large population spike was generated in the hippocampus (dentate gyrus and CA1 region) by a stimulus 3-5x the intensity necessary to obtain a maximal monosynaptic response in the piriform cortex. In these conditions, a late sink was recorded at a depth of 600-1000 microm in the medial entorhinal area (layers III-V) 10.6 +/- 0.9 (SD) msec after a population spike was simultaneously recorded in CA1. Diffuse activation of the medial entorhinal region was also obtained by repetitive low-intensity stimulation of the lateral olfactory tract at 2-8 Hz. Higher or lower stimulation frequencies did not induce hippocampal-medial entorhinal cortex activation. These results suggest that the medial and the lateral entorhinal regions have substantially different roles in processing olfactory sensory inputs.

Effects of large-scale limb deafferentation on the morphological and physiological organization of the forepaw barrel subfield (FBS) in somatosensory cortex (SI) in adult and neonatal rats.

The physiological representation of the shoulder and surrounding body was examined in layer IV of somatosensory cortex (SI) in rats that had underground removal of the forelimb, either as newborns on postnatal day three (PND-3) or as adults (at least 8 weeks of age). Electrophysiological recordings were used to map the shoulder and body representations (physiological map), and the mitochondria marker, cytochrome oxidase (CO), was used to visualize recording sites in barrel and barrel-like structures (morphological map) in layer IV of deafferents and intact controls. The SI shoulder representation lies in a nebulously stained region that lies posterior to the forearm, wrist, and forepaw representations; the latter region is associated with the well-defined forepaw barrel subfield (FBS). The major findings are: (1) the shoulder is represented as a single zone located at the posterior extent of the SI body map in intact rats; (2) limb deafferentation in adult or neonatal rats that were physiologically mapped 6-16 weeks post-amputation resulted in two or more islets of "new" representation of the shoulder in the FBS in addition to the representation of the "original" shoulder in the posterior part of the body map; (3) deafferentations made in neonatal rats, physiologically mapped as adults, had a significantly greater (Mann-Whitney U) amount of "new" cortical representation within the FBS than did rats deafferented as adults; (4) fewer unresponsive sites in the FBS were found for neonate deafferents than for adult deafferents; (5) evoked response latencies following electrical stimulation of the shoulder were shortest for cortical sites within the "original" shoulder representation in intact controls, and latencies recorded at the "original" shoulder representation in deafferents were also shorter than latencies recorded in "new" shoulder representations in both groups of deafferents; and (6) morphological maps of the FBS were altered in neonate deafferents to the extent that the barrel structure was poorly formed, as exemplified by the absence of the four mediolateral running bands; however, the overall ovoid shape of the FBS was still apparent, but not as sharply defined as for intact controls or adult deafferents. Possible mechanisms for reorganization following large-scale deafferentation are discussed.

Visualization of the information flow through human oculomotor cortical regions by transcranial magnetic stimulation.

We investigated the topography of human cortical activation during an antisaccade task by focal transcranial magnetic stimulation (TMS). We used a figure-eight shaped coil, with the stimulus intensity set just above the threshold for activation of the hand motor areas but weak enough not to elicit blinks. TMS was delivered at various time intervals (80, 100, and 120 ms) after target presentation over various sites on the scalp while the subjects performed the antisaccade task. It was possible to elicit a mild but significant delay in saccade onset over 1) the frontal regions (a region 2-4 cm anterior and 2-4 cm lateral to hand motor area) and 2) posterior parietal regions (6-8 cm posterior and 0-4 cm lateral to hand motor area) regardless of which hemisphere was stimulated. The frontal regions were assumed to correspond to a cortical region including the frontal eye fields (FEFs), whereas the parietal regions were assumed to represent a wide region that includes the posterior parietal cortices (PPCs). The regions inducing the delay shifted from the posterior parietal regions at an earlier interval (80 ms) to the frontal regions at a later interval (100 ms), which suggested an information flow from posterior to anterior cortical regions during the presaccadic period. At 120 ms, the effect of TMS over the frontal regions still persisted but was greatly diminished. Erroneous prosaccades to the presented target were elicited over a wide cortical region including the frontal and posterior parietal regions, which again showed a forward shift with time. However, the distribution of effective regions exhibited a clear contralateral predominance in terms of saccade direction. Our technique provides a useful method not only for detecting the topography of cortical regions active during saccadic eye movement, but also for constructing a physiological map to visualize the temporal evolution of functional activities in the relevant cortical regions.

Microelectrode-guided pallidotomy: technical approach and its application in medically intractable Parkinson's disease.

The authors describe the microelectrode recording and stimulation techniques used for localizing the caudal sensorimotor portion of the globus pallidus internus (GPi) and nearby structures (internal capsule and optic tract) in patients undergoing GPi pallidotomy. Localization is achieved by developing a topographic map of the abovementioned structures based on the physiological characteristics of neurons in the basal ganglia and the microexcitable properties of the internal capsule and optic tract. The location of the caudal GPi can be determined by "form fitting" the physiological map on relevant planes of a stereotactic atlas. A sensorimotor map can be developed by assessing neuronal responses to passive manipulation or active movement of the limbs and orofacial structures. The internal capsule and optic tract, respectively, can be identified by the presence of stimulation-evoked movement or the patient's report of flashes or speckles of light that occur coincident with stimulation. The optic tract may also be located by identifying the neural response to flashes of light. The anatomical/physiological map is used to guide lesion placement within the sensorimotor portion of the pallidum while sparing nearby structures, for example, the external globus pallidus, nucleus basalis, optic tract, and internal capsule. The lesion location and size predicted by using physiological recording together with thin-slice high-resolution magnetic resonance imaging reconstructions of the lesion were confirmed in one patient on histological studies. These data provide important information concerning target identification for ablative or deep brain stimulation procedures in idiopathic Parkinson's disease and other movement disorders.

A histologically visible representation of the fingers and palm in primate area 3b and its immutability following long-term deafferentations.

An isomorph of the glabrous hand is visible in primary somato-sensory cortex (area 3b) of owl monkeys in brain sections cut parallel to the surface and stained for myelin. A mediolateral row of five ovals, separated by myelin-light septa, represents digits and corresponds precisely with cortical sites activated by light touch on individual digits in microelectrode recordings. A number of caudal ovals relate to pads of the palm. A more distinct septum separates the hand from the more lateral face representation. Within the face representation, two large myelin-dense ovals can be identified that are activated by the upper or lower face in a caudo-rostral sequence. Accidental finger loss or dorsal column section, deafferentations that result in reorganization of the physiological map in area 3b, do not alter the morphological map. The proportions for each digit and palm in the morphological map do not vary across normal and deafferented animals. Similar isomorphs were also seen in area 3b of squirrel and macaque monkeys. We conclude that the anatomical isomorph for the body surface representation in area 3b is a reliable reflection of normal cortical organization and may be a common feature of the primate area 3b. The isomorph can provide a reference in studies of somatotopic reorganization.

Commentary on high-performance computing and its promises for the behavioral sciences

Even with the advent of magnetic resonance imaging and other sophisticated physiological research, we have not made a great leap forward in creating a physiological map relating each neuron to its activity involved in simple day-to-day psychological processes. In contrast to notable progress in human vision research, we have yet to model the simplest of our daily memory processes in memory research.

Digit removal leads to discrepancies between the structural and functional organization of the forepaw barrel subfield in layer IV of rat primary somatosensory cortex.

The physiological representation of the forepaw in rat primary somatosensory cortex (SI) is topographically organized. This representation is associated with the unique arrangement of barrels in layer IV of the forepaw barrel subfield (FBS) in SI and provides an example of a relationship between cortical structure and function. It has been reported that removal of peripheral afferent input to the FBS prior to postnatal day 5 or 6 results in a disorganized FBS, while deafferentation at later times produces little or no alteration of the FBS. Therefore, restricted deafferentations of individual digits in adult rats should result in little, if any, disruption of the FBS, while at the same time eliminating afferent input to the FBS from a localized region of the periphery. This manipulation is likely to create a mismatch between structure and function and offer insight into what barrels actually represent in the adult deafferent. In the present study, we amputated digit three (D3) in eight adult rats, allowed a 1-month survival time, physiologically mapped the representation of D2, D4, and the stump, and compared this physiological map to the underlying barrels in the FBS. Our results showed that FBS barrels formerly associated with the representation of D3 were now associated with the representation of surrounding digits D2 and D4, as well as the remaining stump. By superimposing the morphological and physiological map upon one another, it was clear that the D2 and D4 representations expanded into the former D3 barrel territory and septae between the barrels. The reorganized physiological map was somatotopically organized, even though the general configuration of the morphological map remained unaltered, as visualized with cytochrome oxidase staining. These results suggest that in the deafferent, neurons within FBS barrels previously associated with the representation of punctate regions of skin become associated with neighboring regions of skin. A morphological substrate to account for this cortical reorganization is described.