The times required for acquisition and transmission of potato leaf roll virus (PLRV), Corium solani Holmes, and for phloem penetration by individual green peach aphids, Myzus persicae (Sulzer), were examined using an electronic monitoring system (EMS). Individual aphids, recorded on the EMS, were transferred from PLRV infected plants to indicator plants after feeding for specific amounts of time. The indicator plants, Physalis pubescens L. and potato, Solanum tuberosum L. var. Russet Burbank, were observed for the expression of PLRV symptoms in the following weeks. The minimum acquisition feeding time was 1.6 min and the minimum transmission feeding time was 2.5 min. The results show a direct relationship between the duration of acquisition feeding time and the probability of aphid infectivity and pathogenicity The data do not show a marked increase in the incidence of PLRV with increasing transmission times. Phloem feeding often occurred in less than 5 min after aphids were placed on plants.
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