Physical Stress Research Articles

Role of microglia in stress-induced alcohol intake in female and male mice.

Rates of alcohol use disorder (AUD) have escalated in recent years, with a particular increase among women. Women are more susceptible to stress-induced alcohol drinking, and preclinical data suggest that stress can increase alcohol intake in female rodents; however, a comprehensive understanding of sex-specific neurobiological substrates underlying this phenomenon is still emerging. Microglia, the resident macrophages of the brain, are essential for reshaping neuronal processes, and microglial activity contributes to overall neuronal plasticity. We investigated microglial dynamics and morphology in limbic brain structures of male and female mice following exposure to stress, alcohol or both challenges. In a modified paradigm of intermittent binge drinking (repeated "drinking in the dark"), we determined that female, but not male, mice increased their alcohol consumption after exposure to a physical stressor and re-exposure trials in the stress-paired context. Ethanol (EtOH) drinking and stress altered a number of microglial parameters, including overall number, in subregions of the amygdala and hippocampus, with effects that were somewhat more pronounced in female mice. We used the CSF1R antagonist PLX3397 to deplete microglia in female mice to determine whether microglia contribute to stress-induced escalation of EtOH intake. We observed that microglial depletion attenuated stress-induced alcohol intake with no effect in the unstressed group. These findings suggest that microglial activity can contribute to alcohol intake under stressful conditions, and highlight the importance of evaluating sex-specific mechanisms that could result in tailored interventions for AUD in women.

Schmorl's nodes in a historic adult skeletal sample (19th to 20th centuries): An analysis of age, sex and occupation

ObjectiveThis study explores the interplay between age-at-death, sex and occupation and the presence, location and severity of Schmorl’s nodes. MaterialsVertebral columns of 327 individuals, 180 (55.1%) males and 147 (44.9%) females, with age-at-death between 20 and 65 years old, with known occupation. MethodsSchmorl’s nodes were recorded as present/absent and by location and severity. ResultsIn this sample, 58.7% (192/327) of individuals were affected by Schmorl’s nodes, 75.6% (136/180) were males and 38.1% (56/147) were females, with statistically significant differences (p=0.000). Schmorl’s nodes were most commonly found on the T7-L2 (77.1% of all Schmorl’s nodes) vertebrae and at the center (73.4%) of the vertebral body surface. Age and occupational categories did not correlate with prevalence, quantity or severity. ConclusionsMales appear more prone to develop Schmorl's nodes than females. In this study, the prevalence of Schmorl’s nodes does not increase with age, nor with the type of occupation held by males. SignificanceThis study rejects the purported associations between prevalence of Schmorl’s nodes and age and physical stress. LimitationsIt is unknown whether individuals had the same occupation throughout their lives or for how long they performed it. Additionally, it is impossible to access when the individual developed the Schmorl’s node. Suggestions for further researchEvaluate the onset of Schmorl’s nodes in individuals under 20 and explore possible links between vertebral morphology and the occurrence of Schmorl’s nodes.

Cardiac Adaptation in Power Athletes: Differential Impact of Judo and Weightlifting.

Background: According to the ESC guidelines, sport disciplines are classified in relation to the predominant component (skill, power, mixed and endurance), including a wide range of disciplines with different isometric/isotonic exercises and exercise-induced heart remodeling. The aim of our study was to evaluate differences in morpho-functional cardiac adaptations in power athletes, comparing judokas with weightlifters. Methods: We enrolled 55 Olympic athletes (38 judokas, 17 weightlifters), aged 24.5 ± 3.8 years, 25 (45.4%) of whom were males, and they underwent a pre-participation evaluation, including a physical examination, ECG, transthoracic echocardiogram, and exercise stress test. Results: The judokas presented significant differences in cardiac adaptations, with larger left ventricle (LV) end-diastolic and end-systolic volumes indexed (LVEDVi, p = 0.002 and LVESVi, p = 0.004) and higher LVMass values indexed (p = 0.033), but similar LV wall thicknesses (p = 0.093) and LV ejection fractions (p = 0.981). Also, the left atrium (LA) dimension (p = 0.0002) and volume indexed (p < 0.0001) were higher in the judokas, as were the larger right ventricle (RV) areas. Finally, the judokas showed higher VO2max (p = 0.012), O2 pulse (p = 0.007), VE/O2 LT1 (p = 0.041) and VE/O2 LT2 (p = 0.036) values, with a lower resting heart rate (p = 0.031) and higher exercise capacity (p = 0.011). Conclusions: The judokas showed substantial differences in cardiac morpho-functional adaptations from the weightlifters, and, accordingly, judo should be more properly considered not a pure strength sport but more similar to mixed disciplines of the ESC classification.

Factors associated with intrinsic capacity impairment in hospitalized older adults: a latent class analysis

BackgroundIntrinsic capacity (IC) is proposed by the World Health Organization (WHO) to promote healthy aging. Although some studies have examined the factors influencing IC, few studies have comprehensively confirmed lifestyle factors on IC, especially IC impairment patterns. The present study aimed to identify the patterns of IC impairment and explore the lifestyle and other factors associated with different patterns of IC impairment.MethodsThis cross-sectional study was conducted in a Chinese geriatric hospital. IC was evaluated in five domains according to the recommendations of WHO: cognition, locomotion, vitality, sensory and psychological domains. The sociodemographic and health-related characteristics of participants were assessed.The health promoting lifestyle was evaluated using the Health-Promoting Lifestyle Profile-II scale, including nutrition, health responsibility, interpersonal relationships, physical activity, spiritual growth and stress management. We applied latent class analysis to identify IC impairment patterns and compared basic activities of daily living, instrumental activities of daily living, frailty, quality of life and falls among different IC impairment patterns. Multinomial logistic regression analysis was conducted to identify factors influencing the IC impairment patterns.ResultsAmong 237 participants included, the latent class analysis identified three patterns of IC impairment: 44.7% high IC (Class 1), 31.2% intermediate IC mainly locomotor impairment (Class 2) and 24.1% low IC mainly cognitive impairment (Class 3). Older adults in class 1 had the best function ability and quality of life, while class 3 had the highest levels of disability and frailty, the poorest quality of life and a higher prevalence of falls. Compared with class 1, older adults with advanced age (OR = 22.046, 95%CI:1.735-280.149), osteoporosis (OR = 3.377, 95%CI:1.161–9.825), and lower scores in physical activity (OR = 0.842, 95%CI:0.749–0.945), stress management (OR = 0.762, 95%CI:0.585–0.993) and social support (OR = 0.897, 95%CI:0.833–0.965) were more likely to belong to the class 2. Simultaneously, compared with class 1, older adults with advanced age (OR = 104.435, 95%CI:6.038-1806.410), stroke (OR = 3.877, 95%CI:1.172–12.823) and lower scores in physical activity (OR = 0.784, 95%CI:0.667–0.922) and social support (OR = 0.909, 95%CI:0.828–0.998) were more likely to be class 3. In addition, compared with class 2, older adults with a lower score in nutrition (OR = 0.764, 95%CI:0.615–0.950) were more likely to belong to the class 3.ConclusionsThis study provides evidence that there are heterogeneous IC impairment patterns in older adults and identifies various associated factors in each pattern, including age, stroke, osteoporosis, social support and lifestyle behaviors such as nutrition, physical activity and stress management. It informs stakeholders on which modifiable factors should be targeted through public health policy or early intervention to promote IC and healthy aging in older adults.

Anti‐Blackness and psychological stress: The application of critical race psychology and minority stress theory among Black communities

AbstractIncreased anti‐Blackness, including interpersonal racist encounters and macro forms such as extrajudicial killings by the state, have demonstrated that Black communities and their psychological well‐being are in a precarious position. Extant research has revealed that increased encounters with anti‐Blackness are related to excessive psychological stress. However, the role of stigma awareness, its relationship to anti‐Blackness and psychological stress, and our understanding of the relationship among subgroup identities (e.g., gender) remain underdeveloped. Appropriately, the current work adopted critical race psychology and minority stress theory and explored Black individuals’ (N = 410) experience with anti‐Blackness, stigma awareness, and their relationship to psychological stress. Results demonstrated that experience with anti‐Blackness was positively associated with increased physical, emotional, and cognitive stress; however, stigma awareness mediated only emotional stress. In addition, among the sample, Black men and Black individuals with increased education (e.g., received a college degree) reported increased levels of psychological stress compared to Black women and less educated Black individuals.

Evaluation of Healthcare Workers Attitudes Toward Handling and Treating COVID-19 Patients in Diyala Governorate, Iraq

Background: In 2019, the World Health Organization (WHO) declared a global state of emergency due to the spread of the COVID-19 pandemic. Objective: This study aimed to determine the attitudes of healthcare workers in various Iraqi health institutions towards treating patients with COVID-19. Additionally, it aimed to identify the most important reasons behind preventing them from doing their job. Methods: Between Nov. 2021 to Dec. 2022, we conducted a self-administered cross-sectional survey of 207 healthcare worker participants. The data were collected using a questionnaire that included three parts. The first part included sociodemographic data, the second part aimed to assess the participants’ information concerning how they dealt with this pandemic, and the third part was designed to identify the participants’ attitudes. The statistic data were analysed by using (SPSS), 25.0/ IBM Crop., Armonk, NY. The scientific content of the questionnaire items was taken from the online literature as well as updated information on the WHO website. Results: The results study revealed that 135 (65.22\%) of the participants were willing to receive and treat COVID-19 patients in hospitals and private clinics. While 72 (34.78\%) of the study participants showed their unwillingness to treat infected patients. The most crucial reasons behind their reluctance were the fear of transmission of infection and the lack of protective equipment. Conclusion: The study concluded that the participants' willingness was good. This was indicated by their positive attitudes toward carrying out their medical duties and commitment to providing health care and treatment for COVID-19 patients. There is an urgent need to reduce psychological and physical stress as well as the pressures that health workers suffer from because they are facing large numbers of daily infections. This reduction is achieved via training, continuous education and equipping hospitals with the latest protective supplies to confront epidemics and pandemics.

Stress reduction through taiji: a systematic review and meta-analysis

PurposeIn light of the mounting prevalence of stress in contemporary society and the growing interest in stress reduction methods, this review investigates the potential of taiji as a viable strategy for alleviating stress.MethodsMEDLINE, EMBASE, the Cochrane Controlled Trials Register (CENTRAL), PsycINFO, and Web of Science were searched up to April 2023 to identify randomized controlled trials of taiji. Studies in both patients and healthy populations were considered. They had to provide a measure of perceived stress and include a no treatment or placebo control group. Data were extracted by two reviewers. Pooled standardized mean differences (SMD) were calculated for perceived stress, biological stress markers, anxiety, depression, and quality of life (QoL). Meta-regression analyses were performed to identify sources of heterogeneity.ResultsEleven trials with a total of 1323 patients comparing taiji to no intervention met the inclusion criteria. The included studies varied strongly with regard to patient characteristics, taiji intervention, and methodological quality. The overall SMD for perceived stress was significant at -0.41 (95% confidence interval, CI, -0.63 to -0.19; I2 = 63%). Exclusion of studies with less than 100 participants yielded a diminished SMD at -0.26 (95% CI, -0.45 to -0.06). The SMD for perceived stress at follow-up was significant (-0.25, 95% CI -0.46 to -0.05). Secondary outcomes highlighted improvements in anxiety and physical QoL, while depression, mental QoL, and biological stress markers remained unchanged.ConclusionsResults underscore taiji's potential in mitigating perceived stress in both patients and healthy populations, paralleled by enhancements in depressive symptoms, anxiety levels, and physical QoL.

Improving staff wellness via an after-hours healthy sustainable meals program: A general surgery residency pilot.

Healthcare worker wellness is foundational to delivering quality care. Yet, healthcare facilities often lack access to healthy and sustainable food overnight and on weekends. Healthy, low-carbon meals were provided free of charge after hours to on-call General Surgery residents at the University of British Columbia and the impact on resident well-being assessed using pre- and post-intervention surveys. Financial and time stress reduced significantly with the provision of meals (P's < .01), while emotional and physical stress levels did not change. Average meal expenses decreased from $33 to $10 (P < .001). Increasing food access on call is an impactful intervention to improve resident health and well-being.

Noradrenergic Regulation of the Medial Prefrontal Cortex Mediates Stress Coping in Postpartum Female Mice.

The prevalence of depression in women increases during the postpartum period. We previously reported that subchronic exposure to social stress decreased passive coping in postpartum female mice. This study aimed to investigate whether noradrenaline regulation might regulate coping styles in mice. We first determined whether a different type of stress, subchronic physical stress, decreases passive coping in postpartum females. Postpartum female, virgin female, and male mice were exposed to subchronic restraint stress (restraint stress for 4h for 5 consecutive days). Subchronic restraint stress decreased passive coping in postpartum females but not in virgin females and males in the forced swim and tail suspension tests. We next examined the neuronal mechanism by which subchronic stress decreases passive coping in postpartum female mice. Neuronal activity and expression of noradrenergic receptors in the medial prefrontal cortex (mPFC) were analyzed using immunohistochemistry and reverse transcription-quantitative polymerase chain reaction, respectively. The mPFC was manipulated using chemogenetics, knockdown, or an α2A adrenergic receptor (AR) antagonist. Immunohistochemistry revealed that subchronic restraint stress increased glutamatergic neuron activation in the mPFC via forced swim stress and decreased α2A AR expression in postpartum females. Chemogenetic activation of glutamatergic neurons in the mPFC, knockdown of α2AAR in the mPFC, and the α2A AR receptor antagonist atipamezole treatment decreased passive coping in postpartum females. Subchronic restraint stress decreased passive coping in postpartum females by increasing glutamatergic neuron activity in the mPFC through α2A AR attenuation. The noradrenergic regulation of the mPFC may be a new target for treating postpartum depression.

The Association Between the Frequency of Agriculture and Control of Chronic Diseases Among Regular Patients in Rural Community Hospitals: A Cross-Sectional Study.

Introduction The importance of exercise in chronic disease management among older people is paramount, especially as the global population ages and the prevalence of chronic diseases such as cardiovascular disease, diabetes, and arthritis increases. Regular physical activity enhances cardiovascular health, improves metabolic function, and alleviates symptoms of musculoskeletal disorders. As a form of exercise, agriculture provides physical and mental benefits for older adults. However, its impact on chronic disease management can be mixed, as the physical demands and potential stress associated with agricultural tasks can exacerbate certain health conditions. Method This cross-sectional study was conducted in Unnan City, a rural area in Japan, focusing on residents aged 40 and older who regularly visited Unnan City Hospital. Data were collected from 647 participants between September 1, 2023, and November 31, 2023, through questionnaires and electronic medical records. The primary outcome was the management of chronic diseases, assessed through hypertension, dyslipidemia, diabetes mellitus, and obesity control. Participants reported their frequency of agricultural activities, exercise, eating habits, and sleep. Statistical analyses included t-tests, Mann-Whitney U tests, and multivariate logistic regression models. Results Participants engaging frequently in agricultural activities were younger, had better hypertension control, and reported healthier eating habits and sleep patterns. Frequent agricultural activities were associated with a lower likelihood of hypertension (OR: 0.62, 95% CI: 0.39-0.97, p = 0.034). Older age (≥75 years), higher BMI (≥25), and a Charlson comorbidity index (CCI) score of ≥5 were significantly associated with hypertension. No significant associations were found between hypertension and other health-related variables such as healthy eating, adequate sleep, and regular exercise. Conclusion The study highlights the potential benefits of agricultural activities in managing chronic diseases, particularly hypertension, among older adults. However, the physical demands and possible social isolation associated with agricultural tasks require a nuanced approach to promoting these activities. Tailored, community-based agricultural programs that foster social interaction and support can enhance physical and mental health benefits. Future research should focus on longitudinal studies to confirm these findings and explore the long-term health outcomes of agricultural activities in diverse contexts.

115 The impact of physical stressors in a neonatal intensive care unit in Ireland

115 The impact of physical stressors in a neonatal intensive care unit in Ireland

Model for predicting metabolic activity in athletes based on biochemical blood test analysis

Improving the efficiency of athletic performance and reducing the likelihood of overtraining are primarily determined goals that can be achieved by the correct organization of the training process. The nature of adaptation to physical stress is associated with the specificity, focus, and degree of biochemical and functional changes that occur during muscular work. In this study, we aimed to develop a diagnostic model for predicting metabolic processes in athletes based on standard biochemical blood analysis indicators. The study involved athletes from the track and field athletics team (men, n ​= ​42, average age was [22.55 ​± ​3.68] years). Blood samples were collected in the morning at the beginning and end of the training week during the annual cycle. During the entire period, 3 625 laboratory parameter tests were conducted. Capillary blood sampling in athletes was conducted from the distal phalanx of the finger after overnight fasting, according to standard diagnostic procedures. To determine the predominance of anabolic or catabolic processes, equations were derived from a linear discriminant function. The discriminant function of predicting metabolic processes in athletes has a high information capacity (92.1%), as confirmed by the biochemical results of neuroendocrine system activity, which characterized the body's stage of adaptive regulatory mechanisms in response to stress factors. The classification matrix used to predict the metabolic processes based on the results of the discriminant function calculation demonstrates the statistical significance of the model (p ​< ​0.01). Consequently, an informative mathematical model was developed, which enabled the reliable and timely prediction of the prevalence of one of the metabolic activity phases in the athlete's body. The use of the developed model will also allow us to assess the nature of adaptation to specific muscular work, identify an athlete's weaknesses, forecast the success of their performance, and timely adjust both the training process and the recovery program.

Impact of Caring for A Child With Cancer: Predicting Factors Affecting The Physical Health And Healthy Life Behaviors of The Mother

Purpose: To prevent mothers of children with cancer from developing health problems and suggest effective ways to maintain their health, it is important to accurately determine the problems they face. The aim of this study was to examine the physical health problems and healthy lifestyle behaviors of mothers whose children have cancer. Material and Methods: This methodological, descriptive, and correlational study. The study interviewed 150 mothers whose children were receiving treatment for cancer using three forms: Sociodemographic Information Form, Mothers' Physical Health Form, and Healthy Lifestyle Behaviors Scale II. Independent samples t-test and single way variance analysis (ANOVA) tests were conducted to measure the relations between the variables. Results: Mothers’ age, number of children with cancer, age of child, educational status, income level and marital status were significantly correlated with the survey variables. A model created based on the regression analysis of the relationship among the variables showed that sociodemographic characteristics affected 24.2% of the healthy lifestyle behavior of mothers of children with cancer. Conclusion: Our results showed that physical activity and stress management received the lowest health lifestyle behavior scores. The current findings emphasize the importance of informing mothers about stress management and physical activities during the care of their children with cancer.

Determinant factors associated with central obesity in the academic staff at Universitas Negeri Gorontalo

Central obesity is one of the causes of metabolic syndrome and other degenerative diseases. This study aimed to analyze the relationship between age, gender, physical activity, genetics, stress, and diet with the incidence of central obesity in academic staff at Universitas Negeri Gorontalo. The research method used was an analytical observational method with a cross-sectional study design with the number of research subjects 248 samples consisting of employees or education personnel at Universitas Negeri Gorontalo. Data were collected using a questionnaire and analyzed using binary logistic regression analysis. The results showed that physical activity, genetics, stress, and calorie requirement had a significant relationship with central obesity, while age and gender did not. The calorie requirement variable had a p-value of 0.000 and an odds ratio of 10.75. The physical activity variable had a p-value of 0.009 with an odds ratio of 0.59. The stress variable has a p-value of 0.015 with an odds ratio of 2.09. The results of the data analysis test obtained an R-square value of 0.359. This indicates that the independent variable can explain or influence the dependent variable by 35.9%. The remaining 64.1% is explained by other variables not included in this study.

The impact of soccer-specific psychophysiological stress on inhibition and cognitive flexibility in elite youth players

While researchers and practitioners attribute an essential role to executive functions (EFs) for soccer performance, the usefulness of respective diagnostics and the predictive value remain unclear. One limitation restricting the translation and relevance of study results to improve actual game performance is the insufficient consideration of competitive conditions. Thus, this study aimed to conduct soccer-specific cognitive diagnostics under a soccer-specific psychophysiological stress condition, mimicing the demands of a competitive game. A total of 92 (Mage = 15.17, SDage = 1.45) youth elite players performed tests for inhibition (flanker task) or cognitive flexibility (number-letter task) with a soccer-specific motor response (i.e., pass into goals). After a pre-test in a neutral condition, players were randomly assigned to a neutral (moderate soccer-specific exercise) or a stress condition (physical stress and competitive instructions and filming for psychological stress). Objective (i.e., cortisol, heart rate variability) and subjective stress-related measures (i.e., SAM, VAS) were assessed six times throughout experimental procedure. Analyses revealed significant interaction effects between time and condition for all objective and subjective variables indicating a successful experimental stress induction. For cognitive performance, results revealed significant main effects of time, but no significant interaction effects between time and condition. However, descriptive statistics suggested improved performance under stress, with decreased flanker effect and switch costs. Additionally, response time variability in the flanker task significantly decreased in the stress condition. These findings offer insights into individual stress perception and processing under game-related psychophysiological demands, expanding previous research on situational EF alterations that also hold relevance for applied practitioners.

Takotsubo syndrome: stress cardiomyopathy and its relation to “broken heart”

Takotsubo Syndrome, also known as Takotsubo cardiomyopathy, is a transient cardiac condition characterized by left ventricular dysfunction without coronary obstruction. Predominantly in postmenopausal women, it can be triggered by emotional or physical stress. Its pathophysiology involves hyperactivation of the autonomic nervous system and an increase in catecholamines. Diagnosis is challenging, but specific clinical and anatomical criteria help with differentiation. Treatment focuses on managing acute complications and preventing future events, including managing cardiac dysfunction and controlling catecholamine levels. In conclusion, an interdisciplinary strategy is essential to ensure optimal care for the affected patient, with a focus on timely assistance to benefit the individual with the syndrome.

Socioeconomic Influence on Physical Therapist Student Financial Literacy, Self-Efficacy, Stress, and Anxiety.

Doctor of Physical Therapy (DPT) students have considerable educational debt upon graduation with suspected low levels of financial literacy, limited financial self-efficacy, and elevated stress and anxiety. The purpose of this study was to determine the relationships between financial knowledge, financial anxiety, financial stress, and financial self-efficacy with socioeconomic determinants in DPT students. Five hundred seventy-eight DPT students, surveyed through a cross-sectional sample of convenience. Participants responded to a 40-item demographic questionnaire providing a self-assessment of their current financial literacy, financial self-efficacy, financial anxiety, financial stress, and level of general social support. Pairwise correlations were used to determine the relationship between independent variables and composite scores on self-assessment tools. Multiple regression analyses were conducted to predict financial knowledge, self-efficacy, anxiety, stress, and social support by socioeconomic indicators and educational debt status. While there was a moderate, positive association between presence of debt and financial self-efficacy and financial anxiety for all students (r = .55), there was no statistically significant difference in financial knowledge, self-efficacy, anxiety, stress, or social support for students based on race/ethnicity. There was a negligible to weak correlation between financial knowledge, financial self-efficacy, and level of financial anxiety based on age, race/ethnicity, gender, socioeconomic background, debt load, risk tolerance, and retirement reflection. When subjected to multiple regression analysis, the level of education-related debt could explain a large proportion of the variance in multiple measures, including financial self-efficacy, anxiety, and stress. Economic background explained a large proportion of variance in the general social support students felt. We found a strong, positive correlation between the presence of debt and financial stress and anxiety for all DPT student respondents. While there is no difference in financial literacy and self-efficacy based on race and ethnic background, there is a moderate correlation between self-efficacy and financial anxiety for all students. Education on strategies to manage debt load may reduce both factors.

Healthy lifestyle scales to assess general and clinical population: A systematic review with narrative synthesis

AbstractBackgroundAdopting a healthy lifestyle and improvements in medical care have led to an increase in life expectancy. Thus, the development and implementation of techniques for assessing healthy lifestyles have garnered more attention recently. Finding and evaluating healthy lifestyle assessment tools across all demographics is the goal of this systematic review.MethodUsing online databases PubMed, Scopus, Web of Science, and APA PsycINFO, a systematic literature review was carried out in accordance with Preferred Reporting Items for Systematic Review and Meta‐analyses. Registration DOI https://doi.org/10.17605/OSF.IO/ZD9XK for the review has been set on Open Science Framework (OSF). Using the quality standards proposed for measuring features of health status surveys, two independent reviewers evaluated the methodological quality ratings of the eligible studies.ResultsFrom 7480 studies, 47 eligible instruments were identified and included in the review. Most of the studies followed the World Health Organization's definition of healthy lifestyle to develop the scales. The instruments are categorized into those used to assess general lifestyle or healthy lifestyle in general as well as in the clinical population. Physical activity, nutrition, tobacco use, alcohol use, and stress management were the most assessed modules of healthy lifestyle. Furthermore, validity, reliability, responsiveness, floor and ceiling effects, and interpretability were among the psychometric qualities that were frequently assessed.ConclusionLifestyle questionnaires can be used to identify specific risk factors in a variety of populations, as well as to plan community‐based intervention programs, by acting as a predictive diagnostic tool and preventive measure. The majority of the assessments of healthy lifestyles that are currently accessible require improvements.

The impact of virtual reality scenes on stress response characteristics of individuals with different personality traits

Virtual reality based physical stress (VRPS) paradigms could eliminate the influence of social factors on participants, and it may be a desirable tool to explore the impact of personality traits on stress levels. In this study, we attempt to explore the effects of VRPS on stress response among individuals with different personality traits. Forty male participants with an average age of 22.79 ± 0.41 years were divided into two groups based on Harm Avoidance (HA) scores of Tridimensional Personality Questionnaire (TPQ), referred to as the Low-HA group and the High-HA group. The stress levels of the participants were assessed using salivary α-amylase (sAA) activity and heart rate variability (HRV) indices pre- and post-stress. The influence of personality traits on stress response among different groups was analyzed. VRPS significantly affected the sAA activity and HRV indicators of both groups. During and after stress, there were significant differences in sAA activity and HRV indicators between the two groups. The sAA levels and HRV indices of the Low-HA group were lower than those of the High-HA group. Furthermore, sAA levels and HRV indices were correlated with the scores of TPQ. VRPS scenarios elicit different stress responses on individuals with different harm avoidance personality traits. Stress evaluation based on VR scenarios presents potential in personality trait assessments, particularly for distinguishing between individuals with low and high HA tendencies.

Combining multiple stressors unexpectedly blocks bacterial migration and growth.

In nature, organisms experience combinations of stressors. However, laboratory studies typically simplify reality and focus on the effects of an individual stressor. Here, we use a microfluidic approach to simultaneously provide a physical stressor (shear flow) and a chemical stressor (H 2 O 2 ) to the human pathogen Pseudomonas aeruginosa . By treating cells with levels of flow and H 2 O 2 that commonly co-occur in nature, we discover that previous reports significantly overestimate the H 2 O 2 levels required to block bacterial growth. Specifically, we establish that flow increases H 2 O 2 effectiveness 50-fold, explaining why previous studies lacking flow required much higher concentrations. Using natural H 2 O 2 levels, we identify the core H 2 O 2 regulon, characterize OxyR-mediated dynamic regulation, and dissect the redundant roles of multiple H 2 O 2 scavenging systems. By examining single-cell behavior, we serendipitously discover that the combined effects of H 2 O 2 and flow block pilus-driven surface migration. Thus, our results counter previous studies and reveal that natural levels of H 2 O 2 and flow synergize to restrict bacterial colonization and survival. By studying two stressors at once, our research highlights the limitations of oversimplifying nature and demonstrates that physical and chemical stress can combine to yield unpredictable effects.